Click here to switch to the map view.

The map label for this gene is degA [H]

Identifier: 157962108

GI number: 157962108

Start: 2770396

End: 2771385

Strand: Direct

Name: degA [H]

Synonym: Spea_2287

Alternate gene names: 157962108

Gene position: 2770396-2771385 (Clockwise)

Preceding gene: 157962102

Following gene: 157962109

Centisome position: 53.54

GC content: 42.63

Gene sequence:

>990_bases
ATGAAAAAAGTTAAGCTATCAGATATTGCCAAGATGGCTGGTGTATCCTCGATTACCGTCAGTAGAGCGTTATCAACGCC
TGAAAAGGTCAAAGCTGATACCTGTGAAAAGATCCAGAAAATAGCGCAAGAGCTTGGCTATATACCCAACCTAATGGCAA
AAGGGCTTAAGTCTCGATCAAACACCATAGGGGTTATTGTTCCGACAATTATCAACCCGTTTTTTGCCAGTGCGGTAAAG
TCCATCATTCGTAGTGCCAATAAAACAGGTTATAACTGCCTATTCTTCACCTCAGATGAATCGCAAAAAATAGAGCAAAA
CGCAGTCAATACGCTAATTAGTTACAATATCGACGGGATCATCATTGCCGTTATTTCAGAAGATGAAAACTATACTCCCG
CTTACTTTTCAACCTTAGAGAAGTTACACGTCCCAGTGGTATTGCTCGACAGACATATCGAGGCGCCACATATTTGCGGA
GTGTATTTAGATAATGTCGATTCAGGCTACAAGCTTGGCAAAGAAGTGTTATTAAACAATTACCAACGGGTGTTAATCGT
ATCCGGCTCAGCCAACTCTAAAGTGGCCAATTCACGATTAGCAGGCCTTAAGATGGCAGCGGAAGAGACACATTCAAATA
CGATTATCGATGTGATCAAGGCGGACTTTAATACCCAATTGGCAAAGGAATTAGTGCAAGATTACCTATCACAGCATAAA
CCCGATGCCATTATTGGTTTAAACAACCAGATCACCTTAGGTGCGCTTGAGGCCAGTGTTTCACATCACTATCAACTTGG
AGCGGATATTCATTTCTATAGTATCGATGAAATCACCACCGCAAAACACTTTGGCATCTCCATCCCCTGTATCAGGCATG
ACGTTGAAGAGTTTGGCTACCATGCCGTGACGCAGTTAATCCGGGCCATTGAGTCTGAGCAGTGCGACAAACTGTCTGAT
ATCATCATTAGAGGCACGGTAGTTAAGTAA

Upstream 100 bases:

>100_bases
CATAACATTGGCTTGAGTAAATCTAGGACTAGGAAGTAATACGCGGATATTGCCTCTACCTAAATGAAGAGAACGGTAAA
TGACGCGGGAACTAAAGAGC

Downstream 100 bases:

>100_bases
GTCCCTTATCCGTACTGTATTTGAATCTCAATCAACATCGATATAAACCTGCTGTTTGAACCTCCAGATTAAATCTGCTG
ATTAAACCTATGGCCCTGAA

Product: LacI family transcription regulator

Products: NA

Alternate protein names: Degradation activator [H]

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MKKVKLSDIAKMAGVSSITVSRALSTPEKVKADTCEKIQKIAQELGYIPNLMAKGLKSRSNTIGVIVPTIINPFFASAVK
SIIRSANKTGYNCLFFTSDESQKIEQNAVNTLISYNIDGIIIAVISEDENYTPAYFSTLEKLHVPVVLLDRHIEAPHICG
VYLDNVDSGYKLGKEVLLNNYQRVLIVSGSANSKVANSRLAGLKMAAEETHSNTIIDVIKADFNTQLAKELVQDYLSQHK
PDAIIGLNNQITLGALEASVSHHYQLGADIHFYSIDEITTAKHFGISIPCIRHDVEEFGYHAVTQLIRAIESEQCDKLSD
IIIRGTVVK

Sequences:

