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The map label for this gene is ptsN [H]

Identifier: 157963432

GI number: 157963432

Start: 4408922

End: 4409365

Strand: Direct

Name: ptsN [H]

Synonym: Spea_3618

Alternate gene names: 157963432

Gene position: 4408922-4409365 (Clockwise)

Preceding gene: 157963431

Following gene: 157963433

Centisome position: 85.2

GC content: 44.59

Gene sequence:

>444_bases
ATGGAACTAAGTACCATCCTGCAGCCGGAATGCACTACCTGTGCCACTCCGGGCAGTAAGAAAAAGGTATTGGAGTTAAT
CAGTGATATTGCCGCTGTCCAGTACCCTACCCTTTCTTCACAAGAAATTTTTGAAAGTCTCTTGGCGAGAGAAAAAATGG
GTAGCACGGGTATAGGTAACGGTATAGCGATTCCTCACGGAAGGCTGACAAATATAGAGCGCCCTGTTGCGGTTTTAGTT
AAGTGTGAAGATCCAATCGCATTCGACGCTATCGATAAACAACCTGTCGACATTCTATTTGCGCTATTGGTGCCTTCCGA
GCAGTGTGAGCAGCATTTAGCAACACTGTCTCTAATGGCTGAAAAACTCAACGATAAGGCGGTAGTCAAACAGTTGAGAA
AAACCAGTAATGAGTCAGAACTATACCAAGTCATTACCCAGTAA

Upstream 100 bases:

>100_bases
CTTCGGAACATGCGGATATGTATGCCGCAATTGACTCCCTGATCGATAAGCTCGACCGTCAGGTTATTAAACACAAAGAG
AAACTAATTAAACACTAACG

Downstream 100 bases:

>100_bases
CCTCTTACTGAGGTCTTTAGATTCATCACCCACCTAAAAGTAGGTAGTGATAATTTAAAGGCCTAAGTAATTATTACAGC
AATATTATTTCTTATAATCA

Product: PTS IIA-like nitrogen-regulatory protein PtsN

Products: protein histidine; sugar phosphate

Alternate protein names: Enzyme IIA-NTR; Phosphotransferase enzyme IIA component; PTS system EIIA component [H]

Number of amino acids: Translated: 147; Mature: 147

Protein sequence:

>147_residues
MELSTILQPECTTCATPGSKKKVLELISDIAAVQYPTLSSQEIFESLLAREKMGSTGIGNGIAIPHGRLTNIERPVAVLV
KCEDPIAFDAIDKQPVDILFALLVPSEQCEQHLATLSLMAEKLNDKAVVKQLRKTSNESELYQVITQ

Sequences:

>Translated_147_residues
MELSTILQPECTTCATPGSKKKVLELISDIAAVQYPTLSSQEIFESLLAREKMGSTGIGNGIAIPHGRLTNIERPVAVLV
KCEDPIAFDAIDKQPVDILFALLVPSEQCEQHLATLSLMAEKLNDKAVVKQLRKTSNESELYQVITQ
>Mature_147_residues
MELSTILQPECTTCATPGSKKKVLELISDIAAVQYPTLSSQEIFESLLAREKMGSTGIGNGIAIPHGRLTNIERPVAVLV
KCEDPIAFDAIDKQPVDILFALLVPSEQCEQHLATLSLMAEKLNDKAVVKQLRKTSNESELYQVITQ

Specific function: Seems to have a role in regulating nitrogen assimilation [H]

COG id: COG1762

COG function: function code GT; Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1789597, Length=148, Percent_Identity=56.0810810810811, Blast_Score=161, Evalue=2e-41,
Organism=Escherichia coli, GI2367327, Length=119, Percent_Identity=28.5714285714286, Blast_Score=64, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR006320 [H]

Pfam domain/function: PF00359 PTS_EIIA_2 [H]

EC number: 2.7.1.69

Molecular weight: Translated: 16079; Mature: 16079

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELSTILQPECTTCATPGSKKKVLELISDIAAVQYPTLSSQEIFESLLAREKMGSTGIGN
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCC
GIAIPHGRLTNIERPVAVLVKCEDPIAFDAIDKQPVDILFALLVPSEQCEQHLATLSLMA
CEECCCCCCCCCCCCEEEEEECCCCCEECCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHH
EKLNDKAVVKQLRKTSNESELYQVITQ
HHCCCHHHHHHHHHCCCHHHHHHHHCC
>Mature Secondary Structure
MELSTILQPECTTCATPGSKKKVLELISDIAAVQYPTLSSQEIFESLLAREKMGSTGIGN
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCC
GIAIPHGRLTNIERPVAVLVKCEDPIAFDAIDKQPVDILFALLVPSEQCEQHLATLSLMA
CEECCCCCCCCCCCCEEEEEECCCCCEECCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHH
EKLNDKAVVKQLRKTSNESELYQVITQ
HHCCCHHHHHHHHHCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Phospho group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2695747 [H]