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The map label for this gene is murD [H]

Identifier: 157825688

GI number: 157825688

Start: 553916

End: 555394

Strand: Direct

Name: murD [H]

Synonym: A1C_03050

Alternate gene names: 157825688

Gene position: 553916-555394 (Clockwise)

Preceding gene: 157825687

Following gene: 157825689

Centisome position: 45.0

GC content: 31.58

Gene sequence:

>1479_bases
ATGCATTCTAATACAAAACAAAAAATAGGTGTTTTTGGGCTTGGTAAAACCGGTATGTCGGTTTATGAAGAGCTGCATGG
TAAATGTGACATAATTATTTATGACGATTTAAAAGCAAATAGAGATATATTTGAAAAATTATATAGTAAAAGTGCTATTG
TTGCTTTATCTGATTTAAGATGGCAAAATTTAGATAAAATTATCTTAAGCCCTGGAGTTCCTTTAACACATAAGATAGTA
GGTATTGCAAAAAATTTTAATATTCCAGTCATTTCTGATATAGATTTATTATTTGAAAAATCAAAAAATTTAAACTTTAT
CGCCATAACTGGTACAAACGGTAAAAGTACTACTACTGCTTTAATAAGCCATATCTTAAATAGTAGCGGTTTAGACTATC
AGGTTGCCGGTAATATTGGAGTTCCTGCTTTGCAAGCTAAAGCAAGTAAGGACGGATATGTACTTGAATTATCTTCTTTT
CAGCTAGATCTAGTAAAAATCTTTACAGCTAAAATAGCAGTGCTTCTTAACATAACTCCAGATCATTTAGACAGACATCA
GCATATGAACGGCTATATTGCAGCAAAATCCAAGATTTTTGATCGAATGGATAAAGATAGTTATGCAGTAATTAATATTG
ATAATGATTATTGTCGTGAAATTTTTATCACATTACAGCAGGAGCAACGTATAAAACTAGTTCCTTTTTCGGTTACTAAA
ATTCTTGAAAACTGCATTTCGGTAGTTGATAATAAAATTAACGATAATTTTTTTGATGATATAAGGTTTGAATGTCAGTA
TAATTCAGAAAGCTTCAGACAAAATGAATTTCAAGGCGAGCCTGCGGAGCGTATAAAAGTATGTGAGCACAGGCTGGACC
TACAAAATTCGCTTGTATCAAATTTTCTGAATGATGCTGTACCTTTTAATAACAATTTGCAAGGAACTCATAATGATGAG
AATATCGCTGCAAGTTATGCAGTAGCTAAAATAATAGGAGTAGAACCTAAAAAAATACTTGAGTCTATAAGTGGTTTTCA
GAGTTTACCGCATAGAATGCAATATATCTGTAGTATAAATAATATAAATTTTTACAATGATAGTAAAGCGACAAATGCTA
TATCTGCCGTACAATCAATTAAAGCACTTGATAATATTTACTGGCTTGCTGGAGGAATCCCTAAAGATGGCGGTATTGAA
TGCATAAAGCCTTATTTCAGTAAAATTAAGAAAGCTTATTTTTATGGTCAAGCTAAAGAAATATTTGCAAATACTACTAA
GAATATAGTAGATTTTGTAATATGTGATAATCTTGAGCAGGCTTTTGATCTTGCTTATAAGGATGCAGTAGGTGATAATG
CGGAGTTGAAAAATATCTTGTTAGCACCTAGTTGTAGCTCATATGATCAGTTTAAAAATTTTGCAGAGCGTGGCGAGTTG
TTTATAAAGTTGTGTAGTATACTATCACCCGTGGCTTGA

Upstream 100 bases:

>100_bases
TGAAAAGGCTTTAATGCAGGATATGGATTAAGAATTGTATTATCCCGTAGCCCCATCCACGGGATGACAATTTTGCGCTC
ACCATATCAATTACAAATTT

Downstream 100 bases:

>100_bases
CTGATAGTACCGGACGGTTGTTAAAAAATTTTCGATGTCATAGCCGCGAAAGCTGGGAGCCAGACTAAAAAATCTTTAAT
TTTAAAGACTTTTTATTAGA

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase [H]

Number of amino acids: Translated: 492; Mature: 492

Protein sequence:

>492_residues
MHSNTKQKIGVFGLGKTGMSVYEELHGKCDIIIYDDLKANRDIFEKLYSKSAIVALSDLRWQNLDKIILSPGVPLTHKIV
GIAKNFNIPVISDIDLLFEKSKNLNFIAITGTNGKSTTTALISHILNSSGLDYQVAGNIGVPALQAKASKDGYVLELSSF
QLDLVKIFTAKIAVLLNITPDHLDRHQHMNGYIAAKSKIFDRMDKDSYAVINIDNDYCREIFITLQQEQRIKLVPFSVTK
ILENCISVVDNKINDNFFDDIRFECQYNSESFRQNEFQGEPAERIKVCEHRLDLQNSLVSNFLNDAVPFNNNLQGTHNDE
NIAASYAVAKIIGVEPKKILESISGFQSLPHRMQYICSINNINFYNDSKATNAISAVQSIKALDNIYWLAGGIPKDGGIE
CIKPYFSKIKKAYFYGQAKEIFANTTKNIVDFVICDNLEQAFDLAYKDAVGDNAELKNILLAPSCSSYDQFKNFAERGEL
FIKLCSILSPVA

Sequences:

>Translated_492_residues
MHSNTKQKIGVFGLGKTGMSVYEELHGKCDIIIYDDLKANRDIFEKLYSKSAIVALSDLRWQNLDKIILSPGVPLTHKIV
GIAKNFNIPVISDIDLLFEKSKNLNFIAITGTNGKSTTTALISHILNSSGLDYQVAGNIGVPALQAKASKDGYVLELSSF
QLDLVKIFTAKIAVLLNITPDHLDRHQHMNGYIAAKSKIFDRMDKDSYAVINIDNDYCREIFITLQQEQRIKLVPFSVTK
ILENCISVVDNKINDNFFDDIRFECQYNSESFRQNEFQGEPAERIKVCEHRLDLQNSLVSNFLNDAVPFNNNLQGTHNDE
NIAASYAVAKIIGVEPKKILESISGFQSLPHRMQYICSINNINFYNDSKATNAISAVQSIKALDNIYWLAGGIPKDGGIE
CIKPYFSKIKKAYFYGQAKEIFANTTKNIVDFVICDNLEQAFDLAYKDAVGDNAELKNILLAPSCSSYDQFKNFAERGEL
FIKLCSILSPVA
>Mature_492_residues
MHSNTKQKIGVFGLGKTGMSVYEELHGKCDIIIYDDLKANRDIFEKLYSKSAIVALSDLRWQNLDKIILSPGVPLTHKIV
GIAKNFNIPVISDIDLLFEKSKNLNFIAITGTNGKSTTTALISHILNSSGLDYQVAGNIGVPALQAKASKDGYVLELSSF
QLDLVKIFTAKIAVLLNITPDHLDRHQHMNGYIAAKSKIFDRMDKDSYAVINIDNDYCREIFITLQQEQRIKLVPFSVTK
ILENCISVVDNKINDNFFDDIRFECQYNSESFRQNEFQGEPAERIKVCEHRLDLQNSLVSNFLNDAVPFNNNLQGTHNDE
NIAASYAVAKIIGVEPKKILESISGFQSLPHRMQYICSINNINFYNDSKATNAISAVQSIKALDNIYWLAGGIPKDGGIE
CIKPYFSKIKKAYFYGQAKEIFANTTKNIVDFVICDNLEQAFDLAYKDAVGDNAELKNILLAPSCSSYDQFKNFAERGEL
FIKLCSILSPVA

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) [H]

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RPE3 insert domain [H]

Homologues:

Organism=Escherichia coli, GI1786276, Length=488, Percent_Identity=28.4836065573771, Blast_Score=155, Evalue=6e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR022437
- InterPro:   IPR005762 [H]

Pfam domain/function: PF08245 Mur_ligase_M [H]

EC number: =6.3.2.9 [H]

Molecular weight: Translated: 55110; Mature: 55110

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00012 PHOSPHOPANTETHEINE ; PS01011 FOLYLPOLYGLU_SYNT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHSNTKQKIGVFGLGKTGMSVYEELHGKCDIIIYDDLKANRDIFEKLYSKSAIVALSDLR
CCCCCCCCEEEEEECCCHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHCCCCEEEEECCC
WQNLDKIILSPGVPLTHKIVGIAKNFNIPVISDIDLLFEKSKNLNFIAITGTNGKSTTTA
CCCCCCEEECCCCCHHHHHEEEHCCCCCCEEECHHHHHHCCCCCEEEEEECCCCCHHHHH
LISHILNSSGLDYQVAGNIGVPALQAKASKDGYVLELSSFQLDLVKIFTAKIAVLLNITP
HHHHHHCCCCCCEEEECCCCCCCHHCCCCCCCEEEEECCCCHHHHHHHHHHHHEEEECCH
DHLDRHQHMNGYIAAKSKIFDRMDKDSYAVINIDNDYCREIFITLQQEQRIKLVPFSVTK
HHHHHHHCCCCEEEHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHCCCCCEEEECHHHHH
ILENCISVVDNKINDNFFDDIRFECQYNSESFRQNEFQGEPAERIKVCEHRLDLQNSLVS
HHHHHHHHHCCCCCCCCCCCEEEEEEECCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
NFLNDAVPFNNNLQGTHNDENIAASYAVAKIIGVEPKKILESISGFQSLPHRMQYICSIN
HHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHEEEC
NINFYNDSKATNAISAVQSIKALDNIYWLAGGIPKDGGIECIKPYFSKIKKAYFYGQAKE
CCCEECCCHHHHHHHHHHHHHHHHHHEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCHHH
IFANTTKNIVDFVICDNLEQAFDLAYKDAVGDNAELKNILLAPSCSSYDQFKNFAERGEL
HHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHEEECCCCCCHHHHHHHHHHHHH
FIKLCSILSPVA
HHHHHHHHCCCC
>Mature Secondary Structure
MHSNTKQKIGVFGLGKTGMSVYEELHGKCDIIIYDDLKANRDIFEKLYSKSAIVALSDLR
CCCCCCCCEEEEEECCCHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHCCCCEEEEECCC
WQNLDKIILSPGVPLTHKIVGIAKNFNIPVISDIDLLFEKSKNLNFIAITGTNGKSTTTA
CCCCCCEEECCCCCHHHHHEEEHCCCCCCEEECHHHHHHCCCCCEEEEEECCCCCHHHHH
LISHILNSSGLDYQVAGNIGVPALQAKASKDGYVLELSSFQLDLVKIFTAKIAVLLNITP
HHHHHHCCCCCCEEEECCCCCCCHHCCCCCCCEEEEECCCCHHHHHHHHHHHHEEEECCH
DHLDRHQHMNGYIAAKSKIFDRMDKDSYAVINIDNDYCREIFITLQQEQRIKLVPFSVTK
HHHHHHHCCCCEEEHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHCCCCCEEEECHHHHH
ILENCISVVDNKINDNFFDDIRFECQYNSESFRQNEFQGEPAERIKVCEHRLDLQNSLVS
HHHHHHHHHCCCCCCCCCCCEEEEEEECCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
NFLNDAVPFNNNLQGTHNDENIAASYAVAKIIGVEPKKILESISGFQSLPHRMQYICSIN
HHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHEEEC
NINFYNDSKATNAISAVQSIKALDNIYWLAGGIPKDGGIECIKPYFSKIKKAYFYGQAKE
CCCEECCCHHHHHHHHHHHHHHHHHHEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCHHH
IFANTTKNIVDFVICDNLEQAFDLAYKDAVGDNAELKNILLAPSCSSYDQFKNFAERGEL
HHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHEEECCCCCCHHHHHHHHHHHHH
FIKLCSILSPVA
HHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]