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The map label for this gene is fadI [H]

Identifier: 157962420

GI number: 157962420

Start: 3165668

End: 3166978

Strand: Reverse

Name: fadI [H]

Synonym: Spea_2599

Alternate gene names: 157962420

Gene position: 3166978-3165668 (Counterclockwise)

Preceding gene: 157962436

Following gene: 157962419

Centisome position: 61.2

GC content: 49.28

Gene sequence:

>1311_bases
ATGAGTGATAGACAACAGGTGACGAATGCGAGGGGCGAGCGGATCGCCATTGTTTCGGGATTACGTACACCATTTGCTAA
ACAAGCCACCGCTTTTCATGGTGTGTCGGCATTAGATATGGGCAAGATGGTTGTAAATGAACTGCTTGCTCGCTCAGAGT
TGGATCCTAAAGAGATTGAGCAACTTGTTTATGGGCAAGTGGTACAGATGCCTGCTGCGCCAAACATCGCTCGGGAAATC
GTACTGGGTACGGGCATGAATGTTAGTACCGACGCTTACAGCGTAACCCGTGCTTGTGCAACCAGCTTTCAGTCTACGGT
CAACGTTGCTGAGTCTATCATGACGGGGAATCTCGATATCGGTATCGCGGGCGGTGCAGATTCATCGTCAGTCTTACCAA
TCGGTGTTTCTAAAAAACTAGCTCATGCCTTGGTCGATCTTAATAAAGCCCGCAGTCTTGGGCAGAGACTTTCGATTTTT
CGTCGCTTAGGCCTTAAAGACTTATTACCTGTTCCACCTGCCGTTGCCGAATACTCAACTGGTTTATCTATGGGGCAAAC
AGCTGAGCAGATGGCCAAGACTTATAACATTAGCCGCGCCGATCAAGATGCGTTAGCGCATCGCTCACACACCTTAGCGA
CTGAAACTTGGAATGCAGGCAAGCTGGCCGAAGAGGTGATGGTTGCCCATGTGCCGCCTTATAAAGCCTTTATTGATCGC
GATAACAACATTCGTGAAAACTCTAAGCTTGAGTCTTACGCAAAATTGCGTCCCGCCTTTGATCGTAAGCACGGCTCGGT
AACGGCTGCAAACAGTACGCCATTAACAGATGGTGCATCGGCCGTACTCTTGATGAGTGAAGGTCGTGCAAAAGCATTGG
GTTACACACCTATTGGTTACATTAAGAGTTACGCTTTCACCGCCATCGATGTTTGGGAAGACATGTTGATGGGGCCATCT
TACGCCACGCCTTTGGCGCTTAAGCGTGCAGGGATGGAGCTTGAGGACTTAACCTTAATTGAGATGCATGAAGCCTTTGC
TGCGCAAACCTTGGCTAACATGCAAATGTTTGGCTCGAAAAAATTTGCCGAAGAGAAACTTGGTCGTAATCGAGCTATAG
GTGAAATCGATATGAGTAAGTTTAATGTGCTTGGTGGCTCTTTGGCCTACGGTCACCCATTTGCTGCGACGGGCACGCGC
TTAGTGACTCAGGTGTGTCATGAACTCAAGCGCCGCGGTGGTGGTACGGGTCTCGCAACAGCATGTGCTGCGGGTGGGTT
AGGTGCAGCAATGATTGTGGAAGTGGAGTAA

Upstream 100 bases:

>100_bases
ACACTTTAATGGTCAGACCTGTCTGGTTCAAAGATAAATAGGTTTCCCAAGGATATAAGAAAGCGTGCAACTTCAGGCGT
GCAGAATAAGAGGGCGAAAA

Downstream 100 bases:

>100_bases
CTCTTATGGAAAAGACATTTAATTTAGCTCGTCGTGATGATGGTATTGCCATATTGACTATGGACGTACCAGGCGAAACC
ATGAACACGCTTCGCAGCGA

Product: 3-ketoacyl-CoA thiolase

Products: NA

Alternate protein names: ACSs; Acetyl-CoA acyltransferase; Acyl-CoA ligase; Beta-ketothiolase; Fatty acid oxidation complex subunit beta [H]

Number of amino acids: Translated: 436; Mature: 435

Protein sequence:

