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The map label for this gene is purB [H]

Identifier: 157962361

GI number: 157962361

Start: 3091320

End: 3092690

Strand: Direct

Name: purB [H]

Synonym: Spea_2540

Alternate gene names: 157962361

Gene position: 3091320-3092690 (Clockwise)

Preceding gene: 157962360

Following gene: 157962362

Centisome position: 59.74

GC content: 44.86

Gene sequence:

>1371_bases
ATGGAACTTTCCGCACTAACTGCTATCTCTCCGGTAGACGGTCGTTATGGTAGTAAAACCGCCTCATTAAGAGGGATTTT
CAGCGAGTACGGCCTGACCAAATACCGTGTTCAAGTCGAAATTAACTGGCTAAAGCTACTTTCTAGCTGCCCAGAAATTG
AAGAAGTTCCACCTTTTAGCGAAGCTGCATTGGCTTTGCTTGACCAAATTAAAGATAACTTTAGCGAAGCAGACGCACTA
CGCGTTAAAGATATTGAAGCCACCACCAACCATGATGTTAAAGCGGTTGAATACTTCATCAAAGAACGTATCGCTGATAA
TGCTGAGCTAGTGGCTATCGATGAGTTTGTACACTTTGCTTGTACCTCTGAAGACATTAATAACCTATCTCACGCATTAA
TGCTGACTGAAGCGCGCGACCAAGTACTTGTGCCGTATTGCCAGAAAGTTGTTGATGCAGTTAAAGCATTAGCACACGAG
CATAAGTCTGTACCGTTAATGTCTCGTACACACGGTCAACCCGCTTCACCGTCTACATTAGGTAAAGAGATGGCAAACGT
GGCTGTGCGTTTAGAGCGTCAGCTATCTCAAGTGTCTAAAGTTGAAATCATGGGCAAGATCAATGGTGCTGTTGGTAACT
ACAACGCTCACCTATCTGCTTATCCTGAAGTTGATTGGCACGCTCTGTCACAGCGTTTCGTGACAAGCCTTGGCATTAAC
TGGAACCCATACACGACTCAAATTGAGCCACACGATTACATCGCTGAATTGTTTGATGCGCTAGCGCGCTTCAACACAAT
CCTTATCGATTTCGACCGTGATATTTGGGGTTACATCGCGCTTGGTCACTTTAAGCAAAAGACCATTGCTGGTGAAATTG
GCTCTTCAACCATGCCACATAAAGTCAACCCAATCGACTTCGAAAACTCTGAAGGTAACTTAGGTATTGCTAACGCGTTA
ATGCAGCACCTTGCAGCCAAGCTTCCTGTTTCACGCTGGCAGCGTGACCTTACAGACTCTACAGTGCTACGTAACTTAGG
TGTGGGTATGGCTCACTCTCTAATCGCTTACCAATCAACATTGAAAGGGATCAGTAAGCTAGAAGTTAACGAAGAGCAGC
TTCGTAAAGAGCTTGATGGTAACTGGGAAGTACTAGCTGAGCCAGTACAAACCGTAATGCGTCGTTATGGCATCGAGAAA
CCATATGAAAAGCTTAAAGAGTTGACTCGCGGTAAACGTATCGATGGTGCTCAACTTGCAACATTCATCGACGGTCTTGA
GCTACCAGAAGACGTTAAAATCGAACTTAAGAAGATGACGCCTGCCAACTATATTGGCCGTGCAGAAGCGTTTGTTGACG
AGCTAAACTAA

Upstream 100 bases:

>100_bases
AAGCGTAGGCACCTTGTCTTCTCAAGAAAAACAATAGTAGATACAGCTATGAAAAGCCTCACCCTATAATATTTAAGTCA
AGTTCATCAAGGAGCTTCTA

Downstream 100 bases:

>100_bases
TAGCAGACTCAACACTTTAGATAGCTCTTCGGCTATTAAAGGTTCGAATAAGGCGTTAAGCTTAGGCTTAACGCCTTTAT
TTTATCCATTTTCCGTCGAG

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MELSALTAISPVDGRYGSKTASLRGIFSEYGLTKYRVQVEINWLKLLSSCPEIEEVPPFSEAALALLDQIKDNFSEADAL
RVKDIEATTNHDVKAVEYFIKERIADNAELVAIDEFVHFACTSEDINNLSHALMLTEARDQVLVPYCQKVVDAVKALAHE
HKSVPLMSRTHGQPASPSTLGKEMANVAVRLERQLSQVSKVEIMGKINGAVGNYNAHLSAYPEVDWHALSQRFVTSLGIN
WNPYTTQIEPHDYIAELFDALARFNTILIDFDRDIWGYIALGHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGIANAL
MQHLAAKLPVSRWQRDLTDSTVLRNLGVGMAHSLIAYQSTLKGISKLEVNEEQLRKELDGNWEVLAEPVQTVMRRYGIEK
PYEKLKELTRGKRIDGAQLATFIDGLELPEDVKIELKKMTPANYIGRAEAFVDELN

Sequences:

