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The map label for this gene is yafC [C]

Identifier: 157963029

GI number: 157963029

Start: 3937337

End: 3938230

Strand: Direct

Name: yafC [C]

Synonym: Spea_3212

Alternate gene names: 157963029

Gene position: 3937337-3938230 (Clockwise)

Preceding gene: 157963026

Following gene: 157963030

Centisome position: 76.09

GC content: 48.1

Gene sequence:

>894_bases
ATGAATAAACTGTTTGATGGTATCGTGATATTTGTGCAAGTGGTAAAGAGTGCAGGTTTCTCCGCCGCGGCAGAAGTCTT
GGGACACTCCACCTCCTATGTCAGCAAGGAGGTCAATAAACTGGAGTCTAGACTCGGAGTGCGACTGCTCAATAGAACCA
CGCGCTCGATAGGCTTAACCCCTGAGGGAGATGCCTATTATCAGCAGTGCCTACAATTGATTAGCGACGCCGAGCAGGCG
ATAGGCTTAATCACTCAACATGATGTGAGCCCTCGTGGAGTACTAAAACTCAGTTGTCCCGTCGGTTTCGCCCAGCAACA
CCTCAAGCCCATTATCTCCGAATACCTAAAGCGTTACCCCAATGTGAGTTTAGATTTAGATCTAAGTGATAAGCGCACCG
ATGTGATTGCCGATGGTTATGATTTGGCCATTAGAGCATCGGCGCAGCTGGAAGAATCCAGCCTCATCTGCCGTAAGCTC
TATAGCTGTAAAGCTTATACTGTGGCGTCGAAAGAATACATTTCACGCCATGGTAAACCTCACCACCCCAGAGAGCTCGA
GACACATAACGGGATCTGTTACTCCAACCATAAACAGCCAAGCAGATGGGATTACATTGACCATGACGGCAAACACTTTT
CGGTGGATATTAGGCCAAAGATCCTCTGTAACAACGGCAACATGCAGCTAAATCTAGCGCTGGCGGGACATGGCATCACT
CGCTTACCTAACTTCTATATGCAAGGTGAACTCGAAAATGATCAGTTAGAGATCCTGTTTGACAAGTTTCCCAGCGCCAA
TATTGATGTGTTCGTGGTTTACCCAAGCCGCAAACACCTATCGCCCAAAGTCAGAGCCTTTATCGATCTGGCTGCCGAAC
GGTTAGCTGGATAG

Upstream 100 bases:

>100_bases
CCGATGTAGAGCAGTGTATATTAGATGCCAGTTGTGATTAATGGCCTAAAAAGATATTAACTATTTCCATATAACTGTTA
ATCGGCGTCTGGACTCAAGA

Downstream 100 bases:

>100_bases
CCGTTCGACAATGTTAATCTTTATGGCTACTTTCTAGATAAATAGCTTTAAAGTTAACCATCTACAAACTTATAATCGAG
CAGCCCCCTCCCCTGTGGCT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MNKLFDGIVIFVQVVKSAGFSAAAEVLGHSTSYVSKEVNKLESRLGVRLLNRTTRSIGLTPEGDAYYQQCLQLISDAEQA
IGLITQHDVSPRGVLKLSCPVGFAQQHLKPIISEYLKRYPNVSLDLDLSDKRTDVIADGYDLAIRASAQLEESSLICRKL
YSCKAYTVASKEYISRHGKPHHPRELETHNGICYSNHKQPSRWDYIDHDGKHFSVDIRPKILCNNGNMQLNLALAGHGIT
RLPNFYMQGELENDQLEILFDKFPSANIDVFVVYPSRKHLSPKVRAFIDLAAERLAG

Sequences:

>Translated_297_residues
MNKLFDGIVIFVQVVKSAGFSAAAEVLGHSTSYVSKEVNKLESRLGVRLLNRTTRSIGLTPEGDAYYQQCLQLISDAEQA
IGLITQHDVSPRGVLKLSCPVGFAQQHLKPIISEYLKRYPNVSLDLDLSDKRTDVIADGYDLAIRASAQLEESSLICRKL
YSCKAYTVASKEYISRHGKPHHPRELETHNGICYSNHKQPSRWDYIDHDGKHFSVDIRPKILCNNGNMQLNLALAGHGIT
RLPNFYMQGELENDQLEILFDKFPSANIDVFVVYPSRKHLSPKVRAFIDLAAERLAG
>Mature_297_residues
MNKLFDGIVIFVQVVKSAGFSAAAEVLGHSTSYVSKEVNKLESRLGVRLLNRTTRSIGLTPEGDAYYQQCLQLISDAEQA
IGLITQHDVSPRGVLKLSCPVGFAQQHLKPIISEYLKRYPNVSLDLDLSDKRTDVIADGYDLAIRASAQLEESSLICRKL
YSCKAYTVASKEYISRHGKPHHPRELETHNGICYSNHKQPSRWDYIDHDGKHFSVDIRPKILCNNGNMQLNLALAGHGIT
RLPNFYMQGELENDQLEILFDKFPSANIDVFVVYPSRKHLSPKVRAFIDLAAERLAG

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=294, Percent_Identity=34.3537414965986, Blast_Score=168, Evalue=5e-43,
Organism=Escherichia coli, GI1789639, Length=280, Percent_Identity=32.5, Blast_Score=155, Evalue=3e-39,
Organism=Escherichia coli, GI145693193, Length=285, Percent_Identity=31.2280701754386, Blast_Score=146, Evalue=1e-36,
Organism=Escherichia coli, GI1789440, Length=284, Percent_Identity=28.169014084507, Blast_Score=134, Evalue=6e-33,
Organism=Escherichia coli, GI1787128, Length=280, Percent_Identity=26.7857142857143, Blast_Score=134, Evalue=9e-33,
Organism=Escherichia coli, GI87081978, Length=295, Percent_Identity=29.8305084745763, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI1787589, Length=284, Percent_Identity=26.056338028169, Blast_Score=114, Evalue=7e-27,
Organism=Escherichia coli, GI1789173, Length=286, Percent_Identity=26.5734265734266, Blast_Score=78, Evalue=9e-16,
Organism=Escherichia coli, GI1790011, Length=287, Percent_Identity=25.0871080139373, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1788706, Length=186, Percent_Identity=31.1827956989247, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1786448, Length=134, Percent_Identity=31.3432835820896, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI157672245, Length=130, Percent_Identity=30, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33325; Mature: 33325

Theoretical pI: Translated: 8.14; Mature: 8.14

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKLFDGIVIFVQVVKSAGFSAAAEVLGHSTSYVSKEVNKLESRLGVRLLNRTTRSIGLT
CCHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PEGDAYYQQCLQLISDAEQAIGLITQHDVSPRGVLKLSCPVGFAQQHLKPIISEYLKRYP
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCC
NVSLDLDLSDKRTDVIADGYDLAIRASAQLEESSLICRKLYSCKAYTVASKEYISRHGKP
CCEEEEECCCCCCEEEECCCCEEEEECCCCCHHHHHHHHHHCCCEEEEHHHHHHHHCCCC
HHPRELETHNGICYSNHKQPSRWDYIDHDGKHFSVDIRPKILCNNGNMQLNLALAGHGIT
CCCCCCCCCCCEEECCCCCCCCCCCCCCCCCEEEEECCEEEEECCCCEEEEEEEECCCHH
RLPNFYMQGELENDQLEILFDKFPSANIDVFVVYPSRKHLSPKVRAFIDLAAERLAG
HCCCCEEECCCCCCCEEEEEECCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNKLFDGIVIFVQVVKSAGFSAAAEVLGHSTSYVSKEVNKLESRLGVRLLNRTTRSIGLT
CCHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PEGDAYYQQCLQLISDAEQAIGLITQHDVSPRGVLKLSCPVGFAQQHLKPIISEYLKRYP
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCC
NVSLDLDLSDKRTDVIADGYDLAIRASAQLEESSLICRKLYSCKAYTVASKEYISRHGKP
CCEEEEECCCCCCEEEECCCCEEEEECCCCCHHHHHHHHHHCCCEEEEHHHHHHHHCCCC
HHPRELETHNGICYSNHKQPSRWDYIDHDGKHFSVDIRPKILCNNGNMQLNLALAGHGIT
CCCCCCCCCCCEEECCCCCCCCCCCCCCCCCEEEEECCEEEEECCCCEEEEEEEECCCHH
RLPNFYMQGELENDQLEILFDKFPSANIDVFVVYPSRKHLSPKVRAFIDLAAERLAG
HCCCCEEECCCCCCCEEEEEECCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8635745; 7542800 [H]