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The map label for this gene is ppsA [H]

Identifier: 157962370

GI number: 157962370

Start: 3101185

End: 3103554

Strand: Reverse

Name: ppsA [H]

Synonym: Spea_2549

Alternate gene names: 157962370

Gene position: 3103554-3101185 (Counterclockwise)

Preceding gene: 157962373

Following gene: 157962369

Centisome position: 59.98

GC content: 45.27

Gene sequence:

>2370_bases
GTGCAGCAATACGTACTCTGGTATCAAGAATTAGGCATGGGCGATGTTAACAAGGTAGGCGGTAAAAACGCTTCTCTTGG
TGAGATGATTAGCAACCTATCAAACGCAGGCGTTCAAGTACCTGGTGGATTTGCGACAACATCTTTTGCGTTCAATGAAT
TTCTTGAACAAAGTGGACTTAACCAGAAGATATACGAAATTCTAGAAACGCTAGATGTAGATGACGTGAAAGCACTCGCT
ACAGCCGGTGCACAGATTAGACAGTGGGTTATCGACACCCCATTCCACCCAGAATTTGAGCAAGCGGTTCGTGAAGCCTA
CGACAAGCTTAGCTCTGAAACAACTGATGCTTCTTTTGCGGTTCGCTCTTCAGCGACAGCAGAAGATATGCCTGACGCAT
CATTTGCTGGCCAACAAGAAACTTTCCTTAACGTTAAGGGTTATGATGCCGTTTTAGAAGCAACTAAGCATGTATTTGCT
TCTTTGTTTAATGACCGTGCAATTTCATACCGTGTTCACCAAGGATATGACCATAAAGGCGTCGCACTGTCTGCTGGTAT
TCAGCGCATGGTGCGTTCAGACAAAGCTGCTTCTGGTGTTATGTTCACTATGGACACTGAGTCTGGCAACAATGACGTAG
TATTTATTACTTCTTCATTTGGTCTAGGTGAAATGGTTGTTCAAGGCGCGGTTAACCCTGACGAGTTTTATGTTCATAAG
CCAACACTAGTTGCAGGTCATAAAGCGGTTGTACGCCGTAACATCGGTAGCAAGCTTATCCAAATGGTTTATTCAGACGA
TGAATCACACGGCAAGCAAGTTAAGATTGAAGATATTGCAGCAGATAAGCGCCGCGAGTTCTCAATTAACGATGCTGAAG
TACAAGAGCTTGCTAAGCAGGCTATGACCATCGAGAAGCATTACGGTCGTCCAATGGACATCGAATGGGCTAAAGACGGT
AACGACGGTAAGTTGTATATCGTACAGGCTCGTCCTGAAACTGTTCGTTCTCGTGAAGATGTGCAGCTAATCGAACGTTA
CCACCTTAAGACTCAAGGTGATGTTGTTTGTGAGGGTCGTGCAATTGGTCATAAGATTGGTAAAGGTGTCGCTAAGGTAC
TTAAGTCAATCGAAGATATGGATCAAATCCAACCAGGTGACGTGTTAGTTACTGACATGACTGACCCTGATTGGGAGCCA
ATCATGAAGCGCGCAAGCGCTATCGTTACTAACCGTGGTGGCCGTACTTGTCACGCTGCAATTATCGCACGTGAACTTGG
TGTACCAGCAGTTGTTGGTTGTGGCGATGTAACCGAGCGTATCCAAAATGGCCAAGAAGTAACAGTTTCTTGTGCCGAAG
GTGACACAGGCTTTATTTACAATGGTCTACAAGACTTTGAAGTGATTTCATCACGTGTAGATTCAATGCCTGAATTGCCA
ATGAAGATCACTATGAACGTGGGTAACCCAGACAGAGCATTTGACTTTGCTCGTCTTCCTAACGAAGGTGTTGGTCTTGC
GCGTCTTGAGTTCATCATCAATCGCATGATCGGTATTCACCCTAAAGCATTGCTTGAGTTCAACGACCAGACTGCTGAGT
TACAACAAGAGATCCACGAAATGATCGCGGGTTACGAGTCACCAGTAGAATACTACGTGGCACGTCTTGTTGAAGGTATT
GCAACGATTGGTTCAGCTTTCCATCCAAAGAAAGTGATTGTGCGTATGTCTGACTTTAAGTCAAACGAATATGCAAACTT
GGTTGGTGGTGAGCGTTACGAGCCAGAAGAAGAAAACCCAATGCTGGGCTTCCGTGGTGCAAGCCGTTACATCTCAGAAT
CGTTCCGTGATTGTTTTGCGCTAGAGTGTGAAGCGATTAAGCGTGTTCGTAACGATATGGGTCTGAAGAATGTTGAAATC
ATGATCCCATTCGTTCGTACCCTAGACGAAGCGGCGCAAGTGATTGAGCTTCTTAAAGAGCAAGGTCTAGAGCGCGGTAA
AGACGGTCTACGCGTCATTATGATGTGTGAGCTGCCTTCAAATGCACTACTTGCTGAGCAGTTCCTTGAGCACTTCGATG
GTTTCTCTATCGGCTCTAACGATTTAACTCAGTTGACTCTTGGTCTTGATCGAGATTCAGGCATCATCAGTCATCTATTC
GATGAGCGTAATGAAGCGGTTAAAGCGTTACTTGCTCTAGCAATTAAAGCCGCTAAAGCTAAAGGTGCTTACGTTGGTAT
TTGTGGCCAAGGCCCATCAGACCACCCTGACTTTGCAGCTTGGTTGGTTGAGCAAGGCATCGACACTGTATCGCTAAACC
CTGATACAGTTATCGATACTTGGTTATACCTAGCTGAAGCACACGGATAA

