Click here to switch to the map view.

The map label for this gene is bioD [H]

Identifier: 157961628

GI number: 157961628

Start: 2203089

End: 2203754

Strand: Direct

Name: bioD [H]

Synonym: Spea_1804

Alternate gene names: 157961628

Gene position: 2203089-2203754 (Clockwise)

Preceding gene: 157961627

Following gene: 157961629

Centisome position: 42.58

GC content: 45.2

Gene sequence:

>666_bases
ATGACATTTTTTGTAACGGGTACAGATACCGACTGTGGTAAGACGTTGATCTCTTCTGCATTGTTAGCAGCAGCAAAAGG
CGAGACAGTAGGCTTTAAGCCAATAGCTTCTGGTTGCGAACAAACCGAGAATGGTCTTAGAAACAGCGATGCGCTTGCAT
TGATGGCAGCATCGAGTATTAAGCTACCTTACGAGGATATCAATCCTTTTGCTTTTGAGCCTGCGATTGCGCCGCACTTA
GCTGCAGCTGAGAAAGGTGTAAATTTGGCGCCTAATCGTATCGAAGCCCAGTTAAAAATGGCTGAATATGTGGGGGCAGA
TTTTTGCTTAATTGAAGGCGCTGGTGGTTGGCGTCTACCGTTAGGAGAAGGGCATTTCCTTTCTGATGTGGTCCAGTCGA
TGCAGTTACCGGTAATTTTAGTTGTCGGCATGAAATTGGGTTGTTTGAACCATGCGCTATTAACGGCACAAGCGATAAAA
GCCGATGGCTTAAACATTGTAGGCTGGGTAGCTAATCAAGTGGATAAAGATATGGCCTATCAAGAGCAAAACCTAGAGAG
CTTAAAAGCTTTATTGCCAGCGCCTTGTTTAGGCTATGTTCCTTATCTTGCCGAGGCAACAGCCGAAGCTGCGGCTAGCC
ATTTAGATCTAACATTATTAGCCTAA

Upstream 100 bases:

>100_bases
GTTAAATCGAGCCCCAGAGGGCCTACCTTTAACTTATAACATTAGCTTTATAAAGGCGCGTAAGAAGCGCTAAACGCAGC
TGAGTCAACTTGGAGTAGAC

Downstream 100 bases:

>100_bases
TTTCTTTAGCCTTAGTGTAAGCAATTTTATTCGATAAAAAGCAGCCTAATTCAACGGGATATTTTCTGAGTGAATTAGGC
TTATCTTTTTGTTAACAAAG

Product: dethiobiotin synthase

Products: NA

Alternate protein names: DTB synthetase; DTBS; Dethiobiotin synthase [H]

Number of amino acids: Translated: 221; Mature: 220

Protein sequence:

>221_residues
MTFFVTGTDTDCGKTLISSALLAAAKGETVGFKPIASGCEQTENGLRNSDALALMAASSIKLPYEDINPFAFEPAIAPHL
AAAEKGVNLAPNRIEAQLKMAEYVGADFCLIEGAGGWRLPLGEGHFLSDVVQSMQLPVILVVGMKLGCLNHALLTAQAIK
ADGLNIVGWVANQVDKDMAYQEQNLESLKALLPAPCLGYVPYLAEATAEAAASHLDLTLLA

Sequences:

>Translated_221_residues
MTFFVTGTDTDCGKTLISSALLAAAKGETVGFKPIASGCEQTENGLRNSDALALMAASSIKLPYEDINPFAFEPAIAPHL
AAAEKGVNLAPNRIEAQLKMAEYVGADFCLIEGAGGWRLPLGEGHFLSDVVQSMQLPVILVVGMKLGCLNHALLTAQAIK
ADGLNIVGWVANQVDKDMAYQEQNLESLKALLPAPCLGYVPYLAEATAEAAASHLDLTLLA
>Mature_220_residues
TFFVTGTDTDCGKTLISSALLAAAKGETVGFKPIASGCEQTENGLRNSDALALMAASSIKLPYEDINPFAFEPAIAPHLA
AAEKGVNLAPNRIEAQLKMAEYVGADFCLIEGAGGWRLPLGEGHFLSDVVQSMQLPVILVVGMKLGCLNHALLTAQAIKA
DGLNIVGWVANQVDKDMAYQEQNLESLKALLPAPCLGYVPYLAEATAEAAASHLDLTLLA

Specific function: Bioconversion of pimelate into dethiobiotin. [C]

COG id: COG0132

COG function: function code H; Dethiobiotin synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dethiobiotin synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1786995, Length=222, Percent_Identity=48.1981981981982, Blast_Score=201, Evalue=4e-53,
Organism=Escherichia coli, GI87081944, Length=221, Percent_Identity=42.9864253393665, Blast_Score=176, Evalue=1e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004472
- InterPro:   IPR002586 [H]

Pfam domain/function: PF01656 CbiA [H]

EC number: =6.3.3.3 [H]

Molecular weight: Translated: 23242; Mature: 23111

Theoretical pI: Translated: 4.42; Mature: 4.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFFVTGTDTDCGKTLISSALLAAAKGETVGFKPIASGCEQTENGLRNSDALALMAASSI
CEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCC
KLPYEDINPFAFEPAIAPHLAAAEKGVNLAPNRIEAQLKMAEYVGADFCLIEGAGGWRLP
CCCHHHCCCCEECCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCEECC
LGEGHFLSDVVQSMQLPVILVVGMKLGCLNHALLTAQAIKADGLNIVGWVANQVDKDMAY
CCCCHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHH
QEQNLESLKALLPAPCLGYVPYLAEATAEAAASHLDLTLLA
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCEEEEC
>Mature Secondary Structure 
TFFVTGTDTDCGKTLISSALLAAAKGETVGFKPIASGCEQTENGLRNSDALALMAASSI
EEEEECCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCC
KLPYEDINPFAFEPAIAPHLAAAEKGVNLAPNRIEAQLKMAEYVGADFCLIEGAGGWRLP
CCCHHHCCCCEECCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCEECC
LGEGHFLSDVVQSMQLPVILVVGMKLGCLNHALLTAQAIKADGLNIVGWVANQVDKDMAY
CCCCHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHH
QEQNLESLKALLPAPCLGYVPYLAEATAEAAASHLDLTLLA
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA