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The map label for this gene is folD [H]

Identifier: 157962504

GI number: 157962504

Start: 3270626

End: 3271480

Strand: Direct

Name: folD [H]

Synonym: Spea_2683

Alternate gene names: 157962504

Gene position: 3270626-3271480 (Clockwise)

Preceding gene: 157962497

Following gene: 157962506

Centisome position: 63.21

GC content: 45.85

Gene sequence:

>855_bases
ATGACAGCCCAAAACATCGATGGTAAAGCTATTGCTCAATCCATTCGAACGCAACTCAAAGATAAAGTCACTGCCCGCAA
AGAGGCAGGAAAAAGAGTCCCTGGTTTAGCCGTAATTCTTGTTGGAGCCGATCCAGCTTCACAAGTTTATGTCGGTAGCA
AACGTAAGGCCTGTGAAGAAGTCGGTTTTATCTCTCGCTCTTATGATTTAGACAGCAGCACTTCTGAAGACGCACTCTTA
TCATTAATTGATGAATGTAACGAAGACCCCACGATTGATGGCATCTTGGTACAATTACCGCTTCCTGAACATATTGAAGA
ATCGAAAGTCATTGAGCGTATTCGTCCAGATAAAGATGTAGACGGTTTCCATCCTTATAATGTCGGTCGCTTAGCCCAGC
GCATTCCGGTGTTGCGTTCGTGTACACCTATGGGGATCATGACACTGCTAAAATCGACTGGTGTCGATACCTTTGGCTTA
GATGCCGTTGTTGTCGGCGCATCTAATATTGTCGGCCGCCCAATGTCGCTAGAGCTATTATTAGCAGGCTGTACAACCAC
AACGTGTCACCGCTTTACTCGAAATCTAGAAGATAAAGTTAGAAGTGCAGATTTAGTCGTTGTTGCAGTTGGTAAGCCGG
GCTTTATTCCTGGTGATTGGATTAAACCTGGCGCAATCGTTATCGATGTGGGTATCAACCGCTTAGAGAGTGGTCAGCTT
GTAGGTGATGTCGAATTTGATGTTGCTTCTCAGCACGCGAGTTTCATTACACCTGTTCCTGGTGGCGTCGGCCCAATGAC
TATCGCAAGCCTGTTAGAAAACACGCTATATGCCTGCGAGCAGTATCACGACTAA

Upstream 100 bases:

>100_bases
GAATTGTTTGTTAACAGTCTAAATCAATGACACCTTTAGCCTGTCCTGTTAAAATGTCGCCGTTTTCCATTATCGCGCCG
TATAAAGGACACCACCCCCA

Downstream 100 bases:

>100_bases
TAGAAGGTTCTAGGTTCTAGGTTCTAGGTTCTAGGTTCTAGCAAAATATTTCAGACCTAGTTACTAAAAGAGATACCAGA
CCTTTCCATCCATGGGGTGT

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase [H]

Number of amino acids: Translated: 284; Mature: 283

Protein sequence:

>284_residues
MTAQNIDGKAIAQSIRTQLKDKVTARKEAGKRVPGLAVILVGADPASQVYVGSKRKACEEVGFISRSYDLDSSTSEDALL
SLIDECNEDPTIDGILVQLPLPEHIEESKVIERIRPDKDVDGFHPYNVGRLAQRIPVLRSCTPMGIMTLLKSTGVDTFGL
DAVVVGASNIVGRPMSLELLLAGCTTTTCHRFTRNLEDKVRSADLVVVAVGKPGFIPGDWIKPGAIVIDVGINRLESGQL
VGDVEFDVASQHASFITPVPGGVGPMTIASLLENTLYACEQYHD

Sequences:

>Translated_284_residues
MTAQNIDGKAIAQSIRTQLKDKVTARKEAGKRVPGLAVILVGADPASQVYVGSKRKACEEVGFISRSYDLDSSTSEDALL
SLIDECNEDPTIDGILVQLPLPEHIEESKVIERIRPDKDVDGFHPYNVGRLAQRIPVLRSCTPMGIMTLLKSTGVDTFGL
DAVVVGASNIVGRPMSLELLLAGCTTTTCHRFTRNLEDKVRSADLVVVAVGKPGFIPGDWIKPGAIVIDVGINRLESGQL
VGDVEFDVASQHASFITPVPGGVGPMTIASLLENTLYACEQYHD
>Mature_283_residues
TAQNIDGKAIAQSIRTQLKDKVTARKEAGKRVPGLAVILVGADPASQVYVGSKRKACEEVGFISRSYDLDSSTSEDALLS
LIDECNEDPTIDGILVQLPLPEHIEESKVIERIRPDKDVDGFHPYNVGRLAQRIPVLRSCTPMGIMTLLKSTGVDTFGLD
AVVVGASNIVGRPMSLELLLAGCTTTTCHRFTRNLEDKVRSADLVVVAVGKPGFIPGDWIKPGAIVIDVGINRLESGQLV
GDVEFDVASQHASFITPVPGGVGPMTIASLLENTLYACEQYHD

