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The map label for this gene is hslU [H]

Identifier: 157825563

GI number: 157825563

Start: 435107

End: 436459

Strand: Direct

Name: hslU [H]

Synonym: A1C_02370

Alternate gene names: 157825563

Gene position: 435107-436459 (Clockwise)

Preceding gene: 157825562

Following gene: 157825564

Centisome position: 35.34

GC content: 34.59

Gene sequence:

>1353_bases
ATGAAAGCTACTAAAACTACTTATCAAAAAGACCATATGGGGCTTACACCTTCTCAAATAGTTAATGAACTTAATAGATT
TATCGTAGGTCAAGAAAAGGCCAAAAAAGCTGTTGCTATTGCACTTAGAAACCGCTGTCGTCGTAAAAGAGTAGAAGGTA
ATTTACGTAATGAAATAGTACCGAAAAATATTTTAATGATTGGTTCAACCGGTGTCGGAAAAACGGAAATAGCAAGACGC
CTTGCAAAGTTGACTTATTCTCCTTTCTATAAGATAGAAGCAACTAAATTTACTGAAGTCGGATATGTAGGGCGTGATGT
AGAATCAATAATTCGTGATTTAGTTGAAATAGCTGTTAATACTGAAAAAACTTTAGCAAAAACAGAAGTAGATATTAATG
CCCGTGAGCAAGCGATAGAGAGAATATTAGATAGTTTAGTAGGTAAAACTTCTAGTAGTGAGACTAGAGAAAAGTTCAAA
GAAAAAATTTTAAACGGTGAACTTGATGATACAGAAATCGAAATTAGCGTAGCTGATACTACACCTGTCGGTGGTGGAAG
TTTTGAAATACCGGGTATGCCAGGAGCATCTATGGGCGTTCTTAACCTCGGTGACGTGATTGGACGAGCTCTTGGCGGCA
GTAAGACTAAAACAAAAAAAATGCTAGTTAAAGATGCTATGGCTATTATTATACCTGAGGAATCAGAAAAATTAATAGAC
CAAGAAAAAATTATTCAGCAAGCTATAAATTTAGCTGAAAATGACGGGATAGTTTTTATTGACGAAATTGATAAAATAGC
TTCGACCGGTAGTTCCGGAGCAAAAAATGCCGAAATAAGTAGAGAGGGTGTACAAAGAGATTTGCTACCTTTGATAGAAG
GAACAACGGTTAATACTAAATATGGACCGGTTAAAACAGATCATATATTATTTATTGCTTCAGGTGCTTTCCATATTGCT
AAACCTTCTGATTTATTACCGGAGTTACAAGGAAGGTTACCGATTAGAGTAGAATTAAATTCGCTCACCAAAGATGATAT
GATTAAAATATTGCTTGAACCTGAAACTAGTTTAATAAAGCAATATTCGGCATTAATAGGTACTGAAGACGTGTATATTG
CATTTACGGATTCTGCTATTGAGAAGATAGCGGATTATGCTATCACCGTTAATTTAGAAGTCGAAGATATAGGGGCTAGA
AGGCTGCATACTATACTTGAGAATTTGCTTGAAGATATAAGCTTTGAAGCTAGTGAAATGAAAGGCAGAAAAATAACTAT
CGACGATAAATTCGTAGAAAATCAATTATCAAAAATAATAACTAATCTTGACTTAGCTAAGTTTGTCCTATAA

Upstream 100 bases:

>100_bases
AGAATAATTTAACTGCTGAAGAAATTGCTTTAAAATCTATGAATATAGCTGCAGATCTATGTGTATTTTCTAATCATAAT
ATTATAATGGAAAAAGTTGT

Downstream 100 bases:

>100_bases
TATACTTTTTTAATCATTGATTGAGATAGAATTGTTGTGCATCTCTTTAATGTCATTCCAGTGTAGGATGGAATTCAGGG
TAAAGCGAGCTTTTAGCTTT

Product: ATP-dependent protease ATP-binding subunit HslU

Products: NA

Alternate protein names: Unfoldase HslU [H]

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MKATKTTYQKDHMGLTPSQIVNELNRFIVGQEKAKKAVAIALRNRCRRKRVEGNLRNEIVPKNILMIGSTGVGKTEIARR
LAKLTYSPFYKIEATKFTEVGYVGRDVESIIRDLVEIAVNTEKTLAKTEVDINAREQAIERILDSLVGKTSSSETREKFK
EKILNGELDDTEIEISVADTTPVGGGSFEIPGMPGASMGVLNLGDVIGRALGGSKTKTKKMLVKDAMAIIIPEESEKLID
QEKIIQQAINLAENDGIVFIDEIDKIASTGSSGAKNAEISREGVQRDLLPLIEGTTVNTKYGPVKTDHILFIASGAFHIA
KPSDLLPELQGRLPIRVELNSLTKDDMIKILLEPETSLIKQYSALIGTEDVYIAFTDSAIEKIADYAITVNLEVEDIGAR
RLHTILENLLEDISFEASEMKGRKITIDDKFVENQLSKIITNLDLAKFVL

Sequences:

>Translated_450_residues
MKATKTTYQKDHMGLTPSQIVNELNRFIVGQEKAKKAVAIALRNRCRRKRVEGNLRNEIVPKNILMIGSTGVGKTEIARR
LAKLTYSPFYKIEATKFTEVGYVGRDVESIIRDLVEIAVNTEKTLAKTEVDINAREQAIERILDSLVGKTSSSETREKFK
EKILNGELDDTEIEISVADTTPVGGGSFEIPGMPGASMGVLNLGDVIGRALGGSKTKTKKMLVKDAMAIIIPEESEKLID
QEKIIQQAINLAENDGIVFIDEIDKIASTGSSGAKNAEISREGVQRDLLPLIEGTTVNTKYGPVKTDHILFIASGAFHIA
KPSDLLPELQGRLPIRVELNSLTKDDMIKILLEPETSLIKQYSALIGTEDVYIAFTDSAIEKIADYAITVNLEVEDIGAR
RLHTILENLLEDISFEASEMKGRKITIDDKFVENQLSKIITNLDLAKFVL
>Mature_450_residues
MKATKTTYQKDHMGLTPSQIVNELNRFIVGQEKAKKAVAIALRNRCRRKRVEGNLRNEIVPKNILMIGSTGVGKTEIARR
LAKLTYSPFYKIEATKFTEVGYVGRDVESIIRDLVEIAVNTEKTLAKTEVDINAREQAIERILDSLVGKTSSSETREKFK
EKILNGELDDTEIEISVADTTPVGGGSFEIPGMPGASMGVLNLGDVIGRALGGSKTKTKKMLVKDAMAIIIPEESEKLID
QEKIIQQAINLAENDGIVFIDEIDKIASTGSSGAKNAEISREGVQRDLLPLIEGTTVNTKYGPVKTDHILFIASGAFHIA
KPSDLLPELQGRLPIRVELNSLTKDDMIKILLEPETSLIKQYSALIGTEDVYIAFTDSAIEKIADYAITVNLEVEDIGAR
RLHTILENLLEDISFEASEMKGRKITIDDKFVENQLSKIITNLDLAKFVL

Specific function: ATPase subunit of a proteasome-like degradation complex; this subunit has chaperone activity. The binding of ATP and its subsequent hydrolysis by HslU are essential for unfolding of protein substrates subsequently hydrolyzed by HslV. HslU recognizes the N

COG id: COG1220

COG function: function code O; ATP-dependent protease HslVU (ClpYQ), ATPase subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ClpX chaperone family. HslU subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790366, Length=447, Percent_Identity=54.3624161073826, Blast_Score=454, Evalue=1e-129,
Organism=Escherichia coli, GI1786642, Length=255, Percent_Identity=31.7647058823529, Blast_Score=101, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6319704, Length=124, Percent_Identity=32.258064516129, Blast_Score=67, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24648291, Length=240, Percent_Identity=30, Blast_Score=92, Evalue=6e-19,
Organism=Drosophila melanogaster, GI24648289, Length=241, Percent_Identity=30.2904564315353, Blast_Score=92, Evalue=6e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR019489
- InterPro:   IPR004491 [H]

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF10431 ClpB_D2-small [H]

EC number: NA

Molecular weight: Translated: 49641; Mature: 49641

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKATKTTYQKDHMGLTPSQIVNELNRFIVGQEKAKKAVAIALRNRCRRKRVEGNLRNEIV
CCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHCCC
PKNILMIGSTGVGKTEIARRLAKLTYSPFYKIEATKFTEVGYVGRDVESIIRDLVEIAVN
CCCEEEEECCCCCHHHHHHHHHHHHCCCCEEEEECEEHHCCCCCCCHHHHHHHHHHHHHC
TEKTLAKTEVDINAREQAIERILDSLVGKTSSSETREKFKEKILNGELDDTEIEISVADT
CCHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEECC
TPVGGGSFEIPGMPGASMGVLNLGDVIGRALGGSKTKTKKMLVKDAMAIIIPEESEKLID
CCCCCCCEECCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCEEEEECCCHHHHHH
QEKIIQQAINLAENDGIVFIDEIDKIASTGSSGAKNAEISREGVQRDLLPLIEGTTVNTK
HHHHHHHHHHHCCCCCEEEEECHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCEECCC
YGPVKTDHILFIASGAFHIAKPSDLLPELQGRLPIRVELNSLTKDDMIKILLEPETSLIK
CCCCCCCEEEEEECCCEEECCCHHHHHHHHCCCCEEEEECCCCCCCEEEEEECCCHHHHH
QYSALIGTEDVYIAFTDSAIEKIADYAITVNLEVEDIGARRLHTILENLLEDISFEASEM
HHHHHHCCCCEEEEEEHHHHHHHHCEEEEEEEEEHHCCHHHHHHHHHHHHHHCCCCHHHC
KGRKITIDDKFVENQLSKIITNLDLAKFVL
CCCEEEECHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKATKTTYQKDHMGLTPSQIVNELNRFIVGQEKAKKAVAIALRNRCRRKRVEGNLRNEIV
CCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHCCC
PKNILMIGSTGVGKTEIARRLAKLTYSPFYKIEATKFTEVGYVGRDVESIIRDLVEIAVN
CCCEEEEECCCCCHHHHHHHHHHHHCCCCEEEEECEEHHCCCCCCCHHHHHHHHHHHHHC
TEKTLAKTEVDINAREQAIERILDSLVGKTSSSETREKFKEKILNGELDDTEIEISVADT
CCHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEECC
TPVGGGSFEIPGMPGASMGVLNLGDVIGRALGGSKTKTKKMLVKDAMAIIIPEESEKLID
CCCCCCCEECCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCEEEEECCCHHHHHH
QEKIIQQAINLAENDGIVFIDEIDKIASTGSSGAKNAEISREGVQRDLLPLIEGTTVNTK
HHHHHHHHHHHCCCCCEEEEECHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCEECCC
YGPVKTDHILFIASGAFHIAKPSDLLPELQGRLPIRVELNSLTKDDMIKILLEPETSLIK
CCCCCCCEEEEEECCCEEECCCHHHHHHHHCCCCEEEEECCCCCCCEEEEEECCCHHHHH
QYSALIGTEDVYIAFTDSAIEKIADYAITVNLEVEDIGARRLHTILENLLEDISFEASEM
HHHHHHCCCCEEEEEEHHHHHHHHCEEEEEEEEEHHCCHHHHHHHHHHHHHHCCCCHHHC
KGRKITIDDKFVENQLSKIITNLDLAKFVL
CCCEEEECHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9823893 [H]