Click here to switch to the map view.

The map label for this gene is pyrC [H]

Identifier: 157963032

GI number: 157963032

Start: 3941581

End: 3942618

Strand: Direct

Name: pyrC [H]

Synonym: Spea_3215

Alternate gene names: 157963032

Gene position: 3941581-3942618 (Clockwise)

Preceding gene: 157963030

Following gene: 157963033

Centisome position: 76.17

GC content: 50.39

Gene sequence:

>1038_bases
ATGACGCAAATCACCCTACTTACTCCTGACGATTGGCACTTACATTTTCGCGATGGCGATATGTTGCAAGAAACCGTTCC
CGCAACGGCGAGATTGTTCCAACGCGCAATTGTTATGCCGAACCTATTACCACCTGTCACCGACGCCAAGATGGTAACCG
AGTACCGTGAGCGTATTTTAGCGGCGCGACCAGCAGGTTCGACCTTCGAACCGCTAATGACTATCTTCTTGACCAACAAC
ACCACAGAGCAGGACATCATAGATGCCAAAGCTGCTGGCGTTGTTGCAGCAAAGCTTTATCCAGCAGGCGCGACCACTAA
CTCAGATGCTGCAGTTAAAGCTCTCGATGCGCTCTTCCCTGTGTTTGAAGCCATGGCTAAGCACGGCATGTTGTTATTGG
TTCACGGTGAAGTGACTGAATCGCATATCGATATTTTCGACCGCGAAGCCATGTTTATCGAGCGCTACTTAGCCCGTATC
GTTGCCGCCTTCCCAACTCTTAAAGTGGTATTTGAGCACATCACTACTAAAGACGCGGCTGACTTTGTCATGTCGGCTGC
TGACAATGTTGCCGCAACCATTACCCCGCAGCACTTGCTGCTAAACCGCAATGACCTGCTTGTTGGCGGCGTACGTCCGC
ACAACTTCTGTTTGCCAGTGCTAAAACGCAGCACTCACCAAGAGGCGCTACGCGCAGCAGTTGCAACAGGTTCGAGCAAG
TTCTTTTTAGGCACAGACTCAGCGCCACACGAGAAGCACCGCAAAGAGTCGGCATGTGGCTGCGCAGGTTGCTACAGCGC
ATGGAGTGCATTAGAGCTATACGCTCAGGTATTTGACGATCTAGGTGCATTGGACAAGCTTGAAGGTTTTGCTAGCAAGC
ATGGTCCTGACTTTTACGGCTTACCACGCAATACCAGCACTGTTACCCTAGTAAAAGAAAAGTGGACAGTACCGAGCGAG
ATCATCTTGCCAAACGGTAACCCAATAGTGCCTTTCTTCGCTGGTGAAGAGATTAACTGGAAAGTGAAAACCGCTTAA

Upstream 100 bases:

>100_bases
GGTTCAGCTATTAAGCGGCCGCGATTTCAGGCACAATAGCGCCCTAAGAAAAGCTTATAAAAAGCTGACGTATTTTGTCG
TAAACGCTTAGGAACCTACA

Downstream 100 bases:

>100_bases
CAAGCTTAAGAATAGTTAAACTCACGCTTTGGTTAAGAGTAGAAGCCCCTTATTGCGGGCTTTTTTATTTTTGAAGCCAC
TGCATGAGTATCCTTACCCG

Product: dihydroorotase

Products: NA

Alternate protein names: DHOase [H]

Number of amino acids: Translated: 345; Mature: 344

Protein sequence:

>345_residues
MTQITLLTPDDWHLHFRDGDMLQETVPATARLFQRAIVMPNLLPPVTDAKMVTEYRERILAARPAGSTFEPLMTIFLTNN
TTEQDIIDAKAAGVVAAKLYPAGATTNSDAAVKALDALFPVFEAMAKHGMLLLVHGEVTESHIDIFDREAMFIERYLARI
VAAFPTLKVVFEHITTKDAADFVMSAADNVAATITPQHLLLNRNDLLVGGVRPHNFCLPVLKRSTHQEALRAAVATGSSK
FFLGTDSAPHEKHRKESACGCAGCYSAWSALELYAQVFDDLGALDKLEGFASKHGPDFYGLPRNTSTVTLVKEKWTVPSE
IILPNGNPIVPFFAGEEINWKVKTA

Sequences:

>Translated_345_residues
MTQITLLTPDDWHLHFRDGDMLQETVPATARLFQRAIVMPNLLPPVTDAKMVTEYRERILAARPAGSTFEPLMTIFLTNN
TTEQDIIDAKAAGVVAAKLYPAGATTNSDAAVKALDALFPVFEAMAKHGMLLLVHGEVTESHIDIFDREAMFIERYLARI
VAAFPTLKVVFEHITTKDAADFVMSAADNVAATITPQHLLLNRNDLLVGGVRPHNFCLPVLKRSTHQEALRAAVATGSSK
FFLGTDSAPHEKHRKESACGCAGCYSAWSALELYAQVFDDLGALDKLEGFASKHGPDFYGLPRNTSTVTLVKEKWTVPSE
IILPNGNPIVPFFAGEEINWKVKTA
>Mature_344_residues
TQITLLTPDDWHLHFRDGDMLQETVPATARLFQRAIVMPNLLPPVTDAKMVTEYRERILAARPAGSTFEPLMTIFLTNNT
TEQDIIDAKAAGVVAAKLYPAGATTNSDAAVKALDALFPVFEAMAKHGMLLLVHGEVTESHIDIFDREAMFIERYLARIV
AAFPTLKVVFEHITTKDAADFVMSAADNVAATITPQHLLLNRNDLLVGGVRPHNFCLPVLKRSTHQEALRAAVATGSSKF
FLGTDSAPHEKHRKESACGCAGCYSAWSALELYAQVFDDLGALDKLEGFASKHGPDFYGLPRNTSTVTLVKEKWTVPSEI
ILPNGNPIVPFFAGEEINWKVKTA

Specific function: Pyrimidine biosynthesis; third step. [C]

COG id: COG0418

COG function: function code F; Dihydroorotase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHOase family. Type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787301, Length=336, Percent_Identity=57.1428571428571, Blast_Score=393, Evalue=1e-110,
Organism=Saccharomyces cerevisiae, GI6323452, Length=366, Percent_Identity=33.879781420765, Blast_Score=177, Evalue=2e-45,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006680
- InterPro:   IPR004721
- InterPro:   IPR002195 [H]

Pfam domain/function: PF01979 Amidohydro_1 [H]

EC number: =3.5.2.3 [H]

Molecular weight: Translated: 37846; Mature: 37715

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: PS00482 DIHYDROOROTASE_1 ; PS00483 DIHYDROOROTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQITLLTPDDWHLHFRDGDMLQETVPATARLFQRAIVMPNLLPPVTDAKMVTEYRERIL
CCEEEEECCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
AARPAGSTFEPLMTIFLTNNTTEQDIIDAKAAGVVAAKLYPAGATTNSDAAVKALDALFP
HCCCCCCCHHHEEEEEEECCCCHHHHHHHHHHCEEEEEEECCCCCCCCHHHHHHHHHHHH
VFEAMAKHGMLLLVHGEVTESHIDIFDREAMFIERYLARIVAAFPTLKVVFEHITTKDAA
HHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
DFVMSAADNVAATITPQHLLLNRNDLLVGGVRPHNFCLPVLKRSTHQEALRAAVATGSSK
HHHHHHCCCEEEEECHHHEEECCCCEEEECCCCCCHHHHHHHCCHHHHHHHHHHHCCCCE
FFLGTDSAPHEKHRKESACGCAGCYSAWSALELYAQVFDDLGALDKLEGFASKHGPDFYG
EEEECCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCC
LPRNTSTVTLVKEKWTVPSEIILPNGNPIVPFFAGEEINWKVKTA
CCCCCCEEEEEEECCCCCCEEEECCCCEEEEEECCCCCCEEEECC
>Mature Secondary Structure 
TQITLLTPDDWHLHFRDGDMLQETVPATARLFQRAIVMPNLLPPVTDAKMVTEYRERIL
CEEEEECCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
AARPAGSTFEPLMTIFLTNNTTEQDIIDAKAAGVVAAKLYPAGATTNSDAAVKALDALFP
HCCCCCCCHHHEEEEEEECCCCHHHHHHHHHHCEEEEEEECCCCCCCCHHHHHHHHHHHH
VFEAMAKHGMLLLVHGEVTESHIDIFDREAMFIERYLARIVAAFPTLKVVFEHITTKDAA
HHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
DFVMSAADNVAATITPQHLLLNRNDLLVGGVRPHNFCLPVLKRSTHQEALRAAVATGSSK
HHHHHHCCCEEEEECHHHEEECCCCEEEECCCCCCHHHHHHHCCHHHHHHHHHHHCCCCE
FFLGTDSAPHEKHRKESACGCAGCYSAWSALELYAQVFDDLGALDKLEGFASKHGPDFYG
EEEECCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCC
LPRNTSTVTLVKEKWTVPSEIILPNGNPIVPFFAGEEINWKVKTA
CCCCCCEEEEEEECCCCCCEEEECCCCEEEEEECCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA