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The map label for this gene is pntB [H]

Identifier: 157960478

GI number: 157960478

Start: 804859

End: 806241

Strand: Reverse

Name: pntB [H]

Synonym: Spea_0650

Alternate gene names: 157960478

Gene position: 806241-804859 (Counterclockwise)

Preceding gene: 157960479

Following gene: 157960477

Centisome position: 15.58

GC content: 49.1

Gene sequence:

>1383_bases
ATGAGTACGACGATTATTTACCTCGCCTACTTGGTGGCTGCGAGCTTATTTATTCTAGGTATTAAAGGGCTGACTAAGCC
GCGTACTGCCGTTCGAGGCAACCAGCTTTCCGCGCTAGGTATGTTCATCGCAGTAGTTGTGACCTTGTTTGACCAAAGTA
TTCTCAGCTATGAGTGGATTATTGCAGGTGTGCTGCTTGGTGGCGCCATTGGTGCAGTGATGGCAACTAAGATCCAAGTC
ACCTCTATGCCGCAAATGGTCGCCATGCTAAACGGCTTCGGTGGCGGCGCTTCACTATTTATCGCACTGGCTAACTTTAT
CGACCCGCAATCGGCGACTCATACCGTTGCATTGGTTTCGATTACAGCCACTGTGATTATTGGTGCCGTGACCCTGTCTG
GTTCGTTTATCGCTTTCGCTAAACTGCAAGAGCTGATCTCGGGTAACCCAATTAAGGTGCCGGGTAACAAGTTACTTAAT
GCGCTACTGCTTATCTCAGCAATCGGCCTTGCTATCGCCATCGTGATGGATCCTAGCAATATCAATCTGATCTACGCCTT
AGTGGCAGTATCTCTAGTGCTAGGCTTACTACTAGTAGTACCAATCGGCGGTGCCGATATGCCAGTGGTAATTGCCCTGC
TTAACTCGTATTCGGGTATTGCGGCGGCCACAACTGGTTTCATCACAGGTAACACGGTACTGATTGTGTCGGGTTCACTC
GTTGGTGCATCGGGTATTATCCTGACGCAAATCATGTGTAAAGCGATGAACCGTTCACTGTTTAACGTGCTATTTGGCGT
GATGGCTGAAGGCGGCGAAACAATTGATGCTGATGAAGTTTATGCTGGTAAGGTGACCTCATCGTCACCTGAAGAAGTGG
CCATGCTACTTGAGACTGCCGAGCGTGTAGTGATTGTTCCAGGTTATGGTTTAGCCATGGCGCAGGCGCAGCATGCAGTA
CGTGAACTGGCATCAGTGATGGAAGCTCGTGGCACACAGGTACTCTATGCGATTCACCCTGTTGCCGGTCGTATGCCTGG
CCACATGAACGTGTTATTAGCCGAGGCGGAAGTCCCATACGAGCAGTTGATTGAGATGGATGAGATCAACCCACAGTTTG
AGCAGACTGACGTAGCTATCGTTATTGGTGCCAATGACGTGACCAACCCAATGGCTCGTGAAGACAAAGGTAGCCCAATC
TACGGCATGCCAATTCTTAACGTCGATAAAGCAAAAACCGTGGTTGTGGTTAAGCGTTCATTGAGCCCAGGCTTTGCGGG
TTTACCTAACCCACTGTTCGCTATGGATAACGCATTGATGTTATTTGGCGACGGTAAAAAAGCGATTGTTGAGCTAACCC
AGAGCTTAAAAGAGGCCGATTAA

Upstream 100 bases:

>100_bases
ATTAGGCTTTATTGCCGTGGTACTTGCCACTATCAACGTTGTGGGCGGTTACATGGTAACCAACCGCATGCTAGCCATGT
TTAAAAGGAAATAAGCCACT

Downstream 100 bases:

>100_bases
GGCTAAAGCTTAAATACTAAAAACAGAAAAGGCCGCGAATGCGGCCTTTTGTTTTTCTTCCCCCAAGTAATACCAATCAG
TATAAAGATTTGGTCGCTCA

Product: NAD(P)(+) transhydrogenase

Products: NA

Alternate protein names: Nicotinamide nucleotide transhydrogenase subunit beta; Proton-translocating transhydrogenase NADP(H)-binding component; Pyridine nucleotide transhydrogenase subunit beta; dIII [H]

Number of amino acids: Translated: 460; Mature: 459

Protein sequence:

>460_residues
MSTTIIYLAYLVAASLFILGIKGLTKPRTAVRGNQLSALGMFIAVVVTLFDQSILSYEWIIAGVLLGGAIGAVMATKIQV
TSMPQMVAMLNGFGGGASLFIALANFIDPQSATHTVALVSITATVIIGAVTLSGSFIAFAKLQELISGNPIKVPGNKLLN
ALLLISAIGLAIAIVMDPSNINLIYALVAVSLVLGLLLVVPIGGADMPVVIALLNSYSGIAAATTGFITGNTVLIVSGSL
VGASGIILTQIMCKAMNRSLFNVLFGVMAEGGETIDADEVYAGKVTSSSPEEVAMLLETAERVVIVPGYGLAMAQAQHAV
RELASVMEARGTQVLYAIHPVAGRMPGHMNVLLAEAEVPYEQLIEMDEINPQFEQTDVAIVIGANDVTNPMAREDKGSPI
YGMPILNVDKAKTVVVVKRSLSPGFAGLPNPLFAMDNALMLFGDGKKAIVELTQSLKEAD

Sequences:

>Translated_460_residues
MSTTIIYLAYLVAASLFILGIKGLTKPRTAVRGNQLSALGMFIAVVVTLFDQSILSYEWIIAGVLLGGAIGAVMATKIQV
TSMPQMVAMLNGFGGGASLFIALANFIDPQSATHTVALVSITATVIIGAVTLSGSFIAFAKLQELISGNPIKVPGNKLLN
ALLLISAIGLAIAIVMDPSNINLIYALVAVSLVLGLLLVVPIGGADMPVVIALLNSYSGIAAATTGFITGNTVLIVSGSL
VGASGIILTQIMCKAMNRSLFNVLFGVMAEGGETIDADEVYAGKVTSSSPEEVAMLLETAERVVIVPGYGLAMAQAQHAV
RELASVMEARGTQVLYAIHPVAGRMPGHMNVLLAEAEVPYEQLIEMDEINPQFEQTDVAIVIGANDVTNPMAREDKGSPI
YGMPILNVDKAKTVVVVKRSLSPGFAGLPNPLFAMDNALMLFGDGKKAIVELTQSLKEAD
>Mature_459_residues
STTIIYLAYLVAASLFILGIKGLTKPRTAVRGNQLSALGMFIAVVVTLFDQSILSYEWIIAGVLLGGAIGAVMATKIQVT
SMPQMVAMLNGFGGGASLFIALANFIDPQSATHTVALVSITATVIIGAVTLSGSFIAFAKLQELISGNPIKVPGNKLLNA
LLLISAIGLAIAIVMDPSNINLIYALVAVSLVLGLLLVVPIGGADMPVVIALLNSYSGIAAATTGFITGNTVLIVSGSLV
GASGIILTQIMCKAMNRSLFNVLFGVMAEGGETIDADEVYAGKVTSSSPEEVAMLLETAERVVIVPGYGLAMAQAQHAVR
ELASVMEARGTQVLYAIHPVAGRMPGHMNVLLAEAEVPYEQLIEMDEINPQFEQTDVAIVIGANDVTNPMAREDKGSPIY
GMPILNVDKAKTVVVVKRSLSPGFAGLPNPLFAMDNALMLFGDGKKAIVELTQSLKEAD

Specific function: The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane [H]

COG id: COG1282

COG function: function code C; NAD/NADP transhydrogenase beta subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNT beta subunit family [H]

Homologues:

Organism=Homo sapiens, GI122939155, Length=464, Percent_Identity=41.5948275862069, Blast_Score=319, Evalue=4e-87,
Organism=Homo sapiens, GI122939153, Length=464, Percent_Identity=41.5948275862069, Blast_Score=319, Evalue=4e-87,
Organism=Escherichia coli, GI1787886, Length=465, Percent_Identity=45.8064516129032, Blast_Score=389, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI17550456, Length=463, Percent_Identity=40.6047516198704, Blast_Score=317, Evalue=1e-86,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012136
- InterPro:   IPR004003 [H]

Pfam domain/function: PF02233 PNTB [H]

EC number: =1.6.1.2 [H]

Molecular weight: Translated: 47999; Mature: 47868

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTTIIYLAYLVAASLFILGIKGLTKPRTAVRGNQLSALGMFIAVVVTLFDQSILSYEWI
CCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
IAGVLLGGAIGAVMATKIQVTSMPQMVAMLNGFGGGASLFIALANFIDPQSATHTVALVS
HHHHHHHHHHHHHHHHEEEECCCHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCEEHHHH
ITATVIIGAVTLSGSFIAFAKLQELISGNPIKVPGNKLLNALLLISAIGLAIAIVMDPSN
HHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEECCHHHHHHHHHHHHHHHHEEEEEECCCC
INLIYALVAVSLVLGLLLVVPIGGADMPVVIALLNSYSGIAAATTGFITGNTVLIVSGSL
CHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCHHHHCCEEECCEEEEEECCC
VGASGIILTQIMCKAMNRSLFNVLFGVMAEGGETIDADEVYAGKVTSSSPEEVAMLLETA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCHHHHHHHHHCC
ERVVIVPGYGLAMAQAQHAVRELASVMEARGTQVLYAIHPVAGRMPGHMNVLLAEAEVPY
CCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECHHCCCCCCCEEEEEECCCCH
EQLIEMDEINPQFEQTDVAIVIGANDVTNPMAREDKGSPIYGMPILNVDKAKTVVVVKRS
HHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCEECCCEECCCCCCEEEEEEEC
LSPGFAGLPNPLFAMDNALMLFGDGKKAIVELTQSLKEAD
CCCCCCCCCCCHHHCCCEEEEEECCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
STTIIYLAYLVAASLFILGIKGLTKPRTAVRGNQLSALGMFIAVVVTLFDQSILSYEWI
CCHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
IAGVLLGGAIGAVMATKIQVTSMPQMVAMLNGFGGGASLFIALANFIDPQSATHTVALVS
HHHHHHHHHHHHHHHHEEEECCCHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCEEHHHH
ITATVIIGAVTLSGSFIAFAKLQELISGNPIKVPGNKLLNALLLISAIGLAIAIVMDPSN
HHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEECCHHHHHHHHHHHHHHHHEEEEEECCCC
INLIYALVAVSLVLGLLLVVPIGGADMPVVIALLNSYSGIAAATTGFITGNTVLIVSGSL
CHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCHHHHCCEEECCEEEEEECCC
VGASGIILTQIMCKAMNRSLFNVLFGVMAEGGETIDADEVYAGKVTSSSPEEVAMLLETA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCHHHHHHHHHCC
ERVVIVPGYGLAMAQAQHAVRELASVMEARGTQVLYAIHPVAGRMPGHMNVLLAEAEVPY
CCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECHHCCCCCCCEEEEEECCCCH
EQLIEMDEINPQFEQTDVAIVIGANDVTNPMAREDKGSPIYGMPILNVDKAKTVVVVKRS
HHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCEECCCEECCCCCCEEEEEEEC
LSPGFAGLPNPLFAMDNALMLFGDGKKAIVELTQSLKEAD
CCCCCCCCCCCHHHCCCEEEEEECCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8075801 [H]