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate [H]

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family [H]

Homologues:

Organism=Homo sapiens, GI222136639, Length=298, Percent_Identity=44.6308724832215, Blast_Score=227, Evalue=1e-59,
Organism=Homo sapiens, GI94721354, Length=291, Percent_Identity=43.6426116838488, Blast_Score=216, Evalue=2e-56,
Organism=Homo sapiens, GI222418558, Length=291, Percent_Identity=41.9243986254296, Blast_Score=215, Evalue=4e-56,
Organism=Homo sapiens, GI36796743, Length=198, Percent_Identity=30.3030303030303, Blast_Score=82, Evalue=4e-16,
Organism=Escherichia coli, GI1786741, Length=284, Percent_Identity=71.830985915493, Blast_Score=425, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI17568735, Length=289, Percent_Identity=39.4463667820069, Blast_Score=178, Evalue=2e-45,
Organism=Saccharomyces cerevisiae, GI6321643, Length=301, Percent_Identity=43.1893687707641, Blast_Score=231, Evalue=9e-62,
Organism=Saccharomyces cerevisiae, GI6319558, Length=285, Percent_Identity=44.5614035087719, Blast_Score=226, Evalue=3e-60,
Organism=Saccharomyces cerevisiae, GI6322933, Length=311, Percent_Identity=27.6527331189711, Blast_Score=84, Evalue=4e-17,
Organism=Drosophila melanogaster, GI17136818, Length=297, Percent_Identity=48.8215488215488, Blast_Score=261, Evalue=3e-70,
Organism=Drosophila melanogaster, GI17136816, Length=297, Percent_Identity=48.8215488215488, Blast_Score=261, Evalue=4e-70,
Organism=Drosophila melanogaster, GI24645718, Length=290, Percent_Identity=43.7931034482759, Blast_Score=225, Evalue=3e-59,
Organism=Drosophila melanogaster, GI17137370, Length=290, Percent_Identity=43.7931034482759, Blast_Score=225, Evalue=3e-59,
Organism=Drosophila melanogaster, GI62472483, Length=290, Percent_Identity=43.7931034482759, Blast_Score=224, Evalue=4e-59,
Organism=Drosophila melanogaster, GI45551871, Length=290, Percent_Identity=43.7931034482759, Blast_Score=224, Evalue=4e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631 [H]

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C [H]

EC number: =1.5.1.5; =3.5.4.9 [H]

Molecular weight: Translated: 30494; Mature: 30363

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAQNIDGKAIAQSIRTQLKDKVTARKEAGKRVPGLAVILVGADPASQVYVGSKRKACEE
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCEEECCHHHHHHH
VGFISRSYDLDSSTSEDALLSLIDECNEDPTIDGILVQLPLPEHIEESKVIERIRPDKDV
HHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEECCCHHHHHHHHHHHHHCCCCCC
DGFHPYNVGRLAQRIPVLRSCTPMGIMTLLKSTGVDTFGLDAVVVGASNIVGRPMSLELL
CCCCCCCHHHHHHHCHHHHCCCHHHHHHHHHHCCCCCCCCCEEEECCHHHHCCCCEEEEE
LAGCTTTTCHRFTRNLEDKVRSADLVVVAVGKPGFIPGDWIKPGAIVIDVGINRLESGQL
EECCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCEEEEECCCCCCCCCCE
VGDVEFDVASQHASFITPVPGGVGPMTIASLLENTLYACEQYHD
EEEEEHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TAQNIDGKAIAQSIRTQLKDKVTARKEAGKRVPGLAVILVGADPASQVYVGSKRKACEE
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCEEECCHHHHHHH
VGFISRSYDLDSSTSEDALLSLIDECNEDPTIDGILVQLPLPEHIEESKVIERIRPDKDV
HHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEECCCHHHHHHHHHHHHHCCCCCC
DGFHPYNVGRLAQRIPVLRSCTPMGIMTLLKSTGVDTFGLDAVVVGASNIVGRPMSLELL
CCCCCCCHHHHHHHCHHHHCCCHHHHHHHHHHCCCCCCCCCEEEECCHHHHCCCCEEEEE
LAGCTTTTCHRFTRNLEDKVRSADLVVVAVGKPGFIPGDWIKPGAIVIDVGINRLESGQL
EECCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCEEEEECCCCCCCCCCE
VGDVEFDVASQHASFITPVPGGVGPMTIASLLENTLYACEQYHD
EEEEEHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA