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The map label for this gene is pleD [H]

Identifier: 157962401

GI number: 157962401

Start: 3140202

End: 3142163

Strand: Reverse

Name: pleD [H]

Synonym: Spea_2580

Alternate gene names: 157962401

Gene position: 3142163-3140202 (Counterclockwise)

Preceding gene: 157962405

Following gene: 157962400

Centisome position: 60.72

GC content: 41.34

Gene sequence:

>1962_bases
ATGGAAAAGATCATTGTATTTGTTATTTTATCGAGCATCATGCTGTGCTCGTTTTCAGTTCGAGCCGCAGAATTCTCCCC
AATCAACACCATCGAGACATTTGACAATGGTCAGCAAATTATGGCCTTGTTTACTGATAAAGAACTAGATGGTGAGTCGA
AGCGTCTGCAATTAAATCAGCTGATTTCACAAGCTCAAGTTAATCAGTCTGCTTATTATGCATTTGCTTACTACCGGCTC
TTGCATGACATTGAGTTGTCTGTTTCCGCGCCAGAGGCCGCAGAGTTGGCTATAGCAAGAATGCTGTTATTAGGGCAAAG
ACTGAATTCAGGCTGGTTGATCGCCGAAGCTAAGATGTGGCAGGCCACTTTTGCCGCTAAACAGAGCCGCTTCGATGATG
GGTTAGTATTGATCGATGAGGCTATTCGATTGTCAAAAGTAGTTAATTTTTCCCATCTGACTGGGCGAGCATATAATGTC
AAAGCTGCGCTTTATTTTTTCCAGGATCAGTACTACATCGCGCTTGAATATTATTTGAGAGCTCTGGATATCTTTACAGC
AACTCCAGAGGACCCCTATGTCAGTAAGGTATTGTCTAATGTTGCTATTATCTATGTTGATTTAGAAGATTGGGAAAAGG
CCCTAGAGGCAAATAATAATGCTCTGGCACATGTTGATAAATATGGTGGTAGTCACGAGCAGTTAGCAGCATTCAATAAT
AATGCTTCCTTTATTTTAGGAAAACTTGGACGCTGGAGTGAGAGTAAGCCCTACTTAATTTCCTCACGAGAAAGTGCACT
GTTAAGTGGTAATTTGCGAGTCAAATTAAATGCTCAGACCTCATGGATTCAATATTACCTCGCTCAAGGTGAATATCGTT
TAGCCGTTGAAGAGGGGCTGGAATGTGTGGACATTGCTTTGGATAATCAATACCCGTTGTTCGAAGGGGATTGTAGTCGA
ATATTAGCGAAAGCCATGATCAAGCTTGGGCAAGTCGATGAAGCCCTGCAATTTTTAGCCCATAGTGAATCATTGTATAA
ACGAATAAGAAAGCGCTCAGGGTTAGCCGATACCTTTAATACTTATGCACTAGCCTATGAGGTAAAGGGGAATTATAAAG
CCGCATTAGACTATCAGCGAAAGGCTAATGAAGAAGATAAGGCACTCTTGTTTGATCGACGAGCCAAGATGACATTTGAC
CTGGGTCAACGTTATCAAGATAAGTACCGTCAACAAGAATTAGCCGTTTTAAAAGCCGAAAATGATTTACAAGAGTCTCG
ATTCGCAGAGCAACAGCTACGGGAGAAGTTCCTATTCTTTTTTATTCTTGTTTCTGTTATTTCGATCTTTCTGCTGATAA
AGAAACGCTTCGGTTTAGAAAATGATAATAAGAATCTGCAATCCTCTAACCTTGAGTTGTATAAACAGTCACATATCGAC
GCGCTAACAGGTTTATATAATCGTCGTTATTTCTCTGATTTTTTGGAGCAACAGCATCAACTTTCGCAGAGTCAAAGCCT
GATGATGAGTTTAGCCATTATCGATATAGACCATTTTAAGAGCGTGAATGACAGCTATGGTCATGATGTCGGTGATGAGG
TGCTTATTCAGGTCGGTAATGTGATAAATAAGAATATTCGTGAGCGTGATTTAATTATTCGATGGGGTGGAGAGGAGTTT
GTCCTGCTCTTGAGTTGGCCAATATCTTCATATCCACAGACAGGAGTTGACTTGCTTGAACATTTTGAACGTGTTCGTCA
GGCTATTGAGAATACAGTTATTGTTGTTGGTGAGTACAAATTGAAAGTAACTATCTCAATGGGAGTGGCGCAACCTCTAG
GTGCGCTGGATCTGATTGAATCATGGCATCATGTACTTGATGGCGCAGACAAAGCGCTTTATCGAGCCAAGAGACAAGGA
CGAAATTGTGTGGTGATTGCTAAGAAGGGCTCGCCGCTATAG

Upstream 100 bases:

>100_bases
TGACAAAGACGTTGAATATAAGGTTATGTTTTATAAGCTTATCTTAGGACTTACTTAATCAGGGTGAACAGCTTATAAAA
CCACAATTAGCGATATTCTT

Downstream 100 bases:

>100_bases
GGAAGTGGTTGAAGGTAAACAATAAAGTAATAGTGCTTCAAGCTGCTTACAGACAGAGTTGGTATTGACCAGCTTTTATT
CAGCTCTTAGTCTGTAAGGT

Product: diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 653; Mature: 653

Protein sequence:

>653_residues
MEKIIVFVILSSIMLCSFSVRAAEFSPINTIETFDNGQQIMALFTDKELDGESKRLQLNQLISQAQVNQSAYYAFAYYRL
LHDIELSVSAPEAAELAIARMLLLGQRLNSGWLIAEAKMWQATFAAKQSRFDDGLVLIDEAIRLSKVVNFSHLTGRAYNV
KAALYFFQDQYYIALEYYLRALDIFTATPEDPYVSKVLSNVAIIYVDLEDWEKALEANNNALAHVDKYGGSHEQLAAFNN
NASFILGKLGRWSESKPYLISSRESALLSGNLRVKLNAQTSWIQYYLAQGEYRLAVEEGLECVDIALDNQYPLFEGDCSR
ILAKAMIKLGQVDEALQFLAHSESLYKRIRKRSGLADTFNTYALAYEVKGNYKAALDYQRKANEEDKALLFDRRAKMTFD
LGQRYQDKYRQQELAVLKAENDLQESRFAEQQLREKFLFFFILVSVISIFLLIKKRFGLENDNKNLQSSNLELYKQSHID
ALTGLYNRRYFSDFLEQQHQLSQSQSLMMSLAIIDIDHFKSVNDSYGHDVGDEVLIQVGNVINKNIRERDLIIRWGGEEF
VLLLSWPISSYPQTGVDLLEHFERVRQAIENTVIVVGEYKLKVTISMGVAQPLGALDLIESWHHVLDGADKALYRAKRQG
RNCVVIAKKGSPL

Sequences:

>Translated_653_residues
MEKIIVFVILSSIMLCSFSVRAAEFSPINTIETFDNGQQIMALFTDKELDGESKRLQLNQLISQAQVNQSAYYAFAYYRL
LHDIELSVSAPEAAELAIARMLLLGQRLNSGWLIAEAKMWQATFAAKQSRFDDGLVLIDEAIRLSKVVNFSHLTGRAYNV
KAALYFFQDQYYIALEYYLRALDIFTATPEDPYVSKVLSNVAIIYVDLEDWEKALEANNNALAHVDKYGGSHEQLAAFNN
NASFILGKLGRWSESKPYLISSRESALLSGNLRVKLNAQTSWIQYYLAQGEYRLAVEEGLECVDIALDNQYPLFEGDCSR
ILAKAMIKLGQVDEALQFLAHSESLYKRIRKRSGLADTFNTYALAYEVKGNYKAALDYQRKANEEDKALLFDRRAKMTFD
LGQRYQDKYRQQELAVLKAENDLQESRFAEQQLREKFLFFFILVSVISIFLLIKKRFGLENDNKNLQSSNLELYKQSHID
ALTGLYNRRYFSDFLEQQHQLSQSQSLMMSLAIIDIDHFKSVNDSYGHDVGDEVLIQVGNVINKNIRERDLIIRWGGEEF
VLLLSWPISSYPQTGVDLLEHFERVRQAIENTVIVVGEYKLKVTISMGVAQPLGALDLIESWHHVLDGADKALYRAKRQG
RNCVVIAKKGSPL
>Mature_653_residues
MEKIIVFVILSSIMLCSFSVRAAEFSPINTIETFDNGQQIMALFTDKELDGESKRLQLNQLISQAQVNQSAYYAFAYYRL
LHDIELSVSAPEAAELAIARMLLLGQRLNSGWLIAEAKMWQATFAAKQSRFDDGLVLIDEAIRLSKVVNFSHLTGRAYNV
KAALYFFQDQYYIALEYYLRALDIFTATPEDPYVSKVLSNVAIIYVDLEDWEKALEANNNALAHVDKYGGSHEQLAAFNN
NASFILGKLGRWSESKPYLISSRESALLSGNLRVKLNAQTSWIQYYLAQGEYRLAVEEGLECVDIALDNQYPLFEGDCSR
ILAKAMIKLGQVDEALQFLAHSESLYKRIRKRSGLADTFNTYALAYEVKGNYKAALDYQRKANEEDKALLFDRRAKMTFD
LGQRYQDKYRQQELAVLKAENDLQESRFAEQQLREKFLFFFILVSVISIFLLIKKRFGLENDNKNLQSSNLELYKQSHID
ALTGLYNRRYFSDFLEQQHQLSQSQSLMMSLAIIDIDHFKSVNDSYGHDVGDEVLIQVGNVINKNIRERDLIIRWGGEEF
VLLLSWPISSYPQTGVDLLEHFERVRQAIENTVIVVGEYKLKVTISMGVAQPLGALDLIESWHHVLDGADKALYRAKRQG
RNCVVIAKKGSPL

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI87081881, Length=179, Percent_Identity=40.7821229050279, Blast_Score=105, Evalue=7e-24,
Organism=Escherichia coli, GI1787262, Length=173, Percent_Identity=42.1965317919075, Blast_Score=105, Evalue=1e-23,
Organism=Escherichia coli, GI1786584, Length=210, Percent_Identity=31.4285714285714, Blast_Score=101, Evalue=2e-22,
Organism=Escherichia coli, GI145693134, Length=172, Percent_Identity=33.7209302325581, Blast_Score=99, Evalue=1e-21,
Organism=Escherichia coli, GI1787816, Length=242, Percent_Identity=29.3388429752066, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI1787802, Length=168, Percent_Identity=34.5238095238095, Blast_Score=90, Evalue=5e-19,
Organism=Escherichia coli, GI87082007, Length=180, Percent_Identity=33.3333333333333, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI87081974, Length=155, Percent_Identity=36.1290322580645, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI87081977, Length=190, Percent_Identity=31.0526315789474, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1788085, Length=169, Percent_Identity=33.7278106508876, Blast_Score=79, Evalue=8e-16,
Organism=Escherichia coli, GI1788381, Length=180, Percent_Identity=33.8888888888889, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1787541, Length=173, Percent_Identity=28.9017341040462, Blast_Score=67, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 74556; Mature: 74556

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: PS50293 TPR_REGION ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKIIVFVILSSIMLCSFSVRAAEFSPINTIETFDNGQQIMALFTDKELDGESKRLQLNQ
CCCEEHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCCEEEEEEECCCCCCCHHHHHHHH
LISQAQVNQSAYYAFAYYRLLHDIELSVSAPEAAELAIARMLLLGQRLNSGWLIAEAKMW
HHHHHHCCCHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHHHHCCCCCEEEEEHHHH
QATFAAKQSRFDDGLVLIDEAIRLSKVVNFSHLTGRAYNVKAALYFFQDQYYIALEYYLR
HHHHHHHHCCCCCCEEEHHHHHHHHHHHCHHHHCCCEEEEEEEEEEEECCEEEHHHHHHH
ALDIFTATPEDPYVSKVLSNVAIIYVDLEDWEKALEANNNALAHVDKYGGSHEQLAAFNN
HHHHHCCCCCCHHHHHHHCCEEEEEEEHHHHHHHHHCCCCCEEEEHHCCCCHHHHEEECC
NASFILGKLGRWSESKPYLISSRESALLSGNLRVKLNAQTSWIQYYLAQGEYRLAVEEGL
CCCEEEECCCCCCCCCCEEEECCCCEEEECCEEEEEECCHHHHHHHHHCCCEEEEHHCCC
ECVDIALDNQYPLFEGDCSRILAKAMIKLGQVDEALQFLAHSESLYKRIRKRSGLADTFN
EEEEEEECCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHCCCCCCCC
TYALAYEVKGNYKAALDYQRKANEEDKALLFDRRAKMTFDLGQRYQDKYRQQELAVLKAE
CEEEEEEECCCEEEEECHHHHCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHEECC
NDLQESRFAEQQLREKFLFFFILVSVISIFLLIKKRFGLENDNKNLQSSNLELYKQSHID
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHH
ALTGLYNRRYFSDFLEQQHQLSQSQSLMMSLAIIDIDHFKSVNDSYGHDVGDEVLIQVGN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEHHHHCCCCCCCCCCCCHHHHHHHHH
VINKNIRERDLIIRWGGEEFVLLLSWPISSYPQTGVDLLEHFERVRQAIENTVIVVGEYK
HHCCCCCCCEEEEEECCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEE
LKVTISMGVAQPLGALDLIESWHHVLDGADKALYRAKRQGRNCVVIAKKGSPL
EEEEEEECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCCEEEEECCCCCC
>Mature Secondary Structure
MEKIIVFVILSSIMLCSFSVRAAEFSPINTIETFDNGQQIMALFTDKELDGESKRLQLNQ
CCCEEHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCCEEEEEEECCCCCCCHHHHHHHH
LISQAQVNQSAYYAFAYYRLLHDIELSVSAPEAAELAIARMLLLGQRLNSGWLIAEAKMW
HHHHHHCCCHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHHHHCCCCCEEEEEHHHH
QATFAAKQSRFDDGLVLIDEAIRLSKVVNFSHLTGRAYNVKAALYFFQDQYYIALEYYLR
HHHHHHHHCCCCCCEEEHHHHHHHHHHHCHHHHCCCEEEEEEEEEEEECCEEEHHHHHHH
ALDIFTATPEDPYVSKVLSNVAIIYVDLEDWEKALEANNNALAHVDKYGGSHEQLAAFNN
HHHHHCCCCCCHHHHHHHCCEEEEEEEHHHHHHHHHCCCCCEEEEHHCCCCHHHHEEECC
NASFILGKLGRWSESKPYLISSRESALLSGNLRVKLNAQTSWIQYYLAQGEYRLAVEEGL
CCCEEEECCCCCCCCCCEEEECCCCEEEECCEEEEEECCHHHHHHHHHCCCEEEEHHCCC
ECVDIALDNQYPLFEGDCSRILAKAMIKLGQVDEALQFLAHSESLYKRIRKRSGLADTFN
EEEEEEECCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHCCCCCCCC
TYALAYEVKGNYKAALDYQRKANEEDKALLFDRRAKMTFDLGQRYQDKYRQQELAVLKAE
CEEEEEEECCCEEEEECHHHHCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHEECC
NDLQESRFAEQQLREKFLFFFILVSVISIFLLIKKRFGLENDNKNLQSSNLELYKQSHID
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHH
ALTGLYNRRYFSDFLEQQHQLSQSQSLMMSLAIIDIDHFKSVNDSYGHDVGDEVLIQVGN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEHHHHCCCCCCCCCCCCHHHHHHHHH
VINKNIRERDLIIRWGGEEFVLLLSWPISSYPQTGVDLLEHFERVRQAIENTVIVVGEYK
HHCCCCCCCEEEEEECCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEE
LKVTISMGVAQPLGALDLIESWHHVLDGADKALYRAKRQGRNCVVIAKKGSPL
EEEEEEECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA