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The map label for this gene is ygfZ [C]

Identifier: 157960459

GI number: 157960459

Start: 780304

End: 781275

Strand: Reverse

Name: ygfZ [C]

Synonym: Spea_0630

Alternate gene names: 157960459

Gene position: 781275-780304 (Counterclockwise)

Preceding gene: 157960460

Following gene: 157960456

Centisome position: 15.1

GC content: 47.94

Gene sequence:

>972_bases
ATGACGCTGTCAACTTCTCAGCCATCTTGGCCTCTTAATGGACAACAGCCTGAGCTGCTTTTTTCACAATTATCTCACTT
AGGTTTAATCTCTGTCACTGGCGAGCAGGGTCGCAGCTTTATTCACGGCCAAGTCACCACAGATATCAGCTCACTAGAAA
ATGACCAATGGCGCTGGGGCGCGCACTGCGATCCTAAAGGTAAGATGCTAGCCAGCTTTAGAACCTTCGCTAAAGATGAC
ACCCTTTTCATTATGATGCCAAAAGACACCTTGGCATTAGATTTACCACAACTGCAAAAGTATGCGGTATTTAGCAAAGC
AGAATTAGCCGATATCAGTGATGCTTGGTTACTGCTTGGCGTTGCAGGCGAGCAAGCAAATGCTTGGCTAACGGCGCAGT
TTGGTGAGCTGAGTGCAGAGCTAACATTAATCGATGGCGGCATAATCCTCCATGATGCGGGTCGTTATATTGTGGCTATC
GACAAGACACATGCTGACGCATTTATCGCTAAAATCGAACAACCTATCTTTGATGCAAGTGCATGGCAAACACTGGAGAT
CCTAGCGGGTTACCCAAACCTAGGAGCCAGCCATCAAGGCCAGTTTGTACCGCAGATGTGTAACGTACAGGCTGTGAATG
GTATCAGCTTCAATAAAGGCTGCTACATGGGGCAAGAGACAGTGGCTCGTATGAAGTACCGTGGTGGTAATAAGCGCGCA
CTGTATATTGTTAGCGGCAAAGTATCAGCGCCACTCACAGCCGACAGCCAACTCGAAATAGCGTTGGAAGACGGTGAAGG
ATTCCGCCGCGCAGGTACCATCATCGAGGCGGTTCAACGTGATGGCCAAGTGTTATTAACAGCCGTACTGGCTAACGATA
CTCAGCTTGATGCCAAGCTTAGAGTAGCCGGTGATGAAAGCTCAGAGCTAACACTGATTGCCTTGCCTTATTCTCTAGAA
GATCAAGATTAA

Upstream 100 bases:

>100_bases
TGGTATTGGTGGGCAGCAATAGTTTGAACTGCTGCGCTTGCGCAGTATCAATTTAGCGGTACAATCATTACCCTCTTTGA
TTTAACTCTTGGATCCTCTT

Downstream 100 bases:

>100_bases
ACCTGTTGCTGCTTTGGCTTAAAACAGAAAAGGCGCTTACCGCGCCTTTTTGTTTTCAGCTAGTTTCAGCTCAAATAATT
CAGAGTTATCCAGAGTCACT

Product: glycine cleavage T protein (aminomethyl transferase)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 323; Mature: 322

Protein sequence:

>323_residues
MTLSTSQPSWPLNGQQPELLFSQLSHLGLISVTGEQGRSFIHGQVTTDISSLENDQWRWGAHCDPKGKMLASFRTFAKDD
TLFIMMPKDTLALDLPQLQKYAVFSKAELADISDAWLLLGVAGEQANAWLTAQFGELSAELTLIDGGIILHDAGRYIVAI
DKTHADAFIAKIEQPIFDASAWQTLEILAGYPNLGASHQGQFVPQMCNVQAVNGISFNKGCYMGQETVARMKYRGGNKRA
LYIVSGKVSAPLTADSQLEIALEDGEGFRRAGTIIEAVQRDGQVLLTAVLANDTQLDAKLRVAGDESSELTLIALPYSLE
DQD

Sequences:

>Translated_323_residues
MTLSTSQPSWPLNGQQPELLFSQLSHLGLISVTGEQGRSFIHGQVTTDISSLENDQWRWGAHCDPKGKMLASFRTFAKDD
TLFIMMPKDTLALDLPQLQKYAVFSKAELADISDAWLLLGVAGEQANAWLTAQFGELSAELTLIDGGIILHDAGRYIVAI
DKTHADAFIAKIEQPIFDASAWQTLEILAGYPNLGASHQGQFVPQMCNVQAVNGISFNKGCYMGQETVARMKYRGGNKRA
LYIVSGKVSAPLTADSQLEIALEDGEGFRRAGTIIEAVQRDGQVLLTAVLANDTQLDAKLRVAGDESSELTLIALPYSLE
DQD
>Mature_322_residues
TLSTSQPSWPLNGQQPELLFSQLSHLGLISVTGEQGRSFIHGQVTTDISSLENDQWRWGAHCDPKGKMLASFRTFAKDDT
LFIMMPKDTLALDLPQLQKYAVFSKAELADISDAWLLLGVAGEQANAWLTAQFGELSAELTLIDGGIILHDAGRYIVAID
KTHADAFIAKIEQPIFDASAWQTLEILAGYPNLGASHQGQFVPQMCNVQAVNGISFNKGCYMGQETVARMKYRGGNKRAL
YIVSGKVSAPLTADSQLEIALEDGEGFRRAGTIIEAVQRDGQVLLTAVLANDTQLDAKLRVAGDESSELTLIALPYSLED
QD

Specific function: Folate-binding protein involved in regulating the level of ATP-dnaA and in the modification of some tRNAs. It is probably a key factor in regulatory networks that act via tRNA modification, such as initiation of chromosomal replication [H]

COG id: COG0354

COG function: function code R; Predicted aminomethyltransferase related to GcvT

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA-modifying ygfZ family [H]

Homologues:

Organism=Escherichia coli, GI1789265, Length=330, Percent_Identity=39.0909090909091, Blast_Score=206, Evalue=1e-54,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017703
- InterPro:   IPR006222 [H]

Pfam domain/function: PF01571 GCV_T [H]

EC number: NA

Molecular weight: Translated: 35175; Mature: 35044

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLSTSQPSWPLNGQQPELLFSQLSHLGLISVTGEQGRSFIHGQVTTDISSLENDQWRWG
CCCCCCCCCCCCCCCCHHHHHHHHHHCEEEEEECCCCCEEEEEEEEECHHHCCCCCEEEC
AHCDPKGKMLASFRTFAKDDTLFIMMPKDTLALDLPQLQKYAVFSKAELADISDAWLLLG
CCCCCCCHHHHHHHHHCCCCEEEEEECCCCEEECCHHHHHHHHHCCCHHCCCCCEEEEEE
VAGEQANAWLTAQFGELSAELTLIDGGIILHDAGRYIVAIDKTHADAFIAKIEQPIFDAS
ECCCCCCEEEEEECCCCEEEEEEEECCEEEEECCCEEEEEECCCHHHHHHHHHCCCCCCC
AWQTLEILAGYPNLGASHQGQFVPQMCNVQAVNGISFNKGCYMGQETVARMKYRGGNKRA
CHHEEEEEECCCCCCCCCCCCCCHHHCCEEEECCEEECCCCCCCHHHHHHHHCCCCCCEE
LYIVSGKVSAPLTADSQLEIALEDGEGFRRAGTIIEAVQRDGQVLLTAVLANDTQLDAKL
EEEEECCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCEEEEEEEECCCCEECEEE
RVAGDESSELTLIALPYSLEDQD
EEECCCCCCEEEEEECCCCCCCC
>Mature Secondary Structure 
TLSTSQPSWPLNGQQPELLFSQLSHLGLISVTGEQGRSFIHGQVTTDISSLENDQWRWG
CCCCCCCCCCCCCCCHHHHHHHHHHCEEEEEECCCCCEEEEEEEEECHHHCCCCCEEEC
AHCDPKGKMLASFRTFAKDDTLFIMMPKDTLALDLPQLQKYAVFSKAELADISDAWLLLG
CCCCCCCHHHHHHHHHCCCCEEEEEECCCCEEECCHHHHHHHHHCCCHHCCCCCEEEEEE
VAGEQANAWLTAQFGELSAELTLIDGGIILHDAGRYIVAIDKTHADAFIAKIEQPIFDAS
ECCCCCCEEEEEECCCCEEEEEEEECCEEEEECCCEEEEEECCCHHHHHHHHHCCCCCCC
AWQTLEILAGYPNLGASHQGQFVPQMCNVQAVNGISFNKGCYMGQETVARMKYRGGNKRA
CHHEEEEEECCCCCCCCCCCCCCHHHCCEEEECCEEECCCCCCCHHHHHHHHCCCCCCEE
LYIVSGKVSAPLTADSQLEIALEDGEGFRRAGTIIEAVQRDGQVLLTAVLANDTQLDAKL
EEEEECCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCEEEEEEEECCCCEECEEE
RVAGDESSELTLIALPYSLEDQD
EEECCCCCCEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]