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The map label for this gene is leuB [H]

Identifier: 157962798

GI number: 157962798

Start: 3652644

End: 3653654

Strand: Direct

Name: leuB [H]

Synonym: Spea_2980

Alternate gene names: 157962798

Gene position: 3652644-3653654 (Clockwise)

Preceding gene: 157962795

Following gene: 157962803

Centisome position: 70.59

GC content: 46.79

Gene sequence:

>1011_bases
ATGACAAAAAGAACTATAACCGTTATCCCTGGTGATGGGATTGGCCCAAGCATTATTGATTCAGCACTTAAGATCCTTGA
TAAAGCAGGTTGTGATTTTGAGTATGAATTTGCAGATGCGGGTTTAACTGCTTTAGAAAAGCACGGTGAATTATTACCCC
AGCGCACCCTTGAGCTTATCGAAAAAAACCGCATTACTCTGAAAGGCCCTTTGACGACCCCTGTCGGTGAAGGCTTCACA
TCGATAAACGTAAGTCTACGTAAGCAATTCTGCCTTTACGCAAACGTGCGCCCAGTGCTGTCTTTTAAAGGCACACAGGC
TCGTTATGACAATATCGACATCATCACGGTACGTGAAAACACCGAAGGTATGTATTCTGGCCTAGGTCAAACAGTCTCTG
AAGACGGCACTACCGCTGAAGCGACCAGTATCATTACTCGCCAAGGCGCTGAGCAGATCACTACATTTGCCTATGAACTT
GCTCGCAAAGAAGGCCGTAAAAAGGTCACTATCGTACATAAAGCTAACATCATGAAGTCAACTTCAGGCCTGTTCCTGAA
AGTCGCTCGTGAAGTCAGCCTGCGTTACCCAGACATCACTACTGAAGAGATGATTGTTGACGCAACTTGCATGAAGCTGG
TGATGAACCCTGAGAACTTCGACGTGATTGTTACCACTAACCTATTTGGTGACATCTTATCTGATCTATGTGCAGGCCTT
GTAGGCGGCCTAGGTATGGCACCTGGCGCTAACATAGGTAGCGACGCGGCAATCTTTGAAGCGGTACACGGCAGTGCACC
AGATATCGCTGGTAAGAACCTAGCTAACCCAACATCTGTCGTGCTTGCATCGATTCAGATGCTGGAATACTTAGGCATGT
CAGATAAAGCAGAACTGATTCGCGGCGCGATTACTGCAGTGATTGAAGAAGGCGACCGTACTACACGCGATCTTGGTGGT
ACTCACGGTACAACTGATTTCACTCAGGCCGTGATTGAGCGTTTATCTTAA

Upstream 100 bases:

>100_bases
TTAAAATAGATCGAACAAAAAGAAAAAATTCAAGTCACTTACCGTGTCCCAAGACATGGTCAGACAATTGCAGTAACGCC
TAACCGTGGTAGGACTATCC

Downstream 100 bases:

>100_bases
TTCTGTTGAGTGACTAAAAAGGCCCCATGTTTGCACATGGGGCCTTTTTTAATGCTCAGTGCCGTCCTGAGATAAGCCGA
CACTTTCGTCAATATTTACC

Product: isocitrate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; IMDH; Beta-IPM dehydrogenase [H]

Number of amino acids: Translated: 336; Mature: 335

Protein sequence:

>336_residues
MTKRTITVIPGDGIGPSIIDSALKILDKAGCDFEYEFADAGLTALEKHGELLPQRTLELIEKNRITLKGPLTTPVGEGFT
SINVSLRKQFCLYANVRPVLSFKGTQARYDNIDIITVRENTEGMYSGLGQTVSEDGTTAEATSIITRQGAEQITTFAYEL
ARKEGRKKVTIVHKANIMKSTSGLFLKVAREVSLRYPDITTEEMIVDATCMKLVMNPENFDVIVTTNLFGDILSDLCAGL
VGGLGMAPGANIGSDAAIFEAVHGSAPDIAGKNLANPTSVVLASIQMLEYLGMSDKAELIRGAITAVIEEGDRTTRDLGG
THGTTDFTQAVIERLS

Sequences:

>Translated_336_residues
MTKRTITVIPGDGIGPSIIDSALKILDKAGCDFEYEFADAGLTALEKHGELLPQRTLELIEKNRITLKGPLTTPVGEGFT
SINVSLRKQFCLYANVRPVLSFKGTQARYDNIDIITVRENTEGMYSGLGQTVSEDGTTAEATSIITRQGAEQITTFAYEL
ARKEGRKKVTIVHKANIMKSTSGLFLKVAREVSLRYPDITTEEMIVDATCMKLVMNPENFDVIVTTNLFGDILSDLCAGL
VGGLGMAPGANIGSDAAIFEAVHGSAPDIAGKNLANPTSVVLASIQMLEYLGMSDKAELIRGAITAVIEEGDRTTRDLGG
THGTTDFTQAVIERLS
>Mature_335_residues
TKRTITVIPGDGIGPSIIDSALKILDKAGCDFEYEFADAGLTALEKHGELLPQRTLELIEKNRITLKGPLTTPVGEGFTS
INVSLRKQFCLYANVRPVLSFKGTQARYDNIDIITVRENTEGMYSGLGQTVSEDGTTAEATSIITRQGAEQITTFAYELA
RKEGRKKVTIVHKANIMKSTSGLFLKVAREVSLRYPDITTEEMIVDATCMKLVMNPENFDVIVTTNLFGDILSDLCAGLV
GGLGMAPGANIGSDAAIFEAVHGSAPDIAGKNLANPTSVVLASIQMLEYLGMSDKAELIRGAITAVIEEGDRTTRDLGGT
HGTTDFTQAVIERLS

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=335, Percent_Identity=46.865671641791, Blast_Score=318, Evalue=6e-87,
Organism=Homo sapiens, GI4758582, Length=336, Percent_Identity=41.9642857142857, Blast_Score=244, Evalue=9e-65,
Organism=Homo sapiens, GI28178821, Length=337, Percent_Identity=39.7626112759644, Blast_Score=233, Evalue=2e-61,
Organism=Homo sapiens, GI28178838, Length=310, Percent_Identity=42.9032258064516, Blast_Score=232, Evalue=4e-61,
Organism=Homo sapiens, GI28178816, Length=336, Percent_Identity=39.5833333333333, Blast_Score=229, Evalue=3e-60,
Organism=Homo sapiens, GI28178819, Length=198, Percent_Identity=46.4646464646465, Blast_Score=172, Evalue=4e-43,
Organism=Escherichia coli, GI87081683, Length=334, Percent_Identity=38.3233532934132, Blast_Score=199, Evalue=2e-52,
Organism=Escherichia coli, GI1788101, Length=358, Percent_Identity=34.9162011173184, Blast_Score=197, Evalue=1e-51,
Organism=Escherichia coli, GI1787381, Length=362, Percent_Identity=33.9779005524862, Blast_Score=191, Evalue=6e-50,
Organism=Caenorhabditis elegans, GI71986051, Length=332, Percent_Identity=46.0843373493976, Blast_Score=321, Evalue=3e-88,
Organism=Caenorhabditis elegans, GI25144293, Length=338, Percent_Identity=40.2366863905325, Blast_Score=244, Evalue=5e-65,
Organism=Caenorhabditis elegans, GI17550882, Length=341, Percent_Identity=39.8826979472141, Blast_Score=233, Evalue=1e-61,
Organism=Caenorhabditis elegans, GI17505779, Length=335, Percent_Identity=38.8059701492537, Blast_Score=230, Evalue=9e-61,
Organism=Saccharomyces cerevisiae, GI6324709, Length=343, Percent_Identity=44.8979591836735, Blast_Score=293, Evalue=2e-80,
Organism=Saccharomyces cerevisiae, GI6324291, Length=337, Percent_Identity=43.3234421364985, Blast_Score=254, Evalue=1e-68,
Organism=Saccharomyces cerevisiae, GI6322097, Length=349, Percent_Identity=38.6819484240688, Blast_Score=224, Evalue=1e-59,
Organism=Saccharomyces cerevisiae, GI6319830, Length=360, Percent_Identity=30, Blast_Score=138, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24643270, Length=332, Percent_Identity=44.578313253012, Blast_Score=315, Evalue=2e-86,
Organism=Drosophila melanogaster, GI24643268, Length=332, Percent_Identity=44.578313253012, Blast_Score=315, Evalue=3e-86,
Organism=Drosophila melanogaster, GI24661184, Length=340, Percent_Identity=42.3529411764706, Blast_Score=288, Evalue=5e-78,
Organism=Drosophila melanogaster, GI161078637, Length=337, Percent_Identity=37.9821958456973, Blast_Score=229, Evalue=2e-60,
Organism=Drosophila melanogaster, GI161078635, Length=337, Percent_Identity=37.9821958456973, Blast_Score=229, Evalue=2e-60,
Organism=Drosophila melanogaster, GI281362242, Length=348, Percent_Identity=41.9540229885057, Blast_Score=229, Evalue=2e-60,
Organism=Drosophila melanogaster, GI24648872, Length=348, Percent_Identity=41.9540229885057, Blast_Score=229, Evalue=2e-60,
Organism=Drosophila melanogaster, GI161078633, Length=337, Percent_Identity=37.9821958456973, Blast_Score=229, Evalue=3e-60,
Organism=Drosophila melanogaster, GI24650122, Length=337, Percent_Identity=37.9821958456973, Blast_Score=229, Evalue=3e-60,
Organism=Drosophila melanogaster, GI161078639, Length=332, Percent_Identity=38.2530120481928, Blast_Score=225, Evalue=4e-59,
Organism=Drosophila melanogaster, GI20130355, Length=331, Percent_Identity=29.0030211480363, Blast_Score=169, Evalue=3e-42,
Organism=Drosophila melanogaster, GI45550436, Length=223, Percent_Identity=25.1121076233184, Blast_Score=91, Evalue=1e-18,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR011828 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 36111; Mature: 35980

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKRTITVIPGDGIGPSIIDSALKILDKAGCDFEYEFADAGLTALEKHGELLPQRTLELI
CCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEHHHCCHHHHHHHHHHHHHHHHHHH
EKNRITLKGPLTTPVGEGFTSINVSLRKQFCLYANVRPVLSFKGTQARYDNIDIITVREN
HCCCEEEECCCCCCCCCCCEEEEHHHHHHHHHHCCCCCCEEECCCCCCCCCEEEEEEECC
TEGMYSGLGQTVSEDGTTAEATSIITRQGAEQITTFAYELARKEGRKKVTIVHKANIMKS
CCHHHHHCCCHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEEEEECCHHHC
TSGLFLKVAREVSLRYPDITTEEMIVDATCMKLVMNPENFDVIVTTNLFGDILSDLCAGL
CCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHH
VGGLGMAPGANIGSDAAIFEAVHGSAPDIAGKNLANPTSVVLASIQMLEYLGMSDKAELI
HCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHH
RGAITAVIEEGDRTTRDLGGTHGTTDFTQAVIERLS
HHHHHHHHHCCCCHHHHCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
TKRTITVIPGDGIGPSIIDSALKILDKAGCDFEYEFADAGLTALEKHGELLPQRTLELI
CCCEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEHHHCCHHHHHHHHHHHHHHHHHHH
EKNRITLKGPLTTPVGEGFTSINVSLRKQFCLYANVRPVLSFKGTQARYDNIDIITVREN
HCCCEEEECCCCCCCCCCCEEEEHHHHHHHHHHCCCCCCEEECCCCCCCCCEEEEEEECC
TEGMYSGLGQTVSEDGTTAEATSIITRQGAEQITTFAYELARKEGRKKVTIVHKANIMKS
CCHHHHHCCCHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEEEEECCHHHC
TSGLFLKVAREVSLRYPDITTEEMIVDATCMKLVMNPENFDVIVTTNLFGDILSDLCAGL
CCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHH
VGGLGMAPGANIGSDAAIFEAVHGSAPDIAGKNLANPTSVVLASIQMLEYLGMSDKAELI
HCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHH
RGAITAVIEEGDRTTRDLGGTHGTTDFTQAVIERLS
HHHHHHHHHCCCCHHHHCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]