>436_residues
MSDRQQVTNARGERIAIVSGLRTPFAKQATAFHGVSALDMGKMVVNELLARSELDPKEIEQLVYGQVVQMPAAPNIAREI
VLGTGMNVSTDAYSVTRACATSFQSTVNVAESIMTGNLDIGIAGGADSSSVLPIGVSKKLAHALVDLNKARSLGQRLSIF
RRLGLKDLLPVPPAVAEYSTGLSMGQTAEQMAKTYNISRADQDALAHRSHTLATETWNAGKLAEEVMVAHVPPYKAFIDR
DNNIRENSKLESYAKLRPAFDRKHGSVTAANSTPLTDGASAVLLMSEGRAKALGYTPIGYIKSYAFTAIDVWEDMLMGPS
YATPLALKRAGMELEDLTLIEMHEAFAAQTLANMQMFGSKKFAEEKLGRNRAIGEIDMSKFNVLGGSLAYGHPFAATGTR
LVTQVCHELKRRGGGTGLATACAAGGLGAAMIVEVE

Sequences:

>Translated_436_residues
MSDRQQVTNARGERIAIVSGLRTPFAKQATAFHGVSALDMGKMVVNELLARSELDPKEIEQLVYGQVVQMPAAPNIAREI
VLGTGMNVSTDAYSVTRACATSFQSTVNVAESIMTGNLDIGIAGGADSSSVLPIGVSKKLAHALVDLNKARSLGQRLSIF
RRLGLKDLLPVPPAVAEYSTGLSMGQTAEQMAKTYNISRADQDALAHRSHTLATETWNAGKLAEEVMVAHVPPYKAFIDR
DNNIRENSKLESYAKLRPAFDRKHGSVTAANSTPLTDGASAVLLMSEGRAKALGYTPIGYIKSYAFTAIDVWEDMLMGPS
YATPLALKRAGMELEDLTLIEMHEAFAAQTLANMQMFGSKKFAEEKLGRNRAIGEIDMSKFNVLGGSLAYGHPFAATGTR
LVTQVCHELKRRGGGTGLATACAAGGLGAAMIVEVE
>Mature_435_residues
SDRQQVTNARGERIAIVSGLRTPFAKQATAFHGVSALDMGKMVVNELLARSELDPKEIEQLVYGQVVQMPAAPNIAREIV
LGTGMNVSTDAYSVTRACATSFQSTVNVAESIMTGNLDIGIAGGADSSSVLPIGVSKKLAHALVDLNKARSLGQRLSIFR
RLGLKDLLPVPPAVAEYSTGLSMGQTAEQMAKTYNISRADQDALAHRSHTLATETWNAGKLAEEVMVAHVPPYKAFIDRD
NNIRENSKLESYAKLRPAFDRKHGSVTAANSTPLTDGASAVLLMSEGRAKALGYTPIGYIKSYAFTAIDVWEDMLMGPSY
ATPLALKRAGMELEDLTLIEMHEAFAAQTLANMQMFGSKKFAEEKLGRNRAIGEIDMSKFNVLGGSLAYGHPFAATGTRL
VTQVCHELKRRGGGTGLATACAAGGLGAAMIVEVE

Specific function: Catalyzes the final step of fatty acid oxidation in which acetyl-CoA is released and the CoA ester of a fatty acid two carbons shorter is formed [H]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI4504327, Length=420, Percent_Identity=41.6666666666667, Blast_Score=338, Evalue=4e-93,
Organism=Homo sapiens, GI4557237, Length=434, Percent_Identity=29.0322580645161, Blast_Score=196, Evalue=3e-50,
Organism=Homo sapiens, GI167614485, Length=423, Percent_Identity=30.9692671394799, Blast_Score=189, Evalue=4e-48,
Organism=Homo sapiens, GI148539872, Length=431, Percent_Identity=30.1624129930394, Blast_Score=186, Evalue=5e-47,
Organism=Homo sapiens, GI4501853, Length=430, Percent_Identity=29.7674418604651, Blast_Score=177, Evalue=2e-44,
Organism=Escherichia coli, GI1788683, Length=426, Percent_Identity=74.1784037558685, Blast_Score=647, Evalue=0.0,
Organism=Escherichia coli, GI87082165, Length=426, Percent_Identity=33.3333333333333, Blast_Score=208, Evalue=6e-55,
Organism=Escherichia coli, GI1788554, Length=423, Percent_Identity=35.2245862884161, Blast_Score=198, Evalue=5e-52,
Organism=Escherichia coli, GI48994986, Length=433, Percent_Identity=32.7944572748268, Blast_Score=182, Evalue=3e-47,
Organism=Escherichia coli, GI1787663, Length=424, Percent_Identity=32.0754716981132, Blast_Score=177, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI17551802, Length=421, Percent_Identity=40.1425178147268, Blast_Score=306, Evalue=2e-83,
Organism=Caenorhabditis elegans, GI133906874, Length=426, Percent_Identity=31.4553990610329, Blast_Score=191, Evalue=7e-49,
Organism=Caenorhabditis elegans, GI25147385, Length=421, Percent_Identity=27.790973871734, Blast_Score=166, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI17535921, Length=428, Percent_Identity=28.5046728971963, Blast_Score=158, Evalue=4e-39,
Organism=Caenorhabditis elegans, GI17535917, Length=431, Percent_Identity=22.969837587007, Blast_Score=102, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6325229, Length=430, Percent_Identity=29.3023255813954, Blast_Score=177, Evalue=3e-45,
Organism=Saccharomyces cerevisiae, GI6322031, Length=442, Percent_Identity=29.4117647058824, Blast_Score=162, Evalue=1e-40,
Organism=Drosophila melanogaster, GI17137578, Length=434, Percent_Identity=41.2442396313364, Blast_Score=330, Evalue=1e-90,
Organism=Drosophila melanogaster, GI24655093, Length=424, Percent_Identity=30.4245283018868, Blast_Score=186, Evalue=2e-47,
Organism=Drosophila melanogaster, GI17648125, Length=429, Percent_Identity=29.1375291375291, Blast_Score=165, Evalue=5e-41,
Organism=Drosophila melanogaster, GI24640423, Length=427, Percent_Identity=26.6978922716628, Blast_Score=154, Evalue=9e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012806
- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.16 [H]

Molecular weight: Translated: 46595; Mature: 46464

Theoretical pI: Translated: 8.09; Mature: 8.09

Prosite motif: PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDRQQVTNARGERIAIVSGLRTPFAKQATAFHGVSALDMGKMVVNELLARSELDPKEIE
CCCHHHHHHCCCCEEEEEECCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHH
QLVYGQVVQMPAAPNIAREIVLGTGMNVSTDAYSVTRACATSFQSTVNVAESIMTGNLDI
HHHHHHHHCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
GIAGGADSSSVLPIGVSKKLAHALVDLNKARSLGQRLSIFRRLGLKDLLPVPPAVAEYST
EEECCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCHHHHHHC
GLSMGQTAEQMAKTYNISRADQDALAHRSHTLATETWNAGKLAEEVMVAHVPPYKAFIDR
CCCCCHHHHHHHHHHCCCCCCHHHHHHHCCCCEECCCCCHHHHHHHHHHCCCCHHHHHCC
DNNIRENSKLESYAKLRPAFDRKHGSVTAANSTPLTDGASAVLLMSEGRAKALGYTPIGY
CCCCCCHHHHHHHHHHCHHHHCCCCCEEECCCCCCCCCCCEEEEEECCCCCCCCCCCHHH
IKSYAFTAIDVWEDMLMGPSYATPLALKRAGMELEDLTLIEMHEAFAAQTLANMQMFGSK
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCH
KFAEEKLGRNRAIGEIDMSKFNVLGGSLAYGHPFAATGTRLVTQVCHELKRRGGGTGLAT
HHHHHHHCCCCCCCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHH
ACAAGGLGAAMIVEVE
HHHCCCCCEEEEEEEC
>Mature Secondary Structure 
SDRQQVTNARGERIAIVSGLRTPFAKQATAFHGVSALDMGKMVVNELLARSELDPKEIE
CCHHHHHHCCCCEEEEEECCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHH
QLVYGQVVQMPAAPNIAREIVLGTGMNVSTDAYSVTRACATSFQSTVNVAESIMTGNLDI
HHHHHHHHCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
GIAGGADSSSVLPIGVSKKLAHALVDLNKARSLGQRLSIFRRLGLKDLLPVPPAVAEYST
EEECCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCHHHHHHC
GLSMGQTAEQMAKTYNISRADQDALAHRSHTLATETWNAGKLAEEVMVAHVPPYKAFIDR
CCCCCHHHHHHHHHHCCCCCCHHHHHHHCCCCEECCCCCHHHHHHHHHHCCCCHHHHHCC
DNNIRENSKLESYAKLRPAFDRKHGSVTAANSTPLTDGASAVLLMSEGRAKALGYTPIGY
CCCCCCHHHHHHHHHHCHHHHCCCCCEEECCCCCCCCCCCEEEEEECCCCCCCCCCCHHH
IKSYAFTAIDVWEDMLMGPSYATPLALKRAGMELEDLTLIEMHEAFAAQTLANMQMFGSK
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCH
KFAEEKLGRNRAIGEIDMSKFNVLGGSLAYGHPFAATGTRLVTQVCHELKRRGGGTGLAT
HHHHHHHCCCCCCCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHH
ACAAGGLGAAMIVEVE
HHHCCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA