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The map label for this gene is narQ [H]

Identifier: 157960658

GI number: 157960658

Start: 1028876

End: 1030588

Strand: Reverse

Name: narQ [H]

Synonym: Spea_0830

Alternate gene names: 157960658

Gene position: 1030588-1028876 (Counterclockwise)

Preceding gene: 157960660

Following gene: 157960657

Centisome position: 19.92

GC content: 44.13

Gene sequence:

>1713_bases
ATGAAAAAAGGCAGACTCACTTCGGCAATTTGGGGCTTCATGCTCACACTCATTCTATTGTCGAGTGGCCTAGCTATTTT
CGCCATCATCAATTTGTCTTATAGCCTAGGTGATGCCAGGGCCATTAACGCCTCAGGTTCGCTACGTATGCAAAGTTACC
GACTGATGTTTTATGCAAACTCAGGCAGTGAAGGCGCCAACGACAAAATTAAAGAGTTCGAAAATACACTGCACTCTCAA
GCACTTAAGCAATCAATGGGTTGGTATACTCCTCTGGATTTAAAAGAGAAGTATTTGAGCGTTATCGATAAATGGCAGGT
CATGCGCTACTACATAGAATCTGAAAATTCCCGATTATATGCTGCGGCGCTAAAAGATTTTGTCGATACAATCGATCTTT
TCGTGCTCGAAACAGAACAATATGCAGCCCTAAAACTGAGGTTTTTAGCCGTAAGTCAAATAACAGGATTAGCATTAATG
CTGTTAATTGCTTTCTTTACTGTAAGACTCGCAAAACGCAAGGTTGTTAAACCGCTGCAACAATTGATGCAGGCTGCAGA
TACAATTTCACAAGGTAACTTTAATGTAGAGATGCCTAAAACAGAGTACCTAGAATTAAGTGCATTAAGCAGTGCTTTGG
ACTCTACAGTGAAAAAGCTGGCTGGTGTTTACCAAGGGCTTGAGGAACAGGTACAGGAAAAAACCATCGCCTTGACCCGT
GCTAACAACGAATTGACCTTACTCTACGATAGCCTAGTGATGTTACATTCCAGCAAACTCGACTCTAATAGCTTGACCCT
AGCGCTCAACCAGCTTCAAACCCACCAGAGTGAGGCCCATTTACGCCTGGTCATGGTTCAAGATGGTGAGGTTGTCGACA
TCATCAGCACAGACAACGATGGTGATTGGCCGCACAATGGTGAAAACACCCAACAATTCCCATTACAATTTGAGGACAAC
AACTTAGGTTACTTAGAAGTACTCTCCTCTAAGCCCTTCTCTTTCCAGCTGTTTAATAACTTTGCCATCATGCTTGCCCG
CTCTATGGTGATCTATAATGCAGGTGAGCAGCGTCAGCAACTCGCCCTTATGGAAGAGCGTGGCGTGATTGCCAGAGAGC
TACATGACTCCCTTGGGCAGCTACTTTCTTTCCTAAAGATCCAAGTTAATCTACTCTCTAAAGAGTTAGATAAGACCTCA
AAGAGCCCTAGGGCAGCAGAGCAGTTACAAGAAATTAATGAAGGTGTTAATACCGCCTACGTGCAGCTACGAGAGTTATT
ATCTACTTTCAGGCTCACCATCAAAGACCCAAACCTAGGCCATGCGATAGAGGCCATGATAGAGCAGCTACGTGGTCAGT
CAGATGCCATTATTAGCTTGGATTACAAATTACCTTCGCAGCTATTAGGTGCTCATCAACATATTCATATTTTACAGCTC
ACTCGTGAAGCCACCTTAAACGCAATAAAACATGCCAACGCCAAACATATCCATATTAGCTGCCAGAAGGTATCGAGGGA
AAAGGTGGTGTTCAGTATCAGTGATGACGGCATTGGTATCGAGGAGTTAAAAGAAAGGGATCAACATTTTGGTCTAGGAA
TTATGCATGAACGCGCCAGCCGCCTCTCGGGTGTGGTAGAATTTAGCCAGAATAAAAAAGGAGGGGCGACTGTGACCCTT
ACCTTCCCTCCTCAACAGGAGCCAAGCGAATAG

Upstream 100 bases:

>100_bases
GTTGCTCACACTTTTGCGCATTTGTTAATGTAATCAAGTATCCACTAATATTAAGTAGTTATAAATTAGTAATACATAAA
TTCTAATAACGAGATTTTTT

Downstream 100 bases:

