Click here to switch to the map view.

The map label for this gene is miaB [H]

Identifier: 157962954

GI number: 157962954

Start: 3841468

End: 3842895

Strand: Direct

Name: miaB [H]

Synonym: Spea_3137

Alternate gene names: 157962954

Gene position: 3841468-3842895 (Clockwise)

Preceding gene: 157962953

Following gene: 157962955

Centisome position: 74.24

GC content: 44.47

Gene sequence:

>1428_bases
ATGAGTAAAAAACTCCATATTAAAACTTGGGGCTGTCAAATGAATGAGTATGACTCATCTAAGATGGCTGATCTTCTTGA
CGAATACGAAGGTTACACATTAACAGACAATGCTGAAGAAGCTGACGTGCTGCTTTTAAATACCTGCTCTATTCGTGAGA
AAGCACAGGAGAAAGTGTTTCATCAGCTAGGGCGCTGGAAAACATTAAAAGATAAAAAACCTGGGCTGATTATCGGCGTT
GGTGGTTGTGTTGCCTCACAAGAAGGTAAGGCTATTAAGGAACGTGCTCAGTGCGTAGACCTTATTTTTGGCCCGCAAAC
CTTACATCGTTTACCTGAAATGATCGATCAGATCAAAGACGGTAAAAAAGCGGTTATCGACGTGAGCTTCCCAGAAGTTG
AGAAGTTTGACCGTCTTCCGGAACCGCGGGCCGAAGGTCCAAGTGCTTTCGTATCAATTATGGAAGGCTGTAGCAAATAC
TGCTCTTTCTGTGTGGTGCCTTACACTCGTGGTGAAGAAGTTAGCCGTCCATTGGACGATGTGATTCTTGAAATCGCCCA
ACTCGCAGAGCAAGGCGTGCGTGAAGTTAATCTACTTGGCCAAAACGTAAACGCTTATCGTGGTGCAACCCACGATGATG
AGATCTGTACTTTTGCAGAACTTTTACGTTACGTAGCTGCAATTGATGGTATTGACCGTTTACGCTTTACCACCAGCCAC
CCAATTGAGTTTACGCAAGATATTATTGATGTGTATGAAGACACACCAGAGCTTGTAAGCTTCTTACACTTACCAGTGCA
ATCAGGTTCAGACTTGATCCTAACGCAAATGAAACGTGGACACATGGCAATCGAATATAAGTCGATTATCCGCCGTTTAC
GTAAAGCTCGTCCAGATATCCTAATCAGCTCTGATTTCATCATCGGCTTCCCAGGTGAATCTAAGCAAGACTTCGCTGAT
ACCATGAAGTTGATTGAAGACATACAGTTTGACCATAGCTTTAGCTTTATCTACAGCGCACGCCCAGGAACCCCAGCTGC
GGATCTTCCAGATAATGTTAGTCTTGATGAGAAAAAAGAGCGTCTTGCTATCTTGCAAGACCGTATCACTCAACAAGCTA
TGCGTTATAGCCGTCAAATGGTCGGAACTGTACAACGCATCTTAGTTGAAGGCCCATCGGTTAAGAACCCAATGGAGCTT
CGCGGCCGTACTGAAAACAGTCGTGTGGTAAACTTTGAAGGCCTGCATGAACATATCGGTAAGTTTGTCGACGTAGAGAT
TGTTGACGTATATACCAACTCTCTTCGCGGCGTGTTTATCCGCGGTGAAGATGAAATGGACTTGCGTCGTGATTTACGTC
CATCTGACATCACTGCAAAATATAAGCAAGCTGATGACTTAGGTGTGACCTTGTTTACCCCGGCTTAA

Upstream 100 bases:

>100_bases
AATAATTTCACTTTAATAACGGCTAAATGCTAGCCACAGGCTAACTTAGGCGTTAAAATGTCGCGCTATTTTTTCGGTGC
TTCTAGAGTGATACAGACTG

Downstream 100 bases:

>100_bases
TCCCCGATATTGCTAGGTTATAACACGCGAAAGGCGGCATCCTGAGATGCCGCTTTTTTCTTCTTAGTTGTAACTGGAAA
ATAGCATTCGCATTTATCCA

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase [H]

