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The map label for this gene is etrA [H]

Identifier: 157962043

GI number: 157962043

Start: 2694204

End: 2694956

Strand: Direct

Name: etrA [H]

Synonym: Spea_2222

Alternate gene names: 157962043

Gene position: 2694204-2694956 (Clockwise)

Preceding gene: 157962042

Following gene: 157962044

Centisome position: 52.07

GC content: 42.1

Gene sequence:

>753_bases
ATGTCAGCAGATAACAACAAGAATCGCCGTCAGATGGCGTCAGGATGTGCGATTCATTGTCATGATTGCAGCATGGGTAC
ACTTTGTATTCCTTTCACACTTAACTCCAATGAGTTAGATCAGCTTGATGAGATCATCGAACGTAAGAAGCCGATACAAA
AAGGCGAACCGATTTTTAAATCTGGCGATCCGCTAAAGTCTTTGTTTGCGATCCGCTCTGGCACAATCAAAAGTTATACC
ATTACAGAACAAGGCGATGAACAGATCACAGGCTTCCACCTCGCTGGCGATGTCATCGGTTTCGATGGGATTCATTCCCA
ATCTCATCAGAGTTTCGCGCAGGCCCTTGAAACCTCAATGGTTTGTGAAATCCCTTATACCACTCTCGACGAGCTAACAG
GTAAGATGCCTAAGCTTAGACAACAGATCATGCGCTTAATGAGTAATGAAATTCAAAGCGATCAAGAGATGATCCTGTTA
TTAAGCAAGAAGAATGCAGAAGAGAGACTCGCAGCCTTTATCAGTAATTTAGCTAAGCGCTTCGGCAGCAGAGGTTTTTC
ACCAAGAGAGTTTAGGTTAACCATGACTCGTGGTGATATTGGTAATTACCTTGGTCTGACCGTTGAAACAATTAGCCGCT
TACTTGGTCGCTTTCAAAAAGCAGAACTTATTGAAGTTAAAGGTAAATACATTACAATATTAGATATTGACGCATTAAGC
GAACTATCTGGTAACAGTAATATTGCGCGATAG

Upstream 100 bases:

>100_bases
CGGAGTACAAACCTTGTACGTGGCGATTAACCAGCTTAACTAGCTCCGATTATCAAATTAGTTTAACATAGTAGATGAAC
AGTCAAAATTGAGAGAGACA

Downstream 100 bases:

>100_bases
GATTTATTCGGCAATCAGCTTGATCTAAAACAATATAAATTCATCGCAATCATGCAAGATGAAACTAGACGATATTTAGA
TAAGGAAACGGCTATGATGG

Product: fumarate/nitrate reduction transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 250; Mature: 249

Protein sequence:

>250_residues
MSADNNKNRRQMASGCAIHCHDCSMGTLCIPFTLNSNELDQLDEIIERKKPIQKGEPIFKSGDPLKSLFAIRSGTIKSYT
ITEQGDEQITGFHLAGDVIGFDGIHSQSHQSFAQALETSMVCEIPYTTLDELTGKMPKLRQQIMRLMSNEIQSDQEMILL
LSKKNAEERLAAFISNLAKRFGSRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRFQKAELIEVKGKYITILDIDALS
ELSGNSNIAR

Sequences:

>Translated_250_residues
MSADNNKNRRQMASGCAIHCHDCSMGTLCIPFTLNSNELDQLDEIIERKKPIQKGEPIFKSGDPLKSLFAIRSGTIKSYT
ITEQGDEQITGFHLAGDVIGFDGIHSQSHQSFAQALETSMVCEIPYTTLDELTGKMPKLRQQIMRLMSNEIQSDQEMILL
LSKKNAEERLAAFISNLAKRFGSRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRFQKAELIEVKGKYITILDIDALS
ELSGNSNIAR
>Mature_249_residues
SADNNKNRRQMASGCAIHCHDCSMGTLCIPFTLNSNELDQLDEIIERKKPIQKGEPIFKSGDPLKSLFAIRSGTIKSYTI
TEQGDEQITGFHLAGDVIGFDGIHSQSHQSFAQALETSMVCEIPYTTLDELTGKMPKLRQQIMRLMSNEIQSDQEMILLL
SKKNAEERLAAFISNLAKRFGSRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRFQKAELIEVKGKYITILDIDALSE
LSGNSNIAR

Specific function: Regulates anaerobic growth on fumarate, nitrite, Fe(3+), TMAO, DMSO, thiosulfate and sulfite, but not on nitrate nor Mn(4+) [H]

COG id: COG0664

COG function: function code T; cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787595, Length=242, Percent_Identity=77.6859504132231, Blast_Score=392, Evalue=1e-110,
Organism=Escherichia coli, GI1789756, Length=178, Percent_Identity=25.8426966292135, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR000595
- InterPro:   IPR001808
- InterPro:   IPR012318
- InterPro:   IPR014710
- InterPro:   IPR018335
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00027 cNMP_binding; PF00325 Crp [H]

EC number: NA

Molecular weight: Translated: 27924; Mature: 27793

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: PS50042 CNMP_BINDING_3 ; PS00042 HTH_CRP_1 ; PS51063 HTH_CRP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSADNNKNRRQMASGCAIHCHDCSMGTLCIPFTLNSNELDQLDEIIERKKPIQKGEPIFK
CCCCCCCHHHHHHCCCEEEEECCCCCCEEEEEEECCCHHHHHHHHHHHCCCCCCCCCCCC
SGDPLKSLFAIRSGTIKSYTITEQGDEQITGFHLAGDVIGFDGIHSQSHQSFAQALETSM
CCCHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEECCEECCCCCCCHHHHHHHHHHHHHH
VCEIPYTTLDELTGKMPKLRQQIMRLMSNEIQSDQEMILLLSKKNAEERLAAFISNLAKR
EEECCCHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHH
FGSRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRFQKAELIEVKGKYITILDIDALS
HCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHEEEECCCEEEEEEHHHHH
ELSGNSNIAR
HCCCCCCCCC
>Mature Secondary Structure 
SADNNKNRRQMASGCAIHCHDCSMGTLCIPFTLNSNELDQLDEIIERKKPIQKGEPIFK
CCCCCCHHHHHHCCCEEEEECCCCCCEEEEEEECCCHHHHHHHHHHHCCCCCCCCCCCC
SGDPLKSLFAIRSGTIKSYTITEQGDEQITGFHLAGDVIGFDGIHSQSHQSFAQALETSM
CCCHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEECCEECCCCCCCHHHHHHHHHHHHHH
VCEIPYTTLDELTGKMPKLRQQIMRLMSNEIQSDQEMILLLSKKNAEERLAAFISNLAKR
EEECCCHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHH
FGSRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRFQKAELIEVKGKYITILDIDALS
HCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHEEEECCCEEEEEEHHHHH
ELSGNSNIAR
HCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8253682; 12368813 [H]