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The map label for this gene is dnaN [H]

Identifier: 157825704

GI number: 157825704

Start: 572240

End: 573379

Strand: Direct

Name: dnaN [H]

Synonym: A1C_03150

Alternate gene names: 157825704

Gene position: 572240-573379 (Clockwise)

Preceding gene: 157825703

Following gene: 157825705

Centisome position: 46.48

GC content: 30.79

Gene sequence:

>1140_bases
ATGTTAAAATTAATAGTTGAAACAAAAACGTTAGTTCAGTCCCTAGGGTTTGCAAGTTCCGTTGTTGAAAAACGTAATGT
AATACCTGAATATGCAAACATTAAATTATCGGCAAAAGACGGCAATCTAGAGCTTAGTTCTACTAATATGGACTTGTACT
TAAGTCAAAAAATAGCAGTACAGGTAGTAAGTGAAGGTGAACTTACCGTTTCTACGAAAACTCTAAATGATATAGTCCGC
AAACTTCCGGATTCTGAGCTTATATTAACAGATCTCGGTACAACGGGACTTGAAATCAAAGGAAAAAATTGTAAATTTAA
TTTATTTACTTTGCCTGTTAGTTCTTTTCCTACAATGGATAGTATTAATCCTGAAGCAAGTTTTAAAATTTCCTGCACGG
ATTTTGCTAAAATAATCGAATCGACAAAATTTTCGATTTCTTTAGATGAAACTCGTTATAATTTAAACGGTGTTTATTTA
CACATAAAAGACAAAGAGTTTTGTTCGGCAAGTACCGATGGGCATAGGCTTTCAATTTCATGGGTAACATTAGAAAAACA
AATAAAGAATTTTGGAGTTATATTGCCGCAAAAAAGTGCAGAAGAAATTTTAAAAATAGTTAAAGATCCTAAAAATATAA
ATGAAGATATAGAAATGTTATTAAGTAGCAATAAGATTAAATTTATATGTAACGAAAATACTATTATGCTATCAAAGCTT
ATAGACGGTACTTTCCCTGATTATAGTGCTTTTATTCCAGAAAGCAGCAGCTCAAAATTAGTAATTAATCGAAAAATATT
TGCAGATAGTATAGAAAGAATAGCGATAATAACCGTTGAAAAATTTAGAGCAGTAAAATTATCATTATCTCGTGAAACCT
TAGAGATTAGTGCTGTCGGTGAAGCAAGAGGTAACGCAAAAGAGGTTATTAATTCTTCACAAGATAAGGAAAGTTTTTAT
GAATATAATAGTGATGAGTCTTTAGTTATAGGCTTTAATCCACAATATTTAGAAGATGTCTTAAAAGCAGTGAAATCGGA
TTTAGTAGAACTATATTTTTCAGATGTTTCAGCACCAGTACTTATTAAATTTCCTGAGAATCCTAAAGATATTTTTGTCG
TCATGCCTGTTAAGGTGTAG

Upstream 100 bases:

>100_bases
GATGATCGTACGTTGTCTGATACTGATTTAAATTCGTTCAGTAAAGATGTAGTTGCTGCCATCTCGAAAAAATTTCAAGG
AACATTAAGAGAATAACAAT

Downstream 100 bases:

>100_bases
AGTAGCTATATGTTATTCATGCAAGGCATTATTACGTGGATCAAAAAGCACACTCGATGTCATCCCATGGCTTGACCACG
GGATCCAACAAAACACTTAA

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 379; Mature: 379

Protein sequence:

>379_residues
MLKLIVETKTLVQSLGFASSVVEKRNVIPEYANIKLSAKDGNLELSSTNMDLYLSQKIAVQVVSEGELTVSTKTLNDIVR
KLPDSELILTDLGTTGLEIKGKNCKFNLFTLPVSSFPTMDSINPEASFKISCTDFAKIIESTKFSISLDETRYNLNGVYL
HIKDKEFCSASTDGHRLSISWVTLEKQIKNFGVILPQKSAEEILKIVKDPKNINEDIEMLLSSNKIKFICNENTIMLSKL
IDGTFPDYSAFIPESSSSKLVINRKIFADSIERIAIITVEKFRAVKLSLSRETLEISAVGEARGNAKEVINSSQDKESFY
EYNSDESLVIGFNPQYLEDVLKAVKSDLVELYFSDVSAPVLIKFPENPKDIFVVMPVKV

Sequences:

>Translated_379_residues
MLKLIVETKTLVQSLGFASSVVEKRNVIPEYANIKLSAKDGNLELSSTNMDLYLSQKIAVQVVSEGELTVSTKTLNDIVR
KLPDSELILTDLGTTGLEIKGKNCKFNLFTLPVSSFPTMDSINPEASFKISCTDFAKIIESTKFSISLDETRYNLNGVYL
HIKDKEFCSASTDGHRLSISWVTLEKQIKNFGVILPQKSAEEILKIVKDPKNINEDIEMLLSSNKIKFICNENTIMLSKL
IDGTFPDYSAFIPESSSSKLVINRKIFADSIERIAIITVEKFRAVKLSLSRETLEISAVGEARGNAKEVINSSQDKESFY
EYNSDESLVIGFNPQYLEDVLKAVKSDLVELYFSDVSAPVLIKFPENPKDIFVVMPVKV
>Mature_379_residues
MLKLIVETKTLVQSLGFASSVVEKRNVIPEYANIKLSAKDGNLELSSTNMDLYLSQKIAVQVVSEGELTVSTKTLNDIVR
KLPDSELILTDLGTTGLEIKGKNCKFNLFTLPVSSFPTMDSINPEASFKISCTDFAKIIESTKFSISLDETRYNLNGVYL
HIKDKEFCSASTDGHRLSISWVTLEKQIKNFGVILPQKSAEEILKIVKDPKNINEDIEMLLSSNKIKFICNENTIMLSKL
IDGTFPDYSAFIPESSSSKLVINRKIFADSIERIAIITVEKFRAVKLSLSRETLEISAVGEARGNAKEVINSSQDKESFY
EYNSDESLVIGFNPQYLEDVLKAVKSDLVELYFSDVSAPVLIKFPENPKDIFVVMPVKV

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=379, Percent_Identity=29.287598944591, Blast_Score=185, Evalue=4e-48,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 42327; Mature: 42327

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKLIVETKTLVQSLGFASSVVEKRNVIPEYANIKLSAKDGNLELSSTNMDLYLSQKIAV
CEEEEEEHHHHHHHCCHHHHHHHHHCCCCCHHCEEEEECCCCEEEECCCEEEEEECEEEE
QVVSEGELTVSTKTLNDIVRKLPDSELILTDLGTTGLEIKGKNCKFNLFTLPVSSFPTMD
EEEECCCEEEEHHHHHHHHHHCCCCCEEEEECCCCCEEEECCCEEEEEEEECCCCCCCCC
SINPEASFKISCTDFAKIIESTKFSISLDETRYNLNGVYLHIKDKEFCSASTDGHRLSIS
CCCCCCEEEEEHHHHHHHHHCCCEEEEEECCEEECCEEEEEEECHHHCCCCCCCCEEEEE
WVTLEKQIKNFGVILPQKSAEEILKIVKDPKNINEDIEMLLSSNKIKFICNENTIMLSKL
EEEEHHHHHHCCEEECCCCHHHHHHHHCCCCCCCHHHHHHHCCCCEEEEECCCEEEEEEH
IDGTFPDYSAFIPESSSSKLVINRKIFADSIERIAIITVEKFRAVKLSLSRETLEISAVG
HCCCCCCCCEECCCCCCCEEEEEEEHHHCCCCEEEEEEEECEEEEEEEECCCEEEEEEEC
EARGNAKEVINSSQDKESFYEYNSDESLVIGFNPQYLEDVLKAVKSDLVELYFSDVSAPV
CCCCCHHHHHCCCCCHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCE
LIKFPENPKDIFVVMPVKV
EEECCCCCCEEEEEEEECC
>Mature Secondary Structure
MLKLIVETKTLVQSLGFASSVVEKRNVIPEYANIKLSAKDGNLELSSTNMDLYLSQKIAV
CEEEEEEHHHHHHHCCHHHHHHHHHCCCCCHHCEEEEECCCCEEEECCCEEEEEECEEEE
QVVSEGELTVSTKTLNDIVRKLPDSELILTDLGTTGLEIKGKNCKFNLFTLPVSSFPTMD
EEEECCCEEEEHHHHHHHHHHCCCCCEEEEECCCCCEEEECCCEEEEEEEECCCCCCCCC
SINPEASFKISCTDFAKIIESTKFSISLDETRYNLNGVYLHIKDKEFCSASTDGHRLSIS
CCCCCCEEEEEHHHHHHHHHCCCEEEEEECCEEECCEEEEEEECHHHCCCCCCCCEEEEE
WVTLEKQIKNFGVILPQKSAEEILKIVKDPKNINEDIEMLLSSNKIKFICNENTIMLSKL
EEEEHHHHHHCCEEECCCCHHHHHHHHCCCCCCCHHHHHHHCCCCEEEEECCCEEEEEEH
IDGTFPDYSAFIPESSSSKLVINRKIFADSIERIAIITVEKFRAVKLSLSRETLEISAVG
HCCCCCCCCEECCCCCCCEEEEEEEHHHCCCCEEEEEEEECEEEEEEEECCCEEEEEEEC
EARGNAKEVINSSQDKESFYEYNSDESLVIGFNPQYLEDVLKAVKSDLVELYFSDVSAPV
CCCCCHHHHHCCCCCHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCE
LIKFPENPKDIFVVMPVKV
EEECCCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]