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The map label for this gene is polB [H]

Identifier: 157962476

GI number: 157962476

Start: 3234846

End: 3237245

Strand: Reverse

Name: polB [H]

Synonym: Spea_2655

Alternate gene names: 157962476

Gene position: 3237245-3234846 (Counterclockwise)

Preceding gene: 157962483

Following gene: 157962475

Centisome position: 62.56

GC content: 45.79

Gene sequence:

>2400_bases
ATGAATTCTAGGTTGAACGAGCAGCAAGTGATTAAAGGTCGAGTCCTGACTCGCCATGCGGTCAACCGACAGGGGGGCCT
GCACCTGCAGTATTTTGTTCAGACCGAGAATGGGCCAGTCACTGTTGAAATTCCTAACCAAGAACATGTGTGCTTTGTTC
AGGCGACAGATGCGACTGCATTACTGGCGAGCTCAGAACTGAGAGGCGTCCGTCGAAGCCCTGTAGCTCTTAGCTGTTTT
GCTAACACGCCGGTCGAGGCGCTATATTGTGTCGATGGCCGCCAGCAGCGAAATTTAAACAGGGCTGCTAAAGATCTTGA
TATCGAACTGTTCGAGTCAGATATTAAAGTAGAACATCGTTTTCTCGTCGAACGTTTTATCGCATTAGATGCAGAGTTTT
ACGGCCATTTTGTTGCAAGAACTGGCACGAATGCAGTATCTCAACAGCAACCACTGTTTATCGCAAGTCGAGCTCGTCAG
GCTGTGTCGACGATAGCGCTTAATGCAATCTCTTTAGATTTTGAGTGTAGCTTCGCTGGGGAGCTGTATTCTGTGGGGCT
GTATGGCCAAACGACTGAAGGTTGCGTGTATCAAAAGGTTATTATGGTTGGTGAGGCCGAAGAGACTGATGCTAACTACA
TTGAATGGGTGGAGAACGAAGCGCAATTAATCAATCAGTTGATTAGTTGGTTTGCTGAGTATGATCCCGACATTATTATA
GGTTGGTCAGTAGTCACATTCGATCTCGCACTGCTTTATCGCCGCGCAAAACATCATGGTATTGCATTAACGATAGGGCG
TGATAATCACCCGCTAGGTTGGAAGGTTGAAGATAAGTATCGCCCTGAAACACTATCGTTGCCTGGGCGTGTGGTACTAG
ACGGTATTGATTGGTTGAAGGCGGCTTTTTATCAATTCGACCGCTATTCATTAGAGTTTGTCTCGCAAGCACTGCTAGAT
GAAGGAAAGGCTATCCACAATGTTGATAACCGTATGGATGAAATCAACGATCTCTTTTTAAACAATAAGCCTGCACTGGC
GCACTATAACCTTACTGATTGCCGACTGGTGTGGGATATTTTCGAAAAAACACACCTCTTTGATTTTGCGATTGAACGAG
CCAAGTTGACTGGCCTTGAGTTGGGGAGGGTAGGGGCCTCGGTGGCTGCTTTTAACAACTTGTATCTGCCTCACTTGCAC
CGTGCGGGTTATGTTGCTCCGAGTAAACCCGCCAGCGATGGCATCGAAAGCCCAGGCGGCTATGTGATGGACTCGGTTCC
GGGCTTATATCAGCATATTTTAGTGTTAGACTTTAAGAGTTTGTATCCTTCAATTATACGCACTTTCTTGATCGACCCTA
AAGGCTTGGTAGAGGGGCTCACTCAAGTTGAAGAGCAAACCGTTAGCGGTTTTCTTGGCGCTCAGTTTAGTCGTGAAAAG
CCGATTCTCCCCATGCTGATCAAAAACTTGTCTGAACAGAGAGAACTAGCCAAGGCACATCATGATGCGCCCTTATCTCA
AGCGATAAAAATTATCATGAACTCCCTATATGGTGTGCTAGGTTCCCGCGGCTGTGTGTTTCATGATGCAAGGCTTGCCA
GTTCGATTACCATGCGTGGTCACGAAATCATGAAGCAAACACGGCTTTGGATCGAGGCTGAAGGCTATAAGGTTATCTAT
GGTGATACTGACTCCACCTTCGTCTGGCTTGGCAATGACTTCGAGCAAGAGCAGGTTGCACAGACCGGCAAATCATTAGC
TGAGAAAGTAAACCAGTTATGGCGCGATAAGCTGACCCAAGAGTTTAAGTTAGAGAGCTTTTTAGAATTAGAGTTTGAAA
CCCACTTTGAACAGTTCTTTATGCCAACATTGAGAGGTTCTGTAGAGGGGTCTAAGAAACGTTATGTTGGGACAAAGCGT
CAAACTTCGGGGGAGTTAGGGTTAGTGTTTAAGGGGATGGAGCAAGTACGTAGTGACTGGTCGCCATTGGCACGGCGAGT
TCAATATGAACTTTACGAGCGCTTATTCCAGCATTTGGATGTAACAACATACCTTAGAGAAATTATCGATAGCCTACATA
GTGGTGAGCTTGATGATGAGCTTATTTTTACTAAGCACTTGAGACGTAATCTAGAGGACTATACGGCTAAATCTGCACCT
CATTTAAAAGTTGCCCGCGCTTTATGTGACATCAACCAAGATCAACGCTTTGCCAAGCGTGGGGCACGAATCGAATATGT
CATGACGTTAAATGGCGCAGAGCCTGCCGAGCAAACGTCGAATTTAATTGACTACGATTATTACCTTTCTCGCCAGTTAG
AGCCTATTGCCGAGCCTATTCTTTCAATTCTGAAAACCAGTTTCAGTGAGGTTGCAACAAACCAGATGTCGTTAATTTAA

Upstream 100 bases:

>100_bases
GAATGCACTAAGCTAATAAATAGTGCTGTACATAATAACAGTGATTACTTAGCCTAAGAAGCGTTTAGTGATTATTAGTG
TATTTAATTGAGTATAAAGT

Downstream 100 bases:

>100_bases
GGTGAGGGTCATTAATTTACTGCTAATTAAATGTTAAGGAATGGTTCAATCCTTTGTCTGTTCTGTGTTTGCTAGTTAAT
TAGGTGCAAAATCTAGCAAA

Product: DNA polymerase II

Products: NA

Alternate protein names: Pol II [H]

Number of amino acids: Translated: 799; Mature: 799

Protein sequence:

>799_residues
MNSRLNEQQVIKGRVLTRHAVNRQGGLHLQYFVQTENGPVTVEIPNQEHVCFVQATDATALLASSELRGVRRSPVALSCF
ANTPVEALYCVDGRQQRNLNRAAKDLDIELFESDIKVEHRFLVERFIALDAEFYGHFVARTGTNAVSQQQPLFIASRARQ
AVSTIALNAISLDFECSFAGELYSVGLYGQTTEGCVYQKVIMVGEAEETDANYIEWVENEAQLINQLISWFAEYDPDIII
GWSVVTFDLALLYRRAKHHGIALTIGRDNHPLGWKVEDKYRPETLSLPGRVVLDGIDWLKAAFYQFDRYSLEFVSQALLD
EGKAIHNVDNRMDEINDLFLNNKPALAHYNLTDCRLVWDIFEKTHLFDFAIERAKLTGLELGRVGASVAAFNNLYLPHLH
RAGYVAPSKPASDGIESPGGYVMDSVPGLYQHILVLDFKSLYPSIIRTFLIDPKGLVEGLTQVEEQTVSGFLGAQFSREK
PILPMLIKNLSEQRELAKAHHDAPLSQAIKIIMNSLYGVLGSRGCVFHDARLASSITMRGHEIMKQTRLWIEAEGYKVIY
GDTDSTFVWLGNDFEQEQVAQTGKSLAEKVNQLWRDKLTQEFKLESFLELEFETHFEQFFMPTLRGSVEGSKKRYVGTKR
QTSGELGLVFKGMEQVRSDWSPLARRVQYELYERLFQHLDVTTYLREIIDSLHSGELDDELIFTKHLRRNLEDYTAKSAP
HLKVARALCDINQDQRFAKRGARIEYVMTLNGAEPAEQTSNLIDYDYYLSRQLEPIAEPILSILKTSFSEVATNQMSLI

Sequences:

>Translated_799_residues
MNSRLNEQQVIKGRVLTRHAVNRQGGLHLQYFVQTENGPVTVEIPNQEHVCFVQATDATALLASSELRGVRRSPVALSCF
ANTPVEALYCVDGRQQRNLNRAAKDLDIELFESDIKVEHRFLVERFIALDAEFYGHFVARTGTNAVSQQQPLFIASRARQ
AVSTIALNAISLDFECSFAGELYSVGLYGQTTEGCVYQKVIMVGEAEETDANYIEWVENEAQLINQLISWFAEYDPDIII
GWSVVTFDLALLYRRAKHHGIALTIGRDNHPLGWKVEDKYRPETLSLPGRVVLDGIDWLKAAFYQFDRYSLEFVSQALLD
EGKAIHNVDNRMDEINDLFLNNKPALAHYNLTDCRLVWDIFEKTHLFDFAIERAKLTGLELGRVGASVAAFNNLYLPHLH
RAGYVAPSKPASDGIESPGGYVMDSVPGLYQHILVLDFKSLYPSIIRTFLIDPKGLVEGLTQVEEQTVSGFLGAQFSREK
PILPMLIKNLSEQRELAKAHHDAPLSQAIKIIMNSLYGVLGSRGCVFHDARLASSITMRGHEIMKQTRLWIEAEGYKVIY
GDTDSTFVWLGNDFEQEQVAQTGKSLAEKVNQLWRDKLTQEFKLESFLELEFETHFEQFFMPTLRGSVEGSKKRYVGTKR
QTSGELGLVFKGMEQVRSDWSPLARRVQYELYERLFQHLDVTTYLREIIDSLHSGELDDELIFTKHLRRNLEDYTAKSAP
HLKVARALCDINQDQRFAKRGARIEYVMTLNGAEPAEQTSNLIDYDYYLSRQLEPIAEPILSILKTSFSEVATNQMSLI
>Mature_799_residues
MNSRLNEQQVIKGRVLTRHAVNRQGGLHLQYFVQTENGPVTVEIPNQEHVCFVQATDATALLASSELRGVRRSPVALSCF
ANTPVEALYCVDGRQQRNLNRAAKDLDIELFESDIKVEHRFLVERFIALDAEFYGHFVARTGTNAVSQQQPLFIASRARQ
AVSTIALNAISLDFECSFAGELYSVGLYGQTTEGCVYQKVIMVGEAEETDANYIEWVENEAQLINQLISWFAEYDPDIII
GWSVVTFDLALLYRRAKHHGIALTIGRDNHPLGWKVEDKYRPETLSLPGRVVLDGIDWLKAAFYQFDRYSLEFVSQALLD
EGKAIHNVDNRMDEINDLFLNNKPALAHYNLTDCRLVWDIFEKTHLFDFAIERAKLTGLELGRVGASVAAFNNLYLPHLH
RAGYVAPSKPASDGIESPGGYVMDSVPGLYQHILVLDFKSLYPSIIRTFLIDPKGLVEGLTQVEEQTVSGFLGAQFSREK
PILPMLIKNLSEQRELAKAHHDAPLSQAIKIIMNSLYGVLGSRGCVFHDARLASSITMRGHEIMKQTRLWIEAEGYKVIY
GDTDSTFVWLGNDFEQEQVAQTGKSLAEKVNQLWRDKLTQEFKLESFLELEFETHFEQFFMPTLRGSVEGSKKRYVGTKR
QTSGELGLVFKGMEQVRSDWSPLARRVQYELYERLFQHLDVTTYLREIIDSLHSGELDDELIFTKHLRRNLEDYTAKSAP
HLKVARALCDINQDQRFAKRGARIEYVMTLNGAEPAEQTSNLIDYDYYLSRQLEPIAEPILSILKTSFSEVATNQMSLI

Specific function: Thought to be involved in DNA repair and/or mutagenesis [H]

COG id: COG0417

COG function: function code L; DNA polymerase elongation subunit (family B)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA polymerase type-B family [H]

Homologues:

Organism=Homo sapiens, GI156616275, Length=649, Percent_Identity=27.1186440677966, Blast_Score=141, Evalue=2e-33,
Organism=Homo sapiens, GI106507301, Length=649, Percent_Identity=25.8859784283513, Blast_Score=123, Evalue=6e-28,
Organism=Homo sapiens, GI153792012, Length=661, Percent_Identity=23.6006051437216, Blast_Score=85, Evalue=3e-16,
Organism=Escherichia coli, GI1786246, Length=801, Percent_Identity=45.9425717852684, Blast_Score=673, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17559488, Length=718, Percent_Identity=27.2980501392758, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI32565317, Length=649, Percent_Identity=25.8859784283513, Blast_Score=125, Evalue=8e-29,
Organism=Saccharomyces cerevisiae, GI6320101, Length=648, Percent_Identity=25.6172839506173, Blast_Score=113, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6324227, Length=494, Percent_Identity=24.4939271255061, Blast_Score=100, Evalue=7e-22,
Organism=Saccharomyces cerevisiae, GI6325090, Length=663, Percent_Identity=22.6244343891403, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24664937, Length=792, Percent_Identity=26.3888888888889, Blast_Score=125, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24648780, Length=415, Percent_Identity=27.9518072289157, Blast_Score=118, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006172
- InterPro:   IPR017964
- InterPro:   IPR006133
- InterPro:   IPR006134
- InterPro:   IPR023211
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00136 DNA_pol_B; PF03104 DNA_pol_B_exo [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 90748; Mature: 90748

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: PS00116 DNA_POLYMERASE_B

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSRLNEQQVIKGRVLTRHAVNRQGGLHLQYFVQTENGPVTVEIPNQEHVCFVQATDATA
CCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCEEEEECCCCCEEEEEECCCHH
LLASSELRGVRRSPVALSCFANTPVEALYCVDGRQQRNLNRAAKDLDIELFESDIKVEHR
HHHHHHHCCCCCCCEEEEEECCCCCEEEEECCCHHHHHHHHHHHHCCHHHHHHHHHHHHH
FLVERFIALDAEFYGHFVARTGTNAVSQQQPLFIASRARQAVSTIALNAISLDFECSFAG
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHEEEEEEEEEECC
ELYSVGLYGQTTEGCVYQKVIMVGEAEETDANYIEWVENEAQLINQLISWFAEYDPDIII
CEEEEECCCCCCCCHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
GWSVVTFDLALLYRRAKHHGIALTIGRDNHPLGWKVEDKYRPETLSLPGRVVLDGIDWLK
EHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCEECCCCCCCEECCCCEEEECHHHHHH
AAFYQFDRYSLEFVSQALLDEGKAIHNVDNRMDEINDLFLNNKPALAHYNLTDCRLVWDI
HHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHH
FEKTHLFDFAIERAKLTGLELGRVGASVAAFNNLYLPHLHRAGYVAPSKPASDGIESPGG
HHHHHHHHHHHHHHHHCCCHHHHHCHHHHHHHCCCCCCHHHCCCCCCCCCCCCCCCCCCC
YVMDSVPGLYQHILVLDFKSLYPSIIRTFLIDPKGLVEGLTQVEEQTVSGFLGAQFSREK
EECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
PILPMLIKNLSEQRELAKAHHDAPLSQAIKIIMNSLYGVLGSRGCVFHDARLASSITMRG
CHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHH
HEIMKQTRLWIEAEGYKVIYGDTDSTFVWLGNDFEQEQVAQTGKSLAEKVNQLWRDKLTQ
HHHHHHHHEEEEECCEEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
EFKLESFLELEFETHFEQFFMPTLRGSVEGSKKRYVGTKRQTSGELGLVFKGMEQVRSDW
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCCCCCCCCCCHHHHHHHHHHHCCH
SPLARRVQYELYERLFQHLDVTTYLREIIDSLHSGELDDELIFTKHLRRNLEDYTAKSAP
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHCCCCC
HLKVARALCDINQDQRFAKRGARIEYVMTLNGAEPAEQTSNLIDYDYYLSRQLEPIAEPI
HHHHHHHHHCCCCHHHHHHCCCEEEEEEEECCCCCHHHHCCCCCHHHHHHHCCHHHHHHH
LSILKTSFSEVATNQMSLI
HHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNSRLNEQQVIKGRVLTRHAVNRQGGLHLQYFVQTENGPVTVEIPNQEHVCFVQATDATA
CCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCEEEEECCCCCEEEEEECCCHH
LLASSELRGVRRSPVALSCFANTPVEALYCVDGRQQRNLNRAAKDLDIELFESDIKVEHR
HHHHHHHCCCCCCCEEEEEECCCCCEEEEECCCHHHHHHHHHHHHCCHHHHHHHHHHHHH
FLVERFIALDAEFYGHFVARTGTNAVSQQQPLFIASRARQAVSTIALNAISLDFECSFAG
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHEEEEEEEEEECC
ELYSVGLYGQTTEGCVYQKVIMVGEAEETDANYIEWVENEAQLINQLISWFAEYDPDIII
CEEEEECCCCCCCCHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
GWSVVTFDLALLYRRAKHHGIALTIGRDNHPLGWKVEDKYRPETLSLPGRVVLDGIDWLK
EHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCEECCCCCCCEECCCCEEEECHHHHHH
AAFYQFDRYSLEFVSQALLDEGKAIHNVDNRMDEINDLFLNNKPALAHYNLTDCRLVWDI
HHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHH
FEKTHLFDFAIERAKLTGLELGRVGASVAAFNNLYLPHLHRAGYVAPSKPASDGIESPGG
HHHHHHHHHHHHHHHHCCCHHHHHCHHHHHHHCCCCCCHHHCCCCCCCCCCCCCCCCCCC
YVMDSVPGLYQHILVLDFKSLYPSIIRTFLIDPKGLVEGLTQVEEQTVSGFLGAQFSREK
EECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
PILPMLIKNLSEQRELAKAHHDAPLSQAIKIIMNSLYGVLGSRGCVFHDARLASSITMRG
CHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHH
HEIMKQTRLWIEAEGYKVIYGDTDSTFVWLGNDFEQEQVAQTGKSLAEKVNQLWRDKLTQ
HHHHHHHHEEEEECCEEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
EFKLESFLELEFETHFEQFFMPTLRGSVEGSKKRYVGTKRQTSGELGLVFKGMEQVRSDW
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCCCCCCCCCCHHHHHHHHHHHCCH
SPLARRVQYELYERLFQHLDVTTYLREIIDSLHSGELDDELIFTKHLRRNLEDYTAKSAP
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHCCCCC
HLKVARALCDINQDQRFAKRGARIEYVMTLNGAEPAEQTSNLIDYDYYLSRQLEPIAEPI
HHHHHHHHHCCCCHHHHHHCCCEEEEEEEECCCCCHHHHCCCCCHHHHHHHCCHHHHHHH
LSILKTSFSEVATNQMSLI
HHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2034216; 2261080; 1630901; 9278503; 2217198; 9079692 [H]