Click here to switch to the map view.

The map label for this gene is dcuB [H]

Identifier: 157960513

GI number: 157960513

Start: 846196

End: 847512

Strand: Reverse

Name: dcuB [H]

Synonym: Spea_0685

Alternate gene names: 157960513

Gene position: 847512-846196 (Counterclockwise)

Preceding gene: 157960519

Following gene: 157960512

Centisome position: 16.38

GC content: 47.0

Gene sequence:

>1317_bases
ATGTTTTTCATACATATGCTGCTGTTATTAACTGTTATATTTGTGGGGATCCGCCACGGTGGTGTGGCCTTTGGCTTGCT
TGGTGGTCTGGGCGTATCGGTATTAGCCTTCGTATTTGGCATAGCACCTGGCACACCACCTATCAACGTGATGTTGATTA
TTCTGGCTGTGGTTGCAGCATCGGCTACTCTTGAAGCCACTGGCGGTTTGAAACTACTGGTACGTTACGCCGAAAAACTA
CTGCGTAAACACCCGAATCAAATTGTATTTCTAGGCCCACTTTGCACCTATTCATTAACGGTATTGGTCGGCACGGGCCA
TTCTGTTTATCCACTATTACCTGTTATCTATGATGTCGCCTATAAGAAAGGTATTCGTCCTGAGCGTCCTTTAGCCGTCG
CGACTATCGCCTCACAAATGGGGATTACCGCCAGCCCAATCGCTGCTGCAGCGGCGGTAGTGATTGCGACCTCGATGGAA
AACAACTTAGATATCAGCCTAGTGGACGTGCTTCTGGTGACTATTCCATCGACATTAATCGGTGTGTTAGTCGCTGCGGC
TTGGAGCCTTAATCGTGGTAAAGATCTCGATAAAGACCCTGAATTTCAGGCTCGCATGCAAGATGAAGAGTTTAGAAATA
GCCTGCTCGATAGCGAAGTTGAATCGAGCGATTCGAATCAAAACGACAGCACCGCCAAGAAAGGCTTAAGTATCTTCCTA
TTGGGTATATTGTCAGTGATTGCACTCGCTATGTTCAGCGACCAATTACTACCTGAAGGCGTGAAAATGTCGGTAGCGAT
TCAGTTTATGATGCTATCTGTGGGCGCTGTGATTCTACTCGCAACCAAGATTGATCCTAAGAAGATCGTTCACAGTAATG
TATTCATTGCCGGCATGACGGCGGTGATCATCATCTTTGGTATCGCATGGCTTAGCGACACCATTATTGGCCATCACAAG
TCTTATTTAATCGAATCGGTTAGCGATGTCGTCCACGCTCATCCTTGGTCATTTGCCATCGCGATGTTTACCGCCTCGGT
ATTCTTAAAGAGTCAGGCGGCCACTTTAACCATCATGTTGCCCCTCGGCTTCTCGTTAGGGATCCCTGCTCCGGTACTTA
TCGGTGTCTTGCCAGCTTGTTACGCCTACTTCTTCTTCCCGTTCTACCCAAGTGACTTGGCTGCCATCAGCTTCGATAGA
ACCGGCACCACACATATTGGTAAGTACATAGTGAACCACAGCTTTATTATGCCAGGCTTTATCGGTGTGATAACGGCAAC
TGTCATTGGTTACTCCATATCTATGATGATTAACTAG

Upstream 100 bases:

>100_bases
TGTATAACAGCATTTTAAAAGCTATTACCTATGGCTAGCCCTTGAGTCGGATGTCTATCGGCTAAAGGTTATTGCGCTTA
TCTTCTTAAAGGAACCCGCT

Downstream 100 bases:

>100_bases
TTTCAGCATAAGCTAGCTGCTTTAATAGCAAATTGCAGTTAAGCTCTAGGCGCCCATCGTGGCGCCTTTTCATTAGCTTA
TTTACTTGTGAGAGTCCTAT

Product: anaerobic C4-dicarboxylate transporter

Products: C4-dicarboxylates [Periplasm]; C4-dicarboxylates [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 438; Mature: 438

Protein sequence:

>438_residues
MFFIHMLLLLTVIFVGIRHGGVAFGLLGGLGVSVLAFVFGIAPGTPPINVMLIILAVVAASATLEATGGLKLLVRYAEKL
LRKHPNQIVFLGPLCTYSLTVLVGTGHSVYPLLPVIYDVAYKKGIRPERPLAVATIASQMGITASPIAAAAAVVIATSME
NNLDISLVDVLLVTIPSTLIGVLVAAAWSLNRGKDLDKDPEFQARMQDEEFRNSLLDSEVESSDSNQNDSTAKKGLSIFL
LGILSVIALAMFSDQLLPEGVKMSVAIQFMMLSVGAVILLATKIDPKKIVHSNVFIAGMTAVIIIFGIAWLSDTIIGHHK
SYLIESVSDVVHAHPWSFAIAMFTASVFLKSQAATLTIMLPLGFSLGIPAPVLIGVLPACYAYFFFPFYPSDLAAISFDR
TGTTHIGKYIVNHSFIMPGFIGVITATVIGYSISMMIN

Sequences:

>Translated_438_residues
MFFIHMLLLLTVIFVGIRHGGVAFGLLGGLGVSVLAFVFGIAPGTPPINVMLIILAVVAASATLEATGGLKLLVRYAEKL
LRKHPNQIVFLGPLCTYSLTVLVGTGHSVYPLLPVIYDVAYKKGIRPERPLAVATIASQMGITASPIAAAAAVVIATSME
NNLDISLVDVLLVTIPSTLIGVLVAAAWSLNRGKDLDKDPEFQARMQDEEFRNSLLDSEVESSDSNQNDSTAKKGLSIFL
LGILSVIALAMFSDQLLPEGVKMSVAIQFMMLSVGAVILLATKIDPKKIVHSNVFIAGMTAVIIIFGIAWLSDTIIGHHK
SYLIESVSDVVHAHPWSFAIAMFTASVFLKSQAATLTIMLPLGFSLGIPAPVLIGVLPACYAYFFFPFYPSDLAAISFDR
TGTTHIGKYIVNHSFIMPGFIGVITATVIGYSISMMIN
>Mature_438_residues
MFFIHMLLLLTVIFVGIRHGGVAFGLLGGLGVSVLAFVFGIAPGTPPINVMLIILAVVAASATLEATGGLKLLVRYAEKL
LRKHPNQIVFLGPLCTYSLTVLVGTGHSVYPLLPVIYDVAYKKGIRPERPLAVATIASQMGITASPIAAAAAVVIATSME
NNLDISLVDVLLVTIPSTLIGVLVAAAWSLNRGKDLDKDPEFQARMQDEEFRNSLLDSEVESSDSNQNDSTAKKGLSIFL
LGILSVIALAMFSDQLLPEGVKMSVAIQFMMLSVGAVILLATKIDPKKIVHSNVFIAGMTAVIIIFGIAWLSDTIIGHHK
SYLIESVSDVVHAHPWSFAIAMFTASVFLKSQAATLTIMLPLGFSLGIPAPVLIGVLPACYAYFFFPFYPSDLAAISFDR
TGTTHIGKYIVNHSFIMPGFIGVITATVIGYSISMMIN

Specific function: Responsible for the transport of C4-dicarboxylates from the periplasm across the inner membrane [H]

COG id: COG2704

COG function: function code R; Anaerobic C4-dicarboxylate transporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dcuA/dcuB transporter (TC 2.A.13.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790565, Length=414, Percent_Identity=42.512077294686, Blast_Score=335, Evalue=4e-93,
Organism=Escherichia coli, GI1790580, Length=436, Percent_Identity=37.8440366972477, Blast_Score=260, Evalue=1e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004668 [H]

Pfam domain/function: PF03605 DcuA_DcuB [H]

EC number: NA

Molecular weight: Translated: 46809; Mature: 46809

Theoretical pI: Translated: 7.13; Mature: 7.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFFIHMLLLLTVIFVGIRHGGVAFGLLGGLGVSVLAFVFGIAPGTPPINVMLIILAVVAA
CHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
SATLEATGGLKLLVRYAEKLLRKHPNQIVFLGPLCTYSLTVLVGTGHSVYPLLPVIYDVA
CCCEECCCCHHHHHHHHHHHHHHCCCCEEEECCHHHEEEEEEEECCCCHHHHHHHHHHHH
YKKGIRPERPLAVATIASQMGITASPIAAAAAVVIATSMENNLDISLVDVLLVTIPSTLI
HHCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
GVLVAAAWSLNRGKDLDKDPEFQARMQDEEFRNSLLDSEVESSDSNQNDSTAKKGLSIFL
HHHHHHHHCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
LGILSVIALAMFSDQLLPEGVKMSVAIQFMMLSVGAVILLATKIDPKKIVHSNVFIAGMT
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHEEEECCCHHHHHHCCHHHHHHH
AVIIIFGIAWLSDTIIGHHKSYLIESVSDVVHAHPWSFAIAMFTASVFLKSQAATLTIML
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEEE
PLGFSLGIPAPVLIGVLPACYAYFFFPFYPSDLAAISFDRTGTTHIGKYIVNHSFIMPGF
ECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHEEEECCCCCHHHHHHHHCCCCHHHHH
IGVITATVIGYSISMMIN
HHHHHHHHHHHHEEEEEC
>Mature Secondary Structure
MFFIHMLLLLTVIFVGIRHGGVAFGLLGGLGVSVLAFVFGIAPGTPPINVMLIILAVVAA
CHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
SATLEATGGLKLLVRYAEKLLRKHPNQIVFLGPLCTYSLTVLVGTGHSVYPLLPVIYDVA
CCCEECCCCHHHHHHHHHHHHHHCCCCEEEECCHHHEEEEEEEECCCCHHHHHHHHHHHH
YKKGIRPERPLAVATIASQMGITASPIAAAAAVVIATSMENNLDISLVDVLLVTIPSTLI
HHCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
GVLVAAAWSLNRGKDLDKDPEFQARMQDEEFRNSLLDSEVESSDSNQNDSTAKKGLSIFL
HHHHHHHHCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
LGILSVIALAMFSDQLLPEGVKMSVAIQFMMLSVGAVILLATKIDPKKIVHSNVFIAGMT
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHEEEECCCHHHHHHCCHHHHHHH
AVIIIFGIAWLSDTIIGHHKSYLIESVSDVVHAHPWSFAIAMFTASVFLKSQAATLTIML
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEEE
PLGFSLGIPAPVLIGVLPACYAYFFFPFYPSDLAAISFDRTGTTHIGKYIVNHSFIMPGF
ECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHEEEECCCCCHHHHHHHHCCCCHHHHH
IGVITATVIGYSISMMIN
HHHHHHHHHHHHEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: C4-dicarboxylates [Periplasm]; C4-dicarboxylates [Periplasm] [C]

Specific reaction: C4-dicarboxylates [Periplasm] + C4-dicarboxylates [Periplasm] = C4-dicarboxylates [Periplasm] + C4-dicarboxylates [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]