>Translated_329_residues
MKKVKLSDIAKMAGVSSITVSRALSTPEKVKADTCEKIQKIAQELGYIPNLMAKGLKSRSNTIGVIVPTIINPFFASAVK
SIIRSANKTGYNCLFFTSDESQKIEQNAVNTLISYNIDGIIIAVISEDENYTPAYFSTLEKLHVPVVLLDRHIEAPHICG
VYLDNVDSGYKLGKEVLLNNYQRVLIVSGSANSKVANSRLAGLKMAAEETHSNTIIDVIKADFNTQLAKELVQDYLSQHK
PDAIIGLNNQITLGALEASVSHHYQLGADIHFYSIDEITTAKHFGISIPCIRHDVEEFGYHAVTQLIRAIESEQCDKLSD
IIIRGTVVK
>Mature_329_residues
MKKVKLSDIAKMAGVSSITVSRALSTPEKVKADTCEKIQKIAQELGYIPNLMAKGLKSRSNTIGVIVPTIINPFFASAVK
SIIRSANKTGYNCLFFTSDESQKIEQNAVNTLISYNIDGIIIAVISEDENYTPAYFSTLEKLHVPVVLLDRHIEAPHICG
VYLDNVDSGYKLGKEVLLNNYQRVLIVSGSANSKVANSRLAGLKMAAEETHSNTIIDVIKADFNTQLAKELVQDYLSQHK
PDAIIGLNNQITLGALEASVSHHYQLGADIHFYSIDEITTAKHFGISIPCIRHDVEEFGYHAVTQLIRAIESEQCDKLSD
IIIRGTVVK

Specific function: Involved in the control of degradation of B.subtilis amidophosphoribosyltransferase (purF). Probably activates the gene for a degradative protease [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790369, Length=341, Percent_Identity=25.8064516129032, Blast_Score=104, Evalue=7e-24,
Organism=Escherichia coli, GI1790715, Length=258, Percent_Identity=29.8449612403101, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1790194, Length=313, Percent_Identity=26.1980830670927, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1787948, Length=310, Percent_Identity=22.5806451612903, Blast_Score=94, Evalue=9e-21,
Organism=Escherichia coli, GI1786268, Length=272, Percent_Identity=23.5294117647059, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI48994940, Length=185, Percent_Identity=29.7297297297297, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1786540, Length=313, Percent_Identity=27.4760383386581, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1788474, Length=212, Percent_Identity=33.9622641509434, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1789068, Length=312, Percent_Identity=24.0384615384615, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1787580, Length=320, Percent_Identity=25.9375, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1787906, Length=266, Percent_Identity=25.9398496240602, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1789202, Length=207, Percent_Identity=24.1545893719807, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36188; Mature: 36188

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVKLSDIAKMAGVSSITVSRALSTPEKVKADTCEKIQKIAQELGYIPNLMAKGLKSRS
CCCCCHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCC
NTIGVIVPTIINPFFASAVKSIIRSANKTGYNCLFFTSDESQKIEQNAVNTLISYNIDGI
CEEEEEEHHHHCHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCEE
IIAVISEDENYTPAYFSTLEKLHVPVVLLDRHIEAPHICGVYLDNVDSGYKLGKEVLLNN
EEEEEECCCCCCCHHHHHHHHHCCCEEEECCCCCCCCEEEEEECCCCCHHHHHHHHHHCC
YQRVLIVSGSANSKVANSRLAGLKMAAEETHSNTIIDVIKADFNTQLAKELVQDYLSQHK
CEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEEHHHCCCCHHHHHHHHHHHHHHCC
PDAIIGLNNQITLGALEASVSHHYQLGADIHFYSIDEITTAKHFGISIPCIRHDVEEFGY
CCEEEECCCCEEEEHHHHHHHHCEEECCCEEEEECCCHHHHHHCCCCCHHHHHHHHHHCH
HAVTQLIRAIESEQCDKLSDIIIRGTVVK
HHHHHHHHHHHHHHHHHHHHHHHEEEECC
>Mature Secondary Structure
MKKVKLSDIAKMAGVSSITVSRALSTPEKVKADTCEKIQKIAQELGYIPNLMAKGLKSRS
CCCCCHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCC
NTIGVIVPTIINPFFASAVKSIIRSANKTGYNCLFFTSDESQKIEQNAVNTLISYNIDGI
CEEEEEEHHHHCHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCEE
IIAVISEDENYTPAYFSTLEKLHVPVVLLDRHIEAPHICGVYLDNVDSGYKLGKEVLLNN
EEEEEECCCCCCCHHHHHHHHHCCCEEEECCCCCCCCEEEEEECCCCCHHHHHHHHHHCC
YQRVLIVSGSANSKVANSRLAGLKMAAEETHSNTIIDVIKADFNTQLAKELVQDYLSQHK
CEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEEHHHCCCCHHHHHHHHHHHHHHCC
PDAIIGLNNQITLGALEASVSHHYQLGADIHFYSIDEITTAKHFGISIPCIRHDVEEFGY
CCEEEECCCCEEEEHHHHHHHHCEEECCCEEEEECCCHHHHHHCCCCCHHHHHHHHHHCH
HAVTQLIRAIESEQCDKLSDIIIRGTVVK
HHHHHHHHHHHHHHHHHHHHHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8407808; 9353932; 9384377 [H]