Upstream 100 bases:

>100_bases
CAAATAAAATTATACATTTACGCTTGTTGTCATGGAGTGTAAGATGCCTCTGCTCTCGTGTGAAGAGCCACACTAAAATA
TACTTGCGGAGATAAAAACT

Downstream 100 bases:

>100_bases
CAGTCATTGTTAACCGTTAGGATGAAGTGTTAATTCTGTTTGATTTAACATTTTGTTAAAAACATGAAAAAAGCCGCTAT
TTAGCGGCTTTTTTTTGTTT

Product: phosphoenolpyruvate synthase

Products: NA

Alternate protein names: PEP synthase; Pyruvate, water dikinase [H]

Number of amino acids: Translated: 789; Mature: 789

Protein sequence:

>789_residues
MQQYVLWYQELGMGDVNKVGGKNASLGEMISNLSNAGVQVPGGFATTSFAFNEFLEQSGLNQKIYEILETLDVDDVKALA
TAGAQIRQWVIDTPFHPEFEQAVREAYDKLSSETTDASFAVRSSATAEDMPDASFAGQQETFLNVKGYDAVLEATKHVFA
SLFNDRAISYRVHQGYDHKGVALSAGIQRMVRSDKAASGVMFTMDTESGNNDVVFITSSFGLGEMVVQGAVNPDEFYVHK
PTLVAGHKAVVRRNIGSKLIQMVYSDDESHGKQVKIEDIAADKRREFSINDAEVQELAKQAMTIEKHYGRPMDIEWAKDG
NDGKLYIVQARPETVRSREDVQLIERYHLKTQGDVVCEGRAIGHKIGKGVAKVLKSIEDMDQIQPGDVLVTDMTDPDWEP
IMKRASAIVTNRGGRTCHAAIIARELGVPAVVGCGDVTERIQNGQEVTVSCAEGDTGFIYNGLQDFEVISSRVDSMPELP
MKITMNVGNPDRAFDFARLPNEGVGLARLEFIINRMIGIHPKALLEFNDQTAELQQEIHEMIAGYESPVEYYVARLVEGI
ATIGSAFHPKKVIVRMSDFKSNEYANLVGGERYEPEEENPMLGFRGASRYISESFRDCFALECEAIKRVRNDMGLKNVEI
MIPFVRTLDEAAQVIELLKEQGLERGKDGLRVIMMCELPSNALLAEQFLEHFDGFSIGSNDLTQLTLGLDRDSGIISHLF
DERNEAVKALLALAIKAAKAKGAYVGICGQGPSDHPDFAAWLVEQGIDTVSLNPDTVIDTWLYLAEAHG

Sequences:

>Translated_789_residues
MQQYVLWYQELGMGDVNKVGGKNASLGEMISNLSNAGVQVPGGFATTSFAFNEFLEQSGLNQKIYEILETLDVDDVKALA
TAGAQIRQWVIDTPFHPEFEQAVREAYDKLSSETTDASFAVRSSATAEDMPDASFAGQQETFLNVKGYDAVLEATKHVFA
SLFNDRAISYRVHQGYDHKGVALSAGIQRMVRSDKAASGVMFTMDTESGNNDVVFITSSFGLGEMVVQGAVNPDEFYVHK
PTLVAGHKAVVRRNIGSKLIQMVYSDDESHGKQVKIEDIAADKRREFSINDAEVQELAKQAMTIEKHYGRPMDIEWAKDG
NDGKLYIVQARPETVRSREDVQLIERYHLKTQGDVVCEGRAIGHKIGKGVAKVLKSIEDMDQIQPGDVLVTDMTDPDWEP
IMKRASAIVTNRGGRTCHAAIIARELGVPAVVGCGDVTERIQNGQEVTVSCAEGDTGFIYNGLQDFEVISSRVDSMPELP
MKITMNVGNPDRAFDFARLPNEGVGLARLEFIINRMIGIHPKALLEFNDQTAELQQEIHEMIAGYESPVEYYVARLVEGI
ATIGSAFHPKKVIVRMSDFKSNEYANLVGGERYEPEEENPMLGFRGASRYISESFRDCFALECEAIKRVRNDMGLKNVEI
MIPFVRTLDEAAQVIELLKEQGLERGKDGLRVIMMCELPSNALLAEQFLEHFDGFSIGSNDLTQLTLGLDRDSGIISHLF
DERNEAVKALLALAIKAAKAKGAYVGICGQGPSDHPDFAAWLVEQGIDTVSLNPDTVIDTWLYLAEAHG
>Mature_789_residues
MQQYVLWYQELGMGDVNKVGGKNASLGEMISNLSNAGVQVPGGFATTSFAFNEFLEQSGLNQKIYEILETLDVDDVKALA
TAGAQIRQWVIDTPFHPEFEQAVREAYDKLSSETTDASFAVRSSATAEDMPDASFAGQQETFLNVKGYDAVLEATKHVFA
SLFNDRAISYRVHQGYDHKGVALSAGIQRMVRSDKAASGVMFTMDTESGNNDVVFITSSFGLGEMVVQGAVNPDEFYVHK
PTLVAGHKAVVRRNIGSKLIQMVYSDDESHGKQVKIEDIAADKRREFSINDAEVQELAKQAMTIEKHYGRPMDIEWAKDG
NDGKLYIVQARPETVRSREDVQLIERYHLKTQGDVVCEGRAIGHKIGKGVAKVLKSIEDMDQIQPGDVLVTDMTDPDWEP
IMKRASAIVTNRGGRTCHAAIIARELGVPAVVGCGDVTERIQNGQEVTVSCAEGDTGFIYNGLQDFEVISSRVDSMPELP
MKITMNVGNPDRAFDFARLPNEGVGLARLEFIINRMIGIHPKALLEFNDQTAELQQEIHEMIAGYESPVEYYVARLVEGI
ATIGSAFHPKKVIVRMSDFKSNEYANLVGGERYEPEEENPMLGFRGASRYISESFRDCFALECEAIKRVRNDMGLKNVEI
MIPFVRTLDEAAQVIELLKEQGLERGKDGLRVIMMCELPSNALLAEQFLEHFDGFSIGSNDLTQLTLGLDRDSGIISHLF
DERNEAVKALLALAIKAAKAKGAYVGICGQGPSDHPDFAAWLVEQGIDTVSLNPDTVIDTWLYLAEAHG

Specific function: Catalyzes the phosphorylation of pyruvate to phosphoenolpyruvate [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1787994, Length=782, Percent_Identity=75.5754475703325, Blast_Score=1273, Evalue=0.0,
Organism=Escherichia coli, GI1788756, Length=446, Percent_Identity=27.1300448430493, Blast_Score=129, Evalue=9e-31,
Organism=Escherichia coli, GI1788726, Length=190, Percent_Identity=32.6315789473684, Blast_Score=95, Evalue=2e-20,
Organism=Escherichia coli, GI48994992, Length=397, Percent_Identity=25.6926952141058, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1789193, Length=386, Percent_Identity=26.1658031088083, Blast_Score=89, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI17564524, Length=314, Percent_Identity=26.4331210191083, Blast_Score=90, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR006319
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.2 [H]

Molecular weight: Translated: 87095; Mature: 87095

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQYVLWYQELGMGDVNKVGGKNASLGEMISNLSNAGVQVPGGFATTSFAFNEFLEQSGL
CCCCEEEHHHCCCCCCHHCCCCCCCHHHHHHHHHHCCEECCCCCHHHHHHHHHHHHHCCC
NQKIYEILETLDVDDVKALATAGAQIRQWVIDTPFHPEFEQAVREAYDKLSSETTDASFA
CHHHHHHHHHCCHHHHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCEE
VRSSATAEDMPDASFAGQQETFLNVKGYDAVLEATKHVFASLFNDRAISYRVHQGYDHKG
ECCCCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCC
VALSAGIQRMVRSDKAASGVMFTMDTESGNNDVVFITSSFGLGEMVVQGAVNPDEFYVHK
CHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCEEEEECCCCCHHHHHHCCCCCCCEEEEC
PTLVAGHKAVVRRNIGSKLIQMVYSDDESHGKQVKIEDIAADKRREFSINDAEVQELAKQ
CCEECCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEHHHHCCCCCCCCCCHHHHHHHHHH
AMTIEKHYGRPMDIEWAKDGNDGKLYIVQARPETVRSREDVQLIERYHLKTQGDVVCEGR
HHHHHHHCCCCCCEEECCCCCCCEEEEEECCCHHHCCHHHHHHHHHHHCCCCCCEEECCH
AIGHKIGKGVAKVLKSIEDMDQIQPGDVLVTDMTDPDWEPIMKRASAIVTNRGGRTCHAA
HHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCHHHHH
IIARELGVPAVVGCGDVTERIQNGQEVTVSCAEGDTGFIYNGLQDFEVISSRVDSMPELP
HHHHHCCCCEEECCCHHHHHHCCCCEEEEEECCCCCCEEECCHHHHHHHHHHHCCCCCCC
MKITMNVGNPDRAFDFARLPNEGVGLARLEFIINRMIGIHPKALLEFNDQTAELQQEIHE
EEEEEECCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHEECCCHHHHHHHHHHH
MIAGYESPVEYYVARLVEGIATIGSAFHPKKVIVRMSDFKSNEYANLVGGERYEPEEENP
HHCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCHHCCCCCCCCCCCCCC
MLGFRGASRYISESFRDCFALECEAIKRVRNDMGLKNVEIMIPFVRTLDEAAQVIELLKE
CEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHH
QGLERGKDGLRVIMMCELPSNALLAEQFLEHFDGFSIGSNDLTQLTLGLDRDSGIISHLF
CCHHCCCCCCEEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCHHHHHHH
DERNEAVKALLALAIKAAKAKGAYVGICGQGPSDHPDFAAWLVEQGIDTVSLNPDTVIDT
HHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHCCCCEEECCCCHHHHH
WLYLAEAHG
HHHHHHCCC
>Mature Secondary Structure
MQQYVLWYQELGMGDVNKVGGKNASLGEMISNLSNAGVQVPGGFATTSFAFNEFLEQSGL
CCCCEEEHHHCCCCCCHHCCCCCCCHHHHHHHHHHCCEECCCCCHHHHHHHHHHHHHCCC
NQKIYEILETLDVDDVKALATAGAQIRQWVIDTPFHPEFEQAVREAYDKLSSETTDASFA
CHHHHHHHHHCCHHHHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCEE
VRSSATAEDMPDASFAGQQETFLNVKGYDAVLEATKHVFASLFNDRAISYRVHQGYDHKG
ECCCCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCC
VALSAGIQRMVRSDKAASGVMFTMDTESGNNDVVFITSSFGLGEMVVQGAVNPDEFYVHK
CHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCEEEEECCCCCHHHHHHCCCCCCCEEEEC
PTLVAGHKAVVRRNIGSKLIQMVYSDDESHGKQVKIEDIAADKRREFSINDAEVQELAKQ
CCEECCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEHHHHCCCCCCCCCCHHHHHHHHHH
AMTIEKHYGRPMDIEWAKDGNDGKLYIVQARPETVRSREDVQLIERYHLKTQGDVVCEGR
HHHHHHHCCCCCCEEECCCCCCCEEEEEECCCHHHCCHHHHHHHHHHHCCCCCCEEECCH
AIGHKIGKGVAKVLKSIEDMDQIQPGDVLVTDMTDPDWEPIMKRASAIVTNRGGRTCHAA
HHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCHHHHH
IIARELGVPAVVGCGDVTERIQNGQEVTVSCAEGDTGFIYNGLQDFEVISSRVDSMPELP
HHHHHCCCCEEECCCHHHHHHCCCCEEEEEECCCCCCEEECCHHHHHHHHHHHCCCCCCC
MKITMNVGNPDRAFDFARLPNEGVGLARLEFIINRMIGIHPKALLEFNDQTAELQQEIHE
EEEEEECCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHEECCCHHHHHHHHHHH
MIAGYESPVEYYVARLVEGIATIGSAFHPKKVIVRMSDFKSNEYANLVGGERYEPEEENP
HHCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCHHCCCCCCCCCCCCCC
MLGFRGASRYISESFRDCFALECEAIKRVRNDMGLKNVEIMIPFVRTLDEAAQVIELLKE
CEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHH
QGLERGKDGLRVIMMCELPSNALLAEQFLEHFDGFSIGSNDLTQLTLGLDRDSGIISHLF
CCHHCCCCCCEEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCHHHHHHH
DERNEAVKALLALAIKAAKAKGAYVGICGQGPSDHPDFAAWLVEQGIDTVSLNPDTVIDT
HHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHCCCCEEECCCCHHHHH
WLYLAEAHG
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1310524; 9097039; 9278503 [H]