Click here to switch to the map view.

The map label for this gene is frmA [H]

Identifier: 157962839

GI number: 157962839

Start: 3703107

End: 3704237

Strand: Direct

Name: frmA [H]

Synonym: Spea_3021

Alternate gene names: 157962839

Gene position: 3703107-3704237 (Clockwise)

Preceding gene: 157962838

Following gene: 157962841

Centisome position: 71.56

GC content: 46.42

Gene sequence:

>1131_bases
ATGACAGCAAAAACAATCAAATCAAAAGCCGCAGTTGCTTGGGCCGTTGGCGAGCCACTAACGATGGAAATCGTTGATGT
TATGCCACCTCAAAAGGGCGAAGTGCGTATTAAGATGATTGCAACAGGTGTATGTCACACAGACGCATTCACCCTTTCCG
GTGACGATCCTGAAGGGATCTTCCCATGTATTCTTGGTCATGAAGGTGGCGGTATCGTCGAATCTATTGGCGAAGGTGTT
ACGAGCGTTAAAGTGGGCGATCACGTGATCCCTCTTTATACTCCAGAATGTGGTGAGTGTAAGTACTGTAAGTCTGGTAA
GACAAACCTTTGCCAGCAAATCCGTGAAACTCAAGGTAAAGGCTTAATGCCTGATGGCACCACTCGCTTTTCAAAAGATG
GCGTAGATATTTTCCATTACATGGGCACCTCGACTTTCTCTGAATACACAGTATTACCTGAGATTTCACTCGCGAAAGTG
AATCCTGATGCACCACTTGAAGAAGTATGTCTACTAGGTTGTGGTGTAACAACGGGTATGGGCGCGGTAATGAACACCGC
CAAAGTAGAAGAAGGCTCAACTGTTGCAGTATTTGGCATGGGCGGTATTGGCTTGTCAGCCATTATCGGTGCAGTGATGG
CTAAAGCGTCACGTATTATTGCTATCGACATTAATGAAAGCAAGTTTGAGCTAGCTCGTAAGCTTGGCGCAACAGACTGC
ATCAACCCAAAAGACTACGATAAGCCTATTCAAGAAGTGATCATTGAGCTCACTGACGGTGGTGTTGATTACTCATTTGA
GTGTATCGGTAACGTTCATGTAATGCGTAGTGCACTTGAGTGCTGCCATAAAGGCTGGGGCGAGTCGGTTGTTATCGGTG
TTGCTGGTGCAGGCCAAGAGATCTCAACTCGTCCATTCCAACTTGTGACCGGTCGAGTATGGAAAGGTAGTGCATTTGGT
GGCGTTAAAGGCCGCTCTGAACTGCCAGAATATGTAGAGCGTTACATGGCGGGCGAATTCAAGTTAGATGACTTCATCAC
TCATACTATGGGGCTTGAGCAAGTAAACGACGCGTTTGACCTAATGCACGAAGGTAAGAGTATCCGTACTGTGCTTCACT
TTGACAAATAG

Upstream 100 bases:

>100_bases
TTTAAGACTGATTATCATGAAAATGAAATCAATTATAATTTTTCACACTAACGACGGTGGTAAGCCGTACTTCTAAATTC
ACTTACAGTAGGAATAAAAG

Downstream 100 bases:

>100_bases
GTAATCGACTAAACAATAGGCGCTCTCGGGCGCTTATTGTTTTCAATACCTATCGGCATAAATTCGCCATAAAAAAGCTC
GATATTTCCCCTAAATCGAT

Product: S-(hydroxymethyl)glutathione dehydrogenase

Products: NA

Alternate protein names: Alcohol dehydrogenase class-3; Alcohol dehydrogenase class-III; Glutathione-dependent formaldehyde dehydrogenase; FALDH; FDH; GSH-FDH [H]

Number of amino acids: Translated: 376; Mature: 375

Protein sequence:

>376_residues
MTAKTIKSKAAVAWAVGEPLTMEIVDVMPPQKGEVRIKMIATGVCHTDAFTLSGDDPEGIFPCILGHEGGGIVESIGEGV
TSVKVGDHVIPLYTPECGECKYCKSGKTNLCQQIRETQGKGLMPDGTTRFSKDGVDIFHYMGTSTFSEYTVLPEISLAKV
NPDAPLEEVCLLGCGVTTGMGAVMNTAKVEEGSTVAVFGMGGIGLSAIIGAVMAKASRIIAIDINESKFELARKLGATDC
INPKDYDKPIQEVIIELTDGGVDYSFECIGNVHVMRSALECCHKGWGESVVIGVAGAGQEISTRPFQLVTGRVWKGSAFG
GVKGRSELPEYVERYMAGEFKLDDFITHTMGLEQVNDAFDLMHEGKSIRTVLHFDK

Sequences:

>Translated_376_residues
MTAKTIKSKAAVAWAVGEPLTMEIVDVMPPQKGEVRIKMIATGVCHTDAFTLSGDDPEGIFPCILGHEGGGIVESIGEGV
TSVKVGDHVIPLYTPECGECKYCKSGKTNLCQQIRETQGKGLMPDGTTRFSKDGVDIFHYMGTSTFSEYTVLPEISLAKV
NPDAPLEEVCLLGCGVTTGMGAVMNTAKVEEGSTVAVFGMGGIGLSAIIGAVMAKASRIIAIDINESKFELARKLGATDC
INPKDYDKPIQEVIIELTDGGVDYSFECIGNVHVMRSALECCHKGWGESVVIGVAGAGQEISTRPFQLVTGRVWKGSAFG
GVKGRSELPEYVERYMAGEFKLDDFITHTMGLEQVNDAFDLMHEGKSIRTVLHFDK
>Mature_375_residues
TAKTIKSKAAVAWAVGEPLTMEIVDVMPPQKGEVRIKMIATGVCHTDAFTLSGDDPEGIFPCILGHEGGGIVESIGEGVT
SVKVGDHVIPLYTPECGECKYCKSGKTNLCQQIRETQGKGLMPDGTTRFSKDGVDIFHYMGTSTFSEYTVLPEISLAKVN
PDAPLEEVCLLGCGVTTGMGAVMNTAKVEEGSTVAVFGMGGIGLSAIIGAVMAKASRIIAIDINESKFELARKLGATDCI
NPKDYDKPIQEVIIELTDGGVDYSFECIGNVHVMRSALECCHKGWGESVVIGVAGAGQEISTRPFQLVTGRVWKGSAFGG
VKGRSELPEYVERYMAGEFKLDDFITHTMGLEQVNDAFDLMHEGKSIRTVLHFDK

Specific function: Has High Formaldehyde Dehydrogenase Activity In The Presence Of Glutathione And Catalyzes The Oxidation Of Normal Alcohols In A Reaction That Is Not Gsh-Dependent. In Addition, Hemithiolacetals Other Than Those Formed From Gsh, Including Omega-Thiol Fatty

COG id: COG1062

COG function: function code C; Zn-dependent alcohol dehydrogenases, class III

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Class-III subfamily [H]

Homologues:

Organism=Homo sapiens, GI71565154, Length=372, Percent_Identity=66.3978494623656, Blast_Score=516, Evalue=1e-147,
Organism=Homo sapiens, GI34577061, Length=371, Percent_Identity=54.4474393530997, Blast_Score=426, Evalue=1e-119,
Organism=Homo sapiens, GI4501929, Length=371, Percent_Identity=52.8301886792453, Blast_Score=410, Evalue=1e-114,
Organism=Homo sapiens, GI4501933, Length=371, Percent_Identity=52.2911051212938, Blast_Score=410, Evalue=1e-114,
Organism=Homo sapiens, GI71565152, Length=375, Percent_Identity=52.8, Blast_Score=404, Evalue=1e-112,
Organism=Homo sapiens, GI156523966, Length=371, Percent_Identity=49.8652291105121, Blast_Score=387, Evalue=1e-108,
Organism=Homo sapiens, GI71743840, Length=371, Percent_Identity=50.4043126684636, Blast_Score=382, Evalue=1e-106,
Organism=Homo sapiens, GI262073058, Length=372, Percent_Identity=50.2688172043011, Blast_Score=381, Evalue=1e-106,
Organism=Homo sapiens, GI4501939, Length=365, Percent_Identity=49.8630136986301, Blast_Score=381, Evalue=1e-106,
Organism=Escherichia coli, GI1786552, Length=369, Percent_Identity=70.7317073170732, Blast_Score=536, Evalue=1e-153,
Organism=Escherichia coli, GI1790045, Length=362, Percent_Identity=27.3480662983425, Blast_Score=104, Evalue=9e-24,
Organism=Escherichia coli, GI1787863, Length=357, Percent_Identity=26.3305322128852, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI87082125, Length=368, Percent_Identity=27.7173913043478, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1788073, Length=351, Percent_Identity=26.7806267806268, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI87081918, Length=227, Percent_Identity=32.15859030837, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1788075, Length=369, Percent_Identity=25.4742547425474, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788407, Length=231, Percent_Identity=27.2727272727273, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1786825, Length=265, Percent_Identity=26.7924528301887, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1786518, Length=370, Percent_Identity=24.8648648648649, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI226510992, Length=353, Percent_Identity=23.7960339943343, Blast_Score=69, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI25146526, Length=377, Percent_Identity=60.4774535809019, Blast_Score=494, Evalue=1e-140,
Organism=Caenorhabditis elegans, GI71997431, Length=379, Percent_Identity=60.1583113456464, Blast_Score=489, Evalue=1e-139,
Organism=Caenorhabditis elegans, GI17565904, Length=376, Percent_Identity=56.9148936170213, Blast_Score=447, Evalue=1e-126,
Organism=Caenorhabditis elegans, GI17562584, Length=363, Percent_Identity=30.0275482093664, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI71988145, Length=371, Percent_Identity=26.1455525606469, Blast_Score=85, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI17562582, Length=361, Percent_Identity=28.5318559556787, Blast_Score=81, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6320033, Length=377, Percent_Identity=59.6816976127321, Blast_Score=472, Evalue=1e-134,
Organism=Saccharomyces cerevisiae, GI6319621, Length=378, Percent_Identity=30.1587301587302, Blast_Score=99, Evalue=9e-22,
Organism=Saccharomyces cerevisiae, GI6323729, Length=356, Percent_Identity=28.9325842696629, Blast_Score=90, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6323099, Length=379, Percent_Identity=27.9683377308707, Blast_Score=89, Evalue=9e-19,
Organism=Saccharomyces cerevisiae, GI6324486, Length=355, Percent_Identity=26.1971830985915, Blast_Score=87, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6323961, Length=364, Percent_Identity=26.0989010989011, Blast_Score=84, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6322619, Length=364, Percent_Identity=27.1978021978022, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6319955, Length=364, Percent_Identity=27.1978021978022, Blast_Score=77, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6319258, Length=226, Percent_Identity=29.2035398230088, Blast_Score=77, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6319257, Length=237, Percent_Identity=26.1603375527426, Blast_Score=63, Evalue=7e-11,
Organism=Drosophila melanogaster, GI17737895, Length=373, Percent_Identity=65.1474530831099, Blast_Score=510, Evalue=1e-145,
Organism=Drosophila melanogaster, GI17137530, Length=348, Percent_Identity=27.0114942528736, Blast_Score=82, Evalue=6e-16,
Organism=Drosophila melanogaster, GI17737897, Length=257, Percent_Identity=28.0155642023346, Blast_Score=75, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014183
- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: =1.1.1.284; =1.1.1.1 [H]

Molecular weight: Translated: 40302; Mature: 40171

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS00059 ADH_ZINC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAKTIKSKAAVAWAVGEPLTMEIVDVMPPQKGEVRIKMIATGVCHTDAFTLSGDDPEGI
CCCHHHHCCCEEEEECCCCCEEEEEECCCCCCCCEEEEEEEECEEECCEEEECCCCCCCC
FPCILGHEGGGIVESIGEGVTSVKVGDHVIPLYTPECGECKYCKSGKTNLCQQIRETQGK
EEEEEECCCCCHHHHHCCCCEEEEECCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCC
GLMPDGTTRFSKDGVDIFHYMGTSTFSEYTVLPEISLAKVNPDAPLEEVCLLGCGVTTGM
CCCCCCCCCCCCCCCEEEEHHCCCCCCCEEECCCCEEEEECCCCCHHHHHHHHCCHHHHH
GAVMNTAKVEEGSTVAVFGMGGIGLSAIIGAVMAKASRIIAIDINESKFELARKLGATDC
HHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHCCCEEEEEEECCHHHHHHHHCCCCCC
INPKDYDKPIQEVIIELTDGGVDYSFECIGNVHVMRSALECCHKGWGESVVIGVAGAGQE
CCCCCCHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHCCCCCEEEEEEECCCCC
ISTRPFQLVTGRVWKGSAFGGVKGRSELPEYVERYMAGEFKLDDFITHTMGLEQVNDAFD
CCCCCEEEEECEEECCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCHHHHHHHHH
LMHEGKSIRTVLHFDK
HHHCCCCEEEEEEECC
>Mature Secondary Structure 
TAKTIKSKAAVAWAVGEPLTMEIVDVMPPQKGEVRIKMIATGVCHTDAFTLSGDDPEGI
CCHHHHCCCEEEEECCCCCEEEEEECCCCCCCCEEEEEEEECEEECCEEEECCCCCCCC
FPCILGHEGGGIVESIGEGVTSVKVGDHVIPLYTPECGECKYCKSGKTNLCQQIRETQGK
EEEEEECCCCCHHHHHCCCCEEEEECCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCC
GLMPDGTTRFSKDGVDIFHYMGTSTFSEYTVLPEISLAKVNPDAPLEEVCLLGCGVTTGM
CCCCCCCCCCCCCCCEEEEHHCCCCCCCEEECCCCEEEEECCCCCHHHHHHHHCCHHHHH
GAVMNTAKVEEGSTVAVFGMGGIGLSAIIGAVMAKASRIIAIDINESKFELARKLGATDC
HHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHCCCEEEEEEECCHHHHHHHHCCCCCC
INPKDYDKPIQEVIIELTDGGVDYSFECIGNVHVMRSALECCHKGWGESVVIGVAGAGQE
CCCCCCHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHCCCCCEEEEEEECCCCC
ISTRPFQLVTGRVWKGSAFGGVKGRSELPEYVERYMAGEFKLDDFITHTMGLEQVNDAFD
CCCCCEEEEECEEECCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCHHHHHHHHH
LMHEGKSIRTVLHFDK
HHHCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]