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The map label for this gene is rbsR [H]

Identifier: 157373127

GI number: 157373127

Start: 5432463

End: 5433464

Strand: Reverse

Name: rbsR [H]

Synonym: Spro_4896

Alternate gene names: 157373127

Gene position: 5433464-5432463 (Counterclockwise)

Preceding gene: 157373128

Following gene: 157373122

Centisome position: 99.72

GC content: 57.09

Gene sequence:

>1002_bases
TTGGCCACCATGAAAGACGTCGCCCGCCTGGCGGGCGTTTCAACCTCGACGGTCTCGCACGTCATTAATGACAATCGCTT
TGTCAGCGACAGCGTGCGGGCCAAAGTCATGGCGGCCGTTGAACAACTGAACTATGCGCCTTCTGCGCTGGCGCGCAGCC
TCAAACTAAACCAGACGCGTACCATCGGCATGCTGGTTACCTCCAGCAATAACCCGTTTTATGCCGAAGTCGTGCGCGGC
GTTGAGCGCAGTTGCTATGAACGTGGCTACAGTCTGATCCTGTGCAATACCGAAGAAGATGCCGCACGCATGAATCGCAA
CATGGAGACGCTGTTGCAAAAACGCGTCGATGGCTTGCTATTAATGTGCACCGAAAATCATCGGCCATCGCAGGACGCGC
TGAGTCGTTATCCTTCGTTGCCGATCGTCATGATGGATTGGGCGCCGTTCGAAGGGGCCAACGATATCATTCAGGACAAC
TCGCTGCTGGGCGGTGAGATGGCGACCGATCACCTGATCGCCCGTGGGTATCGCAAGATTGCCTGCATCGCCGGCCCACA
GGATAAAACTACCGCACGTCATAGGCTGGAAGGCTACCGTCGCGCCATGCAACGCGCCGGATTGGCAGTCTTGCCGGGGT
ACGAAGTGCACTGCGATTTTGAATTTGAAGGCGGCGTGGATGCCATGAAGCAACTGCTGGCGCTGGCTGAACCCCCGCAT
GCGGTATTCGCCGGTAACGATGCGGTAGCCGTAGGTGCCTATCAGGCTCTGTATCAGGCCGGATTGTCGGTGCCGCAAGA
CATGGCGGTGATGGGCTACGATGATATAGAGCTGGCCAAATATCTGGCCCCACCGTTAAGCACTATTCACCAACCGAAAG
ATTCACTGGGGGAGTTGGCGATTGATACGCTGATTAACCGCCTGCAGAACCCGGACCGCGAAGCCCAGGTTCTGGTGTTG
ACCCCTGAGCTGGTGGAGCGTGCTTCGGTCGGTTACCGTTAA

Upstream 100 bases:

>100_bases
ATGCGGCGGCGGCCATTGCGGTGACCCGTCCAGGTGCTCAGCCTTCCGTACCCTGGCGCGAAGAGATCGACATCTTCTTG
CAGCAGCAGGAGTAATCCCC

Downstream 100 bases:

>100_bases
CCCGCGGTTTTAACCGGTTTCTTCGGGTCCTGGCCGCTGATCAGGTTACGGCCGTCCTTTCTTCTCAGTAACATAAACAC
AAACGCCGAGGCCACGGTGA

Product: transcriptional repressor RbsR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 333; Mature: 332

Protein sequence:

>333_residues
MATMKDVARLAGVSTSTVSHVINDNRFVSDSVRAKVMAAVEQLNYAPSALARSLKLNQTRTIGMLVTSSNNPFYAEVVRG
VERSCYERGYSLILCNTEEDAARMNRNMETLLQKRVDGLLLMCTENHRPSQDALSRYPSLPIVMMDWAPFEGANDIIQDN
SLLGGEMATDHLIARGYRKIACIAGPQDKTTARHRLEGYRRAMQRAGLAVLPGYEVHCDFEFEGGVDAMKQLLALAEPPH
AVFAGNDAVAVGAYQALYQAGLSVPQDMAVMGYDDIELAKYLAPPLSTIHQPKDSLGELAIDTLINRLQNPDREAQVLVL
TPELVERASVGYR

Sequences:

>Translated_333_residues
MATMKDVARLAGVSTSTVSHVINDNRFVSDSVRAKVMAAVEQLNYAPSALARSLKLNQTRTIGMLVTSSNNPFYAEVVRG
VERSCYERGYSLILCNTEEDAARMNRNMETLLQKRVDGLLLMCTENHRPSQDALSRYPSLPIVMMDWAPFEGANDIIQDN
SLLGGEMATDHLIARGYRKIACIAGPQDKTTARHRLEGYRRAMQRAGLAVLPGYEVHCDFEFEGGVDAMKQLLALAEPPH
AVFAGNDAVAVGAYQALYQAGLSVPQDMAVMGYDDIELAKYLAPPLSTIHQPKDSLGELAIDTLINRLQNPDREAQVLVL
TPELVERASVGYR
>Mature_332_residues
ATMKDVARLAGVSTSTVSHVINDNRFVSDSVRAKVMAAVEQLNYAPSALARSLKLNQTRTIGMLVTSSNNPFYAEVVRGV
ERSCYERGYSLILCNTEEDAARMNRNMETLLQKRVDGLLLMCTENHRPSQDALSRYPSLPIVMMDWAPFEGANDIIQDNS
LLGGEMATDHLIARGYRKIACIAGPQDKTTARHRLEGYRRAMQRAGLAVLPGYEVHCDFEFEGGVDAMKQLLALAEPPHA
VFAGNDAVAVGAYQALYQAGLSVPQDMAVMGYDDIELAKYLAPPLSTIHQPKDSLGELAIDTLINRLQNPDREAQVLVLT
PELVERASVGYR

Specific function: Transcriptional repressor for the ribose rbsDACBK operon. RbsR binds to a region of perfect dyad symmetry spanning the rbs operon transcriptional start site. The affinity for the rbs operator is reduced by addition of ribose, consistent with ribose being

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790194, Length=329, Percent_Identity=71.1246200607903, Blast_Score=498, Evalue=1e-142,
Organism=Escherichia coli, GI1787948, Length=331, Percent_Identity=46.2235649546828, Blast_Score=309, Evalue=2e-85,
Organism=Escherichia coli, GI1790369, Length=330, Percent_Identity=33.9393939393939, Blast_Score=207, Evalue=7e-55,
Organism=Escherichia coli, GI1789202, Length=331, Percent_Identity=31.7220543806647, Blast_Score=156, Evalue=2e-39,
Organism=Escherichia coli, GI1787580, Length=325, Percent_Identity=29.2307692307692, Blast_Score=149, Evalue=3e-37,
Organism=Escherichia coli, GI1789068, Length=314, Percent_Identity=29.6178343949045, Blast_Score=144, Evalue=9e-36,
Organism=Escherichia coli, GI1786540, Length=314, Percent_Identity=32.484076433121, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI1788474, Length=336, Percent_Identity=30.0595238095238, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI48994940, Length=309, Percent_Identity=28.8025889967638, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI1787906, Length=332, Percent_Identity=23.4939759036145, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI1790715, Length=311, Percent_Identity=25.7234726688103, Blast_Score=110, Evalue=9e-26,
Organism=Escherichia coli, GI1789456, Length=343, Percent_Identity=26.8221574344023, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI1786268, Length=322, Percent_Identity=26.7080745341615, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI1790192, Length=251, Percent_Identity=28.2868525896414, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1790689, Length=329, Percent_Identity=24.9240121580547, Blast_Score=81, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36630; Mature: 36499

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATMKDVARLAGVSTSTVSHVINDNRFVSDSVRAKVMAAVEQLNYAPSALARSLKLNQTR
CCCHHHHHHHHCCCHHHHHHHHCCCCEEHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCE
TIGMLVTSSNNPFYAEVVRGVERSCYERGYSLILCNTEEDAARMNRNMETLLQKRVDGLL
EEEEEEECCCCCCHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHCCCHHHHHHHHHCCEE
LMCTENHRPSQDALSRYPSLPIVMMDWAPFEGANDIIQDNSLLGGEMATDHLIARGYRKI
EEEECCCCCCHHHHHHCCCCCEEEEECCCCCCCCHHHCCCCCCCCHHHHHHHHHHCCCEE
ACIAGPQDKTTARHRLEGYRRAMQRAGLAVLPGYEVHCDFEFEGGVDAMKQLLALAEPPH
EEEECCCCHHHHHHHHHHHHHHHHHCCEEEECCEEEEEEEEECCCHHHHHHHHHHCCCCC
AVFAGNDAVAVGAYQALYQAGLSVPQDMAVMGYDDIELAKYLAPPLSTIHQPKDSLGELA
EEEECCCEEEHHHHHHHHHHCCCCCCCCEEECCCHHHHHHHHCCCHHHHHCCHHHHHHHH
IDTLINRLQNPDREAQVLVLTPELVERASVGYR
HHHHHHHHCCCCCCCEEEEECHHHHHHHCCCCC
>Mature Secondary Structure 
ATMKDVARLAGVSTSTVSHVINDNRFVSDSVRAKVMAAVEQLNYAPSALARSLKLNQTR
CCHHHHHHHHCCCHHHHHHHHCCCCEEHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCE
TIGMLVTSSNNPFYAEVVRGVERSCYERGYSLILCNTEEDAARMNRNMETLLQKRVDGLL
EEEEEEECCCCCCHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHCCCHHHHHHHHHCCEE
LMCTENHRPSQDALSRYPSLPIVMMDWAPFEGANDIIQDNSLLGGEMATDHLIARGYRKI
EEEECCCCCCHHHHHHCCCCCEEEEECCCCCCCCHHHCCCCCCCCHHHHHHHHHHCCCEE
ACIAGPQDKTTARHRLEGYRRAMQRAGLAVLPGYEVHCDFEFEGGVDAMKQLLALAEPPH
EEEECCCCHHHHHHHHHHHHHHHHHCCEEEECCEEEEEEEEECCCHHHHHHHHHHCCCCC
AVFAGNDAVAVGAYQALYQAGLSVPQDMAVMGYDDIELAKYLAPPLSTIHQPKDSLGELA
EEEECCCEEEHHHHHHHHHHCCCCCCCCEEECCCHHHHHHHHCCCHHHHHCCHHHHHHHH
IDTLINRLQNPDREAQVLVLTPELVERASVGYR
HHHHHHHHCCCCCCCEEEEECHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]