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The map label for this gene is btuB [H]

Identifier: 157960966

GI number: 157960966

Start: 1373454

End: 1375412

Strand: Direct

Name: btuB [H]

Synonym: Spea_1138

Alternate gene names: 157960966

Gene position: 1373454-1375412 (Clockwise)

Preceding gene: 157960965

Following gene: 157960967

Centisome position: 26.54

GC content: 46.04

Gene sequence:

>1959_bases
ATGAGCTTAAACATTTGCTTATCACTATATGGACATATAAAGATGCATCACAAACTAAAATTAATAGCCCTCGCTTTATC
TGTTACTAGTTTTACCTCTTTTGCGGATGAAATTGAAAAGATCACTGTTCACGGTTTACAACATTATGAGGATGGTACCT
ACACCAACGTCTATCTAGGCAGTGATGTTACGGTTATTAGTAATGAGATGATCCATGCTGCCGGCGGGGTGGATATTAAT
AAAATATTAAGTCAGTTTGTTCCGGGGTTATTCGCCAATGGACGAGCTGGGCGCCATACCGATACCGATTACTCACTGCA
AGGGAGTCGGCCACAAGATATTCTCTGGCTGCTAGACGGTAATCGGCTAAACAACCGCCTTTATGGCTCGACTTATACCG
ATAGCATCAATCCCATGCTGATTGAGCGCATTGAAGTAATCAAAGGTCCTCAGAGTGTACTCTTTGGTTCGGAAGCGATT
GCGGGTGTCATTAATATCATTACTAAAAGTTATCAGGGAAGTGATGCTGGAGAGCTGAGTTTCGCTGCTGACACCTTGCC
TAGCTATGATCTCTCCGGTTTTGTTAGCACTGAAGTTGACTCGGTGCAATTGAGCTTAGCCGCAAGCTCAAGTCAGTCTG
AAGGCTATGCACTCTGGCCCGATGAAGAGTATTCACCAACGGCGGTATTAGATAAAGAGCGCGGCTATCAGATGAATAAC
CTAAATGCCAAGCTTCGTTGGTCGCTCAGTGATGCTCAGCAACTTACTGTTTTTGCCCAGTATAGCGATGGCTTGCTCGA
ACGCCCGTTAGCCTATGATGCGATATTAAGTGAAAATGAGCGCAAGCAAACTATCGCCTATGCAGATTGGCAGCTTGAGG
TTTCTGAGCAGTTACTGTTGCAAGCTAAGGTTAATTACCAGCATTGGGATTCGCAATACAGTGAGATCACTTTGGACGAG
AAGGGTAATGCGACAGTGACATATTGGCAGAAATATTGGGGCTTTAAAGATCTCGGCGCAACGGTTAGTGCTAATTATCA
AACACAGGCGGGAAGTCAGTGGGTTTTCGGTGGCCAATATGAAGAGTATGAAGGCGCCGATGAGGTGATGTTGTTCACCG
CAGAAAAAGATCATTCCTACTCACTATTTAGCCAGTATCAACCTAAGTTTGAAGCCTTGCCGGATACACAATTATCGATT
GGTGCCAGAGTCAATCGGTTAGCAAATGAAGAAACCATCTGGGTGGCTAACCTCGCATTAACTCAGGAACTCACCCCGCG
ACTGCAACTTAAGGGCAGTCTAGGTAATGGTTACCGACAACCGACAGCTTCAGAGCTGCACTCCCAAGTCGGCACACTCG
GAAATGCCGAGCTAGTTGCCGAAACGAGCCAAAGCATTAATTTGGGGCTTCATTATCAGGGAGATGTATCTCTCACAGTC
GATGGTTTTTGGCGCAGTATCGATAACCTTATCGAGCCTCGCGCTATGACTGAAGTTGAATTTATTTATGACAACCTCGA
CGCCCGTGTTGAGAGCTATGGTATCGATCTACACCTTGTTAGTGAGCTGACACCACAGCTACTCATGGAGCTTAGTGGCA
GCTATGCCTCTGCCGCAAGGCAAGATACTGGAGAGCAGATTGAGCGTGTGCCAGAATATACTGGATTTGCCAAACTTAAC
TATGATGTGAATGACGATATATCTGTCTGGATGAGTGGCCAGTACGTGGGGGAGTTTGTCGACTACGGACTGATAGCAGG
GGATTACCTATTAACCAATATTGGCATTCAAGCTTGGATAACGGCAGAGCAGGAGCAGCAACTCTCTCTGACTGTCGATA
ACCTGTTTGATAGTGAGGTGATCCAAAGTATTTTTAAGCACGGTCACAAAGCGCAGTACCCTATTGCCAGCTTAGGTGCG
CCGCGCACATTGCAGCTGCAATATCGTTACCTCTTCTAG

Upstream 100 bases:

>100_bases
GATGGTAATACTTTTTTAAAATTCGTCATACTTTTGGCTTGTGAGTTGATCTTAATCAGATTTCTTTAAATGATGGATAT
ATATAGTCCGTGTTGAAAAA

Downstream 100 bases:

>100_bases
TTTTCCTTCGCTCAGCTAAACAAACTTGATATAGGGGCTTAAGCCCCTTGTAAGCGATATCAGCATGGGAGGTAAAATCG
AAAACTGACAGATATTAACT

Product: TonB-dependent receptor plug

Products: NA

Alternate protein names: Cobalamin receptor; Outer membrane cobalamin translocator [H]

Number of amino acids: Translated: 652; Mature: 651

Protein sequence:

>652_residues
MSLNICLSLYGHIKMHHKLKLIALALSVTSFTSFADEIEKITVHGLQHYEDGTYTNVYLGSDVTVISNEMIHAAGGVDIN
KILSQFVPGLFANGRAGRHTDTDYSLQGSRPQDILWLLDGNRLNNRLYGSTYTDSINPMLIERIEVIKGPQSVLFGSEAI
AGVINIITKSYQGSDAGELSFAADTLPSYDLSGFVSTEVDSVQLSLAASSSQSEGYALWPDEEYSPTAVLDKERGYQMNN
LNAKLRWSLSDAQQLTVFAQYSDGLLERPLAYDAILSENERKQTIAYADWQLEVSEQLLLQAKVNYQHWDSQYSEITLDE
KGNATVTYWQKYWGFKDLGATVSANYQTQAGSQWVFGGQYEEYEGADEVMLFTAEKDHSYSLFSQYQPKFEALPDTQLSI
GARVNRLANEETIWVANLALTQELTPRLQLKGSLGNGYRQPTASELHSQVGTLGNAELVAETSQSINLGLHYQGDVSLTV
DGFWRSIDNLIEPRAMTEVEFIYDNLDARVESYGIDLHLVSELTPQLLMELSGSYASAARQDTGEQIERVPEYTGFAKLN
YDVNDDISVWMSGQYVGEFVDYGLIAGDYLLTNIGIQAWITAEQEQQLSLTVDNLFDSEVIQSIFKHGHKAQYPIASLGA
PRTLQLQYRYLF

Sequences:

>Translated_652_residues
MSLNICLSLYGHIKMHHKLKLIALALSVTSFTSFADEIEKITVHGLQHYEDGTYTNVYLGSDVTVISNEMIHAAGGVDIN
KILSQFVPGLFANGRAGRHTDTDYSLQGSRPQDILWLLDGNRLNNRLYGSTYTDSINPMLIERIEVIKGPQSVLFGSEAI
AGVINIITKSYQGSDAGELSFAADTLPSYDLSGFVSTEVDSVQLSLAASSSQSEGYALWPDEEYSPTAVLDKERGYQMNN
LNAKLRWSLSDAQQLTVFAQYSDGLLERPLAYDAILSENERKQTIAYADWQLEVSEQLLLQAKVNYQHWDSQYSEITLDE
KGNATVTYWQKYWGFKDLGATVSANYQTQAGSQWVFGGQYEEYEGADEVMLFTAEKDHSYSLFSQYQPKFEALPDTQLSI
GARVNRLANEETIWVANLALTQELTPRLQLKGSLGNGYRQPTASELHSQVGTLGNAELVAETSQSINLGLHYQGDVSLTV
DGFWRSIDNLIEPRAMTEVEFIYDNLDARVESYGIDLHLVSELTPQLLMELSGSYASAARQDTGEQIERVPEYTGFAKLN
YDVNDDISVWMSGQYVGEFVDYGLIAGDYLLTNIGIQAWITAEQEQQLSLTVDNLFDSEVIQSIFKHGHKAQYPIASLGA
PRTLQLQYRYLF
>Mature_651_residues
SLNICLSLYGHIKMHHKLKLIALALSVTSFTSFADEIEKITVHGLQHYEDGTYTNVYLGSDVTVISNEMIHAAGGVDINK
ILSQFVPGLFANGRAGRHTDTDYSLQGSRPQDILWLLDGNRLNNRLYGSTYTDSINPMLIERIEVIKGPQSVLFGSEAIA
GVINIITKSYQGSDAGELSFAADTLPSYDLSGFVSTEVDSVQLSLAASSSQSEGYALWPDEEYSPTAVLDKERGYQMNNL
NAKLRWSLSDAQQLTVFAQYSDGLLERPLAYDAILSENERKQTIAYADWQLEVSEQLLLQAKVNYQHWDSQYSEITLDEK
GNATVTYWQKYWGFKDLGATVSANYQTQAGSQWVFGGQYEEYEGADEVMLFTAEKDHSYSLFSQYQPKFEALPDTQLSIG
ARVNRLANEETIWVANLALTQELTPRLQLKGSLGNGYRQPTASELHSQVGTLGNAELVAETSQSINLGLHYQGDVSLTVD
GFWRSIDNLIEPRAMTEVEFIYDNLDARVESYGIDLHLVSELTPQLLMELSGSYASAARQDTGEQIERVPEYTGFAKLNY
DVNDDISVWMSGQYVGEFVDYGLIAGDYLLTNIGIQAWITAEQEQQLSLTVDNLFDSEVIQSIFKHGHKAQYPIASLGAP
RTLQLQYRYLF

Specific function: Involved in the active translocation of vitamin B12 (cyanocobalamin) across the outer membrane to the periplasmic space. It derives its energy for transport by interacting with the trans-periplasmic membrane protein TonB [H]

COG id: COG4206

COG function: function code H; Outer membrane cobalamin receptor protein

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TonB-dependent receptor family. BtuB (TC 1.B.14.3.1) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790405, Length=207, Percent_Identity=27.536231884058, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012910
- InterPro:   IPR000531
- InterPro:   IPR010916 [H]

Pfam domain/function: PF07715 Plug; PF00593 TonB_dep_Rec [H]

EC number: NA

Molecular weight: Translated: 72818; Mature: 72687

Theoretical pI: Translated: 4.29; Mature: 4.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLNICLSLYGHIKMHHKLKLIALALSVTSFTSFADEIEKITVHGLQHYEDGTYTNVYLG
CCEEEEEEEEHHEEHHHHEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEC
SDVTVISNEMIHAAGGVDINKILSQFVPGLFANGRAGRHTDTDYSLQGSRPQDILWLLDG
CCEEEEECCEEEECCCCCHHHHHHHHCCCEECCCCCCCCCCCCCEECCCCCCEEEEEEEC
NRLNNRLYGSTYTDSINPMLIERIEVIKGPQSVLFGSEAIAGVINIITKSYQGSDAGELS
CCCCCEECCCCCCCCCCHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHHCCCCCCCCCEE
FAADTLPSYDLSGFVSTEVDSVQLSLAASSSQSEGYALWPDEEYSPTAVLDKERGYQMNN
EHHCCCCCCCCCCEEECCCCEEEEEEEECCCCCCCEEECCCCCCCCCEEEECCCCCEECC
LNAKLRWSLSDAQQLTVFAQYSDGLLERPLAYDAILSENERKQTIAYADWQLEVSEQLLL
CCEEEEEECCCCCEEEEEEECCCCHHHCCCHHHHHHCCCCCCCEEEEEEEEEEHHHHHHH
QAKVNYQHWDSQYSEITLDEKGNATVTYWQKYWGFKDLGATVSANYQTQAGSQWVFGGQY
HHCCCHHHCCCCCCEEEECCCCCEEEEEHHHHCCHHHCCCEEECCCCCCCCCEEEECCCC
EEYEGADEVMLFTAEKDHSYSLFSQYQPKFEALPDTQLSIGARVNRLANEETIWVANLAL
CCCCCCCEEEEEEECCCCCCHHHHHCCCCHHCCCCCCCHHHHHHHHHCCCCEEEEEEEEE
TQELTPRLQLKGSLGNGYRQPTASELHSQVGTLGNAELVAETSQSINLGLHYQGDVSLTV
HHHCCCCEEEEECCCCCCCCCCHHHHHHHHCCCCCCEEEEECCCCEEEEEEECCCEEEEE
DGFWRSIDNLIEPRAMTEVEFIYDNLDARVESYGIDLHLVSELTPQLLMELSGSYASAAR
HHHHHHHHHHHCCCHHHHHHHHHHCHHHHHHHHCCCEEEHHHCCHHHHHHHCCCHHHHHH
QDTGEQIERVPEYTGFAKLNYDVNDDISVWMSGQYVGEFVDYGLIAGDYLLTNIGIQAWI
HCHHHHHHHCCCCCCEEEEECCCCCCEEEEECCHHHHHHHHHHHHCCCHHHHHCCEEEEE
TAEQEQQLSLTVDNLFDSEVIQSIFKHGHKAQYPIASLGAPRTLQLQYRYLF
ECCCCCEEEEEHHHHCCHHHHHHHHHCCCCCCCCHHHCCCCEEEEEEEEEEC
>Mature Secondary Structure 
SLNICLSLYGHIKMHHKLKLIALALSVTSFTSFADEIEKITVHGLQHYEDGTYTNVYLG
CEEEEEEEEHHEEHHHHEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEC
SDVTVISNEMIHAAGGVDINKILSQFVPGLFANGRAGRHTDTDYSLQGSRPQDILWLLDG
CCEEEEECCEEEECCCCCHHHHHHHHCCCEECCCCCCCCCCCCCEECCCCCCEEEEEEEC
NRLNNRLYGSTYTDSINPMLIERIEVIKGPQSVLFGSEAIAGVINIITKSYQGSDAGELS
CCCCCEECCCCCCCCCCHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHHCCCCCCCCCEE
FAADTLPSYDLSGFVSTEVDSVQLSLAASSSQSEGYALWPDEEYSPTAVLDKERGYQMNN
EHHCCCCCCCCCCEEECCCCEEEEEEEECCCCCCCEEECCCCCCCCCEEEECCCCCEECC
LNAKLRWSLSDAQQLTVFAQYSDGLLERPLAYDAILSENERKQTIAYADWQLEVSEQLLL
CCEEEEEECCCCCEEEEEEECCCCHHHCCCHHHHHHCCCCCCCEEEEEEEEEEHHHHHHH
QAKVNYQHWDSQYSEITLDEKGNATVTYWQKYWGFKDLGATVSANYQTQAGSQWVFGGQY
HHCCCHHHCCCCCCEEEECCCCCEEEEEHHHHCCHHHCCCEEECCCCCCCCCEEEECCCC
EEYEGADEVMLFTAEKDHSYSLFSQYQPKFEALPDTQLSIGARVNRLANEETIWVANLAL
CCCCCCCEEEEEEECCCCCCHHHHHCCCCHHCCCCCCCHHHHHHHHHCCCCEEEEEEEEE
TQELTPRLQLKGSLGNGYRQPTASELHSQVGTLGNAELVAETSQSINLGLHYQGDVSLTV
HHHCCCCEEEEECCCCCCCCCCHHHHHHHHCCCCCCEEEEECCCCEEEEEEECCCEEEEE
DGFWRSIDNLIEPRAMTEVEFIYDNLDARVESYGIDLHLVSELTPQLLMELSGSYASAAR
HHHHHHHHHHHCCCHHHHHHHHHHCHHHHHHHHCCCEEEHHHCCHHHHHHHCCCHHHHHH
QDTGEQIERVPEYTGFAKLNYDVNDDISVWMSGQYVGEFVDYGLIAGDYLLTNIGIQAWI
HCHHHHHHHCCCCCCEEEEECCCCCCEEEEECCHHHHHHHHHHHHCCCHHHHHCCEEEEE
TAEQEQQLSLTVDNLFDSEVIQSIFKHGHKAQYPIASLGAPRTLQLQYRYLF
ECCCCCEEEEEHHHHCCHHHHHHHHHCCCCCCCCHHHCCCCEEEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA