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The map label for this gene is metK [H]

Identifier: 157960615

GI number: 157960615

Start: 972502

End: 973656

Strand: Reverse

Name: metK [H]

Synonym: Spea_0787

Alternate gene names: 157960615

Gene position: 973656-972502 (Counterclockwise)

Preceding gene: 157960624

Following gene: 157960614

Centisome position: 18.82

GC content: 47.71

Gene sequence:

>1155_bases
ATGGCAAAGCACTTGTTTACCTCTGAGTCGGTCTCAGAAGGTCATCCAGATAAAATCGCCGATCAGATTTCTGATGCGGT
ACTAGACGCAATTTTGGAGCAAGATCCAAAAGCTCGTGTAGCATGCGAAACTTATGTCAAAACTGGCATGGTTATGGTTG
GCGGCGAAGTTACCACTTCAGCCTGGGTTGATATCGAAGAGATCACCCGTAAGACTGTACGCGACATTGGCTACACTCAC
TCAGACATGGGTTTTGATGCCGATTCTTGCGCCATCTTAAACGTGATTGGTAAGCAGTCTCCAGACATCAACCAAGGTGT
TGATCGTGCAGACCCTAAAGAACAGGGTGCTGGTGACCAAGGTCTAATGTTCGGTTACGCTAACAACGAAACTGACGTAC
TAATGCCTGCGCCTATCACTTATTCGCACAAGCTTGTTAAGCGTCAGTCTGAAGTTCGTAAAGACAAGACTCTTCCATGG
TTGCGTCCAGACGCGAAGAGCCAGGTGACTTTTGCTTATAACTCAGATGGCAGCATTGCTGGTATCGATGCAGTTGTTCT
ATCTACGCAACACAGCGAAGATGTGACTCAAGCAGACCTAATTGAAGGTGTAATGGAAACCATCATCAAGCCAGTATTAC
CGGCTAAGTGGTTATCTAAAGACACTAAATACTTCATCAACCCAACTGGCCGTTTCGTTATCGGTGGTCCAGTAGGTGAT
TGTGGTCTAACGGGTCGTAAGATCATCGTTGACACATACGGTGGTATGGCTCGTCACGGTGGTGGTGCTTTCTCTGGTAA
AGACCCATCAAAAGTTGACCGTAGTGCAGCATACGCAGCACGTTACGTTGCTAAAAACATCGTTGCAGCAGGTCTTGCAG
ACCGTTGTGAGATCCAAGTTTCTTACGCGATTGGTGTTGCAGAGCCAACTTCAATCAGCATCGAAACATTTGGCACAGCT
AAAGTTGGCGAAGAGCTATTGATCGACCTAGTACGTCGTCACTTCGACCTACGCCCATACGGTCTAACAGAGATGTTGAA
CCTAGCTCGCCCAATCTACCAAGCGACTGCAGCATACGGTCACTTTGGTCGTAACGAGTTCCCGTGGGAAGCAACAGACA
AAGTTGACGCACTACGCGCTGACGCAGGTCTTTAA

Upstream 100 bases:

>100_bases
TGGTGAAGGGGGTGTCATCAACTCGGTTTTACACTAGAATAGCCGTCTAGATGTAGATACTTCTACACATTCAAATTGTT
TAAAGACGAGATTTTCCATC

Downstream 100 bases:

>100_bases
TCGTCACCGATTAGTTCGGCGTTGTTTGTTCAACGCCAATAAAAACCGCCGCTTGGCGGTTTTTTTATGTCCGGAACATT
TATGACTATTTGCCAAGGAT

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 384; Mature: 383

Protein sequence:

>384_residues
MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACETYVKTGMVMVGGEVTTSAWVDIEEITRKTVRDIGYTH
SDMGFDADSCAILNVIGKQSPDINQGVDRADPKEQGAGDQGLMFGYANNETDVLMPAPITYSHKLVKRQSEVRKDKTLPW
LRPDAKSQVTFAYNSDGSIAGIDAVVLSTQHSEDVTQADLIEGVMETIIKPVLPAKWLSKDTKYFINPTGRFVIGGPVGD
CGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSISIETFGTA
KVGEELLIDLVRRHFDLRPYGLTEMLNLARPIYQATAAYGHFGRNEFPWEATDKVDALRADAGL

Sequences:

>Translated_384_residues
MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACETYVKTGMVMVGGEVTTSAWVDIEEITRKTVRDIGYTH
SDMGFDADSCAILNVIGKQSPDINQGVDRADPKEQGAGDQGLMFGYANNETDVLMPAPITYSHKLVKRQSEVRKDKTLPW
LRPDAKSQVTFAYNSDGSIAGIDAVVLSTQHSEDVTQADLIEGVMETIIKPVLPAKWLSKDTKYFINPTGRFVIGGPVGD
CGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSISIETFGTA
KVGEELLIDLVRRHFDLRPYGLTEMLNLARPIYQATAAYGHFGRNEFPWEATDKVDALRADAGL
>Mature_383_residues
AKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACETYVKTGMVMVGGEVTTSAWVDIEEITRKTVRDIGYTHS
DMGFDADSCAILNVIGKQSPDINQGVDRADPKEQGAGDQGLMFGYANNETDVLMPAPITYSHKLVKRQSEVRKDKTLPWL
RPDAKSQVTFAYNSDGSIAGIDAVVLSTQHSEDVTQADLIEGVMETIIKPVLPAKWLSKDTKYFINPTGRFVIGGPVGDC
GLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSISIETFGTAK
VGEELLIDLVRRHFDLRPYGLTEMLNLARPIYQATAAYGHFGRNEFPWEATDKVDALRADAGL

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI4557737, Length=376, Percent_Identity=57.9787234042553, Blast_Score=420, Evalue=1e-117,
Organism=Homo sapiens, GI5174529, Length=376, Percent_Identity=57.4468085106383, Blast_Score=417, Evalue=1e-116,
Organism=Escherichia coli, GI1789311, Length=384, Percent_Identity=83.8541666666667, Blast_Score=671, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17538498, Length=372, Percent_Identity=56.1827956989247, Blast_Score=409, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI25145633, Length=377, Percent_Identity=55.1724137931034, Blast_Score=405, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI17538494, Length=372, Percent_Identity=55.6451612903226, Blast_Score=405, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI17551082, Length=373, Percent_Identity=54.9597855227882, Blast_Score=399, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI32565905, Length=372, Percent_Identity=50.5376344086022, Blast_Score=344, Evalue=4e-95,
Organism=Caenorhabditis elegans, GI32565911, Length=325, Percent_Identity=52.6153846153846, Blast_Score=333, Evalue=9e-92,
Organism=Caenorhabditis elegans, GI25145635, Length=330, Percent_Identity=51.5151515151515, Blast_Score=330, Evalue=1e-90,
Organism=Caenorhabditis elegans, GI32565903, Length=298, Percent_Identity=52.6845637583893, Blast_Score=299, Evalue=1e-81,
Organism=Caenorhabditis elegans, GI32565909, Length=87, Percent_Identity=60.9195402298851, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI32565907, Length=46, Percent_Identity=73.9130434782609, Blast_Score=72, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6320710, Length=376, Percent_Identity=55.5851063829787, Blast_Score=410, Evalue=1e-115,
Organism=Saccharomyces cerevisiae, GI6323209, Length=370, Percent_Identity=56.7567567567568, Blast_Score=409, Evalue=1e-115,
Organism=Drosophila melanogaster, GI45552159, Length=374, Percent_Identity=54.8128342245989, Blast_Score=386, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580529, Length=374, Percent_Identity=54.8128342245989, Blast_Score=386, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580537, Length=374, Percent_Identity=54.8128342245989, Blast_Score=386, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580533, Length=374, Percent_Identity=54.8128342245989, Blast_Score=386, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580535, Length=374, Percent_Identity=54.8128342245989, Blast_Score=386, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580531, Length=374, Percent_Identity=54.8128342245989, Blast_Score=386, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580527, Length=373, Percent_Identity=54.1554959785523, Blast_Score=380, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580525, Length=373, Percent_Identity=54.1554959785523, Blast_Score=380, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580541, Length=372, Percent_Identity=51.8817204301075, Blast_Score=358, Evalue=4e-99,
Organism=Drosophila melanogaster, GI24580539, Length=120, Percent_Identity=55, Blast_Score=130, Evalue=2e-30,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 41605; Mature: 41474

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS00376 ADOMET_SYNTHETASE_1 ; PS00377 ADOMET_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACETYVKTGMVMVGGEVTTS
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCEEEECCCCCCH
AWVDIEEITRKTVRDIGYTHSDMGFDADSCAILNVIGKQSPDINQGVDRADPKEQGAGDQ
HCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCCHHCCCCCCCCHHHCCCCC
GLMFGYANNETDVLMPAPITYSHKLVKRQSEVRKDKTLPWLRPDAKSQVTFAYNSDGSIA
CEEEEECCCCCCEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCEE
GIDAVVLSTQHSEDVTQADLIEGVMETIIKPVLPAKWLSKDTKYFINPTGRFVIGGPVGD
CEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCHHHHCCCCEEEECCCCCEEECCCCCC
CGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLADRCEIQV
CCCCCCEEEEECCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
SYAIGVAEPTSISIETFGTAKVGEELLIDLVRRHFDLRPYGLTEMLNLARPIYQATAAYG
EEEEECCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
HFGRNEFPWEATDKVDALRADAGL
CCCCCCCCCCCCCHHHHHHHCCCC
>Mature Secondary Structure 
AKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACETYVKTGMVMVGGEVTTS
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCEEEECCCCCCH
AWVDIEEITRKTVRDIGYTHSDMGFDADSCAILNVIGKQSPDINQGVDRADPKEQGAGDQ
HCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCCHHCCCCCCCCHHHCCCCC
GLMFGYANNETDVLMPAPITYSHKLVKRQSEVRKDKTLPWLRPDAKSQVTFAYNSDGSIA
CEEEEECCCCCCEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCEE
GIDAVVLSTQHSEDVTQADLIEGVMETIIKPVLPAKWLSKDTKYFINPTGRFVIGGPVGD
CEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCHHHHCCCCEEEECCCCCEEECCCCCC
CGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLADRCEIQV
CCCCCCEEEEECCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
SYAIGVAEPTSISIETFGTAKVGEELLIDLVRRHFDLRPYGLTEMLNLARPIYQATAAYG
EEEEECCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
HFGRNEFPWEATDKVDALRADAGL
CCCCCCCCCCCCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA