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The map label for this gene is sacIM [H]

Identifier: 157961098

GI number: 157961098

Start: 1546309

End: 1547529

Strand: Direct

Name: sacIM [H]

Synonym: Spea_1270

Alternate gene names: 157961098

Gene position: 1546309-1547529 (Clockwise)

Preceding gene: 157961097

Following gene: 157961099

Centisome position: 29.88

GC content: 42.42

Gene sequence:

>1221_bases
ATGATCTCGCTTTTTAGTGGCGCTGGTGGACTAGATATTGGTTTATGTGAGGCCGGGTTTACTAATAGGCTCAGTGTAGA
AATTGATGAAGATGCACAAAAAACACTTAAATTGAACCAACCATCGTTGAAGTTGGCAACACCAGGAGATATTCATAAAT
TAACTCCTAGTGAGTTACTGAGACAGTCAGGATTACAGCCTAAAGAGCTTACTTTATTAGCTGGTGGACCTCCATGCCAA
CCATTTTCTAAGTCTGGTTACTGGGCTAAAGGGGATACTGACCGATTAACAGACCCTAGGGCTGAAACACTAACTGCATA
CCTTGATATCGTGAAGGTGGCATTGCCTCAAGTGCTTCTGTTAGAAAATGTAAAAGGCATTGCTTATTCAGATAAAGATG
AAGGTTTACAGCTACTCATTACAGAGCTAAACAACATAAATAGTGAAATGAATACTAGCTATATGCCTGAAGTCATCACT
GTCAATGCTTCAAGTTATGGTGTTCCACAACATAGAGAACGAGTATTCATTGTTGCTAATAGGGATGGAGGTACTTTCAA
GATGCCTTCGCCTACTCATAGTGACAGTAAAGATCCAACCTTGAAAAATACTTTGCGGTGTACAACTGCTTGGGATTCTA
TTGGTGACTTGGATGTTGAACCGTGGCCTGAAGATATCAAACCTAAAGGAAAATGGGCCGATCTATTACCTACCATTCCT
GAAGGGCAAAATTATCTTTGGCATACGGATCGATTTGGGGGCAGTCCGTTGTTTGGTTGGCGAACTCGGTTCTGGTCATT
TTTACTAAAGCTAGCAAAAAATCAACCAAGTTGGACCATTCAGGCACAGCCAGGTCCTGCTACGGGACCATTTCACTGGA
AAGGTCGTTTATTGTCTATTAGGGAATTAGCCCGTTTACAGACATTTCCCGATTCGTACCAGTTTGTAGGTGATCGACGC
TCTGCTCAGAAACAGATTGGAAATGCTGTCCCACCAGCAATGGGTGAGTTATTTGGCTTAGAAATCAGGCGCCAGTTTTT
TGGTGAACAAGTACGTAGAAAACTCAGATTAATTCCTGAGGGAAGAGAAGATTGTCCTGTCCCTGAAGTTCCCAAACAGG
TGCCTGAAAAGTTCTTAGAAATGGTAGGTAGTCATAAGGCCCATCCCGGAACAGGAAAGGGACCTGGAGCGGTTTTAAGA
AATACAAATAACGTAGTCTAG

Upstream 100 bases:

>100_bases
GTTGTGAAGCACTAAAATAACCCCCGATAGTAATTTAGTCAGAGTCTTTGAGGTGATAGTTTGAGTGGTGCCGTAAGTGC
TTAATAATAGAAAGAAAACA

Downstream 100 bases:

>100_bases
ATTTATAGTCCGAAACCTTTCTAATTAAGGAACTGTGAGTGACATCGCACATAATTATTACAACAGGAACATTACTTCGT
GAACTCTCTCGCTTCCGACT

Product: DNA-cytosine methyltransferase

Products: NA

Alternate protein names: M.SacI; Cytosine-specific methyltransferase SacI [H]

Number of amino acids: Translated: 406; Mature: 406

Protein sequence:

>406_residues
MISLFSGAGGLDIGLCEAGFTNRLSVEIDEDAQKTLKLNQPSLKLATPGDIHKLTPSELLRQSGLQPKELTLLAGGPPCQ
PFSKSGYWAKGDTDRLTDPRAETLTAYLDIVKVALPQVLLLENVKGIAYSDKDEGLQLLITELNNINSEMNTSYMPEVIT
VNASSYGVPQHRERVFIVANRDGGTFKMPSPTHSDSKDPTLKNTLRCTTAWDSIGDLDVEPWPEDIKPKGKWADLLPTIP
EGQNYLWHTDRFGGSPLFGWRTRFWSFLLKLAKNQPSWTIQAQPGPATGPFHWKGRLLSIRELARLQTFPDSYQFVGDRR
SAQKQIGNAVPPAMGELFGLEIRRQFFGEQVRRKLRLIPEGREDCPVPEVPKQVPEKFLEMVGSHKAHPGTGKGPGAVLR
NTNNVV

Sequences:

>Translated_406_residues
MISLFSGAGGLDIGLCEAGFTNRLSVEIDEDAQKTLKLNQPSLKLATPGDIHKLTPSELLRQSGLQPKELTLLAGGPPCQ
PFSKSGYWAKGDTDRLTDPRAETLTAYLDIVKVALPQVLLLENVKGIAYSDKDEGLQLLITELNNINSEMNTSYMPEVIT
VNASSYGVPQHRERVFIVANRDGGTFKMPSPTHSDSKDPTLKNTLRCTTAWDSIGDLDVEPWPEDIKPKGKWADLLPTIP
EGQNYLWHTDRFGGSPLFGWRTRFWSFLLKLAKNQPSWTIQAQPGPATGPFHWKGRLLSIRELARLQTFPDSYQFVGDRR
SAQKQIGNAVPPAMGELFGLEIRRQFFGEQVRRKLRLIPEGREDCPVPEVPKQVPEKFLEMVGSHKAHPGTGKGPGAVLR
NTNNVV
>Mature_406_residues
MISLFSGAGGLDIGLCEAGFTNRLSVEIDEDAQKTLKLNQPSLKLATPGDIHKLTPSELLRQSGLQPKELTLLAGGPPCQ
PFSKSGYWAKGDTDRLTDPRAETLTAYLDIVKVALPQVLLLENVKGIAYSDKDEGLQLLITELNNINSEMNTSYMPEVIT
VNASSYGVPQHRERVFIVANRDGGTFKMPSPTHSDSKDPTLKNTLRCTTAWDSIGDLDVEPWPEDIKPKGKWADLLPTIP
EGQNYLWHTDRFGGSPLFGWRTRFWSFLLKLAKNQPSWTIQAQPGPATGPFHWKGRLLSIRELARLQTFPDSYQFVGDRR
SAQKQIGNAVPPAMGELFGLEIRRQFFGEQVRRKLRLIPEGREDCPVPEVPKQVPEKFLEMVGSHKAHPGTGKGPGAVLR
NTNNVV

Specific function: This methylase recognizes the double-stranded sequence GAGCTC, causes specific methylation on C-4 on both strands, and protects the DNA from cleavage by the SacI endonuclease [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001525 [H]

Pfam domain/function: PF00145 DNA_methylase [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 45072; Mature: 45072

Theoretical pI: Translated: 8.31; Mature: 8.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISLFSGAGGLDIGLCEAGFTNRLSVEIDEDAQKTLKLNQPSLKLATPGDIHKLTPSELL
CEEEECCCCCCEEEEECCCCCCEEEEEECCCHHHHEECCCCCEEECCCCCCCCCCHHHHH
RQSGLQPKELTLLAGGPPCQPFSKSGYWAKGDTDRLTDPRAETLTAYLDIVKVALPQVLL
HHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LENVKGIAYSDKDEGLQLLITELNNINSEMNTSYMPEVITVNASSYGVPQHRERVFIVAN
HHCCCCCEECCCCCCHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCCEEEEEEE
RDGGTFKMPSPTHSDSKDPTLKNTLRCTTAWDSIGDLDVEPWPEDIKPKGKWADLLPTIP
CCCCEEECCCCCCCCCCCCCHHHHEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHCCCCC
EGQNYLWHTDRFGGSPLFGWRTRFWSFLLKLAKNQPSWTIQAQPGPATGPFHWKGRLLSI
CCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCHHHHH
RELARLQTFPDSYQFVGDRRSAQKQIGNAVPPAMGELFGLEIRRQFFGEQVRRKLRLIPE
HHHHHHHCCCCHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GREDCPVPEVPKQVPEKFLEMVGSHKAHPGTGKGPGAVLRNTNNVV
CCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCEECCCCCCC
>Mature Secondary Structure
MISLFSGAGGLDIGLCEAGFTNRLSVEIDEDAQKTLKLNQPSLKLATPGDIHKLTPSELL
CEEEECCCCCCEEEEECCCCCCEEEEEECCCHHHHEECCCCCEEECCCCCCCCCCHHHHH
RQSGLQPKELTLLAGGPPCQPFSKSGYWAKGDTDRLTDPRAETLTAYLDIVKVALPQVLL
HHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LENVKGIAYSDKDEGLQLLITELNNINSEMNTSYMPEVITVNASSYGVPQHRERVFIVAN
HHCCCCCEECCCCCCHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCCEEEEEEE
RDGGTFKMPSPTHSDSKDPTLKNTLRCTTAWDSIGDLDVEPWPEDIKPKGKWADLLPTIP
CCCCEEECCCCCCCCCCCCCHHHHEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHCCCCC
EGQNYLWHTDRFGGSPLFGWRTRFWSFLLKLAKNQPSWTIQAQPGPATGPFHWKGRLLSI
CCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCHHHHH
RELARLQTFPDSYQFVGDRRSAQKQIGNAVPPAMGELFGLEIRRQFFGEQVRRKLRLIPE
HHHHHHHCCCCHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GREDCPVPEVPKQVPEKFLEMVGSHKAHPGTGKGPGAVLRNTNNVV
CCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9862476 [H]