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The map label for this gene is mscS [H]

Identifier: 157960968

GI number: 157960968

Start: 1376318

End: 1377142

Strand: Direct

Name: mscS [H]

Synonym: Spea_1140

Alternate gene names: 157960968

Gene position: 1376318-1377142 (Clockwise)

Preceding gene: 157960967

Following gene: 157960970

Centisome position: 26.6

GC content: 45.45

Gene sequence:

>825_bases
ATGGATAACTTGCAAGGGTTAATGGAGCAAGCGCCTGAACTGATCGTCACCTACGGTATGAAAGTGCTGTTCGCGATTAT
CATTTTTGTCATAGGTAAATTCCTATCGAATGTTGCCAAGAATCTAACATCTAAGCTGCTGAAAAAGCGTAAAGTCGACA
ACACAGTTGTGTCGTTTGTTGCCAATATGGCTTGGGCATTAGTGTTCGTATTTACCATAGTTGCTACGCTAGGGCAGATA
GGTGTGCAAACCGCTTCGTTAGTTGCTGTGATTGGTGCCGCCGGTTTAGCTGTTGGTTTAGCACTGCAAGGCTCTTTGTC
TAACTTTGCTGCCGGGGTATTGATGGTACTCTTTAGGCCGTGTCGAGTTGGTGACTATGTGGAAGCGGCGGGCGTTGCTG
GTACTGTCGATGAGATCACTATCTTTTCAACCCGATTACTGACTCCTGATAATAAGGTTATTATTGCGCCAAATTCAGCC
ATGATGAACGGCACTATAGTTAACTACTCTGCGATGGACACTCGTCGTTTAGATTTAGTTATTGGGGTTTCTTATGACGC
CAATCTTGCCGAGACCAAAAAGGTATTAACTGACATTCTTGAAAACAGTCAGTATGTGCTCAAAGATCCTGCCTACACAG
TGGCTGTGGCAGAGTTAGCCGACTCATCGGTTAACTTCGTGGTTCGCCCCTGGGTTAAAGGCTCTGACTACTGGCCTGCA
CATTTTGAAATCTTAGAGCAGATAAAGGTCGCTCTAGACGAAGCTGGCATAGGTATTCCGTATCCACAAATGGATCTCTA
TGTAAAAGAGACCCCTGCGGCTTAA

Upstream 100 bases:

>100_bases
TTGCTAATAATCAAACCGCGTTCGGCCGTGAACAGAATCGACGCGTTGAGTTTTTAGTTATTGTTCAATAGTTATTTCTT
AAATTTGAGGGAGTATATAA

Downstream 100 bases:

>100_bases
ACAAAGCCAGTAGATATTGGATAATAAAAAGGTCGGCATATGCCGACCTTTTTATTAGAATTTCTTATTAAACCTAATGG
GCGTTATCAGACTGATACTG

Product: mechanosensitive ion channel protein MscS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MDNLQGLMEQAPELIVTYGMKVLFAIIIFVIGKFLSNVAKNLTSKLLKKRKVDNTVVSFVANMAWALVFVFTIVATLGQI
GVQTASLVAVIGAAGLAVGLALQGSLSNFAAGVLMVLFRPCRVGDYVEAAGVAGTVDEITIFSTRLLTPDNKVIIAPNSA
MMNGTIVNYSAMDTRRLDLVIGVSYDANLAETKKVLTDILENSQYVLKDPAYTVAVAELADSSVNFVVRPWVKGSDYWPA
HFEILEQIKVALDEAGIGIPYPQMDLYVKETPAA

Sequences:

>Translated_274_residues
MDNLQGLMEQAPELIVTYGMKVLFAIIIFVIGKFLSNVAKNLTSKLLKKRKVDNTVVSFVANMAWALVFVFTIVATLGQI
GVQTASLVAVIGAAGLAVGLALQGSLSNFAAGVLMVLFRPCRVGDYVEAAGVAGTVDEITIFSTRLLTPDNKVIIAPNSA
MMNGTIVNYSAMDTRRLDLVIGVSYDANLAETKKVLTDILENSQYVLKDPAYTVAVAELADSSVNFVVRPWVKGSDYWPA
HFEILEQIKVALDEAGIGIPYPQMDLYVKETPAA
>Mature_274_residues
MDNLQGLMEQAPELIVTYGMKVLFAIIIFVIGKFLSNVAKNLTSKLLKKRKVDNTVVSFVANMAWALVFVFTIVATLGQI
GVQTASLVAVIGAAGLAVGLALQGSLSNFAAGVLMVLFRPCRVGDYVEAAGVAGTVDEITIFSTRLLTPDNKVIIAPNSA
MMNGTIVNYSAMDTRRLDLVIGVSYDANLAETKKVLTDILENSQYVLKDPAYTVAVAELADSSVNFVVRPWVKGSDYWPA
HFEILEQIKVALDEAGIGIPYPQMDLYVKETPAA

Specific function: Mechanosensitive channel that participates in the regulation of osmotic pressure changes within the cell. Forms an ion channel of 1.0 nanosiemens conductance with a slight preference for anions. The channel is sensitive to voltage [H]

COG id: COG0668

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1789291, Length=264, Percent_Identity=46.2121212121212, Blast_Score=249, Evalue=1e-67,
Organism=Escherichia coli, GI2367355, Length=268, Percent_Identity=26.4925373134328, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1786670, Length=195, Percent_Identity=28.2051282051282, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1787591, Length=202, Percent_Identity=27.7227722772277, Blast_Score=82, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014
- InterPro:   IPR008910 [H]

Pfam domain/function: PF00924 MS_channel; PF05552 TM_helix [H]

EC number: NA

Molecular weight: Translated: 29524; Mature: 29524

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNLQGLMEQAPELIVTYGMKVLFAIIIFVIGKFLSNVAKNLTSKLLKKRKVDNTVVSFV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ANMAWALVFVFTIVATLGQIGVQTASLVAVIGAAGLAVGLALQGSLSNFAAGVLMVLFRP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHC
CRVGDYVEAAGVAGTVDEITIFSTRLLTPDNKVIIAPNSAMMNGTIVNYSAMDTRRLDLV
CCCCCHHHHCCCCCCHHHHEEEHEEEECCCCEEEECCCCCEECCEEEEEECCCCEEEEEE
IGVSYDANLAETKKVLTDILENSQYVLKDPAYTVAVAELADSSVNFVVRPWVKGSDYWPA
EEECCCCCHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHCCCCCCEEEECCCCCCCCCCH
HFEILEQIKVALDEAGIGIPYPQMDLYVKETPAA
HHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCC
>Mature Secondary Structure
MDNLQGLMEQAPELIVTYGMKVLFAIIIFVIGKFLSNVAKNLTSKLLKKRKVDNTVVSFV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ANMAWALVFVFTIVATLGQIGVQTASLVAVIGAAGLAVGLALQGSLSNFAAGVLMVLFRP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHC
CRVGDYVEAAGVAGTVDEITIFSTRLLTPDNKVIIAPNSAMMNGTIVNYSAMDTRRLDLV
CCCCCHHHHCCCCCCHHHHEEEHEEEECCCCEEEECCCCCEECCEEEEEECCCCEEEEEE
IGVSYDANLAETKKVLTDILENSQYVLKDPAYTVAVAELADSSVNFVVRPWVKGSDYWPA
EEECCCCCHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHCCCCCCEEEECCCCCCCCCCH
HFEILEQIKVALDEAGIGIPYPQMDLYVKETPAA
HHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12542086 [H]