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The map label for this gene is rlmG [H]

Identifier: 157962924

GI number: 157962924

Start: 3809096

End: 3810370

Strand: Direct

Name: rlmG [H]

Synonym: Spea_3107

Alternate gene names: 157962924

Gene position: 3809096-3810370 (Clockwise)

Preceding gene: 157962908

Following gene: 157962927

Centisome position: 73.61

GC content: 45.49

Gene sequence:

>1275_bases
ATGCGCCCTAATTTATTTGGTACTTATTGGCATATGACGACACAGTTTTCAGTATCGGGTATTGAACTCGAACTCTTCCG
TTACCCTAGCAGACAAGAGTCTAACCTGCAGGCGTGGGACGCCGCCGATGAGCACCTTATAAAGCACTTAATCGATACAG
AACAAACCAGCATTAGTACGGCGGTTGTGAACGATGGTTTCGGTGCATTAACTGCAGGCTTATTATCAATTAACCCTAGC
TGGCCACTGACATTAGAAACGGATGCCAAGACCAGCCAGTTGGGCACAAGCCAAAACCTAACCCGTAACCAGCTATCGCA
AGAGTCGATAACTTGGGTGAATAGCCGTGATGAGCTGCCTCAGGTAATTGAGTTGGTTTTGATGAAGCTGCCTAAAAACC
TAAACTACTTTAGCCACCAGCTAAATAGGCTTTCTCATGTGCTACCAGCAGGTACACAAGTTTTAATTGGCGCGAAAGCA
AAATCAATTAATAAGTCGCTACTGGAAACTATCGAAAAGAATTTAGGCCCTGCTAGTGCCAGCCTGACATGGAAGAAAAC
TCGAGTCATCACCTGTATCGCAGATGGTAAGGCTCGTGCGTTACCTAAAGCAACCACTTGGTCTGTACCTGAGTTTAAAT
TGCAGATCAGTAATCTCAGTAACGTATTTGCCGCCAACAAGCTCGATATTGGTGCACGCATAATGCTCGATAACATGCCA
AAAGGTGACTACAAGTCGATTGTCGACTTGGGCTGCGGTAACGGCATCTTAGGCTTGCACGCGAAGCAAGTCTTTCCAGA
AGCTTATATTCACTTTATCGATGACTCTGAGATGGCTGTCGCATCGGCTAGAGAAAACTGGGCACTCAACAAACTGGATA
ACCCTGCATTGGTTGGTGAACAAGCAACGTTTGGCTGGGATGACTGTTTAACCAATATGAGTGAAGGCTTCCGCCCAGAT
CTTGTACTGTGTAATCCTCCGTTCCACCAAGGTGAGGCTATTACCGATCATATTGCTTGGCAGATGTTTTTAGATGCCTT
TAGACGCTTAAAAAATGGCGGAATATTGCATGTTGTGGGTAATCGTCACTTGGCCTATCACGTCAAACTGCAGCGGATCT
TTAAGAACTGCACCACGGTTGCATCGAACGGTAAGTTTGTTATTTTACAGGCACAAAAAATCAGTAAAAAGGCACAAGAG
GCTGAGGTGGAGCAAGCTTTTGACACTGAGACGCCACATCCGCAAAGTGCATTATACGGTAAGCCAAAAGCCTAA

Upstream 100 bases:

>100_bases
ATAACTCATACCTAAACTAGGCTCCAAAAAACAATACCTAAGACGGCTCAAACATATCATCACTCTCATCATATACCTGC
TATAAGAAATCTGCGATAAT

Downstream 100 bases:

>100_bases
CCGCTCGCTAAATAGACATAAGCGGTTAAATATAAGCGGTTAAGCAAAAACGGTGAGCCTAGGCTCACCGTTTTGTTTTT
GATTCATTAAATAGTCGCTA

Product: rRNA (guanine-N(2)-)-methyltransferase

Products: NA

Alternate protein names: 23S rRNA m2G1835 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase rlmG [H]

Number of amino acids: Translated: 424; Mature: 424

Protein sequence:

>424_residues
MRPNLFGTYWHMTTQFSVSGIELELFRYPSRQESNLQAWDAADEHLIKHLIDTEQTSISTAVVNDGFGALTAGLLSINPS
WPLTLETDAKTSQLGTSQNLTRNQLSQESITWVNSRDELPQVIELVLMKLPKNLNYFSHQLNRLSHVLPAGTQVLIGAKA
KSINKSLLETIEKNLGPASASLTWKKTRVITCIADGKARALPKATTWSVPEFKLQISNLSNVFAANKLDIGARIMLDNMP
KGDYKSIVDLGCGNGILGLHAKQVFPEAYIHFIDDSEMAVASARENWALNKLDNPALVGEQATFGWDDCLTNMSEGFRPD
LVLCNPPFHQGEAITDHIAWQMFLDAFRRLKNGGILHVVGNRHLAYHVKLQRIFKNCTTVASNGKFVILQAQKISKKAQE
AEVEQAFDTETPHPQSALYGKPKA

Sequences:

>Translated_424_residues
MRPNLFGTYWHMTTQFSVSGIELELFRYPSRQESNLQAWDAADEHLIKHLIDTEQTSISTAVVNDGFGALTAGLLSINPS
WPLTLETDAKTSQLGTSQNLTRNQLSQESITWVNSRDELPQVIELVLMKLPKNLNYFSHQLNRLSHVLPAGTQVLIGAKA
KSINKSLLETIEKNLGPASASLTWKKTRVITCIADGKARALPKATTWSVPEFKLQISNLSNVFAANKLDIGARIMLDNMP
KGDYKSIVDLGCGNGILGLHAKQVFPEAYIHFIDDSEMAVASARENWALNKLDNPALVGEQATFGWDDCLTNMSEGFRPD
LVLCNPPFHQGEAITDHIAWQMFLDAFRRLKNGGILHVVGNRHLAYHVKLQRIFKNCTTVASNGKFVILQAQKISKKAQE
AEVEQAFDTETPHPQSALYGKPKA
>Mature_424_residues
MRPNLFGTYWHMTTQFSVSGIELELFRYPSRQESNLQAWDAADEHLIKHLIDTEQTSISTAVVNDGFGALTAGLLSINPS
WPLTLETDAKTSQLGTSQNLTRNQLSQESITWVNSRDELPQVIELVLMKLPKNLNYFSHQLNRLSHVLPAGTQVLIGAKA
KSINKSLLETIEKNLGPASASLTWKKTRVITCIADGKARALPKATTWSVPEFKLQISNLSNVFAANKLDIGARIMLDNMP
KGDYKSIVDLGCGNGILGLHAKQVFPEAYIHFIDDSEMAVASARENWALNKLDNPALVGEQATFGWDDCLTNMSEGFRPD
LVLCNPPFHQGEAITDHIAWQMFLDAFRRLKNGGILHVVGNRHLAYHVKLQRIFKNCTTVASNGKFVILQAQKISKKAQE
AEVEQAFDTETPHPQSALYGKPKA

Specific function: Specifically methylates the guanosine in position 1835 (m2G1835) of 23S rRNA [H]

COG id: COG2813

COG function: function code J; 16S RNA G1207 methylase RsmC

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmG family [H]

Homologues:

Organism=Escherichia coli, GI87082206, Length=377, Percent_Identity=41.6445623342175, Blast_Score=279, Evalue=3e-76,
Organism=Escherichia coli, GI1790830, Length=364, Percent_Identity=25, Blast_Score=94, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR017237
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: =2.1.1.174 [H]

Molecular weight: Translated: 47087; Mature: 47087

Theoretical pI: Translated: 7.68; Mature: 7.68

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPNLFGTYWHMTTQFSVSGIELELFRYPSRQESNLQAWDAADEHLIKHLIDTEQTSIST
CCCCEEEEEEEEEEEEEECCEEEEEEECCCCCCCCCCHHCCHHHHHHHHHHCCCCCCEEE
AVVNDGFGALTAGLLSINPSWPLTLETDAKTSQLGTSQNLTRNQLSQESITWVNSRDELP
EEECCCCHHHHHHHEEECCCCCEEEECCCCHHHCCCCCCCHHHHHCHHHEEECCCCCCHH
QVIELVLMKLPKNLNYFSHQLNRLSHVLPAGTQVLIGAKAKSINKSLLETIEKNLGPASA
HHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHCCCCCCC
SLTWKKTRVITCIADGKARALPKATTWSVPEFKLQISNLSNVFAANKLDIGARIMLDNMP
EEEEEEEEEEEEEECCCCCCCCCCCCCCCCCEEEEECCCHHHHHCCCCCCCEEEEECCCC
KGDYKSIVDLGCGNGILGLHAKQVFPEAYIHFIDDSEMAVASARENWALNKLDNPALVGE
CCCHHHHHHCCCCCCEECCCHHHHCCHHHEEEECCCCHHHHHHHCCCCCCCCCCCCEECC
QATFGWDDCLTNMSEGFRPDLVLCNPPFHQGEAITDHIAWQMFLDAFRRLKNGGILHVVG
CCCCCHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEC
NRHLAYHVKLQRIFKNCTTVASNGKFVILQAQKISKKAQEAEVEQAFDTETPHPQSALYG
CCEEEEEHHHHHHHHHHHEEECCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCHHCCC
KPKA
CCCC
>Mature Secondary Structure
MRPNLFGTYWHMTTQFSVSGIELELFRYPSRQESNLQAWDAADEHLIKHLIDTEQTSIST
CCCCEEEEEEEEEEEEEECCEEEEEEECCCCCCCCCCHHCCHHHHHHHHHHCCCCCCEEE
AVVNDGFGALTAGLLSINPSWPLTLETDAKTSQLGTSQNLTRNQLSQESITWVNSRDELP
EEECCCCHHHHHHHEEECCCCCEEEECCCCHHHCCCCCCCHHHHHCHHHEEECCCCCCHH
QVIELVLMKLPKNLNYFSHQLNRLSHVLPAGTQVLIGAKAKSINKSLLETIEKNLGPASA
HHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHCCCCCCC
SLTWKKTRVITCIADGKARALPKATTWSVPEFKLQISNLSNVFAANKLDIGARIMLDNMP
EEEEEEEEEEEEEECCCCCCCCCCCCCCCCCEEEEECCCHHHHHCCCCCCCEEEEECCCC
KGDYKSIVDLGCGNGILGLHAKQVFPEAYIHFIDDSEMAVASARENWALNKLDNPALVGE
CCCHHHHHHCCCCCCEECCCHHHHCCHHHEEEECCCCHHHHHHHCCCCCCCCCCCCEECC
QATFGWDDCLTNMSEGFRPDLVLCNPPFHQGEAITDHIAWQMFLDAFRRLKNGGILHVVG
CCCCCHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEC
NRHLAYHVKLQRIFKNCTTVASNGKFVILQAQKISKKAQEAEVEQAFDTETPHPQSALYG
CCEEEEEHHHHHHHHHHHEEECCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCHHCCC
KPKA
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA