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The map label for this gene is minD [H]

Identifier: 157962266

GI number: 157962266

Start: 2988442

End: 2989251

Strand: Direct

Name: minD [H]

Synonym: Spea_2445

Alternate gene names: 157962266

Gene position: 2988442-2989251 (Clockwise)

Preceding gene: 157962265

Following gene: 157962267

Centisome position: 57.75

GC content: 43.46

Gene sequence:

>810_bases
ATGGCACAGATTATTGTTGTCACATCGGGTAAAGGTGGCGTGGGTAAAACCACCTCAAGTGCTGCAATTGCCACAGGTCT
TGCAATGAAAGGCCATAAAACGGTTGTTATCGATTTTGATATTGGCCTCCGTAACCTAGATTTAATCATGGGCTGTGAAC
GCCGCGTAGTCTATGACTTTGTTAACGTCATCAATGGTGAAGCTAATCTTAATCAGGCCTTGATCAAAGATAAGCGCTGC
GCCAATCTTTTCGTATTACCCGCTTCCCAAACTCGTGATAAAGATGCCTTGACTAAAGAAGGCGTGGGTAAGGTACTTGA
AGATTTAGCCAAAGATTTTGAGTACATTATTTGTGACTCACCAGCGGGTATCGAAACCGGCGCTATGATGGCGCTTTATT
TTGCTGATACTGCGATTGTAACAACCAACCCAGAAGTCAGCTCTGTTCGCGACTCAGACCGAATTCTTGGTATGTTACAG
AGTAAATCTAAGCGTGCAGAAGAAGGGTTAGAGCCCGTCAAAGAGTATTTACTATTGACTCGTTACTCTCCCGCTCGTGT
GACTACTGGCGAAATGCTAAGTGTTCAAGATGTTGAAGAGATCTTAGCGATTCCACTATTGGGCGTTATCCCGGAATCAC
AAGCCGTATTAAAAGCCTCTAACTCTGGTATACCTGTCATTATCGATCAAGAAAGTGATGCAGGTATGGCCTACAGCGAT
GCTGTAGAAAGGTTATTAGGTGAAGAACTGCCTTTCCGATTTCTTACGGAAGAGAAGAAAGGTTTCTTAAAACGAATTTT
TGGTAGCTAA

Upstream 100 bases:

>100_bases
GGAGCCGCACCAAGCGGTTGTATTCGTCTAGAAGGCGAGTCGCTTACGGTTGAATCATTGCCTCTTTAAATAGAGTGTAA
ATTAGAAAGGATTATTATAA

Downstream 100 bases:

>100_bases
CTTATGTCTCTTCTTGATTATTTTAAAACGAAAAAGGAACCAAACACTGCGGTCACAGCGAAAGAACGACTGCAAATTAT
CGTGGCTCACCAGAGAGGGG

Product: septum site-determining protein MinD

Products: NA

Alternate protein names: Cell division inhibitor minD [H]

Number of amino acids: Translated: 269; Mature: 268

Protein sequence:

>269_residues
MAQIIVVTSGKGGVGKTTSSAAIATGLAMKGHKTVVIDFDIGLRNLDLIMGCERRVVYDFVNVINGEANLNQALIKDKRC
ANLFVLPASQTRDKDALTKEGVGKVLEDLAKDFEYIICDSPAGIETGAMMALYFADTAIVTTNPEVSSVRDSDRILGMLQ
SKSKRAEEGLEPVKEYLLLTRYSPARVTTGEMLSVQDVEEILAIPLLGVIPESQAVLKASNSGIPVIIDQESDAGMAYSD
AVERLLGEELPFRFLTEEKKGFLKRIFGS

Sequences:

>Translated_269_residues
MAQIIVVTSGKGGVGKTTSSAAIATGLAMKGHKTVVIDFDIGLRNLDLIMGCERRVVYDFVNVINGEANLNQALIKDKRC
ANLFVLPASQTRDKDALTKEGVGKVLEDLAKDFEYIICDSPAGIETGAMMALYFADTAIVTTNPEVSSVRDSDRILGMLQ
SKSKRAEEGLEPVKEYLLLTRYSPARVTTGEMLSVQDVEEILAIPLLGVIPESQAVLKASNSGIPVIIDQESDAGMAYSD
AVERLLGEELPFRFLTEEKKGFLKRIFGS
>Mature_268_residues
AQIIVVTSGKGGVGKTTSSAAIATGLAMKGHKTVVIDFDIGLRNLDLIMGCERRVVYDFVNVINGEANLNQALIKDKRCA
NLFVLPASQTRDKDALTKEGVGKVLEDLAKDFEYIICDSPAGIETGAMMALYFADTAIVTTNPEVSSVRDSDRILGMLQS
KSKRAEEGLEPVKEYLLLTRYSPARVTTGEMLSVQDVEEILAIPLLGVIPESQAVLKASNSGIPVIIDQESDAGMAYSDA
VERLLGEELPFRFLTEEKKGFLKRIFGS

Specific function: ATPase required for the correct placement of the division site. Cell division inhibitors minC and minD act in concert to form an inhibitor capable of blocking formation of the polar Z ring septums. Rapidly oscillates between the poles of the cell to desta

COG id: COG2894

COG function: function code D; Septum formation inhibitor-activating ATPase

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the parA family. MinD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787423, Length=269, Percent_Identity=79.9256505576208, Blast_Score=438, Evalue=1e-124,

Paralogues:

None

Copy number: 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002586
- InterPro:   IPR010223 [H]

Pfam domain/function: PF01656 CbiA [H]

EC number: NA

Molecular weight: Translated: 29136; Mature: 29005

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQIIVVTSGKGGVGKTTSSAAIATGLAMKGHKTVVIDFDIGLRNLDLIMGCERRVVYDF
CEEEEEEECCCCCCCCCCCCHHHHHHEEECCCEEEEEEECCCCCCCHHHHCCCHHHHHHH
VNVINGEANLNQALIKDKRCANLFVLPASQTRDKDALTKEGVGKVLEDLAKDFEYIICDS
HHHHCCCCCCHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEECC
PAGIETGAMMALYFADTAIVTTNPEVSSVRDSDRILGMLQSKSKRAEEGLEPVKEYLLLT
CCCCCCCCEEEEEEECEEEEECCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
RYSPARVTTGEMLSVQDVEEILAIPLLGVIPESQAVLKASNSGIPVIIDQESDAGMAYSD
CCCCCEECCCCEECHHHHHHHHHHHHHHCCCCCCHHEEECCCCCCEEEECCCCCCCHHHH
AVERLLGEELPFRFLTEEKKGFLKRIFGS
HHHHHHCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AQIIVVTSGKGGVGKTTSSAAIATGLAMKGHKTVVIDFDIGLRNLDLIMGCERRVVYDF
EEEEEEECCCCCCCCCCCCHHHHHHEEECCCEEEEEEECCCCCCCHHHHCCCHHHHHHH
VNVINGEANLNQALIKDKRCANLFVLPASQTRDKDALTKEGVGKVLEDLAKDFEYIICDS
HHHHCCCCCCHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEECC
PAGIETGAMMALYFADTAIVTTNPEVSSVRDSDRILGMLQSKSKRAEEGLEPVKEYLLLT
CCCCCCCCEEEEEEECEEEEECCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
RYSPARVTTGEMLSVQDVEEILAIPLLGVIPESQAVLKASNSGIPVIIDQESDAGMAYSD
CCCCCEECCCCEECHHHHHHHHHHHHHHCCCCCCHHEEECCCCCCEEEECCCCCCCHHHH
AVERLLGEELPFRFLTEEKKGFLKRIFGS
HHHHHHCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]