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The map label for this gene is queF [H]

Identifier: 157962721

GI number: 157962721

Start: 3538038

End: 3538910

Strand: Direct

Name: queF [H]

Synonym: Spea_2902

Alternate gene names: 157962721

Gene position: 3538038-3538910 (Clockwise)

Preceding gene: 157962719

Following gene: 157962723

Centisome position: 68.37

GC content: 44.44

Gene sequence:

>873_bases
ATGGAAACCGTATTGATGACACAACATCATGATCCCTATAGTAACGCCCCCGAACTTTCTGAATTAACATTAGGAAAGTC
GACAGGTTATCAAGAGCAGTATGATGCATCTTTACTTCAAGGCGTGCCGCGTAAGTTAAACCGTGATGCAATAGGCCTCA
CTCAAGAGTTGCCATTTCATGGCTGTGATATCTGGACAGGCTACGAGCTCTCTTGGTTAAACGCTAAAGGCAAACCTATG
GTTGCCATCGCCGAGTTCAGCCTAAGTTTTGACAGTAAAAACCTTATTGAGTCTAAGTCATTCAAGCTGTATTTAAACAG
CTACAATCAGACACGATTCGAAAACGCACAAGCGGTACAGGAACGACTAGTCCAAGACTTAAGTGCCTGTGCTATGGGTG
ATGTTACCGTTAAGGTTATCGAGCCTAAACAGTTCAGCCACCAGCGCATTGTTGAGCTACCGGGTACTTGCATCGACGAT
CTTGATATTGAGGTTGATGACTATAGCTTCAACCCTGACTACTTAGTTGAAAGCGTCGATAATAAAGCGATTGTTGCAGA
AACGCTGACATCTAACCTGCTTAAATCAAACTGCTTGATCACCTCGCAACCTGACTGGGGCACTGTGATGATCCGCTATC
AAGGGCCAAAGATTGATCGTGAGAAGTTGCTGAGATACCTCATCTCATTCAGACAACATAACGAGTTTCACGAGCAATGT
GTTGAGCGTATTTTTGTTGATCTCAAACGCTTCTGCCAATGTGCCAAGCTGACGGTTTATGCCCGTTATACTCGCCGTGG
TGGTTTAGATATCAATCCATATCGTAGCGATTTTGAACACCCGGCTGAAAATCAACGTCTCGCCAGACAATAA

Upstream 100 bases:

>100_bases
AACGTAGGCTTGTTGATATTTAGATAAAAAAAGATCTAAAGCGGGTAAAGAAGACACTGAAGACAGTTCCAAATCAGTTA
TAATAGGGGTCTATTTTGAC

Downstream 100 bases:

>100_bases
TCGAACTCCAAGCAAAGACCTGTAACGCCCTGCATTACAGGTCTTTTTTATTAAGTTCAAAGCTAAAACTAAGCCTGTGT
TCGCCGTGTTATCGCTTGAT

Product: 7-cyano-7-deazaguanine reductase

Products: NA

Alternate protein names: 7-cyano-7-carbaguanine reductase; NADPH-dependent nitrile oxidoreductase; PreQ(0) reductase [H]

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
METVLMTQHHDPYSNAPELSELTLGKSTGYQEQYDASLLQGVPRKLNRDAIGLTQELPFHGCDIWTGYELSWLNAKGKPM
VAIAEFSLSFDSKNLIESKSFKLYLNSYNQTRFENAQAVQERLVQDLSACAMGDVTVKVIEPKQFSHQRIVELPGTCIDD
LDIEVDDYSFNPDYLVESVDNKAIVAETLTSNLLKSNCLITSQPDWGTVMIRYQGPKIDREKLLRYLISFRQHNEFHEQC
VERIFVDLKRFCQCAKLTVYARYTRRGGLDINPYRSDFEHPAENQRLARQ

Sequences:

>Translated_290_residues
METVLMTQHHDPYSNAPELSELTLGKSTGYQEQYDASLLQGVPRKLNRDAIGLTQELPFHGCDIWTGYELSWLNAKGKPM
VAIAEFSLSFDSKNLIESKSFKLYLNSYNQTRFENAQAVQERLVQDLSACAMGDVTVKVIEPKQFSHQRIVELPGTCIDD
LDIEVDDYSFNPDYLVESVDNKAIVAETLTSNLLKSNCLITSQPDWGTVMIRYQGPKIDREKLLRYLISFRQHNEFHEQC
VERIFVDLKRFCQCAKLTVYARYTRRGGLDINPYRSDFEHPAENQRLARQ
>Mature_290_residues
METVLMTQHHDPYSNAPELSELTLGKSTGYQEQYDASLLQGVPRKLNRDAIGLTQELPFHGCDIWTGYELSWLNAKGKPM
VAIAEFSLSFDSKNLIESKSFKLYLNSYNQTRFENAQAVQERLVQDLSACAMGDVTVKVIEPKQFSHQRIVELPGTCIDD
LDIEVDDYSFNPDYLVESVDNKAIVAETLTSNLLKSNCLITSQPDWGTVMIRYQGPKIDREKLLRYLISFRQHNEFHEQC
VERIFVDLKRFCQCAKLTVYARYTRRGGLDINPYRSDFEHPAENQRLARQ

Specific function: Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) [H]

COG id: COG0780

COG function: function code R; Enzyme related to GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789158, Length=279, Percent_Identity=62.3655913978495, Blast_Score=355, Evalue=1e-99,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016428
- InterPro:   IPR020602 [H]

Pfam domain/function: PF01227 GTP_cyclohydroI [H]

EC number: =1.7.1.13 [H]

Molecular weight: Translated: 33375; Mature: 33375

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METVLMTQHHDPYSNAPELSELTLGKSTGYQEQYDASLLQGVPRKLNRDAIGLTQELPFH
CCCEEEECCCCCCCCCCCHHHEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
GCDIWTGYELSWLNAKGKPMVAIAEFSLSFDSKNLIESKSFKLYLNSYNQTRFENAQAVQ
CCCEECCCEEEEECCCCCCEEEEEEEEECCCCCHHHCCCCEEEEEECCCCHHHHHHHHHH
ERLVQDLSACAMGDVTVKVIEPKQFSHQRIVELPGTCIDDLDIEVDDYSFNPDYLVESVD
HHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHCCCCCCCCCCEEECCCCCCHHHHHHCCC
NKAIVAETLTSNLLKSNCLITSQPDWGTVMIRYQGPKIDREKLLRYLISFRQHNEFHEQC
CCEEHHHHHHHHHHHCCCEEECCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCHHHHHH
VERIFVDLKRFCQCAKLTVYARYTRRGGLDINPYRSDFEHPAENQRLARQ
HHHHHHHHHHHHHHHHHHEEEEEHHCCCCCCCCCCCCCCCCCCCHHHCCC
>Mature Secondary Structure
METVLMTQHHDPYSNAPELSELTLGKSTGYQEQYDASLLQGVPRKLNRDAIGLTQELPFH
CCCEEEECCCCCCCCCCCHHHEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
GCDIWTGYELSWLNAKGKPMVAIAEFSLSFDSKNLIESKSFKLYLNSYNQTRFENAQAVQ
CCCEECCCEEEEECCCCCCEEEEEEEEECCCCCHHHCCCCEEEEEECCCCHHHHHHHHHH
ERLVQDLSACAMGDVTVKVIEPKQFSHQRIVELPGTCIDDLDIEVDDYSFNPDYLVESVD
HHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHCCCCCCCCCCEEECCCCCCHHHHHHCCC
NKAIVAETLTSNLLKSNCLITSQPDWGTVMIRYQGPKIDREKLLRYLISFRQHNEFHEQC
CCEEHHHHHHHHHHHCCCEEECCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCHHHHHH
VERIFVDLKRFCQCAKLTVYARYTRRGGLDINPYRSDFEHPAENQRLARQ
HHHHHHHHHHHHHHHHHHEEEEEHHCCCCCCCCCCCCCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]