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The map label for this gene is nrfA [H]

Identifier: 157961056

GI number: 157961056

Start: 1493670

End: 1495079

Strand: Direct

Name: nrfA [H]

Synonym: Spea_1228

Alternate gene names: 157961056

Gene position: 1493670-1495079 (Clockwise)

Preceding gene: 157961055

Following gene: 157961057

Centisome position: 28.87

GC content: 48.87

Gene sequence:

>1410_bases
ATGACAGTCATAAATCTGATGAAAGTTGCTGGTGCGTTCTCTCTAGCTTTGCTGATGCCGATTACGGCTGTACAGGCAGA
AGAAAACGCCAATGCACAGACAGCGGCAGCAAAAGAGCAGCAGCTTAGAGATAAATCGCTGCGTGAAAAGTTTCCAGTGC
AGTATCAAAGCTGGCAGGAGACCTCTGAGCAGAGCGTGCGTGAAGATATGCTGGCCAGCTATCCTGCGGCGATTATTTTG
TGGGCGGGTTCGTCATTTGCCAAAGAGTACAATAGCCCTCGCGGCCATCACTTCGCGGTGGCAGATGTGACTCACACGCT
GCGTACTGGGGTGCCGCCAGCGGCAGGAGATAAGGGCTTGTCTGGTAGCTGCTGGACCTGTAAAACCCCCGATGCGCCAA
GACTGATGAAAGAGTTAGGTGTTGAAGGTTTTTCTGCCAAAAACTTTACCGATGTTGGCACTGAAGTTAATAGTGTGGTC
TATTGCACCGATTGTCACGTTGACGGCAAGGCCGAGCTGGCTCTGCCACGGCCGCATGCCCAAGATGCGATGGCAAAAGT
GCACCTGCCTTTCGATAAGCAAGATGCCAATATGCAAGGAGCACAGGTTTGTGGTCAGTGTCATGTGACTTACTATTTCC
AACCCGAGCGCAGTGACAAGGTCAATATACCGTGGATCTTCGGTAACAATGTTGACGATATAGAGAAGTATTACGATACC
CGTCGCTTCTATGAGTGGATCCATCCTATTTCGAAAACACCCATCTTAAAGGCGCGACACCCTGAGTTTGAGCACTGGGT
GAGAAGTGAGCACGCCGAGGCTGGGGTAACCTGTATTACCTGCCATATGCCGGAAGAAACAGATAAGCAAGGCAATAGCT
TTACCAGCCATAAAGTGAGTAAAGCACTGGATCATTTCGATTCGGTTTGCCAAAACTGTCACAAGAGCGAAAAAACCATT
ATCAAGTCCCTTGAAAACAGCAAAGCTAAAATTGATGCTAAAGCGCGAGAAGTGGAAGGACTGCTAGTTAAAGCACACTA
CGAGGCGCAAGCAGCGTGGGAGGCGGGCGCAACATGGCCGTTGATGAACGATGCCATTATGGCTATTCGTCATTCACAGT
GGCGCTGGGATTTTGCCATGGCGTCCCATGGCCTGTACGCCCATAACCCCAAAGAAGGCAATGCGCTTTTAGATATGTCG
ATCAGACAGGTTAATTATGCCCGTGAGTCATTAGCCGAGATCTTGAAGAAGTTTGCTGTTAGCAAAGTGGACTATCCCGA
CATTAGCAGCAAAGAGTCGGCTCAAGCTGCTGTTGGCATTAAGCTTGATAAATTGACCCAAGAAAAGCAGGAATTTATTA
AGCAAGAGGTTGATAAAAATTGGTCTGAGGTGACTCGCTACGGTTACTAA

Upstream 100 bases:

>100_bases
CAGGATAGTTTTCATCCTTAATATGAAGACAGATAGAAAATAGAAAAAAAACAGTTAGATAAATGCTAAATAAAAAGATG
ATAAGGTTGATAAAAAACAG

Downstream 100 bases:

>100_bases
TCGTCTTGCAGCATGACTTAAGTTAGCGGCTTAAGTTAGCGGCTTAAGTTAGTGGCTTAAGTTAAAGGCTCTAAGGTCCT
CCTTTGAGCTTTTTTCTTTA

Product: nitrite reductase

Products: NA

Alternate protein names: Ammonia-forming cytochrome c nitrite reductase; Cytochrome c nitrite reductase [H]

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MTVINLMKVAGAFSLALLMPITAVQAEENANAQTAAAKEQQLRDKSLREKFPVQYQSWQETSEQSVREDMLASYPAAIIL
WAGSSFAKEYNSPRGHHFAVADVTHTLRTGVPPAAGDKGLSGSCWTCKTPDAPRLMKELGVEGFSAKNFTDVGTEVNSVV
YCTDCHVDGKAELALPRPHAQDAMAKVHLPFDKQDANMQGAQVCGQCHVTYYFQPERSDKVNIPWIFGNNVDDIEKYYDT
RRFYEWIHPISKTPILKARHPEFEHWVRSEHAEAGVTCITCHMPEETDKQGNSFTSHKVSKALDHFDSVCQNCHKSEKTI
IKSLENSKAKIDAKAREVEGLLVKAHYEAQAAWEAGATWPLMNDAIMAIRHSQWRWDFAMASHGLYAHNPKEGNALLDMS
IRQVNYARESLAEILKKFAVSKVDYPDISSKESAQAAVGIKLDKLTQEKQEFIKQEVDKNWSEVTRYGY

Sequences:

>Translated_469_residues
MTVINLMKVAGAFSLALLMPITAVQAEENANAQTAAAKEQQLRDKSLREKFPVQYQSWQETSEQSVREDMLASYPAAIIL
WAGSSFAKEYNSPRGHHFAVADVTHTLRTGVPPAAGDKGLSGSCWTCKTPDAPRLMKELGVEGFSAKNFTDVGTEVNSVV
YCTDCHVDGKAELALPRPHAQDAMAKVHLPFDKQDANMQGAQVCGQCHVTYYFQPERSDKVNIPWIFGNNVDDIEKYYDT
RRFYEWIHPISKTPILKARHPEFEHWVRSEHAEAGVTCITCHMPEETDKQGNSFTSHKVSKALDHFDSVCQNCHKSEKTI
IKSLENSKAKIDAKAREVEGLLVKAHYEAQAAWEAGATWPLMNDAIMAIRHSQWRWDFAMASHGLYAHNPKEGNALLDMS
IRQVNYARESLAEILKKFAVSKVDYPDISSKESAQAAVGIKLDKLTQEKQEFIKQEVDKNWSEVTRYGY
>Mature_468_residues
TVINLMKVAGAFSLALLMPITAVQAEENANAQTAAAKEQQLRDKSLREKFPVQYQSWQETSEQSVREDMLASYPAAIILW
AGSSFAKEYNSPRGHHFAVADVTHTLRTGVPPAAGDKGLSGSCWTCKTPDAPRLMKELGVEGFSAKNFTDVGTEVNSVVY
CTDCHVDGKAELALPRPHAQDAMAKVHLPFDKQDANMQGAQVCGQCHVTYYFQPERSDKVNIPWIFGNNVDDIEKYYDTR
RFYEWIHPISKTPILKARHPEFEHWVRSEHAEAGVTCITCHMPEETDKQGNSFTSHKVSKALDHFDSVCQNCHKSEKTII
KSLENSKAKIDAKAREVEGLLVKAHYEAQAAWEAGATWPLMNDAIMAIRHSQWRWDFAMASHGLYAHNPKEGNALLDMSI
RQVNYARESLAEILKKFAVSKVDYPDISSKESAQAAVGIKLDKLTQEKQEFIKQEVDKNWSEVTRYGY

Specific function: Plays a role in nitrite reduction [H]

COG id: COG3303

COG function: function code P; Formate-dependent nitrite reductase, periplasmic cytochrome c552 subunit

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cytochrome c-552 family [H]

Homologues:

Organism=Escherichia coli, GI1790506, Length=475, Percent_Identity=40.8421052631579, Blast_Score=367, Evalue=1e-102,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003321
- InterPro:   IPR017570
- InterPro:   IPR011031 [H]

Pfam domain/function: PF02335 Cytochrom_C552 [H]

EC number: =1.7.2.2 [H]

Molecular weight: Translated: 52713; Mature: 52582

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: PS51008 MULTIHEME_CYTC ; PS01047 HMA_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVINLMKVAGAFSLALLMPITAVQAEENANAQTAAAKEQQLRDKSLREKFPVQYQSWQE
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHH
TSEQSVREDMLASYPAAIILWAGSSFAKEYNSPRGHHFAVADVTHTLRTGVPPAAGDKGL
HHHHHHHHHHHHHCCCEEEEECCCHHHHHCCCCCCCEEEHHHHHHHHHCCCCCCCCCCCC
SGSCWTCKTPDAPRLMKELGVEGFSAKNFTDVGTEVNSVVYCTDCHVDGKAELALPRPHA
CCCCEECCCCCHHHHHHHHCCCCCCCCCCHHCCCCCCCEEEEEECCCCCCCEEECCCCCH
QDAMAKVHLPFDKQDANMQGAQVCGQCHVTYYFQPERSDKVNIPWIFGNNVDDIEKYYDT
HHHHEEEECCCCCCCCCCCHHHHCCEEEEEEEECCCCCCCEEEEEEECCCHHHHHHHHHH
RRFYEWIHPISKTPILKARHPEFEHWVRSEHAEAGVTCITCHMPEETDKQGNSFTSHKVS
HHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCHHHHHHH
KALDHFDSVCQNCHKSEKTIIKSLENSKAKIDAKAREVEGLLVKAHYEAQAAWEAGATWP
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCEEEEEEECHHHHHHCCCCCCC
LMNDAIMAIRHSQWRWDFAMASHGLYAHNPKEGNALLDMSIRQVNYARESLAEILKKFAV
CHHHHHHHHHCCCCEEEHHHHCCCCEECCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHH
SKVDYPDISSKESAQAAVGIKLDKLTQEKQEFIKQEVDKNWSEVTRYGY
CCCCCCCCCCCCCCHHHHCEEHHHHHHHHHHHHHHHHCCCHHHHHHCCC
>Mature Secondary Structure 
TVINLMKVAGAFSLALLMPITAVQAEENANAQTAAAKEQQLRDKSLREKFPVQYQSWQE
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHH
TSEQSVREDMLASYPAAIILWAGSSFAKEYNSPRGHHFAVADVTHTLRTGVPPAAGDKGL
HHHHHHHHHHHHHCCCEEEEECCCHHHHHCCCCCCCEEEHHHHHHHHHCCCCCCCCCCCC
SGSCWTCKTPDAPRLMKELGVEGFSAKNFTDVGTEVNSVVYCTDCHVDGKAELALPRPHA
CCCCEECCCCCHHHHHHHHCCCCCCCCCCHHCCCCCCCEEEEEECCCCCCCEEECCCCCH
QDAMAKVHLPFDKQDANMQGAQVCGQCHVTYYFQPERSDKVNIPWIFGNNVDDIEKYYDT
HHHHEEEECCCCCCCCCCCHHHHCCEEEEEEEECCCCCCCEEEEEEECCCHHHHHHHHHH
RRFYEWIHPISKTPILKARHPEFEHWVRSEHAEAGVTCITCHMPEETDKQGNSFTSHKVS
HHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCHHHHHHH
KALDHFDSVCQNCHKSEKTIIKSLENSKAKIDAKAREVEGLLVKAHYEAQAAWEAGATWP
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCEEEEEEECHHHHHHCCCCCCC
LMNDAIMAIRHSQWRWDFAMASHGLYAHNPKEGNALLDMSIRQVNYARESLAEILKKFAV
CHHHHHHHHHCCCCEEEHHHHCCCCEECCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHH
SKVDYPDISSKESAQAAVGIKLDKLTQEKQEFIKQEVDKNWSEVTRYGY
CCCCCCCCCCCCCCHHHHCEEHHHHHHHHHHHHHHHHCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA