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The map label for this gene is bepF [H]

Identifier: 157960978

GI number: 157960978

Start: 1388659

End: 1389813

Strand: Direct

Name: bepF [H]

Synonym: Spea_1150

Alternate gene names: 157960978

Gene position: 1388659-1389813 (Clockwise)

Preceding gene: 157960970

Following gene: 157960979

Centisome position: 26.84

GC content: 47.1

Gene sequence:

>1155_bases
ATGGCCGGTTCATCAGTTTCAAAAACTAAGTTGTGGATGTTAGTTATTAGCGCCCTCGCAATATCTGCCTGTGGAGACGC
CCCTGCGCCACAACAAGCAGTGATTCCAAGTGTCATCGTTAATACCGCTCACAAAGAAATGATCACGCCTAAGTCTGAAT
TTGTCGGCCGAACCAGAGCATCTGAAGACGTGGTGATTAAACCGCAGATCCAGGGCCAGTTATTACGACGTAGTTATATT
GAAGGCGATGATATTGCCGCAGGCGATCTCTTGTTTGAAATCGACCCATCGACCTATAAAGCGGAGCTTGCTCAGCATCA
AGCCGTATTAAAGCAGGCGGTTGCATCGAGGGACGTGGCGGTAATGAACTGGGAACGTGGTCGCCGGTTACTGCCCGATG
GCATGATCAGTGCGCAGGATATGGATGAATTGACCAGCCGTAAGCTCACGACTGCTGCAGGCGTTGTGCAAGCCGAAGCG
GCAGTAGATGCTGCAGAGTTGCAATTAAGTTATACCAAGGTTTACGCGCCTATTTCTGGCCGTATAAGTCATTCTAAAGT
CAGTATTGGCGACATCATTACTCCCCAGTCTGAAATGGCTAATATCGTGCAGCTCCAGCCGATGTGGGTCAATTTTCAAG
TGGCAGAGAAAGGGCTTATCACGGCACAACAGCAATTTTCTCAGGCTAAGAATATCGATATGAAGATTGAAGATCTTGTT
ATGAGGCTGCGCCTGCCTAACGGCACCATGTTTGATGAAACAGGTCATATCGACTTTATTGGTAACCGAGTCGACGCAAC
GACAGGTACCTTAGCTGTACGTGCCACATTTAAAAATGCCAATAGCTTAATGTTGCCGGGCCTTTTTGTAACCTTAGTGG
TTGAATCACCAATCACTGAAAATGCGCTACTTATCCCTCAGGCTTCGGTTCAGGAAGATCAACAAGGTCGTTTTGTGATG
GTGTTAAACGACCAAGATGAAGTACAAAAACGCCTAGTTGTACTAGGCGACCGTTTCGGTGTCGAGTGGCGTGTACTCAG
TGGCCTAGAAGATGGTGATCGTATCGTGGTCGAGGGATTACAGAAAATTCGCCCAGGTATGAAAGTGAACCCTGTTGAAC
AAGAAGTTGTTCCCTTTAAAGAAGATGACAAATAA

Upstream 100 bases:

>100_bases
GTTTTGCCTCTTTGTTACCTAAAGGTTTTATTTATAGGTGAGTTAAGCTTATAATTTCAGAACAAAAATAAAGCACATAT
CCTACCTACTGGAGGCTTAA

Downstream 100 bases:

>100_bases
AACAGGAGCGAGTAATGAGACATCAATGCTTAATTGCTAACTTCGTAACTTGTTGCATCGATAGTTGCCTAAGCCTCTAT
ATTAAGGTCGACAGGGGAAT

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 384; Mature: 383

Protein sequence:

>384_residues
MAGSSVSKTKLWMLVISALAISACGDAPAPQQAVIPSVIVNTAHKEMITPKSEFVGRTRASEDVVIKPQIQGQLLRRSYI
EGDDIAAGDLLFEIDPSTYKAELAQHQAVLKQAVASRDVAVMNWERGRRLLPDGMISAQDMDELTSRKLTTAAGVVQAEA
AVDAAELQLSYTKVYAPISGRISHSKVSIGDIITPQSEMANIVQLQPMWVNFQVAEKGLITAQQQFSQAKNIDMKIEDLV
MRLRLPNGTMFDETGHIDFIGNRVDATTGTLAVRATFKNANSLMLPGLFVTLVVESPITENALLIPQASVQEDQQGRFVM
VLNDQDEVQKRLVVLGDRFGVEWRVLSGLEDGDRIVVEGLQKIRPGMKVNPVEQEVVPFKEDDK

Sequences:

>Translated_384_residues
MAGSSVSKTKLWMLVISALAISACGDAPAPQQAVIPSVIVNTAHKEMITPKSEFVGRTRASEDVVIKPQIQGQLLRRSYI
EGDDIAAGDLLFEIDPSTYKAELAQHQAVLKQAVASRDVAVMNWERGRRLLPDGMISAQDMDELTSRKLTTAAGVVQAEA
AVDAAELQLSYTKVYAPISGRISHSKVSIGDIITPQSEMANIVQLQPMWVNFQVAEKGLITAQQQFSQAKNIDMKIEDLV
MRLRLPNGTMFDETGHIDFIGNRVDATTGTLAVRATFKNANSLMLPGLFVTLVVESPITENALLIPQASVQEDQQGRFVM
VLNDQDEVQKRLVVLGDRFGVEWRVLSGLEDGDRIVVEGLQKIRPGMKVNPVEQEVVPFKEDDK
>Mature_383_residues
AGSSVSKTKLWMLVISALAISACGDAPAPQQAVIPSVIVNTAHKEMITPKSEFVGRTRASEDVVIKPQIQGQLLRRSYIE
GDDIAAGDLLFEIDPSTYKAELAQHQAVLKQAVASRDVAVMNWERGRRLLPDGMISAQDMDELTSRKLTTAAGVVQAEAA
VDAAELQLSYTKVYAPISGRISHSKVSIGDIITPQSEMANIVQLQPMWVNFQVAEKGLITAQQQFSQAKNIDMKIEDLVM
RLRLPNGTMFDETGHIDFIGNRVDATTGTLAVRATFKNANSLMLPGLFVTLVVESPITENALLIPQASVQEDQQGRFVMV
LNDQDEVQKRLVVLGDRFGVEWRVLSGLEDGDRIVVEGLQKIRPGMKVNPVEQEVVPFKEDDK

Specific function: May contribute to resistance to some drugs, such as deoxycholate, sodium dodecyl sulfate and nalidixic acid, in the absence of BepD and BepE [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789929, Length=362, Percent_Identity=33.1491712707182, Blast_Score=201, Evalue=8e-53,
Organism=Escherichia coli, GI1786668, Length=367, Percent_Identity=34.3324250681199, Blast_Score=190, Evalue=1e-49,
Organism=Escherichia coli, GI1789665, Length=361, Percent_Identity=33.2409972299169, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI87082036, Length=330, Percent_Identity=30.6060606060606, Blast_Score=157, Evalue=9e-40,
Organism=Escherichia coli, GI87081796, Length=319, Percent_Identity=23.8244514106583, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI87081951, Length=137, Percent_Identity=33.5766423357664, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 42119; Mature: 41988

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGSSVSKTKLWMLVISALAISACGDAPAPQQAVIPSVIVNTAHKEMITPKSEFVGRTRA
CCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHCCCCC
SEDVVIKPQIQGQLLRRSYIEGDDIAAGDLLFEIDPSTYKAELAQHQAVLKQAVASRDVA
CCCEEECCCHHHHHHHHHCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCEE
VMNWERGRRLLPDGMISAQDMDELTSRKLTTAAGVVQAEAAVDAAELQLSYTKVYAPISG
EEEHHCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEEEEEEEEECCCC
RISHSKVSIGDIITPQSEMANIVQLQPMWVNFQVAEKGLITAQQQFSQAKNIDMKIEDLV
CCCCCEEECCCCCCCHHHHHHEEEECEEEEEEEECCCCHHHHHHHHHHHCCCCCCHHHEE
MRLRLPNGTMFDETGHIDFIGNRVDATTGTLAVRATFKNANSLMLPGLFVTLVVESPITE
HEEECCCCCEECCCCCEEEECCEECCCCCEEEEEEEECCCCCEECCCEEEEEEECCCCCC
NALLIPQASVQEDQQGRFVMVLNDQDEVQKRLVVLGDRFGVEWRVLSGLEDGDRIVVEGL
CEEEECCCCCCCCCCCCEEEEECCHHHHHHHHEEECCCCCCEEEEECCCCCCCHHHHHHH
QKIRPGMKVNPVEQEVVPFKEDDK
HHHCCCCCCCCCHHHCCCCCCCCC
>Mature Secondary Structure 
AGSSVSKTKLWMLVISALAISACGDAPAPQQAVIPSVIVNTAHKEMITPKSEFVGRTRA
CCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHCCCCC
SEDVVIKPQIQGQLLRRSYIEGDDIAAGDLLFEIDPSTYKAELAQHQAVLKQAVASRDVA
CCCEEECCCHHHHHHHHHCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCEE
VMNWERGRRLLPDGMISAQDMDELTSRKLTTAAGVVQAEAAVDAAELQLSYTKVYAPISG
EEEHHCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEEEEEEEEECCCC
RISHSKVSIGDIITPQSEMANIVQLQPMWVNFQVAEKGLITAQQQFSQAKNIDMKIEDLV
CCCCCEEECCCCCCCHHHHHHEEEECEEEEEEEECCCCHHHHHHHHHHHCCCCCCHHHEE
MRLRLPNGTMFDETGHIDFIGNRVDATTGTLAVRATFKNANSLMLPGLFVTLVVESPITE
HEEECCCCCEECCCCCEEEECCEECCCCCEEEEEEEECCCCCEECCCEEEEEEECCCCCC
NALLIPQASVQEDQQGRFVMVLNDQDEVQKRLVVLGDRFGVEWRVLSGLEDGDRIVVEGL
CEEEECCCCCCCCCCCCEEEEECCHHHHHHHHEEECCCCCCEEEEECCCCCCCHHHHHHH
QKIRPGMKVNPVEQEVVPFKEDDK
HHHCCCCCCCCCHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12271122 [H]