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The map label for this gene is epsE [H]

Identifier: 157825750

GI number: 157825750

Start: 609097

End: 609942

Strand: Direct

Name: epsE [H]

Synonym: A1C_03380

Alternate gene names: 157825750

Gene position: 609097-609942 (Clockwise)

Preceding gene: 157825749

Following gene: 157825751

Centisome position: 49.48

GC content: 30.5

Gene sequence:

>846_bases
ATGCGTATTAACACTCTAAAAATCTCAATAGTTTTACCGGTTTATAATAGGGAGAAGCTATTATCTTTTGCTATTGAGAG
CTGTCTTAATCAAAGCTTTAAAGACTTTGAGCTTATTATTATTGATGATTGCTCAAAAGATAGTAGCGTGATAGTAGCTA
GGAAATATGCAGAGCAGGATTCACGTATTAAGGTAATAGTAAACGAGACCAACAAAACATTACCGGCAAGTTTGAATATT
GCTTTTAAAGAGGCTAAGGGGGAGTATTTTACTTGGACTTCAGATGATAATCTTTTTCATGAAAATGCTTTAGAAAAAAT
GGTTAAGATACTTGATAATTCACCTGATATAGGGCTTGTATATACTGATTATACTTTAATCGATGAGCAAGGTAGTATAG
GTGCAAGACTTTATCAAGAACCGCCGGAGTTTTTACCGATTAGAGATTGTGTCGGTGCATGTTTCTTATATAGAGCCGAT
TTAGCAAGACAAATAGGTGGATATAATGAAAATATGAAGTTGGTGGATGATTATGAATATTGGCTTCGTTTTGGACTTGT
TACGAAATTTGCTCATATACCGGAATCTTTATATTTCTACCGAGTACATGATCAAAGCTTAACAATAGAGCGTAAAGTAG
AAGCAAAACAGAGCAAAAGAGCTTTAAAAGAATTATTTAAAGATAAATACATTATAGCTGATAAGATTAAACCTATAAAT
GATTTATATAATTGGTTTATTGAAGATAGAAATTTAATCTCTTATTTTAGGTTATTTAAAATAATTATTTGTAGTCCGAT
AATTACTATTTCTTATATTATTAAAAATCTGAAAAGAATTAGGTGA

Upstream 100 bases:

>100_bases
TATGTAATACATTATTAAACTTTGAGAATAATGATTATTCCGACGAATTACCAGAAACCGTAATTCAATATACAGAAAAA
CTTGATATTTCTAGAGGTTG

Downstream 100 bases:

>100_bases
TATTTTAGAATAGTGTCATACCGTAGCGGTATCATTGCGCGTATATTAATCGTCATTGCGAGGAGCGAAGCGACGCGGCA
ATCCAGAAAAAATAACAAAA

Product: glycosyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MRINTLKISIVLPVYNREKLLSFAIESCLNQSFKDFELIIIDDCSKDSSVIVARKYAEQDSRIKVIVNETNKTLPASLNI
AFKEAKGEYFTWTSDDNLFHENALEKMVKILDNSPDIGLVYTDYTLIDEQGSIGARLYQEPPEFLPIRDCVGACFLYRAD
LARQIGGYNENMKLVDDYEYWLRFGLVTKFAHIPESLYFYRVHDQSLTIERKVEAKQSKRALKELFKDKYIIADKIKPIN
DLYNWFIEDRNLISYFRLFKIIICSPIITISYIIKNLKRIR

Sequences:

>Translated_281_residues
MRINTLKISIVLPVYNREKLLSFAIESCLNQSFKDFELIIIDDCSKDSSVIVARKYAEQDSRIKVIVNETNKTLPASLNI
AFKEAKGEYFTWTSDDNLFHENALEKMVKILDNSPDIGLVYTDYTLIDEQGSIGARLYQEPPEFLPIRDCVGACFLYRAD
LARQIGGYNENMKLVDDYEYWLRFGLVTKFAHIPESLYFYRVHDQSLTIERKVEAKQSKRALKELFKDKYIIADKIKPIN
DLYNWFIEDRNLISYFRLFKIIICSPIITISYIIKNLKRIR
>Mature_281_residues
MRINTLKISIVLPVYNREKLLSFAIESCLNQSFKDFELIIIDDCSKDSSVIVARKYAEQDSRIKVIVNETNKTLPASLNI
AFKEAKGEYFTWTSDDNLFHENALEKMVKILDNSPDIGLVYTDYTLIDEQGSIGARLYQEPPEFLPIRDCVGACFLYRAD
LARQIGGYNENMKLVDDYEYWLRFGLVTKFAHIPESLYFYRVHDQSLTIERKVEAKQSKRALKELFKDKYIIADKIKPIN
DLYNWFIEDRNLISYFRLFKIIICSPIITISYIIKNLKRIR

Specific function: May be involved in the production of the exopolysaccharide (EPS) component of the extracellular matrix during biofilm formation. EPS is responsible for the adhesion of chains of cells into bundles. Required for biofilm maintenance [H]

COG id: COG0463

COG function: function code M; Glycosyltransferases involved in cell wall biogenesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1788372, Length=104, Percent_Identity=39.4230769230769, Blast_Score=67, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001173 [H]

Pfam domain/function: PF00535 Glycos_transf_2 [H]

EC number: NA

Molecular weight: Translated: 32934; Mature: 32934

Theoretical pI: Translated: 7.83; Mature: 7.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRINTLKISIVLPVYNREKLLSFAIESCLNQSFKDFELIIIDDCSKDSSVIVARKYAEQD
CCEEEEEEEEEEECCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCEEEEEECCCCC
SRIKVIVNETNKTLPASLNIAFKEAKGEYFTWTSDDNLFHENALEKMVKILDNSPDIGLV
CEEEEEEECCCCCCCCEEEEEEEECCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEE
YTDYTLIDEQGSIGARLYQEPPEFLPIRDCVGACFLYRADLARQIGGYNENMKLVDDYEY
EECEEEEECCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHH
WLRFGLVTKFAHIPESLYFYRVHDQSLTIERKVEAKQSKRALKELFKDKYIIADKIKPIN
HHHHHHHHHHHHCCCCEEEEEEECCCEEEHHHHHHHHHHHHHHHHHHCCEEEECCCCCHH
DLYNWFIEDRNLISYFRLFKIIICSPIITISYIIKNLKRIR
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRINTLKISIVLPVYNREKLLSFAIESCLNQSFKDFELIIIDDCSKDSSVIVARKYAEQD
CCEEEEEEEEEEECCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCEEEEEECCCCC
SRIKVIVNETNKTLPASLNIAFKEAKGEYFTWTSDDNLFHENALEKMVKILDNSPDIGLV
CEEEEEEECCCCCCCCEEEEEEEECCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEE
YTDYTLIDEQGSIGARLYQEPPEFLPIRDCVGACFLYRADLARQIGGYNENMKLVDDYEY
EECEEEEECCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHH
WLRFGLVTKFAHIPESLYFYRVHDQSLTIERKVEAKQSKRALKELFKDKYIIADKIKPIN
HHHHHHHHHHHHCCCCEEEEEEECCCEEEHHHHHHHHHHHHHHHHHHCCEEEECCCCCHH
DLYNWFIEDRNLISYFRLFKIIICSPIITISYIIKNLKRIR
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969506; 9384377 [H]