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The map label for this gene is insF1 [C]

Identifier: 157960509

GI number: 157960509

Start: 843229

End: 843957

Strand: Reverse

Name: insF1 [C]

Synonym: Spea_0681

Alternate gene names: 157960509

Gene position: 843957-843229 (Counterclockwise)

Preceding gene: 157960510

Following gene: 157960505

Centisome position: 16.31

GC content: 43.62

Gene sequence:

>729_bases
ATGGTCATGTATTGGCGCCAGTTTATGCCTAGAGTGGGGACGCGCAAGCTCTACCAGCTCATCAAGCCACAACTGATAGA
ACAAGGGATAAAGCTCGGTAGAGATGGGTTATTTCAGTATTTGAAGCAACAAAATATGCTGGTAAAACCCAGAAAAAATT
ACACTAAAACGACCAATAGCCATCACTGGCTAAGAAAGTATCCCAACTTACTAAAAGACAGAGCGGTCAAGCGTGTTGAA
GAGGTGCTCGTTAGCGATATCACCTACGTGAAATCAGATGAGGGGACGCACTATTTGTCTCTGGTAACAGATGCGTACTC
AAGAAAGATAATGGGATATGAGTTAAGCCATGAAATGAAAGCTTGTGACGTGGTCAAAGCATTGAATATGACGATAAAGA
ATCGTCAGACAACAGGCGATGCCATTCACCACTCAGATAGAGGGATGCAGTACTGCTCAGCTGAGTACCAGGAGACGTTA
GCGGCAAATGGTATTACTCCGTCCATGACAGATGGATATGACTGCTACCAAAATGCACTTGCAGAGCGGGTTAATGGGAT
TTTGAAGCACGAGTTTTTGCTTTATCGCTGCCGTACGATGAAGGAGCTCGATATGTTAATCAAAGAGTCGATAGAGACTT
ATAACCATTTAAGGCCGCATCTTAGTTTAGGGATGAAAACCCCTAATGAAGTACATAAAAAAGCCAGTCGGGAGTTCCAA
CTGGCTTAA

Upstream 100 bases:

>100_bases
AGCCACAGCGAGTCGGCAGTTCAACTTATCTAGACAAGCAATTTATCAATGGAAACAACGCAGAACGGCAAAAGCTGAAA
CGCTTAAACCTGTGATGAAG

Downstream 100 bases:

>100_bases
TAAAAACCGTCAACCTATTTTAGGACTAGACAAACACATCTTACAGGGTATAACGCACTCCGACTGCAATACCGTCGTCT
TCAGTCAGTGCTTGCCTTGC

Product: integrase catalytic subunit

Products: NA

Alternate protein names: ISO-S3 31 kDa protein [H]

Number of amino acids: Translated: 242; Mature: 242

Protein sequence:

>242_residues
MVMYWRQFMPRVGTRKLYQLIKPQLIEQGIKLGRDGLFQYLKQQNMLVKPRKNYTKTTNSHHWLRKYPNLLKDRAVKRVE
EVLVSDITYVKSDEGTHYLSLVTDAYSRKIMGYELSHEMKACDVVKALNMTIKNRQTTGDAIHHSDRGMQYCSAEYQETL
AANGITPSMTDGYDCYQNALAERVNGILKHEFLLYRCRTMKELDMLIKESIETYNHLRPHLSLGMKTPNEVHKKASREFQ
LA

Sequences:

>Translated_242_residues
MVMYWRQFMPRVGTRKLYQLIKPQLIEQGIKLGRDGLFQYLKQQNMLVKPRKNYTKTTNSHHWLRKYPNLLKDRAVKRVE
EVLVSDITYVKSDEGTHYLSLVTDAYSRKIMGYELSHEMKACDVVKALNMTIKNRQTTGDAIHHSDRGMQYCSAEYQETL
AANGITPSMTDGYDCYQNALAERVNGILKHEFLLYRCRTMKELDMLIKESIETYNHLRPHLSLGMKTPNEVHKKASREFQ
LA
>Mature_242_residues
MVMYWRQFMPRVGTRKLYQLIKPQLIEQGIKLGRDGLFQYLKQQNMLVKPRKNYTKTTNSHHWLRKYPNLLKDRAVKRVE
EVLVSDITYVKSDEGTHYLSLVTDAYSRKIMGYELSHEMKACDVVKALNMTIKNRQTTGDAIHHSDRGMQYCSAEYQETL
AANGITPSMTDGYDCYQNALAERVNGILKHEFLLYRCRTMKELDMLIKESIETYNHLRPHLSLGMKTPNEVHKKASREFQ
LA

Specific function: Involved in the transposition of the insertion sequence is3 [C]

COG id: COG2801

COG function: function code L; Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Homo sapiens, GI310129419, Length=192, Percent_Identity=30.7291666666667, Blast_Score=95, Evalue=5e-20,
Organism=Escherichia coli, GI1788405, Length=214, Percent_Identity=30.8411214953271, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1787263, Length=214, Percent_Identity=30.8411214953271, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1786753, Length=214, Percent_Identity=30.8411214953271, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1786570, Length=214, Percent_Identity=30.8411214953271, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1786490, Length=214, Percent_Identity=30.8411214953271, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1789981, Length=165, Percent_Identity=33.9393939393939, Blast_Score=75, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 28327; Mature: 28327

Theoretical pI: Translated: 9.74; Mature: 9.74

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CC
>Mature Secondary Structure
MVMYWRQFMPRVGTRKLYQLIKPQLIEQGIKLGRDGLFQYLKQQNMLVKPRKNYTKTTNS
CCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCEECCCCCCCCCCCC
HHWLRKYPNLLKDRAVKRVEEVLVSDITYVKSDEGTHYLSLVTDAYSRKIMGYELSHEMK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCHHHHHHHH
ACDVVKALNMTIKNRQTTGDAIHHSDRGMQYCSAEYQETLAANGITPSMTDGYDCYQNAL
HHHHHHHHHHHHHCCCCCCHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
AERVNGILKHEFLLYRCRTMKELDMLIKESIETYNHLRPHLSLGMKTPNEVHKKASREFQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCHHHHHHHHHCCCC
LA
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2824781 [H]