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The map label for this gene is artP [H]

Identifier: 157963211

GI number: 157963211

Start: 4151994

End: 4152731

Strand: Direct

Name: artP [H]

Synonym: Spea_3397

Alternate gene names: 157963211

Gene position: 4151994-4152731 (Clockwise)

Preceding gene: 157963208

Following gene: 157963212

Centisome position: 80.24

GC content: 45.39

Gene sequence:

>738_bases
ATGAAGAAGTCAGTATTGGTTGTAGCAAGCTTAGGGTTAACAGGCGCTTTAATGTTAACTGGCTGCGGTAAGAGTGATGA
TGTACTTGTTGTAGGCACCAATGCTTCATTCCCGCCATTTGAATATGTAGGCGGTGCTAGCGGCGATGAGATAATGGGCT
TCGATATCGATTTAGCTAAGCAAATAGCAAAAGATGCCGGTAAAACCCTCAAAGTTGAAAACATGAATTTTGACTCGCTG
ATTGTTGCGCTCAATGCAGGCAAGGTCGACTTTATCGCCTCGGGCATGACTATTACCCCAGAGCGTCAGGCAAGCGTCGA
TTTCTCAGAGCCTTACTATGAAGCCACTCAGATGGTGTTAGTCAATAAAGATAACAACAGCATCAATAGTCTAGACGACC
TTAAGGGTAAGCATATCGCGGTACAGCTAGGCTCAACCGGCGACATCATGGCCAAGAGCTACAGCCAACAGGTTACCGCC
TTCAACTCAGGCTTCGAAGCCGTGATGGAGCTAAAAAATGCCAAGGTTGATTTGGTGTTGTTTGACAGCGAACCTGCCAT
CAGCTTTTTAGAGAAGAATCCACAGCTAAAAATGGTTGAGCTGGATTTTTCACCTGAGTTTTATGGCTTTGCAGTGGCTA
AGGACAAAGTTGAACTGCTAAACAGCATCAACAGCAGCTTAAGCATCATGAAGCAAAACGGTGGCTATGACGCCCTCGTT
AACAAGCATATGAAGTGA

Upstream 100 bases:

>100_bases
GAATCGAATTTTTACGCATTTTTGATGCATAAGTATTGATTTTGATTTTTATCAGTTTATAGTCAATGCCAGTTGAGTAT
TTTGATTAAGAGAATTGATA

Downstream 100 bases:

>100_bases
GCAGGCAATGATAGATGCAATTAACGCTTCACTGTTTACCCCCATAGGCGAAGATGGCTTAATCGGAATTTACCTTATTC
TTAACGGCCTTAAAGTCACC

Product: extracellular solute-binding protein

Products: ADP; phosphate; L-glutamine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 245; Mature: 245

Protein sequence:

>245_residues
MKKSVLVVASLGLTGALMLTGCGKSDDVLVVGTNASFPPFEYVGGASGDEIMGFDIDLAKQIAKDAGKTLKVENMNFDSL
IVALNAGKVDFIASGMTITPERQASVDFSEPYYEATQMVLVNKDNNSINSLDDLKGKHIAVQLGSTGDIMAKSYSQQVTA
FNSGFEAVMELKNAKVDLVLFDSEPAISFLEKNPQLKMVELDFSPEFYGFAVAKDKVELLNSINSSLSIMKQNGGYDALV
NKHMK

Sequences:

>Translated_245_residues
MKKSVLVVASLGLTGALMLTGCGKSDDVLVVGTNASFPPFEYVGGASGDEIMGFDIDLAKQIAKDAGKTLKVENMNFDSL
IVALNAGKVDFIASGMTITPERQASVDFSEPYYEATQMVLVNKDNNSINSLDDLKGKHIAVQLGSTGDIMAKSYSQQVTA
FNSGFEAVMELKNAKVDLVLFDSEPAISFLEKNPQLKMVELDFSPEFYGFAVAKDKVELLNSINSSLSIMKQNGGYDALV
NKHMK
>Mature_245_residues
MKKSVLVVASLGLTGALMLTGCGKSDDVLVVGTNASFPPFEYVGGASGDEIMGFDIDLAKQIAKDAGKTLKVENMNFDSL
IVALNAGKVDFIASGMTITPERQASVDFSEPYYEATQMVLVNKDNNSINSLDDLKGKHIAVQLGSTGDIMAKSYSQQVTA
FNSGFEAVMELKNAKVDLVLFDSEPAISFLEKNPQLKMVELDFSPEFYGFAVAKDKVELLNSINSSLSIMKQNGGYDALV
NKHMK

Specific function: Part of a binding-protein-dependent transport system for arginine [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787031, Length=246, Percent_Identity=34.5528455284553, Blast_Score=130, Evalue=6e-32,
Organism=Escherichia coli, GI1787085, Length=220, Percent_Identity=35.9090909090909, Blast_Score=130, Evalue=7e-32,
Organism=Escherichia coli, GI1787088, Length=226, Percent_Identity=32.7433628318584, Blast_Score=126, Evalue=1e-30,
Organism=Escherichia coli, GI1788228, Length=223, Percent_Identity=28.6995515695067, Blast_Score=103, Evalue=8e-24,
Organism=Escherichia coli, GI1788649, Length=257, Percent_Identity=29.9610894941634, Blast_Score=94, Evalue=8e-21,
Organism=Escherichia coli, GI1788648, Length=229, Percent_Identity=25.764192139738, Blast_Score=82, Evalue=3e-17,

Paralogues:

None

Copy number: 1440 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 460 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 26402; Mature: 26402

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKSVLVVASLGLTGALMLTGCGKSDDVLVVGTNASFPPFEYVGGASGDEIMGFDIDLAK
CCCCEEEEEECCCCEEEEEEECCCCCCEEEEECCCCCCCHHHCCCCCCCEEEECCHHHHH
QIAKDAGKTLKVENMNFDSLIVALNAGKVDFIASGMTITPERQASVDFSEPYYEATQMVL
HHHHHCCCEEEEECCCCCEEEEEEECCCEEEEECCCEECCCCCCCCCCCCCCCCEEEEEE
VNKDNNSINSLDDLKGKHIAVQLGSTGDIMAKSYSQQVTAFNSGFEAVMELKNAKVDLVL
EECCCCCCCCHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCEEEEEE
FDSEPAISFLEKNPQLKMVELDFSPEFYGFAVAKDKVELLNSINSSLSIMKQNGGYDALV
ECCCCCHHHHHCCCCEEEEEEECCCCEEEEEEHHHHHHHHHHCCCCEEEEECCCCCHHHH
NKHMK
HCCCC
>Mature Secondary Structure
MKKSVLVVASLGLTGALMLTGCGKSDDVLVVGTNASFPPFEYVGGASGDEIMGFDIDLAK
CCCCEEEEEECCCCEEEEEEECCCCCCEEEEECCCCCCCHHHCCCCCCCEEEECCHHHHH
QIAKDAGKTLKVENMNFDSLIVALNAGKVDFIASGMTITPERQASVDFSEPYYEATQMVL
HHHHHCCCEEEEECCCCCEEEEEEECCCEEEEECCCEECCCCCCCCCCCCCCCCEEEEEE
VNKDNNSINSLDDLKGKHIAVQLGSTGDIMAKSYSQQVTAFNSGFEAVMELKNAKVDLVL
EECCCCCCCCHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCEEEEEE
FDSEPAISFLEKNPQLKMVELDFSPEFYGFAVAKDKVELLNSINSSLSIMKQNGGYDALV
ECCCCCHHHHHCCCCEEEEEEECCCCEEEEEEHHHHHHHHHHCCCCEEEEECCCCCHHHH
NKHMK
HCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamine [Periplasm]; H2O [C]

Specific reaction: ATP + L-glutamine [Periplasm] + H2O = ADP + phosphate + L-glutamine [Cytoplasm] [C]

General reaction: Translocates substrate across the membrane [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377 [H]