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The map label for this gene is dsbA [H]

Identifier: 157373120

GI number: 157373120

Start: 5421314

End: 5421937

Strand: Reverse

Name: dsbA [H]

Synonym: Spro_4889

Alternate gene names: 157373120

Gene position: 5421937-5421314 (Counterclockwise)

Preceding gene: 157373121

Following gene: 157373118

Centisome position: 99.51

GC content: 50.48

Gene sequence:

>624_bases
ATGAAGAAAATTTGGTTGGCACTCGTTGGCATGGTAATGGCATTCAGTGCCTCGGCTGCGCAATTTAGCGATGGTGCTCA
GTACGTAACCCTGGATAAGCCGGTGACCGGTGAACCTCAGGTACTGGAGTTCTTTTCCTTCTATTGCCCGCACTGCTATC
AGTTCGAGCAGGTGTATCACGTTTCCGAAAATGTGAAAAAAGCGCTGCCTGCGGGCACTAAAATGACCAAGTACCACGTT
GAATTCCTGGGGCCGTTGGGTAAACAACTGACTCAGGCCTGGGCTGTGGCAATGGCGCTGGGCGTGGAAGATAAAGTTAG
CCCGCTGATGTTTGAAGCGGTGCAGAAAACCCAGACCGTGCAAACTCCGGATGATATCCGCAATGTGTTTGTGAAGGCTG
GCGTGAGTGCGGCTGATTATGATGGGGCCTTGAACAGCTTCGTGGTTAAATCCCTGGTGGTTCAACAAGAGAAAGCTGCA
GAAGATCTGCAACTGCGTGGTGTGCCGGCAGTATTTGTTAACGGCAAATACATGGTTAAAAACGATGGCCTGGATACCAG
CTCGATGGATGCTTACGTGAAGCAATTCGCCGAAGTGGTTAAATTCCTCAGCGAGCAGAAGTAA

Upstream 100 bases:

>100_bases
AGACGGCGGCATTCACCGAGCAGGTTAAGCTGTTACAGGAACCCCCTCTGCAGCTGATGCCAATGTATTGAAGCTTTAAC
ACAATGGAGAGTTTAGTCTT

Downstream 100 bases:

>100_bases
CCAACATTTAAAGTGAAAAGCCGGCTTAGCCGGCTTCTTTTACTCTACGATTGCTTCAACTTATCCAGCAGCCGGTCTTT
ATCCTGCCACAGCGTATTCA

Product: periplasmic protein disulfide isomerase I

Products: 3-oxo-D 4-steroid; protein with oxidized disulfide bonds [C]

Alternate protein names: NA

Number of amino acids: Translated: 207; Mature: 207

Protein sequence:

>207_residues
MKKIWLALVGMVMAFSASAAQFSDGAQYVTLDKPVTGEPQVLEFFSFYCPHCYQFEQVYHVSENVKKALPAGTKMTKYHV
EFLGPLGKQLTQAWAVAMALGVEDKVSPLMFEAVQKTQTVQTPDDIRNVFVKAGVSAADYDGALNSFVVKSLVVQQEKAA
EDLQLRGVPAVFVNGKYMVKNDGLDTSSMDAYVKQFAEVVKFLSEQK

Sequences:

>Translated_207_residues
MKKIWLALVGMVMAFSASAAQFSDGAQYVTLDKPVTGEPQVLEFFSFYCPHCYQFEQVYHVSENVKKALPAGTKMTKYHV
EFLGPLGKQLTQAWAVAMALGVEDKVSPLMFEAVQKTQTVQTPDDIRNVFVKAGVSAADYDGALNSFVVKSLVVQQEKAA
EDLQLRGVPAVFVNGKYMVKNDGLDTSSMDAYVKQFAEVVKFLSEQK
>Mature_207_residues
MKKIWLALVGMVMAFSASAAQFSDGAQYVTLDKPVTGEPQVLEFFSFYCPHCYQFEQVYHVSENVKKALPAGTKMTKYHV
EFLGPLGKQLTQAWAVAMALGVEDKVSPLMFEAVQKTQTVQTPDDIRNVFVKAGVSAADYDGALNSFVVKSLVVQQEKAA
EDLQLRGVPAVFVNGKYMVKNDGLDTSSMDAYVKQFAEVVKFLSEQK

Specific function: Required for disulfide bond formation in some periplasmic proteins such as PhoA or OmpA. Acts by transferring its disulfide bond to other proteins and is reduced in the process. DsbA is reoxidized by DsbB. It is required for pilus biogenesis [H]

COG id: COG0526

COG function: function code OC; Thiol-disulfide isomerase and thioredoxins

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 thioredoxin domain [H]

Homologues:

Organism=Escherichia coli, GI1790291, Length=208, Percent_Identity=70.1923076923077, Blast_Score=312, Evalue=9e-87,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 1348 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001853
- InterPro:   IPR023205
- InterPro:   IPR017936
- InterPro:   IPR012336
- InterPro:   IPR017937
- InterPro:   IPR012335 [H]

Pfam domain/function: PF01323 DSBA [H]

EC number: NA

Molecular weight: Translated: 22868; Mature: 22868

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: PS00194 THIOREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIWLALVGMVMAFSASAAQFSDGAQYVTLDKPVTGEPQVLEFFSFYCPHCYQFEQVYH
CHHHHHHHHHHHHHHHCCHHHHCCCCEEEEECCCCCCCHHHHHHHHHHCCCHHHHHHHHH
VSENVKKALPAGTKMTKYHVEFLGPLGKQLTQAWAVAMALGVEDKVSPLMFEAVQKTQTV
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCC
QTPDDIRNVFVKAGVSAADYDGALNSFVVKSLVVQQEKAAEDLQLRGVPAVFVNGKYMVK
CCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCEEEEE
NDGLDTSSMDAYVKQFAEVVKFLSEQK
CCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKKIWLALVGMVMAFSASAAQFSDGAQYVTLDKPVTGEPQVLEFFSFYCPHCYQFEQVYH
CHHHHHHHHHHHHHHHCCHHHHCCCCEEEEECCCCCCCHHHHHHHHHHCCCHHHHHHHHH
VSENVKKALPAGTKMTKYHVEFLGPLGKQLTQAWAVAMALGVEDKVSPLMFEAVQKTQTV
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCC
QTPDDIRNVFVKAGVSAADYDGALNSFVVKSLVVQQEKAAEDLQLRGVPAVFVNGKYMVK
CCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCEEEEE
NDGLDTSSMDAYVKQFAEVVKFLSEQK
CCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-oxo-D 5-steroid; protein with reduced SH groups [C]

Specific reaction: 3-oxo-D 5-steroid = 3-oxo-D 4-steroid protein with reduced SH groups = protein with oxidized disulfide bonds [C]

General reaction: Isomerases; Intramolecular Oxidoreductases; Transposing S-S Bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10438793; 11586360; 12142430 [H]