>100_bases
ATTTCAACTTCTATGCTGCTGATTTTTGAGGGCGGTTAATGGATTTTTGCGTTATTACTTTGACGAATATACCTCAGCAG
GAGGCCTTAAAATGGGAAAG

Product: nitrate/nitrite sensor protein NarQ

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 570; Mature: 570

Protein sequence:

>570_residues
MKKGRLTSAIWGFMLTLILLSSGLAIFAIINLSYSLGDARAINASGSLRMQSYRLMFYANSGSEGANDKIKEFENTLHSQ
ALKQSMGWYTPLDLKEKYLSVIDKWQVMRYYIESENSRLYAAALKDFVDTIDLFVLETEQYAALKLRFLAVSQITGLALM
LLIAFFTVRLAKRKVVKPLQQLMQAADTISQGNFNVEMPKTEYLELSALSSALDSTVKKLAGVYQGLEEQVQEKTIALTR
ANNELTLLYDSLVMLHSSKLDSNSLTLALNQLQTHQSEAHLRLVMVQDGEVVDIISTDNDGDWPHNGENTQQFPLQFEDN
NLGYLEVLSSKPFSFQLFNNFAIMLARSMVIYNAGEQRQQLALMEERGVIARELHDSLGQLLSFLKIQVNLLSKELDKTS
KSPRAAEQLQEINEGVNTAYVQLRELLSTFRLTIKDPNLGHAIEAMIEQLRGQSDAIISLDYKLPSQLLGAHQHIHILQL
TREATLNAIKHANAKHIHISCQKVSREKVVFSISDDGIGIEELKERDQHFGLGIMHERASRLSGVVEFSQNKKGGATVTL
TFPPQQEPSE

Sequences:

>Translated_570_residues
MKKGRLTSAIWGFMLTLILLSSGLAIFAIINLSYSLGDARAINASGSLRMQSYRLMFYANSGSEGANDKIKEFENTLHSQ
ALKQSMGWYTPLDLKEKYLSVIDKWQVMRYYIESENSRLYAAALKDFVDTIDLFVLETEQYAALKLRFLAVSQITGLALM
LLIAFFTVRLAKRKVVKPLQQLMQAADTISQGNFNVEMPKTEYLELSALSSALDSTVKKLAGVYQGLEEQVQEKTIALTR
ANNELTLLYDSLVMLHSSKLDSNSLTLALNQLQTHQSEAHLRLVMVQDGEVVDIISTDNDGDWPHNGENTQQFPLQFEDN
NLGYLEVLSSKPFSFQLFNNFAIMLARSMVIYNAGEQRQQLALMEERGVIARELHDSLGQLLSFLKIQVNLLSKELDKTS
KSPRAAEQLQEINEGVNTAYVQLRELLSTFRLTIKDPNLGHAIEAMIEQLRGQSDAIISLDYKLPSQLLGAHQHIHILQL
TREATLNAIKHANAKHIHISCQKVSREKVVFSISDDGIGIEELKERDQHFGLGIMHERASRLSGVVEFSQNKKGGATVTL
TFPPQQEPSE
>Mature_570_residues
MKKGRLTSAIWGFMLTLILLSSGLAIFAIINLSYSLGDARAINASGSLRMQSYRLMFYANSGSEGANDKIKEFENTLHSQ
ALKQSMGWYTPLDLKEKYLSVIDKWQVMRYYIESENSRLYAAALKDFVDTIDLFVLETEQYAALKLRFLAVSQITGLALM
LLIAFFTVRLAKRKVVKPLQQLMQAADTISQGNFNVEMPKTEYLELSALSSALDSTVKKLAGVYQGLEEQVQEKTIALTR
ANNELTLLYDSLVMLHSSKLDSNSLTLALNQLQTHQSEAHLRLVMVQDGEVVDIISTDNDGDWPHNGENTQQFPLQFEDN
NLGYLEVLSSKPFSFQLFNNFAIMLARSMVIYNAGEQRQQLALMEERGVIARELHDSLGQLLSFLKIQVNLLSKELDKTS
KSPRAAEQLQEINEGVNTAYVQLRELLSTFRLTIKDPNLGHAIEAMIEQLRGQSDAIISLDYKLPSQLLGAHQHIHILQL
TREATLNAIKHANAKHIHISCQKVSREKVVFSISDDGIGIEELKERDQHFGLGIMHERASRLSGVVEFSQNKKGGATVTL
TFPPQQEPSE

Specific function: Acts as a sensor for nitrate/nitrite and transduces signal of nitrate/nitrite availability to the narL/narP proteins. NarQ probably activates narL and narP by phosphorylation. NarQ probably negatively regulates the narL protein by dephosphorylation [H]

COG id: COG3850

COG function: function code T; Signal transduction histidine kinase, nitrate/nitrite-specific

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1788812, Length=563, Percent_Identity=36.7673179396092, Blast_Score=323, Evalue=2e-89,
Organism=Escherichia coli, GI1787474, Length=577, Percent_Identity=31.0225303292894, Blast_Score=234, Evalue=9e-63,
Organism=Escherichia coli, GI87082311, Length=195, Percent_Identity=24.1025641025641, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR016380
- InterPro:   IPR011712
- InterPro:   IPR005467 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF07730 HisKA_3 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 64028; Mature: 64028

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKGRLTSAIWGFMLTLILLSSGLAIFAIINLSYSLGDARAINASGSLRMQSYRLMFYAN
CCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEECCCCCEEEEEEEEEEEEC
SGSEGANDKIKEFENTLHSQALKQSMGWYTPLDLKEKYLSVIDKWQVMRYYIESENSRLY
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEH
AAALKDFVDTIDLFVLETEQYAALKLRFLAVSQITGLALMLLIAFFTVRLAKRKVVKPLQ
HHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QLMQAADTISQGNFNVEMPKTEYLELSALSSALDSTVKKLAGVYQGLEEQVQEKTIALTR
HHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEE
ANNELTLLYDSLVMLHSSKLDSNSLTLALNQLQTHQSEAHLRLVMVQDGEVVDIISTDND
CCCCEEEHHHHHHHHHCCCCCCCCCEEEHHHHHHCCCCCEEEEEEEECCCEEEEEECCCC
GDWPHNGENTQQFPLQFEDNNLGYLEVLSSKPFSFQLFNNFAIMLARSMVIYNAGEQRQQ
CCCCCCCCCCCCCCEEECCCCCCEEEEECCCCCEEEHHHHHHHHHHHHHHEECCCCHHHH
LALMEERGVIARELHDSLGQLLSFLKIQVNLLSKELDKTSKSPRAAEQLQEINEGVNTAY
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHH
VQLRELLSTFRLTIKDPNLGHAIEAMIEQLRGQSDAIISLDYKLPSQLLGAHQHIHILQL
HHHHHHHHHHEEEECCCCCCHHHHHHHHHHCCCCCCEEEEECCCCHHHHCCCCCEEEEEH
TREATLNAIKHANAKHIHISCQKVSREKVVFSISDDGIGIEELKERDQHFGLGIMHERAS
HHHHHHHHHHCCCCCEEEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHCCCHHHHHHHH
RLSGVVEFSQNKKGGATVTLTFPPQQEPSE
HHHHHHHCCCCCCCCEEEEEEECCCCCCCC
>Mature Secondary Structure
MKKGRLTSAIWGFMLTLILLSSGLAIFAIINLSYSLGDARAINASGSLRMQSYRLMFYAN
CCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEECCCCCEEEEEEEEEEEEC
SGSEGANDKIKEFENTLHSQALKQSMGWYTPLDLKEKYLSVIDKWQVMRYYIESENSRLY
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEH
AAALKDFVDTIDLFVLETEQYAALKLRFLAVSQITGLALMLLIAFFTVRLAKRKVVKPLQ
HHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QLMQAADTISQGNFNVEMPKTEYLELSALSSALDSTVKKLAGVYQGLEEQVQEKTIALTR
HHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEE
ANNELTLLYDSLVMLHSSKLDSNSLTLALNQLQTHQSEAHLRLVMVQDGEVVDIISTDND
CCCCEEEHHHHHHHHHCCCCCCCCCEEEHHHHHHCCCCCEEEEEEEECCCEEEEEECCCC
GDWPHNGENTQQFPLQFEDNNLGYLEVLSSKPFSFQLFNNFAIMLARSMVIYNAGEQRQQ
CCCCCCCCCCCCCCEEECCCCCCEEEEECCCCCEEEHHHHHHHHHHHHHHEECCCCHHHH
LALMEERGVIARELHDSLGQLLSFLKIQVNLLSKELDKTSKSPRAAEQLQEINEGVNTAY
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHH
VQLRELLSTFRLTIKDPNLGHAIEAMIEQLRGQSDAIISLDYKLPSQLLGAHQHIHILQL
HHHHHHHHHHEEEECCCCCCHHHHHHHHHHCCCCCCEEEEECCCCHHHHCCCCCEEEEEH
TREATLNAIKHANAKHIHISCQKVSREKVVFSISDDGIGIEELKERDQHFGLGIMHERAS
HHHHHHHHHHCCCCCEEEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHCCCHHHHHHHH
RLSGVVEFSQNKKGGATVTLTFPPQQEPSE
HHHHHHHCCCCCCCCEEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1528845; 1508040; 9205837; 9278503 [H]