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The map label for this gene is crp [H]

Identifier: 157963323

GI number: 157963323

Start: 4289106

End: 4289741

Strand: Direct

Name: crp [H]

Synonym: Spea_3509

Alternate gene names: 157963323

Gene position: 4289106-4289741 (Clockwise)

Preceding gene: 157963321

Following gene: 157963324

Centisome position: 82.89

GC content: 41.82

Gene sequence:

>636_bases
ATGGCTCTGATTGGAAAGCCAAAACCAGATCCAACTTTGGAATGGTTTTTATCACACTGTCACATTCATAAATATCCAGC
CAAAAGTACTTTGATCCACGCTGGTGAAGATTCAGATACACTTTACTACATAGTAAAAGGCTCTGTTGCTGTCTTGATTA
AAGACGAAGAAGGTAAAGAGATGATTCTTTCTTACCTTAACCAAGGCGACTTTATCGGTGAGCTAGGTTTATTTGAAGAG
CAAGCAGAACGTACTGCATGGGTTCGTGCTAAGCAAGCTTGTGAAATTGCTGAAATCTCTTACAAGAAGTTCAAGCAGCT
AATTCAGGTTAACCCAGAAATCTTGATGAAGCTTTCATCACAGATGGCTTACCGCCTACACAGCACAAGCCAGAAAGTAG
GTGATCTTGCTTTCCTTGACGTTGCTGGAAGAATTGCACAAACATTGCTTCACCTTGCAAAGCAGCCAGATGCAATGACG
CATCCAGATGGCATGCAGATCAAGATCACTCGTCAAGAGATTGGCCAAATCGTTGGTTGTTCTCGTGAGACAGTAGGTCG
TATCTTGAAAATGCTTGAAGAACAAAACCTTATCCAAGCGCATGGCAAAACTATTGTGGTTTACGGAACACGATAA

Upstream 100 bases:

>100_bases
CAAACTAAGACTTCTATCAAGGTTTTAATAGCTTAATTGACGTAAGATGCTTAATTAGCATACTCTTACTTTATAATGTA
ATCAGCCGAGGAACATAGGC

Downstream 100 bases:

>100_bases
GCCAGTATTAAGCTCGGTTTAAGACAGCTTCTATAAAGTGGCCTGAGATTATCTCAGGCCATTTTTTTTAGGAGTTCTGT
GTGAAACTAGCATTATATCT

Product: cAMP-regulatory protein

Products: NA

Alternate protein names: cAMP receptor protein; cAMP regulatory protein [H]

Number of amino acids: Translated: 211; Mature: 210

Protein sequence:

>211_residues
MALIGKPKPDPTLEWFLSHCHIHKYPAKSTLIHAGEDSDTLYYIVKGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEE
QAERTAWVRAKQACEIAEISYKKFKQLIQVNPEILMKLSSQMAYRLHSTSQKVGDLAFLDVAGRIAQTLLHLAKQPDAMT
HPDGMQIKITRQEIGQIVGCSRETVGRILKMLEEQNLIQAHGKTIVVYGTR

Sequences:

>Translated_211_residues
MALIGKPKPDPTLEWFLSHCHIHKYPAKSTLIHAGEDSDTLYYIVKGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEE
QAERTAWVRAKQACEIAEISYKKFKQLIQVNPEILMKLSSQMAYRLHSTSQKVGDLAFLDVAGRIAQTLLHLAKQPDAMT
HPDGMQIKITRQEIGQIVGCSRETVGRILKMLEEQNLIQAHGKTIVVYGTR
>Mature_210_residues
ALIGKPKPDPTLEWFLSHCHIHKYPAKSTLIHAGEDSDTLYYIVKGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEQ
AERTAWVRAKQACEIAEISYKKFKQLIQVNPEILMKLSSQMAYRLHSTSQKVGDLAFLDVAGRIAQTLLHLAKQPDAMTH
PDGMQIKITRQEIGQIVGCSRETVGRILKMLEEQNLIQAHGKTIVVYGTR

Specific function: This protein complexes with cyclic AMP and binds to specific DNA sites near the promoter to regulate the transcription of several catabolite-sensitive operons. The protein induces a severe bend in the DNA. Acts as a negative regulator of its own synthesis

COG id: COG0664

COG function: function code T; cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789756, Length=209, Percent_Identity=87.0813397129187, Blast_Score=386, Evalue=1e-109,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR018488
- InterPro:   IPR000595
- InterPro:   IPR001808
- InterPro:   IPR012318
- InterPro:   IPR014710
- InterPro:   IPR018335
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00027 cNMP_binding; PF00325 Crp [H]

EC number: NA

Molecular weight: Translated: 23733; Mature: 23602

Theoretical pI: Translated: 7.66; Mature: 7.66

Prosite motif: PS00889 CNMP_BINDING_2 ; PS50042 CNMP_BINDING_3 ; PS00042 HTH_CRP_1 ; PS51063 HTH_CRP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALIGKPKPDPTLEWFLSHCHIHKYPAKSTLIHAGEDSDTLYYIVKGSVAVLIKDEEGKE
CCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEECCCCCCEEEEEEECCEEEEEECCCCHH
MILSYLNQGDFIGELGLFEEQAERTAWVRAKQACEIAEISYKKFKQLIQVNPEILMKLSS
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
QMAYRLHSTSQKVGDLAFLDVAGRIAQTLLHLAKQPDAMTHPDGMQIKITRQEIGQIVGC
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEHHHHHHHHCC
SRETVGRILKMLEEQNLIQAHGKTIVVYGTR
CHHHHHHHHHHHHHCCHHHHCCCEEEEEECC
>Mature Secondary Structure 
ALIGKPKPDPTLEWFLSHCHIHKYPAKSTLIHAGEDSDTLYYIVKGSVAVLIKDEEGKE
CCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEECCCCCCEEEEEEECCEEEEEECCCCHH
MILSYLNQGDFIGELGLFEEQAERTAWVRAKQACEIAEISYKKFKQLIQVNPEILMKLSS
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
QMAYRLHSTSQKVGDLAFLDVAGRIAQTLLHLAKQPDAMTHPDGMQIKITRQEIGQIVGC
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEHHHHHHHHCC
SRETVGRILKMLEEQNLIQAHGKTIVVYGTR
CHHHHHHHHHHHHHCCHHHHCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]