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The map label for this gene is folC [H]

Identifier: 157825802

GI number: 157825802

Start: 664260

End: 665549

Strand: Direct

Name: folC [H]

Synonym: A1C_03665

Alternate gene names: 157825802

Gene position: 664260-665549 (Clockwise)

Preceding gene: 157825801

Following gene: 157825803

Centisome position: 53.96

GC content: 34.88

Gene sequence:

>1290_bases
ATGCTAATTCCGCATTTTCCGGTGCCGACCTGGAAAAATAATATTAAATATGATCTTGAAAATATAGCAAGCCTTTTAAA
AGCCTTAGGTAACCCTCATTTAAGGCTTCCTCCCGTCATACATATAGCAGGTACCAACGGTAAAGGTTCAAGCAGTGCTA
TGCTTAAAAGCATCTTTACGATTGCCGGTTATAAAGTACATTGCTATACATCTCCTCATTTATTCGAATTTAATGAACGA
ATCGTAGTAGCAGGTGAAAAAATCTCGGATAATGAATTATGGCAAGTTTGTGAAGAGGTGAGGGTAGCAAGCGAAAAATT
TAATATCGAACCTAGCTTCTTTGAAGGTACTACGGCAGCAGCATTTGTAGCATTTGAGAAAACAAGAGCCGATATATTAA
TTTTAGAGACGGGACTAGGCGGTCGGTTAGACGCAACAAATATAGTAGAACGACCGTTAATTACTTTAATTACACCTATT
TCATATGATCATATGAATGTGCTTGGGTCAACATTACCACTAATAGCAGTTGAGAAATCCGGTATTATGAAATCTTGCGT
TCCTTGCGTTATAAGTATACAAGCTTTAGCAGTTTATGAAACATTATTTGCAAAAGCTGAAGAGCTAGATGTACCTACTT
TTTGTTATGAATATGATTTCAGTATTAAAAAGGCGGAGAACGGATTTATTTATTCATCACGAAATTTTAGTTATGAATTT
CCTACTCCTTCACTGCTTGGTGATCATCAATTAATAAATGCTGCAAGCGTTATTGCTTTAATAAGTTTGATAAATAAACA
ATTTAATATTAGCAACGACATTATCTCTAAAGGACTGCAAAATACTATTTGGCGGGCTAGAATCGAAAAAATAGAACCGA
CAAAATATTCCAGATTAATAGGTGATAATGTTCAAATTTGGGTAGACACAGCTCATAATAATAGTGGGGCTCAAGTTTTT
GCAAATTGGATTCGTGATAACTTAAAATCACCGATATATTTAATACTCGGTATGACTAAAAATAGGAATATCAAGGAGTT
TTGCTCCTACTTTAAGGATTTGACAATTAAAGGTTACGGTGTTAAAGTTTTATCCGAACCGCTAAGCCATAGTGCAGAAA
CAATAAATTTGGAAGGCAGAAAGAGCGGTATTGATTTTAGCGCAAGCGACTCACTCGAAGATGCGATTAGTGATATAAAG
AAGATAAACGGTGATAATAAAGCAAATATTATAATAACCGGATCGCTTTATCTAGCTTCCGATTTTTATAAATTGCTGTC
TACTCGATGA

Upstream 100 bases:

>100_bases
AACGGGGTAACATAGTATGTTTTACTGGGTTCTCGCTGCCGCAGGAATGATATCAAGTATTGATATATAACTCTATCATT
CATGCAAAAAATCAATAACA

Downstream 100 bases:

>100_bases
ACTGTAAAAATTGGCTACATCGTGCTACAAGAGCGATGGTGCTCACCGATTCTTATACGCTCCACTCCTCGTCTTGTGAA
CTCCTTGCTCTTTTTACAGT

Product: folylpolyglutamate synthase

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolate synthase; Tetrahydrofolylpolyglutamate synthase [H]

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MLIPHFPVPTWKNNIKYDLENIASLLKALGNPHLRLPPVIHIAGTNGKGSSSAMLKSIFTIAGYKVHCYTSPHLFEFNER
IVVAGEKISDNELWQVCEEVRVASEKFNIEPSFFEGTTAAAFVAFEKTRADILILETGLGGRLDATNIVERPLITLITPI
SYDHMNVLGSTLPLIAVEKSGIMKSCVPCVISIQALAVYETLFAKAEELDVPTFCYEYDFSIKKAENGFIYSSRNFSYEF
PTPSLLGDHQLINAASVIALISLINKQFNISNDIISKGLQNTIWRARIEKIEPTKYSRLIGDNVQIWVDTAHNNSGAQVF
ANWIRDNLKSPIYLILGMTKNRNIKEFCSYFKDLTIKGYGVKVLSEPLSHSAETINLEGRKSGIDFSASDSLEDAISDIK
KINGDNKANIIITGSLYLASDFYKLLSTR

Sequences:

>Translated_429_residues
MLIPHFPVPTWKNNIKYDLENIASLLKALGNPHLRLPPVIHIAGTNGKGSSSAMLKSIFTIAGYKVHCYTSPHLFEFNER
IVVAGEKISDNELWQVCEEVRVASEKFNIEPSFFEGTTAAAFVAFEKTRADILILETGLGGRLDATNIVERPLITLITPI
SYDHMNVLGSTLPLIAVEKSGIMKSCVPCVISIQALAVYETLFAKAEELDVPTFCYEYDFSIKKAENGFIYSSRNFSYEF
PTPSLLGDHQLINAASVIALISLINKQFNISNDIISKGLQNTIWRARIEKIEPTKYSRLIGDNVQIWVDTAHNNSGAQVF
ANWIRDNLKSPIYLILGMTKNRNIKEFCSYFKDLTIKGYGVKVLSEPLSHSAETINLEGRKSGIDFSASDSLEDAISDIK
KINGDNKANIIITGSLYLASDFYKLLSTR
>Mature_429_residues
MLIPHFPVPTWKNNIKYDLENIASLLKALGNPHLRLPPVIHIAGTNGKGSSSAMLKSIFTIAGYKVHCYTSPHLFEFNER
IVVAGEKISDNELWQVCEEVRVASEKFNIEPSFFEGTTAAAFVAFEKTRADILILETGLGGRLDATNIVERPLITLITPI
SYDHMNVLGSTLPLIAVEKSGIMKSCVPCVISIQALAVYETLFAKAEELDVPTFCYEYDFSIKKAENGFIYSSRNFSYEF
PTPSLLGDHQLINAASVIALISLINKQFNISNDIISKGLQNTIWRARIEKIEPTKYSRLIGDNVQIWVDTAHNNSGAQVF
ANWIRDNLKSPIYLILGMTKNRNIKEFCSYFKDLTIKGYGVKVLSEPLSHSAETINLEGRKSGIDFSASDSLEDAISDIK
KINGDNKANIIITGSLYLASDFYKLLSTR

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932990, Length=313, Percent_Identity=26.1980830670927, Blast_Score=120, Evalue=4e-27,
Organism=Homo sapiens, GI66932984, Length=312, Percent_Identity=26.2820512820513, Blast_Score=119, Evalue=4e-27,
Organism=Escherichia coli, GI1788654, Length=396, Percent_Identity=26.010101010101, Blast_Score=130, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17553150, Length=371, Percent_Identity=28.3018867924528, Blast_Score=132, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI71984923, Length=371, Percent_Identity=28.3018867924528, Blast_Score=132, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI17553148, Length=371, Percent_Identity=28.3018867924528, Blast_Score=132, Evalue=5e-31,
Organism=Saccharomyces cerevisiae, GI6323760, Length=427, Percent_Identity=24.1217798594848, Blast_Score=130, Evalue=4e-31,
Organism=Saccharomyces cerevisiae, GI6324815, Length=432, Percent_Identity=27.7777777777778, Blast_Score=116, Evalue=9e-27,
Organism=Drosophila melanogaster, GI24641571, Length=316, Percent_Identity=26.8987341772152, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24581568, Length=208, Percent_Identity=33.1730769230769, Blast_Score=99, Evalue=6e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 47759; Mature: 47759

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS01012 FOLYLPOLYGLU_SYNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIPHFPVPTWKNNIKYDLENIASLLKALGNPHLRLPPVIHIAGTNGKGSSSAMLKSIFT
CCCCCCCCCCCCCCCEECHHHHHHHHHHHCCCCEECCCEEEEECCCCCCCHHHHHHHHHH
IAGYKVHCYTSPHLFEFNERIVVAGEKISDNELWQVCEEVRVASEKFNIEPSFFEGTTAA
HCCEEEEEEECCCEEECCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCE
AFVAFEKTRADILILETGLGGRLDATNIVERPLITLITPISYDHMNVLGSTLPLIAVEKS
EEEEEECCCCEEEEEECCCCCCCCHHHHHHCCCEEEECCCCCHHHHHHHCCCCEEEEECC
GIMKSCVPCVISIQALAVYETLFAKAEELDVPTFCYEYDFSIKKAENGFIYSSRNFSYEF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCEEEECCCCEEEECCCCEEEC
PTPSLLGDHQLINAASVIALISLINKQFNISNDIISKGLQNTIWRARIEKIEPTKYSRLI
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
GDNVQIWVDTAHNNSGAQVFANWIRDNLKSPIYLILGMTKNRNIKEFCSYFKDLTIKGYG
CCCEEEEEEECCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHCCEECCCC
VKVLSEPLSHSAETINLEGRKSGIDFSASDSLEDAISDIKKINGDNKANIIITGSLYLAS
HHHHHHHHCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEECHHHHH
DFYKLLSTR
HHHHHHHCC
>Mature Secondary Structure
MLIPHFPVPTWKNNIKYDLENIASLLKALGNPHLRLPPVIHIAGTNGKGSSSAMLKSIFT
CCCCCCCCCCCCCCCEECHHHHHHHHHHHCCCCEECCCEEEEECCCCCCCHHHHHHHHHH
IAGYKVHCYTSPHLFEFNERIVVAGEKISDNELWQVCEEVRVASEKFNIEPSFFEGTTAA
HCCEEEEEEECCCEEECCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCE
AFVAFEKTRADILILETGLGGRLDATNIVERPLITLITPISYDHMNVLGSTLPLIAVEKS
EEEEEECCCCEEEEEECCCCCCCCHHHHHHCCCEEEECCCCCHHHHHHHCCCCEEEEECC
GIMKSCVPCVISIQALAVYETLFAKAEELDVPTFCYEYDFSIKKAENGFIYSSRNFSYEF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCEEEECCCCEEEECCCCEEEC
PTPSLLGDHQLINAASVIALISLINKQFNISNDIISKGLQNTIWRARIEKIEPTKYSRLI
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
GDNVQIWVDTAHNNSGAQVFANWIRDNLKSPIYLILGMTKNRNIKEFCSYFKDLTIKGYG
CCCEEEEEEECCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHCCEECCCC
VKVLSEPLSHSAETINLEGRKSGIDFSASDSLEDAISDIKKINGDNKANIIITGSLYLAS
HHHHHHHHCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEECHHHHH
DFYKLLSTR
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8419299; 9384377; 2553669 [H]