>Translated_456_residues
MELSALTAISPVDGRYGSKTASLRGIFSEYGLTKYRVQVEINWLKLLSSCPEIEEVPPFSEAALALLDQIKDNFSEADAL
RVKDIEATTNHDVKAVEYFIKERIADNAELVAIDEFVHFACTSEDINNLSHALMLTEARDQVLVPYCQKVVDAVKALAHE
HKSVPLMSRTHGQPASPSTLGKEMANVAVRLERQLSQVSKVEIMGKINGAVGNYNAHLSAYPEVDWHALSQRFVTSLGIN
WNPYTTQIEPHDYIAELFDALARFNTILIDFDRDIWGYIALGHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGIANAL
MQHLAAKLPVSRWQRDLTDSTVLRNLGVGMAHSLIAYQSTLKGISKLEVNEEQLRKELDGNWEVLAEPVQTVMRRYGIEK
PYEKLKELTRGKRIDGAQLATFIDGLELPEDVKIELKKMTPANYIGRAEAFVDELN
>Mature_456_residues
MELSALTAISPVDGRYGSKTASLRGIFSEYGLTKYRVQVEINWLKLLSSCPEIEEVPPFSEAALALLDQIKDNFSEADAL
RVKDIEATTNHDVKAVEYFIKERIADNAELVAIDEFVHFACTSEDINNLSHALMLTEARDQVLVPYCQKVVDAVKALAHE
HKSVPLMSRTHGQPASPSTLGKEMANVAVRLERQLSQVSKVEIMGKINGAVGNYNAHLSAYPEVDWHALSQRFVTSLGIN
WNPYTTQIEPHDYIAELFDALARFNTILIDFDRDIWGYIALGHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGIANAL
MQHLAAKLPVSRWQRDLTDSTVLRNLGVGMAHSLIAYQSTLKGISKLEVNEEQLRKELDGNWEVLAEPVQTVMRRYGIEK
PYEKLKELTRGKRIDGAQLATFIDGLELPEDVKIELKKMTPANYIGRAEAFVDELN

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787376, Length=455, Percent_Identity=74.0659340659341, Blast_Score=728, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013539
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF08328 ASL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 50991; Mature: 50991

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELSALTAISPVDGRYGSKTASLRGIFSEYGLTKYRVQVEINWLKLLSSCPEIEEVPPFS
CCCCCCCEECCCCCCCCCCHHHHHHHHHHCCCEEEEEEEEEHHHHHHHCCCCCCCCCCHH
EAALALLDQIKDNFSEADALRVKDIEATTNHDVKAVEYFIKERIADNAELVAIDEFVHFA
HHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCEEEHHHHHHHH
CTSEDINNLSHALMLTEARDQVLVPYCQKVVDAVKALAHEHKSVPLMSRTHGQPASPSTL
CCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCHHHH
GKEMANVAVRLERQLSQVSKVEIMGKINGAVGNYNAHLSAYPEVDWHALSQRFVTSLGIN
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCHHHHHHHHHHHHHCCC
WNPYTTQIEPHDYIAELFDALARFNTILIDFDRDIWGYIALGHFKQKTIAGEIGSSTMPH
CCCCCCCCCCHHHHHHHHHHHHHHCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCC
KVNPIDFENSEGNLGIANALMQHLAAKLPVSRWQRDLTDSTVLRNLGVGMAHSLIAYQST
CCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
LKGISKLEVNEEQLRKELDGNWEVLAEPVQTVMRRYGIEKPYEKLKELTRGKRIDGAQLA
HHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHH
TFIDGLELPEDVKIELKKMTPANYIGRAEAFVDELN
HHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MELSALTAISPVDGRYGSKTASLRGIFSEYGLTKYRVQVEINWLKLLSSCPEIEEVPPFS
CCCCCCCEECCCCCCCCCCHHHHHHHHHHCCCEEEEEEEEEHHHHHHHCCCCCCCCCCHH
EAALALLDQIKDNFSEADALRVKDIEATTNHDVKAVEYFIKERIADNAELVAIDEFVHFA
HHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCEEEHHHHHHHH
CTSEDINNLSHALMLTEARDQVLVPYCQKVVDAVKALAHEHKSVPLMSRTHGQPASPSTL
CCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCHHHH
GKEMANVAVRLERQLSQVSKVEIMGKINGAVGNYNAHLSAYPEVDWHALSQRFVTSLGIN
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCHHHHHHHHHHHHHCCC
WNPYTTQIEPHDYIAELFDALARFNTILIDFDRDIWGYIALGHFKQKTIAGEIGSSTMPH
CCCCCCCCCCHHHHHHHHHHHHHHCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCC
KVNPIDFENSEGNLGIANALMQHLAAKLPVSRWQRDLTDSTVLRNLGVGMAHSLIAYQST
CCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
LKGISKLEVNEEQLRKELDGNWEVLAEPVQTVMRRYGIEKPYEKLKELTRGKRIDGAQLA
HHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHH
TFIDGLELPEDVKIELKKMTPANYIGRAEAFVDELN
HHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]