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The map label for this gene is lolB [H]

Identifier: 157962945

GI number: 157962945

Start: 3831568

End: 3832227

Strand: Direct

Name: lolB [H]

Synonym: Spea_3128

Alternate gene names: 157962945

Gene position: 3831568-3832227 (Clockwise)

Preceding gene: 157962935

Following gene: 157962946

Centisome position: 74.05

GC content: 46.52

Gene sequence:

>660_bases
ATGAATAATTTGAGACGCATCACAAAAAACACTTATCTCTGGGTGATACTGGGCACTCTTTTAGTTAGCGGTTGCTCAAC
TACCATACCAGACAACCTAGTTCCCGTCTCTGTTAACAAAGTACAACAGGCTGACGCATGGGAAATGCAAGGTAAGCTTG
CGGTGAGAACGCCCCAAGATAAATTCAGTACTAATCTGTATTGGTTACATAGCCCTGATCATGATGAATTGAAGCTAACC
ACTATGCTTGGAACAACAGTGCTATCACTGTCGAGTCGAGCTGGAGAAGCCACACTAGAAGTCGATGGCAAAACCTACAA
GGATAGCGACCCCCAGCGATTACTCACCCGAGTATCAGGTTGGTCCATACCTATTAAGTCACTGCCACTTTGGATCACAG
GCCAACTAACAGTTGGCGACAAACTGCTAGCCAAAGATGCTCAGGGTCGTCCGGCTAAACTGGTTAATGCCGAACAACAG
CCTCCCTGGGAAGTCGAATTTAACAGCTGGCAAACTCAAAGCGGTGCAGAAGTGCCAAGATTACTTAACCTGCAGCGTGG
TGAGATTCGCCTAAAATTACAGCTGAGTCAGTGGCAAGCTTTAGCACCACAAAGCCAAACGCTTTTCCAACAGACGTCAC
CAACTGAGAACTCTTTATGA

Upstream 100 bases:

>100_bases
TCACGACATTGAGCATTGTATAAATGGTTATTTTTGCATATGGGCATTGGTAAATCATCCAACCCCTCGTATCATAAGGG
CTCTTTCATATCAGTAATAG

Downstream 100 bases:

>100_bases
CAGCGTCACTTTCATTAGGCTGGCCGGCTCCGGCTAAACTAAACCTATTTCTACATATCAACGCTCAGCGTCATGACGGC
TATCACGAACTGCAAACTCT

Product: outer membrane lipoprotein LolB

Products: 5-aminolevulinate; CoA; CO2 [C]

Alternate protein names: NA

Number of amino acids: Translated: 219; Mature: 219

Protein sequence:

>219_residues
MNNLRRITKNTYLWVILGTLLVSGCSTTIPDNLVPVSVNKVQQADAWEMQGKLAVRTPQDKFSTNLYWLHSPDHDELKLT
TMLGTTVLSLSSRAGEATLEVDGKTYKDSDPQRLLTRVSGWSIPIKSLPLWITGQLTVGDKLLAKDAQGRPAKLVNAEQQ
PPWEVEFNSWQTQSGAEVPRLLNLQRGEIRLKLQLSQWQALAPQSQTLFQQTSPTENSL

Sequences:

>Translated_219_residues
MNNLRRITKNTYLWVILGTLLVSGCSTTIPDNLVPVSVNKVQQADAWEMQGKLAVRTPQDKFSTNLYWLHSPDHDELKLT
TMLGTTVLSLSSRAGEATLEVDGKTYKDSDPQRLLTRVSGWSIPIKSLPLWITGQLTVGDKLLAKDAQGRPAKLVNAEQQ
PPWEVEFNSWQTQSGAEVPRLLNLQRGEIRLKLQLSQWQALAPQSQTLFQQTSPTENSL
>Mature_219_residues
MNNLRRITKNTYLWVILGTLLVSGCSTTIPDNLVPVSVNKVQQADAWEMQGKLAVRTPQDKFSTNLYWLHSPDHDELKLT
TMLGTTVLSLSSRAGEATLEVDGKTYKDSDPQRLLTRVSGWSIPIKSLPLWITGQLTVGDKLLAKDAQGRPAKLVNAEQQ
PPWEVEFNSWQTQSGAEVPRLLNLQRGEIRLKLQLSQWQALAPQSQTLFQQTSPTENSL

Specific function: Plays a critical role in the incorporation of lipoproteins in the outer membrane after they are released by the lolA protein [H]

COG id: COG3017

COG function: function code M; Outer membrane lipoprotein involved in outer membrane biogenesis

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lolB family [H]

Homologues:

Organism=Escherichia coli, GI1787460, Length=193, Percent_Identity=26.9430051813472, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004565 [H]

Pfam domain/function: PF03550 LolB [H]

EC number: NA

Molecular weight: Translated: 24518; Mature: 24518

Theoretical pI: Translated: 9.07; Mature: 9.07

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNLRRITKNTYLWVILGTLLVSGCSTTIPDNLVPVSVNKVQQADAWEMQGKLAVRTPQD
CCCHHHHHCCCCCEEHHHHHHHCCCCCCCCCCEEEEEHHHCCCCCCHHCCCEEEEECCCH
KFSTNLYWLHSPDHDELKLTTMLGTTVLSLSSRAGEATLEVDGKTYKDSDPQRLLTRVSG
HHCCCEEEEECCCCCCEEEEEHHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHCC
WSIPIKSLPLWITGQLTVGDKLLAKDAQGRPAKLVNAEQQPPWEVEFNSWQTQSGAEVPR
CCCCHHCCCEEEEEEEEECCHHHHCCCCCCCCEECCCCCCCCEEEEECCCCCCCCCCCCH
LLNLQRGEIRLKLQLSQWQALAPQSQTLFQQTSPTENSL
HHCCCCCEEEEEEEECCCHHCCCCHHHHHHCCCCCCCCC
>Mature Secondary Structure
MNNLRRITKNTYLWVILGTLLVSGCSTTIPDNLVPVSVNKVQQADAWEMQGKLAVRTPQD
CCCHHHHHCCCCCEEHHHHHHHCCCCCCCCCCEEEEEHHHCCCCCCHHCCCEEEEECCCH
KFSTNLYWLHSPDHDELKLTTMLGTTVLSLSSRAGEATLEVDGKTYKDSDPQRLLTRVSG
HHCCCEEEEECCCCCCEEEEEHHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHCC
WSIPIKSLPLWITGQLTVGDKLLAKDAQGRPAKLVNAEQQPPWEVEFNSWQTQSGAEVPR
CCCCHHCCCEEEEEEEEECCHHHHCCCCCCCCEECCCCCCCCEEEEECCCCCCCCCCCCH
LLNLQRGEIRLKLQLSQWQALAPQSQTLFQQTSPTENSL
HHCCCCCEEEEEEEECCCHHCCCCHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Succinyl-CoA; glycine [C]

Specific reaction: Succinyl-CoA + glycine = 5-aminolevulinate + CoA + CO2 [C]

General reaction: Acyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA