Click here to switch to the map view.

The map label for this gene is ldh [H]

Identifier: 157962364

GI number: 157962364

Start: 3094774

End: 3095805

Strand: Direct

Name: ldh [H]

Synonym: Spea_2543

Alternate gene names: 157962364

Gene position: 3094774-3095805 (Clockwise)

Preceding gene: 157962362

Following gene: 157962365

Centisome position: 59.81

GC content: 45.64

Gene sequence:

>1032_bases
GTGGCAGTTTTTAATCATATCTCTTTCGACGACCATGAACAGGTCGTTTTTTGTCATGATAAAGAAAGCGGCTTAAAAGC
CATTATCGCTATCCACAACACTAATTTAGGCCCTGCAGTTGGCGGTTGTAGAATGTGGAACTATGAGTCTGATGATGAGG
CCATTACCGATGTATTGCGTCTATCTCGTGGCATGACCTATAAAAACGCGCTAGCGGGTTTAGCTATGGGGGGGGGGAAG
TCAGTAATTATCGCCGATCCAAAAGTACAAGATAGAGAAGCACTTTTTAGAGCATTCGGCCGTTGTATCCACACCTTAGG
CGGCAAGTATTACTCAGCTGAAGATGTTGGAGTTTCGACTTCCGACATCATGATTGCCCATCAAGAAACCCCATATATGG
CAGGTTTAGAGGGTAAAAGCGGCGATCCATCACCATTTACAGCACTAGGTACCTATCTAGGCATTAAAGCCGCTGTTAAG
CATCAGCGTGGGCTCGATAGCCTCAAGGGACTAAAGATCTCAGTTCAAGGTGTTGGCCATGTAGGTTACTATCTATGTCG
CCATCTTCATGCCGAAGGTGCAGAGCTAATTGTGACAGACATTCATCAAGCATCACTAGACAGAGTCGCAGAAGAATTTG
GTGCAACAGTTGTCTCCCCTCAAGATATTTATCATCAGGATGTGGATGTCTACGCTCCTTGTGCGTTAGGCGCAACCATT
AATGACATAACCATCCCACTGCTAAAAGCGACAATTGTGGCAGGCTGTGCGAACAACCAACTTGGCGAAGTTCGTCACGG
TGAGATGCTAAAAGACTTGGGTATTTTGTACGCACCCGATTATGTTATTAACGCAGGCGGGATCATCAACGTATCTTTCG
AGAAAGATTACGATGCAACAGCTGCTACTGCAAAAGTTGAAGAGATCTACAATACCTTACTGACGATTTTCGCCCAGTCT
GATGAGCAAGACCGCACCACTGGTGATGTAGCGGATGAAATGGCACGAGCGATTATTCACGCTGCTAAATAA

Upstream 100 bases:

>100_bases
AGTATCCCTAAATGCTATCGTTCTGCAATAAATATAACAAGCAGACCGCTCTGTATTCGTACAGTTGCGGTAATTCGATG
CTCAAAGCGGAGATCAACAA

Downstream 100 bases:

>100_bases
ACAGACAATACATTAAAAATACCCAGCTTTTTTTAAGCTGGGTATTTTTTTGCTTGCAATGCCTAAGTTCAACAAATAGA
TAAGGGATTATCCCACCTCA

Product: Glu/Leu/Phe/Val dehydrogenase dimerisation region

Products: NA

Alternate protein names: LeuDH [H]

Number of amino acids: Translated: 343; Mature: 342

Protein sequence:

>343_residues
MAVFNHISFDDHEQVVFCHDKESGLKAIIAIHNTNLGPAVGGCRMWNYESDDEAITDVLRLSRGMTYKNALAGLAMGGGK
SVIIADPKVQDREALFRAFGRCIHTLGGKYYSAEDVGVSTSDIMIAHQETPYMAGLEGKSGDPSPFTALGTYLGIKAAVK
HQRGLDSLKGLKISVQGVGHVGYYLCRHLHAEGAELIVTDIHQASLDRVAEEFGATVVSPQDIYHQDVDVYAPCALGATI
NDITIPLLKATIVAGCANNQLGEVRHGEMLKDLGILYAPDYVINAGGIINVSFEKDYDATAATAKVEEIYNTLLTIFAQS
DEQDRTTGDVADEMARAIIHAAK

Sequences:

>Translated_343_residues
MAVFNHISFDDHEQVVFCHDKESGLKAIIAIHNTNLGPAVGGCRMWNYESDDEAITDVLRLSRGMTYKNALAGLAMGGGK
SVIIADPKVQDREALFRAFGRCIHTLGGKYYSAEDVGVSTSDIMIAHQETPYMAGLEGKSGDPSPFTALGTYLGIKAAVK
HQRGLDSLKGLKISVQGVGHVGYYLCRHLHAEGAELIVTDIHQASLDRVAEEFGATVVSPQDIYHQDVDVYAPCALGATI
NDITIPLLKATIVAGCANNQLGEVRHGEMLKDLGILYAPDYVINAGGIINVSFEKDYDATAATAKVEEIYNTLLTIFAQS
DEQDRTTGDVADEMARAIIHAAK
>Mature_342_residues
AVFNHISFDDHEQVVFCHDKESGLKAIIAIHNTNLGPAVGGCRMWNYESDDEAITDVLRLSRGMTYKNALAGLAMGGGKS
VIIADPKVQDREALFRAFGRCIHTLGGKYYSAEDVGVSTSDIMIAHQETPYMAGLEGKSGDPSPFTALGTYLGIKAAVKH
QRGLDSLKGLKISVQGVGHVGYYLCRHLHAEGAELIVTDIHQASLDRVAEEFGATVVSPQDIYHQDVDVYAPCALGATIN
DITIPLLKATIVAGCANNQLGEVRHGEMLKDLGILYAPDYVINAGGIINVSFEKDYDATAATAKVEEIYNTLLTIFAQSD
EQDRTTGDVADEMARAIIHAAK

Specific function: Functions catabolically in the bacterial metabolism of branched-chain L-amino acids, and plays an important role in spore germination in cooperation with alanine dehydrogenase [H]

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

None

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR016211
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.9 [H]

Molecular weight: Translated: 36940; Mature: 36809

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVFNHISFDDHEQVVFCHDKESGLKAIIAIHNTNLGPAVGGCRMWNYESDDEAITDVLR
CCEECCCCCCCCCCEEEEECCCCCCEEEEEEECCCCCCCCCCCEECCCCCCHHHHHHHHH
LSRGMTYKNALAGLAMGGGKSVIIADPKVQDREALFRAFGRCIHTLGGKYYSAEDVGVST
HHCCCCHHHHHHHHEECCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCEEECCCCCCCC
SDIMIAHQETPYMAGLEGKSGDPSPFTALGTYLGIKAAVKHQRGLDSLKGLKISVQGVGH
CCEEEEECCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHCCCEEEEEECCH
VGYYLCRHLHAEGAELIVTDIHQASLDRVAEEFGATVVSPQDIYHQDVDVYAPCALGATI
HHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHCCEECCHHHHHHCCCCEEECEECCCCH
NDITIPLLKATIVAGCANNQLGEVRHGEMLKDLGILYAPDYVINAGGIINVSFEKDYDAT
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCEECCCEEEECCCEEEEEECCCCCCH
AATAKVEEIYNTLLTIFAQSDEQDRTTGDVADEMARAIIHAAK
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AVFNHISFDDHEQVVFCHDKESGLKAIIAIHNTNLGPAVGGCRMWNYESDDEAITDVLR
CEECCCCCCCCCCEEEEECCCCCCEEEEEEECCCCCCCCCCCEECCCCCCHHHHHHHHH
LSRGMTYKNALAGLAMGGGKSVIIADPKVQDREALFRAFGRCIHTLGGKYYSAEDVGVST
HHCCCCHHHHHHHHEECCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCEEECCCCCCCC
SDIMIAHQETPYMAGLEGKSGDPSPFTALGTYLGIKAAVKHQRGLDSLKGLKISVQGVGH
CCEEEEECCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHCCCEEEEEECCH
VGYYLCRHLHAEGAELIVTDIHQASLDRVAEEFGATVVSPQDIYHQDVDVYAPCALGATI
HHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHCCEECCHHHHHHCCCCEEECEECCCCH
NDITIPLLKATIVAGCANNQLGEVRHGEMLKDLGILYAPDYVINAGGIINVSFEKDYDAT
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCEECCCEEEECCCEEEEEECCCCCCH
AATAKVEEIYNTLLTIFAQSDEQDRTTGDVADEMARAIIHAAK
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3069133; 1400267 [H]