Number of amino acids: Translated: 475; Mature: 474

Protein sequence:

>475_residues
MSKKLHIKTWGCQMNEYDSSKMADLLDEYEGYTLTDNAEEADVLLLNTCSIREKAQEKVFHQLGRWKTLKDKKPGLIIGV
GGCVASQEGKAIKERAQCVDLIFGPQTLHRLPEMIDQIKDGKKAVIDVSFPEVEKFDRLPEPRAEGPSAFVSIMEGCSKY
CSFCVVPYTRGEEVSRPLDDVILEIAQLAEQGVREVNLLGQNVNAYRGATHDDEICTFAELLRYVAAIDGIDRLRFTTSH
PIEFTQDIIDVYEDTPELVSFLHLPVQSGSDLILTQMKRGHMAIEYKSIIRRLRKARPDILISSDFIIGFPGESKQDFAD
TMKLIEDIQFDHSFSFIYSARPGTPAADLPDNVSLDEKKERLAILQDRITQQAMRYSRQMVGTVQRILVEGPSVKNPMEL
RGRTENSRVVNFEGLHEHIGKFVDVEIVDVYTNSLRGVFIRGEDEMDLRRDLRPSDITAKYKQADDLGVTLFTPA

Sequences:

>Translated_475_residues
MSKKLHIKTWGCQMNEYDSSKMADLLDEYEGYTLTDNAEEADVLLLNTCSIREKAQEKVFHQLGRWKTLKDKKPGLIIGV
GGCVASQEGKAIKERAQCVDLIFGPQTLHRLPEMIDQIKDGKKAVIDVSFPEVEKFDRLPEPRAEGPSAFVSIMEGCSKY
CSFCVVPYTRGEEVSRPLDDVILEIAQLAEQGVREVNLLGQNVNAYRGATHDDEICTFAELLRYVAAIDGIDRLRFTTSH
PIEFTQDIIDVYEDTPELVSFLHLPVQSGSDLILTQMKRGHMAIEYKSIIRRLRKARPDILISSDFIIGFPGESKQDFAD
TMKLIEDIQFDHSFSFIYSARPGTPAADLPDNVSLDEKKERLAILQDRITQQAMRYSRQMVGTVQRILVEGPSVKNPMEL
RGRTENSRVVNFEGLHEHIGKFVDVEIVDVYTNSLRGVFIRGEDEMDLRRDLRPSDITAKYKQADDLGVTLFTPA
>Mature_474_residues
SKKLHIKTWGCQMNEYDSSKMADLLDEYEGYTLTDNAEEADVLLLNTCSIREKAQEKVFHQLGRWKTLKDKKPGLIIGVG
GCVASQEGKAIKERAQCVDLIFGPQTLHRLPEMIDQIKDGKKAVIDVSFPEVEKFDRLPEPRAEGPSAFVSIMEGCSKYC
SFCVVPYTRGEEVSRPLDDVILEIAQLAEQGVREVNLLGQNVNAYRGATHDDEICTFAELLRYVAAIDGIDRLRFTTSHP
IEFTQDIIDVYEDTPELVSFLHLPVQSGSDLILTQMKRGHMAIEYKSIIRRLRKARPDILISSDFIIGFPGESKQDFADT
MKLIEDIQFDHSFSFIYSARPGTPAADLPDNVSLDEKKERLAILQDRITQQAMRYSRQMVGTVQRILVEGPSVKNPMELR
GRTENSRVVNFEGLHEHIGKFVDVEIVDVYTNSLRGVFIRGEDEMDLRRDLRPSDITAKYKQADDLGVTLFTPA

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872784, Length=479, Percent_Identity=35.2818371607516, Blast_Score=278, Evalue=1e-74,
Organism=Homo sapiens, GI28872782, Length=479, Percent_Identity=35.0730688935282, Blast_Score=277, Evalue=2e-74,
Organism=Homo sapiens, GI93277076, Length=397, Percent_Identity=29.9748110831234, Blast_Score=172, Evalue=8e-43,
Organism=Escherichia coli, GI1786882, Length=474, Percent_Identity=73.4177215189873, Blast_Score=729, Evalue=0.0,
Organism=Escherichia coli, GI1787057, Length=430, Percent_Identity=27.2093023255814, Blast_Score=144, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI17553146, Length=461, Percent_Identity=33.1887201735358, Blast_Score=248, Evalue=6e-66,
Organism=Caenorhabditis elegans, GI71996771, Length=314, Percent_Identity=28.0254777070064, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI21356207, Length=437, Percent_Identity=36.1556064073227, Blast_Score=281, Evalue=5e-76,
Organism=Drosophila melanogaster, GI19922432, Length=376, Percent_Identity=30.0531914893617, Blast_Score=154, Evalue=1e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 53757; Mature: 53626

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKKLHIKTWGCQMNEYDSSKMADLLDEYEGYTLTDNAEEADVLLLNTCSIREKAQEKVF
CCCEEEEEEECCCCCCCCHHHHHHHHHHHCCEEECCCCCCCCEEEEECHHHHHHHHHHHH
HQLGRWKTLKDKKPGLIIGVGGCVASQEGKAIKERAQCVDLIFGPQTLHRLPEMIDQIKD
HHHHCCCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCC
GKKAVIDVSFPEVEKFDRLPEPRAEGPSAFVSIMEGCSKYCSFCVVPYTRGEEVSRPLDD
CCEEEEECCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCEEEEECCCCCHHCCCHHH
VILEIAQLAEQGVREVNLLGQNVNAYRGATHDDEICTFAELLRYVAAIDGIDRLRFTTSH
HHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCHHHHHHHHHHHHHHHHCCHHHHEECCCC
PIEFTQDIIDVYEDTPELVSFLHLPVQSGSDLILTQMKRGHMAIEYKSIIRRLRKARPDI
CHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEHHHHHCCCEEEHHHHHHHHHHHCCCCE
LISSDFIIGFPGESKQDFADTMKLIEDIQFDHSFSFIYSARPGTPAADLPDNVSLDEKKE
EEECCEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHH
RLAILQDRITQQAMRYSRQMVGTVQRILVEGPSVKNPMELRGRTENSRVVNFEGLHEHIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCEEEEECHHHHHCC
KFVDVEIVDVYTNSLRGVFIRGEDEMDLRRDLRPSDITAKYKQADDLGVTLFTPA
CCEEEEEEEEECCCCCEEEEECCCCHHHHHCCCCCCCCHHHHCCCCCCEEEECCC
>Mature Secondary Structure 
SKKLHIKTWGCQMNEYDSSKMADLLDEYEGYTLTDNAEEADVLLLNTCSIREKAQEKVF
CCEEEEEEECCCCCCCCHHHHHHHHHHHCCEEECCCCCCCCEEEEECHHHHHHHHHHHH
HQLGRWKTLKDKKPGLIIGVGGCVASQEGKAIKERAQCVDLIFGPQTLHRLPEMIDQIKD
HHHHCCCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCC
GKKAVIDVSFPEVEKFDRLPEPRAEGPSAFVSIMEGCSKYCSFCVVPYTRGEEVSRPLDD
CCEEEEECCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCEEEEECCCCCHHCCCHHH
VILEIAQLAEQGVREVNLLGQNVNAYRGATHDDEICTFAELLRYVAAIDGIDRLRFTTSH
HHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCHHHHHHHHHHHHHHHHCCHHHHEECCCC
PIEFTQDIIDVYEDTPELVSFLHLPVQSGSDLILTQMKRGHMAIEYKSIIRRLRKARPDI
CHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEHHHHHCCCEEEHHHHHHHHHHHCCCCE
LISSDFIIGFPGESKQDFADTMKLIEDIQFDHSFSFIYSARPGTPAADLPDNVSLDEKKE
EEECCEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHH
RLAILQDRITQQAMRYSRQMVGTVQRILVEGPSVKNPMELRGRTENSRVVNFEGLHEHIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCEEEEECHHHHHCC
KFVDVEIVDVYTNSLRGVFIRGEDEMDLRRDLRPSDITAKYKQADDLGVTLFTPA
CCEEEEEEEEECCCCCEEEEECCCCHHHHHCCCCCCCCHHHHCCCCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA