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The map label for this gene is trmB [H]

Identifier: 157960937

GI number: 157960937

Start: 1344730

End: 1345443

Strand: Direct

Name: trmB [H]

Synonym: Spea_1109

Alternate gene names: 157960937

Gene position: 1344730-1345443 (Clockwise)

Preceding gene: 157960934

Following gene: 157960938

Centisome position: 25.99

GC content: 46.78

Gene sequence:

>714_bases
ATGAGCGACGTAACAACCGCTGAATTCAATGAAGAGGGTAAATACCTTCGTAAAGTCAGAAGCTTTGTGTTGAGAGAGGG
CCGTCTAACTAAAGGTCAAGCTCAAGCAATGGAGCAGCAATGGCCTAAAATTGGTCTAGATTACACTCCTGAGCCAATCG
ACTTAGTTGAAGTCTTTGGCCGTGAAGCTGATACCGTTTTAGAAATTGGTTTTGGTATGGGCGCATCATTAGTTGCAATG
GCGAAGGCTGCACCAGAATTAAACTTTATTGGTATTGAAGTGCATAAACCTGGTGTTGGTGCCTGTCTGGCAGAAGCTGC
AGAAGCTGGCGTGACCAACCTACGTGTTTATCACCATGATGCGATTGAAGTGCTCGAGAACAGCATTGCCGAAGGCAGCC
TGGCTTGTGTGCAATTATTCTTCCCAGATCCTTGGCATAAGACTCGTCACCATAAGCGTCGTATCGTTCAAGCGCCATTT
GCTGAATTGATCCGTAGCAAACTTAAGGTTGGCGGTGTATTCCATCTAGCAACAGATTGGGAAAACTACAGCGAGCATAT
GCTGGAAGTGATGACTGCAGCGCCTGGTTATAAAAACCAATCTGCAACAGGTGATGTCGTAGAGCGTCCAGCTCACCGTC
CGCTAACTAAATTCGAAGCGCGTGGCCACCGTTTAGGTCATGGTGTTTGGGATCTTATGTTTGAGCGCGTTTAA

Upstream 100 bases:

>100_bases
GCCACAATAAGGCTTGCACTGAAGGTTTAACTTTGGATAATGCCTTTCTTTTTTAAATAATAGCTGTCTAATGCTAGCGC
AGCCTTAAGCGAGGGCGATA

Downstream 100 bases:

>100_bases
GCGTTTAATAAGAAAGTAAACTTTAAAATAAGCTATTTAGGAGAGTAACCATGGCAGCAAACCGTAGCCGTCGCTTACGT
AAGAAATTACGCGTTGATGA

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase [H]

Number of amino acids: Translated: 237; Mature: 236

Protein sequence:

>237_residues
MSDVTTAEFNEEGKYLRKVRSFVLREGRLTKGQAQAMEQQWPKIGLDYTPEPIDLVEVFGREADTVLEIGFGMGASLVAM
AKAAPELNFIGIEVHKPGVGACLAEAAEAGVTNLRVYHHDAIEVLENSIAEGSLACVQLFFPDPWHKTRHHKRRIVQAPF
AELIRSKLKVGGVFHLATDWENYSEHMLEVMTAAPGYKNQSATGDVVERPAHRPLTKFEARGHRLGHGVWDLMFERV

Sequences:

>Translated_237_residues
MSDVTTAEFNEEGKYLRKVRSFVLREGRLTKGQAQAMEQQWPKIGLDYTPEPIDLVEVFGREADTVLEIGFGMGASLVAM
AKAAPELNFIGIEVHKPGVGACLAEAAEAGVTNLRVYHHDAIEVLENSIAEGSLACVQLFFPDPWHKTRHHKRRIVQAPF
AELIRSKLKVGGVFHLATDWENYSEHMLEVMTAAPGYKNQSATGDVVERPAHRPLTKFEARGHRLGHGVWDLMFERV
>Mature_236_residues
SDVTTAEFNEEGKYLRKVRSFVLREGRLTKGQAQAMEQQWPKIGLDYTPEPIDLVEVFGREADTVLEIGFGMGASLVAMA
KAAPELNFIGIEVHKPGVGACLAEAAEAGVTNLRVYHHDAIEVLENSIAEGSLACVQLFFPDPWHKTRHHKRRIVQAPFA
ELIRSKLKVGGVFHLATDWENYSEHMLEVMTAAPGYKNQSATGDVVERPAHRPLTKFEARGHRLGHGVWDLMFERV

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA [H]

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family [H]

Homologues:

Organism=Escherichia coli, GI1789330, Length=236, Percent_Identity=66.5254237288136, Blast_Score=337, Evalue=3e-94,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003358 [H]

Pfam domain/function: PF02390 Methyltransf_4 [H]

EC number: =2.1.1.33 [H]

Molecular weight: Translated: 26458; Mature: 26326

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDVTTAEFNEEGKYLRKVRSFVLREGRLTKGQAQAMEQQWPKIGLDYTPEPIDLVEVFG
CCCCCCCHHCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHC
READTVLEIGFGMGASLVAMAKAAPELNFIGIEVHKPGVGACLAEAAEAGVTNLRVYHHD
CCCHHHHHHCCCCCHHHHHHHHCCCCCEEEEEEEECCCHHHHHHHHHHCCCCEEEEEHHH
AIEVLENSIAEGSLACVQLFFPDPWHKTRHHKRRIVQAPFAELIRSKLKVGGVFHLATDW
HHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHCHHHHHHHHHHHHCCEEEEECCH
ENYSEHMLEVMTAAPGYKNQSATGDVVERPAHRPLTKFEARGHRLGHGVWDLMFERV
HHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCCHHHHHHCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
SDVTTAEFNEEGKYLRKVRSFVLREGRLTKGQAQAMEQQWPKIGLDYTPEPIDLVEVFG
CCCCCCHHCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHC
READTVLEIGFGMGASLVAMAKAAPELNFIGIEVHKPGVGACLAEAAEAGVTNLRVYHHD
CCCHHHHHHCCCCCHHHHHHHHCCCCCEEEEEEEECCCHHHHHHHHHHCCCCEEEEEHHH
AIEVLENSIAEGSLACVQLFFPDPWHKTRHHKRRIVQAPFAELIRSKLKVGGVFHLATDW
HHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHCHHHHHHHHHHHHCCEEEEECCH
ENYSEHMLEVMTAAPGYKNQSATGDVVERPAHRPLTKFEARGHRLGHGVWDLMFERV
HHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCCHHHHHHCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA