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The map label for this gene is proP [H]

Identifier: 157825642

GI number: 157825642

Start: 506954

End: 508285

Strand: Direct

Name: proP [H]

Synonym: A1C_02795

Alternate gene names: 157825642

Gene position: 506954-508285 (Clockwise)

Preceding gene: 157825638

Following gene: 157825648

Centisome position: 41.18

GC content: 31.23

Gene sequence:

>1332_bases
ATGTCTAATTCAGCTTTTTTAGTTGCATTTTTTACAACGATTATCCGTTATTATGATTATGCTCTATTCGGTTTATCGGC
TAGTATTCTTGCACAAAATTTCTTACCTGATATAGAGCAAGATAAACAACTACTCGGTTTTTTTGCAATTCTTAGTGCAG
CCGTTATAGTGCGACCTTTAGGGTCTTTAATTTTTGGATTTATCGGAGATAAATACGGGCGTATTAAATCTATTAAGATA
TCAATGTTTATGAGTAGCATTTCTACCGGATTAATTGCTATTACTCCTTCCTTTGAGATGATAGGTATTTTTGCTACGGT
AATATTAACATTATGTCGCATGTTTTTTTTAGCAAGCCTTGCCGGAGAAGTTGACGGAATAAAAATTTATGTAGCTGAAA
AAACAGGAGATAATCGGAAAAATTTAAATATAGGAATAGTTTCATGCTGTAGCCAAATAGGTGCATTACTTGCGGCTATA
GCTTATTATTATGTTAGTAATTCTAGTATATGGTATTTATGGCGTTTAAATTTCTTTATCGGTACAATATTTGGTTTATT
TGCTATTTTGATAAGAAATTTTTTTAAAGAAAGTAGAGAATTTTTATATTATAAAAGTACTATAGAGCTAAATTTAGAAA
GATTGCGACCAGATGAATTTAAGGAAGAGTATGCGGAGCGTACAAAAGTACGTGAGCACAGACGAATTCCGCAAAATTCG
CTTGTATTAAGCTTTCTAAATGACGCTCGGCAAATTATCAAAGATAATACACTATCCTTTATAATAGCATTACTAATAAA
CGGTGCTATTGGAGGAGTGTATCACTTTTTAGTAATTTTCTTGGGAGTTTTGTTAACAAAAATAGCTTTTGTAAATCATC
CTGAAATAAGATTTATGAATATAGCTCTTACTACTACCTATGGAATATCAGCCGTCTTTGCAGGGCTGCTTGCTGATAAA
ATAAACCCTTTAAAACAGATTACATTGACTGTATTTGCTAGCATTATTGTTGCTTTAGGAATGCAAATAATGTTATATAA
ACAAACATTTAATATGCTCTGTCCCGTTATATTAATAGGGCTTGCAGCATTTTATGCAGTACCGCTACAAGTTATTGTTC
AATCTTTGTTTAAGACTAATATTAAAATGAGGCTTTATAGTCTATCTCATTCTTTTGGAGGTATTATTCTATCCTCCACT
ACTCCTTTCTTTAGTATGTTGTTGTGGCAAAATTTTAAGTCACTATCTCTAACTTTAAGCTTGTTCATATTTTTGCTTAT
AATATTAATGAGTACAGTGTTAGTTTTACGTAAGATGTTATCCTTGCATTAA

Upstream 100 bases:

>100_bases
ACAATTAAAACTACAATAGTAAAATATATTAAATTAATTTTCTGTCACACAGCATAAAGAGTTTGTATCATATTATGTTG
TCACCAAAAGCAAATATTAT

Downstream 100 bases:

>100_bases
TACTAAATCGTCATTGTGAGCAACTAAAGGTTTTATTACATGGCTTAATTTCATCTCTGTCATCTCGTGGTTTAACCCAC
TGGATCCAGTTAAAAATACT

Product: proline/betaine transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 443; Mature: 442

Protein sequence:

>443_residues
MSNSAFLVAFFTTIIRYYDYALFGLSASILAQNFLPDIEQDKQLLGFFAILSAAVIVRPLGSLIFGFIGDKYGRIKSIKI
SMFMSSISTGLIAITPSFEMIGIFATVILTLCRMFFLASLAGEVDGIKIYVAEKTGDNRKNLNIGIVSCCSQIGALLAAI
AYYYVSNSSIWYLWRLNFFIGTIFGLFAILIRNFFKESREFLYYKSTIELNLERLRPDEFKEEYAERTKVREHRRIPQNS
LVLSFLNDARQIIKDNTLSFIIALLINGAIGGVYHFLVIFLGVLLTKIAFVNHPEIRFMNIALTTTYGISAVFAGLLADK
INPLKQITLTVFASIIVALGMQIMLYKQTFNMLCPVILIGLAAFYAVPLQVIVQSLFKTNIKMRLYSLSHSFGGIILSST
TPFFSMLLWQNFKSLSLTLSLFIFLLIILMSTVLVLRKMLSLH

Sequences:

>Translated_443_residues
MSNSAFLVAFFTTIIRYYDYALFGLSASILAQNFLPDIEQDKQLLGFFAILSAAVIVRPLGSLIFGFIGDKYGRIKSIKI
SMFMSSISTGLIAITPSFEMIGIFATVILTLCRMFFLASLAGEVDGIKIYVAEKTGDNRKNLNIGIVSCCSQIGALLAAI
AYYYVSNSSIWYLWRLNFFIGTIFGLFAILIRNFFKESREFLYYKSTIELNLERLRPDEFKEEYAERTKVREHRRIPQNS
LVLSFLNDARQIIKDNTLSFIIALLINGAIGGVYHFLVIFLGVLLTKIAFVNHPEIRFMNIALTTTYGISAVFAGLLADK
INPLKQITLTVFASIIVALGMQIMLYKQTFNMLCPVILIGLAAFYAVPLQVIVQSLFKTNIKMRLYSLSHSFGGIILSST
TPFFSMLLWQNFKSLSLTLSLFIFLLIILMSTVLVLRKMLSLH
>Mature_442_residues
SNSAFLVAFFTTIIRYYDYALFGLSASILAQNFLPDIEQDKQLLGFFAILSAAVIVRPLGSLIFGFIGDKYGRIKSIKIS
MFMSSISTGLIAITPSFEMIGIFATVILTLCRMFFLASLAGEVDGIKIYVAEKTGDNRKNLNIGIVSCCSQIGALLAAIA
YYYVSNSSIWYLWRLNFFIGTIFGLFAILIRNFFKESREFLYYKSTIELNLERLRPDEFKEEYAERTKVREHRRIPQNSL
VLSFLNDARQIIKDNTLSFIIALLINGAIGGVYHFLVIFLGVLLTKIAFVNHPEIRFMNIALTTTYGISAVFAGLLADKI
NPLKQITLTVFASIIVALGMQIMLYKQTFNMLCPVILIGLAAFYAVPLQVIVQSLFKTNIKMRLYSLSHSFGGIILSSTT
PFFSMLLWQNFKSLSLTLSLFIFLLIILMSTVLVLRKMLSLH

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=229, Percent_Identity=29.2576419213974, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1788292, Length=206, Percent_Identity=29.126213592233, Blast_Score=98, Evalue=9e-22,
Organism=Escherichia coli, GI1788942, Length=193, Percent_Identity=26.9430051813472, Blast_Score=69, Evalue=9e-13,
Organism=Escherichia coli, GI1789941, Length=212, Percent_Identity=25.4716981132075, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 49899; Mature: 49768

Theoretical pI: Translated: 9.92; Mature: 9.92

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNSAFLVAFFTTIIRYYDYALFGLSASILAQNFLPDIEQDKQLLGFFAILSAAVIVRPL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
GSLIFGFIGDKYGRIKSIKISMFMSSISTGLIAITPSFEMIGIFATVILTLCRMFFLASL
HHHHHHHHCCCCCCCHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHH
AGEVDGIKIYVAEKTGDNRKNLNIGIVSCCSQIGALLAAIAYYYVSNSSIWYLWRLNFFI
HCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHH
GTIFGLFAILIRNFFKESREFLYYKSTIELNLERLRPDEFKEEYAERTKVREHRRIPQNS
HHHHHHHHHHHHHHHHHHHHHEEEEHHHEEEHHHCCCHHHHHHHHHHHHHHHHHCCCCHH
LVLSFLNDARQIIKDNTLSFIIALLINGAIGGVYHFLVIFLGVLLTKIAFVNHPEIRFMN
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
IALTTTYGISAVFAGLLADKINPLKQITLTVFASIIVALGMQIMLYKQTFNMLCPVILIG
EEEEHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAAFYAVPLQVIVQSLFKTNIKMRLYSLSHSFGGIILSSTTPFFSMLLWQNFKSLSLTLS
HHHHHHHHHHHHHHHHHHCCHHHEEEEHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHH
LFIFLLIILMSTVLVLRKMLSLH
HHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SNSAFLVAFFTTIIRYYDYALFGLSASILAQNFLPDIEQDKQLLGFFAILSAAVIVRPL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
GSLIFGFIGDKYGRIKSIKISMFMSSISTGLIAITPSFEMIGIFATVILTLCRMFFLASL
HHHHHHHHCCCCCCCHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHH
AGEVDGIKIYVAEKTGDNRKNLNIGIVSCCSQIGALLAAIAYYYVSNSSIWYLWRLNFFI
HCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHH
GTIFGLFAILIRNFFKESREFLYYKSTIELNLERLRPDEFKEEYAERTKVREHRRIPQNS
HHHHHHHHHHHHHHHHHHHHHEEEEHHHEEEHHHCCCHHHHHHHHHHHHHHHHHCCCCHH
LVLSFLNDARQIIKDNTLSFIIALLINGAIGGVYHFLVIFLGVLLTKIAFVNHPEIRFMN
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
IALTTTYGISAVFAGLLADKINPLKQITLTVFASIIVALGMQIMLYKQTFNMLCPVILIG
EEEEHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAAFYAVPLQVIVQSLFKTNIKMRLYSLSHSFGGIILSSTTPFFSMLLWQNFKSLSLTLS
HHHHHHHHHHHHHHHHHHCCHHHEEEEHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHH
LFIFLLIILMSTVLVLRKMLSLH
HHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]