Click here to switch to the map view.

The map label for this gene is eco [H]

Identifier: 157962150

GI number: 157962150

Start: 2838728

End: 2839309

Strand: Direct

Name: eco [H]

Synonym: Spea_2329

Alternate gene names: 157962150

Gene position: 2838728-2839309 (Clockwise)

Preceding gene: 157962145

Following gene: 157962157

Centisome position: 54.86

GC content: 40.89

Gene sequence:

>582_bases
ATGACTTTATTCACTGCTGCAAACAAACATTTTGCTCGTACTCTTTTAGCTTCTTCTTTAATTCTTTCGGCTTTTTCATT
CAATGCGAGTGCGACTAGCCCAGCCCATCCATCTGGATTAAATCAAAATATGATCAGTGCACAAATGTATAGCGCGAGTG
ATTACACTGCGACCAAAGAAACAAAAATGTATCCAAAACCAGAAGCGGGTCAAGTCCAGCATATCTTGACTCTACCTAAG
CTAGATAATGAAGACGATTACAGAGTGGAGATCCAGATCGGTCAAACAAAGATGGTCGACTGTAACAAGCATGGTTTATC
AGGTGAACTCAAACAACATTCGGTCAAAGGATGGGGCTATAACTATTATCAAGTAGACACCATTAGCGATGGTCCAAGCA
CTATGATGGCCTGCTTCGAACAAACTAAAACGGAAAAATTCTTACCTATTGGTGATGATCTAATGCTTAAGTACGATAGT
CGCTTACCGAAAGTCTTTTATCTACCAGAAAACACTCAGGTGCGTTATCGCGTCTGGAAAGCCGAATCAGCATTTAACTA
TTCGGGTGAAGCGGCACAATAA

Upstream 100 bases:

>100_bases
CAGGCTGAGTATTTTTTTTGTTTTTTTTAACCCTTTTTGAAAACGACCCCGTTTATAGAGGTATTGAACACAGACAATAC
CTTTTTCTTTTGGAGATTTT

Downstream 100 bases:

>100_bases
TCAGCTTCGATTTCTGAAGTGTGTCTAGTCCTAAAATAGGTTGACGGTTTTTATTAAGCCAGTTGGAACTCCCGACTGGC
TTTTTTATGTACTTCATTAG

Product: ecotin

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 193; Mature: 192

Protein sequence:

>193_residues
MTLFTAANKHFARTLLASSLILSAFSFNASATSPAHPSGLNQNMISAQMYSASDYTATKETKMYPKPEAGQVQHILTLPK
LDNEDDYRVEIQIGQTKMVDCNKHGLSGELKQHSVKGWGYNYYQVDTISDGPSTMMACFEQTKTEKFLPIGDDLMLKYDS
RLPKVFYLPENTQVRYRVWKAESAFNYSGEAAQ

Sequences:

>Translated_193_residues
MTLFTAANKHFARTLLASSLILSAFSFNASATSPAHPSGLNQNMISAQMYSASDYTATKETKMYPKPEAGQVQHILTLPK
LDNEDDYRVEIQIGQTKMVDCNKHGLSGELKQHSVKGWGYNYYQVDTISDGPSTMMACFEQTKTEKFLPIGDDLMLKYDS
RLPKVFYLPENTQVRYRVWKAESAFNYSGEAAQ
>Mature_192_residues
TLFTAANKHFARTLLASSLILSAFSFNASATSPAHPSGLNQNMISAQMYSASDYTATKETKMYPKPEAGQVQHILTLPKL
DNEDDYRVEIQIGQTKMVDCNKHGLSGELKQHSVKGWGYNYYQVDTISDGPSTMMACFEQTKTEKFLPIGDDLMLKYDSR
LPKVFYLPENTQVRYRVWKAESAFNYSGEAAQ

Specific function: General inhibitor of family S1 serine proteases [H]

COG id: COG4574

COG function: function code R; Serine protease inhibitor ecotin

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the protease inhibitor I11 (ecotin) family [H]

Homologues:

Organism=Escherichia coli, GI1788538, Length=168, Percent_Identity=39.2857142857143, Blast_Score=124, Evalue=5e-30,

Paralogues:

None

Copy number: 260 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005658
- InterPro:   IPR023189
- InterPro:   IPR023084 [H]

Pfam domain/function: PF03974 Ecotin [H]

EC number: NA

Molecular weight: Translated: 21703; Mature: 21572

Theoretical pI: Translated: 7.05; Mature: 7.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLFTAANKHFARTLLASSLILSAFSFNASATSPAHPSGLNQNMISAQMYSASDYTATKE
CEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCC
TKMYPKPEAGQVQHILTLPKLDNEDDYRVEIQIGQTKMVDCNKHGLSGELKQHSVKGWGY
CCCCCCCCCCCEEEEEECCCCCCCCCEEEEEEECCEEEEECCCCCCCCCHHHCCCCCCCC
NYYQVDTISDGPSTMMACFEQTKTEKFLPIGDDLMLKYDSRLPKVFYLPENTQVRYRVWK
CEEEEEEECCCHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCEEEECCCCCEEEEEEEE
AESAFNYSGEAAQ
CCCCCCCCCCCCC
>Mature Secondary Structure 
TLFTAANKHFARTLLASSLILSAFSFNASATSPAHPSGLNQNMISAQMYSASDYTATKE
EEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCC
TKMYPKPEAGQVQHILTLPKLDNEDDYRVEIQIGQTKMVDCNKHGLSGELKQHSVKGWGY
CCCCCCCCCCCEEEEEECCCCCCCCCEEEEEEECCEEEEECCCCCCCCCHHHCCCCCCCC
NYYQVDTISDGPSTMMACFEQTKTEKFLPIGDDLMLKYDSRLPKVFYLPENTQVRYRVWK
CEEEEEEECCCHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCEEEECCCCCEEEEEEEE
AESAFNYSGEAAQ
CCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]