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The map label for this gene is birA [H]

Identifier: 157825805

GI number: 157825805

Start: 668122

End: 668925

Strand: Direct

Name: birA [H]

Synonym: A1C_03700

Alternate gene names: 157825805

Gene position: 668122-668925 (Clockwise)

Preceding gene: 157825804

Following gene: 157825807

Centisome position: 54.27

GC content: 34.33

Gene sequence:

>804_bases
ATGAACATAGGTAAATTTAAACTAATTGTTTTTGATACACTAGACAGTACAAGCTCTGAGGCTATGAGAATAGTTCAGAA
TGCTAAAGTTGATTCAAACTATGCAGTACTAGCCAAATCTCAAACTAACGGACGTGGTAGAAACGGAAAAAACTGGCAGT
CTAGGTCGGGTAACTTACATGTAAGTTTACTAATAAAGCCTGATAAAGAGCTAGCATTATTACCGCAATTATCTTTTGTT
ACAGCACTTGCCATTTATGATTGTATGTCATCCCGTGGCTTGACCACGGGATCCAGTAAACCAACAACTAATGATACTGT
AAGCTGTGTTCTGGAGCCTGTGGTCAAGCCACGGGATGACATTATACAACTAAAATGGCCTAACGATGTTCTCGTGAACG
GTCGGAAAATTGCCGGTATACTTCTTGAATCTGTTAAAATAGAAAATAATTATTACCTTATTATCGGTATCGGTATTAAT
ATTACATATCACCCTGATAATATCGACCAGCCTACTACTAGCTTAATAAGCGAAAATCTACCGCCTATAGAGACGCAAGC
TTTACTTCAAAAATTAATAGAAAATTTTGAAAAATATTATCAAATCTGGCATAATAACGGTTTTTCTTTTATTAGACAAA
AATGGTTAGAACATGCGTACAAGTTACATGAAAATATTAGCGTCAAACATCAAAATGATATAATAACCGGTCTTTTTAAA
GATATCGATAATACTGGTAGAATAATATTGCAACTACCTTCCAAAAAAATAATCTCTTTTTCCACTGCTGAACTTTCTTT
TTAG

Upstream 100 bases:

>100_bases
TCCAGACAAAATAATAAAAATTTCTGTAAATCAGAATTTTTCACTGGATTGCTTTGTCGAATTACTGTGTAATTCTTCTT
GCAATAACGATGACTAAAAA

Downstream 100 bases:

>100_bases
CAAAAAAAATTACTAAATTTAAATTGTAGATTTAATTTTTGTTCACCTATACTTAAGCTCTAATTTAACAAGGTAATAAA
TATATGCCCCAATAGAACAG

Product: biotin--protein ligase

Products: NA

Alternate protein names: Biotin--protein ligase [H]

Number of amino acids: Translated: 267; Mature: 267

Protein sequence:

>267_residues
MNIGKFKLIVFDTLDSTSSEAMRIVQNAKVDSNYAVLAKSQTNGRGRNGKNWQSRSGNLHVSLLIKPDKELALLPQLSFV
TALAIYDCMSSRGLTTGSSKPTTNDTVSCVLEPVVKPRDDIIQLKWPNDVLVNGRKIAGILLESVKIENNYYLIIGIGIN
ITYHPDNIDQPTTSLISENLPPIETQALLQKLIENFEKYYQIWHNNGFSFIRQKWLEHAYKLHENISVKHQNDIITGLFK
DIDNTGRIILQLPSKKIISFSTAELSF

Sequences:

>Translated_267_residues
MNIGKFKLIVFDTLDSTSSEAMRIVQNAKVDSNYAVLAKSQTNGRGRNGKNWQSRSGNLHVSLLIKPDKELALLPQLSFV
TALAIYDCMSSRGLTTGSSKPTTNDTVSCVLEPVVKPRDDIIQLKWPNDVLVNGRKIAGILLESVKIENNYYLIIGIGIN
ITYHPDNIDQPTTSLISENLPPIETQALLQKLIENFEKYYQIWHNNGFSFIRQKWLEHAYKLHENISVKHQNDIITGLFK
DIDNTGRIILQLPSKKIISFSTAELSF
>Mature_267_residues
MNIGKFKLIVFDTLDSTSSEAMRIVQNAKVDSNYAVLAKSQTNGRGRNGKNWQSRSGNLHVSLLIKPDKELALLPQLSFV
TALAIYDCMSSRGLTTGSSKPTTNDTVSCVLEPVVKPRDDIIQLKWPNDVLVNGRKIAGILLESVKIENNYYLIIGIGIN
ITYHPDNIDQPTTSLISENLPPIETQALLQKLIENFEKYYQIWHNNGFSFIRQKWLEHAYKLHENISVKHQNDIITGLFK
DIDNTGRIILQLPSKKIISFSTAELSF

Specific function: Activates biotin to form biotinyl-5'-adenylate and transfers the biotin moiety to biotin-accepting proteins [H]

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Escherichia coli, GI1790408, Length=247, Percent_Identity=22.6720647773279, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004408
- InterPro:   IPR003142
- InterPro:   IPR004143 [H]

Pfam domain/function: PF02237 BPL_C; PF03099 BPL_LipA_LipB [H]

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 30100; Mature: 30100

Theoretical pI: Translated: 8.96; Mature: 8.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIGKFKLIVFDTLDSTSSEAMRIVQNAKVDSNYAVLAKSQTNGRGRNGKNWQSRSGNLH
CCCCEEEEEEEECCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCEE
VSLLIKPDKELALLPQLSFVTALAIYDCMSSRGLTTGSSKPTTNDTVSCVLEPVVKPRDD
EEEEECCCCCEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCCC
IIQLKWPNDVLVNGRKIAGILLESVKIENNYYLIIGIGINITYHPDNIDQPTTSLISENL
EEEEECCCCEEECCHHHEEEEEEEEEECCCEEEEEEEEEEEEECCCCCCCCHHHHHHCCC
PPIETQALLQKLIENFEKYYQIWHNNGFSFIRQKWLEHAYKLHENISVKHQNDIITGLFK
CCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCEEECCHHHHHHHH
DIDNTGRIILQLPSKKIISFSTAELSF
CCCCCCEEEEEECCCCEEEEECCCCCC
>Mature Secondary Structure
MNIGKFKLIVFDTLDSTSSEAMRIVQNAKVDSNYAVLAKSQTNGRGRNGKNWQSRSGNLH
CCCCEEEEEEEECCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCEE
VSLLIKPDKELALLPQLSFVTALAIYDCMSSRGLTTGSSKPTTNDTVSCVLEPVVKPRDD
EEEEECCCCCEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCCC
IIQLKWPNDVLVNGRKIAGILLESVKIENNYYLIIGIGINITYHPDNIDQPTTSLISENL
EEEEECCCCEEECCHHHEEEEEEEEEECCCEEEEEEEEEEEEECCCCCCCCHHHHHHCCC
PPIETQALLQKLIENFEKYYQIWHNNGFSFIRQKWLEHAYKLHENISVKHQNDIITGLFK
CCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCEEECCHHHHHHHH
DIDNTGRIILQLPSKKIISFSTAELSF
CCCCCCEEEEEECCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8422400 [H]