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The map label for this gene is pspF [H]

Identifier: 157962491

GI number: 157962491

Start: 3252614

End: 3253714

Strand: Direct

Name: pspF [H]

Synonym: Spea_2670

Alternate gene names: 157962491

Gene position: 3252614-3253714 (Clockwise)

Preceding gene: 157962484

Following gene: 157962492

Centisome position: 62.86

GC content: 46.96

Gene sequence:

>1101_bases
GTGGGCAATCAATTTCAGCAAGATAATCTTATCGGACAGTCCAATGCCTTATTGGAAGTCCTTGAACATATTTCACAAGT
GGCGCCACTGTCTAAACCGGTGTTAATTATTGGTGAGCGTGGCACAGGTAAAGAGCTTATCGCCGAGCGATTACACTACT
TATCTAAACGCTGGGATCAGAGCTTTATCAAACTTAACTGCTCATCCTTAAGTGAAAACTTACTCGAAAGTGAACTCTTT
GGCCATGACGCTGGTGCATTTACTGGAGCCAGTAAAAAGCATGAAGGTCGTTTTGAACGCGCCGACGGCGGTACGCTGTT
TTTAGATGAGCTCGCTAACACCTCAGGACTCATTCAAGAGAAACTGTTACGGGTCATTGAATACGGCGAGTTTGAACGGG
TCGGGGGGAGTAAAACGGTTCAGACCAACGTGCGTCTTGTATGCGCTGCCAACGAAGATCTTCCGTCTCTGGCTGAAGCG
GGTGAATTTAGACCCGATCTGCTCGATAGACTGGCTTTCGATGTTATTACCCTACCGCCTCTGAGACACAGATCTGAGGA
TATCATGCCGCTCGCAGAGTATTTTGCTATCGGTATGGCAAGACAGCTAAAGCTTGAACTATTTGAAGGTTTTAGCTGCA
ATGCGATCGAGCAGCTTATGGAATACCAGTGGCCTGGTAATATCCGTGAGCTTAAAAACGTCGTCGAGCGTAGCGTCTAT
CGAAATGCCGAGACTAATACCGCAATAGATCAGATTATCATCGACCCATTCGCTTCGCCCTATCGCCCGACAAAGCGGGT
TAAAACCAAAGAGCGTCAGCAAACGGTTTCACCCGAGGTGAATTCAGCCGCAACCAATAGCAGCGCTGAAGTGAGTGCTA
ACCCGAGTGCCGACATGAGCACAAACAATAGTGCAGCGGCTGACGCCAAAGTAAACTTCCCTATCGACTTTAAAACCCAT
TGCGAACAGGGTGAGGTGAGGATCTTAAAACAAGCATTGGAAGCAGGTCAGTTTAATCAAAAGAAAACCGCAGAACTGCT
GGGATTAAGCTACCATCAACTGCGCGGGATCTTAAAGAAATACAACTTGCTGGATAAATAA

Upstream 100 bases:

>100_bases
ACAGGCACTTAAACAATTTATTAACTTTTTCTTTCATTTATAATCTGAGCATCATATTATTATTTTCACCAATTATTAGC
GTGAAAGACTAAAACTTACT

Downstream 100 bases:

>100_bases
TTGCTGTTAGCTAGCAGGCACTGTTAAAATAGCTAATAATGTTCAGTTCAAAAATGACTGCTTAATGAAACAGCTGCTTA
AGAGAATAAAGTTAATCGCT

Product: Fis family transcriptional regulator

Products: NA

Alternate protein names: Phage shock protein F [H]

Number of amino acids: Translated: 366; Mature: 365

Protein sequence:

>366_residues
MGNQFQQDNLIGQSNALLEVLEHISQVAPLSKPVLIIGERGTGKELIAERLHYLSKRWDQSFIKLNCSSLSENLLESELF
GHDAGAFTGASKKHEGRFERADGGTLFLDELANTSGLIQEKLLRVIEYGEFERVGGSKTVQTNVRLVCAANEDLPSLAEA
GEFRPDLLDRLAFDVITLPPLRHRSEDIMPLAEYFAIGMARQLKLELFEGFSCNAIEQLMEYQWPGNIRELKNVVERSVY
RNAETNTAIDQIIIDPFASPYRPTKRVKTKERQQTVSPEVNSAATNSSAEVSANPSADMSTNNSAAADAKVNFPIDFKTH
CEQGEVRILKQALEAGQFNQKKTAELLGLSYHQLRGILKKYNLLDK

Sequences:

>Translated_366_residues
MGNQFQQDNLIGQSNALLEVLEHISQVAPLSKPVLIIGERGTGKELIAERLHYLSKRWDQSFIKLNCSSLSENLLESELF
GHDAGAFTGASKKHEGRFERADGGTLFLDELANTSGLIQEKLLRVIEYGEFERVGGSKTVQTNVRLVCAANEDLPSLAEA
GEFRPDLLDRLAFDVITLPPLRHRSEDIMPLAEYFAIGMARQLKLELFEGFSCNAIEQLMEYQWPGNIRELKNVVERSVY
RNAETNTAIDQIIIDPFASPYRPTKRVKTKERQQTVSPEVNSAATNSSAEVSANPSADMSTNNSAAADAKVNFPIDFKTH
CEQGEVRILKQALEAGQFNQKKTAELLGLSYHQLRGILKKYNLLDK
>Mature_365_residues
GNQFQQDNLIGQSNALLEVLEHISQVAPLSKPVLIIGERGTGKELIAERLHYLSKRWDQSFIKLNCSSLSENLLESELFG
HDAGAFTGASKKHEGRFERADGGTLFLDELANTSGLIQEKLLRVIEYGEFERVGGSKTVQTNVRLVCAANEDLPSLAEAG
EFRPDLLDRLAFDVITLPPLRHRSEDIMPLAEYFAIGMARQLKLELFEGFSCNAIEQLMEYQWPGNIRELKNVVERSVYR
NAETNTAIDQIIIDPFASPYRPTKRVKTKERQQTVSPEVNSAATNSSAEVSANPSADMSTNNSAAADAKVNFPIDFKTHC
EQGEVRILKQALEAGQFNQKKTAELLGLSYHQLRGILKKYNLLDK

Specific function: Transcriptional activator for the phage shock protein (psp) operon (pspABCDE) and pspG gene [H]

COG id: COG1221

COG function: function code KT; Transcriptional regulators containing an AAA-type ATPase domain and a DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI87081872, Length=357, Percent_Identity=57.703081232493, Blast_Score=410, Evalue=1e-116,
Organism=Escherichia coli, GI87082117, Length=231, Percent_Identity=50.2164502164502, Blast_Score=231, Evalue=7e-62,
Organism=Escherichia coli, GI1790437, Length=233, Percent_Identity=48.068669527897, Blast_Score=218, Evalue=5e-58,
Organism=Escherichia coli, GI1788550, Length=363, Percent_Identity=35.2617079889807, Blast_Score=218, Evalue=6e-58,
Organism=Escherichia coli, GI1789087, Length=230, Percent_Identity=48.2608695652174, Blast_Score=213, Evalue=1e-56,
Organism=Escherichia coli, GI1789233, Length=359, Percent_Identity=34.2618384401114, Blast_Score=199, Evalue=3e-52,
Organism=Escherichia coli, GI87082152, Length=263, Percent_Identity=41.06463878327, Blast_Score=190, Evalue=1e-49,
Organism=Escherichia coli, GI1790299, Length=227, Percent_Identity=45.374449339207, Blast_Score=189, Evalue=3e-49,
Organism=Escherichia coli, GI1788905, Length=233, Percent_Identity=42.4892703862661, Blast_Score=185, Evalue=4e-48,
Organism=Escherichia coli, GI1786524, Length=242, Percent_Identity=41.3223140495868, Blast_Score=171, Evalue=1e-43,
Organism=Escherichia coli, GI1787583, Length=248, Percent_Identity=38.7096774193548, Blast_Score=167, Evalue=1e-42,
Organism=Escherichia coli, GI1789828, Length=228, Percent_Identity=35.0877192982456, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI87081858, Length=211, Percent_Identity=31.7535545023697, Blast_Score=97, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR014317 [H]

Pfam domain/function: PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 40855; Mature: 40724

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNQFQQDNLIGQSNALLEVLEHISQVAPLSKPVLIIGERGTGKELIAERLHYLSKRWDQ
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCH
SFIKLNCSSLSENLLESELFGHDAGAFTGASKKHEGRFERADGGTLFLDELANTSGLIQE
HHHEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEHHHHCCCHHHHHH
KLLRVIEYGEFERVGGSKTVQTNVRLVCAANEDLPSLAEAGEFRPDLLDRLAFDVITLPP
HHHHHHHCCCHHHCCCCCEEECCEEEEEECCCCCHHHHHHCCCCHHHHHHHHHHHHCCCC
LRHRSEDIMPLAEYFAIGMARQLKLELFEGFSCNAIEQLMEYQWPGNIRELKNVVERSVY
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHH
RNAETNTAIDQIIIDPFASPYRPTKRVKTKERQQTVSPEVNSAATNSSAEVSANPSADMS
CCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCHHHCCCCCCEECCCCCCCCC
TNNSAAADAKVNFPIDFKTHCEQGEVRILKQALEAGQFNQKKTAELLGLSYHQLRGILKK
CCCCCCCCCEECCCCCHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHH
YNLLDK
HHCCCC
>Mature Secondary Structure 
GNQFQQDNLIGQSNALLEVLEHISQVAPLSKPVLIIGERGTGKELIAERLHYLSKRWDQ
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCH
SFIKLNCSSLSENLLESELFGHDAGAFTGASKKHEGRFERADGGTLFLDELANTSGLIQE
HHHEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEHHHHCCCHHHHHH
KLLRVIEYGEFERVGGSKTVQTNVRLVCAANEDLPSLAEAGEFRPDLLDRLAFDVITLPP
HHHHHHHCCCHHHCCCCCEEECCEEEEEECCCCCHHHHHHCCCCHHHHHHHHHHHHCCCC
LRHRSEDIMPLAEYFAIGMARQLKLELFEGFSCNAIEQLMEYQWPGNIRELKNVVERSVY
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHH
RNAETNTAIDQIIIDPFASPYRPTKRVKTKERQQTVSPEVNSAATNSSAEVSANPSADMS
CCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCHHHCCCCCCEECCCCCCCCC
TNNSAAADAKVNFPIDFKTHCEQGEVRILKQALEAGQFNQKKTAELLGLSYHQLRGILKK
CCCCCCCCCEECCCCCHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHH
YNLLDK
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8606168; 9097039; 9278503; 1712397; 7984428; 7920643 [H]