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The map label for this gene is emrD [H]

Identifier: 157373032

GI number: 157373032

Start: 5319961

End: 5321145

Strand: Reverse

Name: emrD [H]

Synonym: Spro_4800

Alternate gene names: 157373032

Gene position: 5321145-5319961 (Counterclockwise)

Preceding gene: 157373035

Following gene: 157373028

Centisome position: 97.66

GC content: 59.58

Gene sequence:

>1185_bases
ATGAGAAAAATAGAAAACTTTCACCTGTTGGTGATGCTTACCCTGTTAGTGGCCGTCGGTCAGATGGCACAAACCATTTA
TGTCCCCGTGATTGCTGATATTGCCAAGGATCTGTCGGTGCGCTCCGGTGCCGTGCAGCGCGTGATGGCGGCTTATCTGA
TGACCTATGGCTTCTCGCAGTTGATTTACGGCCCGCTGTCGGACCGTATTGGCCGTCGGCCGGTGATCCTGGCCGGGATG
ATGATTTTTATGATGGGTGCGCTTGGCGCGCTGCTGTCCACCAACCTGACGATGCTGGTTGCCGCCAGTGCCATTCAGGG
GATGGGGACCGGCGTGGCGGGGGTGATGGCGCGAACCATGCCGCGTGACCTGTATGCCGGTACTTCGCTGCGCTATGCCA
ACAGCCTGCTCAACATGGGCATTCTGGTCAGTCCGCTGTTGGCACCGATCATCGGTGGGGCATTGGCGATGATATTTGGC
TGGCGCGCCTGTTATGCCTTCCTGTTGGTGTTGTGCGCCGGCGTGGCCTTCGCCATGTTCCGCTGGCTGCCGGAAACCCG
ACCGCAGCAGACCGAAAAACGGCGCATGCTGGCAAGCTTCCGCAAGCTGCTGGCGGACAGCACTTTCAGCTGTTATCTGG
TGATGCTGATCGGTGCTCTGGCGGGGATCGCGGTCTTTGAAGCCAGCTGCGGCGTGCTGATGGGCGGCGTACTCGGCCTT
AGCGGACTGACCGTCAGTATCCTGTTTATTCTGCCAATCCCGGCGGCGTTCTTTGGTGCCTGGTATGCCGGTCGTGACGG
CAAAAGCTTCCACAGTCTGGTGTGGCATTCGGTGATCAGTTGCCTGTTGGCGGGCCTGATGATGTGGATCCCCGGGTGGT
TTGGCGTGATGAATATCTGGACGCTGATTGTGCCGGCTGCGCTGTTCTTCTTCGGTGCCGGGATGTTATTCCCGCTGGCC
ACTACCGGGGCGATGGAGCCGTTTCCTTATCTGGCCGGTGCGGCCGGCGCATTGGTCGGGGGCATGCAGAACATGGGCTC
CGGCCTGGCGACCTGGCTTTCCGCCATGCTGCCGCAGACCGGGCAGTTCAGCCTCGGCCTGCTGATGTTTGCCATGGCAT
TACTGATTTTGCTGTGCTGGTGGCCGTTATCGCATCGGATGCAGCATCAGGGACATACCGTATAA

Upstream 100 bases:

>100_bases
AATTTGTTAGCTTATGCCGCCATAGAGGGCTGCTCGCCGTTCTGGCTGAATTAGGGGCCATACCGGGACGCTGTGACGAG
TTAATACGTTAACGAAGATA

Downstream 100 bases:

>100_bases
AACTGACAGGGCGCCGAAGGCGCCCTGATTATTTACAGCAGGAACTCGTGCAGCGCCGGGTCTTTACGATCCAGATAGTG
GGTTGAGCGGATACGGCGGA

Product: multidrug resistance protein D

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 394; Mature: 394

Protein sequence:

>394_residues
MRKIENFHLLVMLTLLVAVGQMAQTIYVPVIADIAKDLSVRSGAVQRVMAAYLMTYGFSQLIYGPLSDRIGRRPVILAGM
MIFMMGALGALLSTNLTMLVAASAIQGMGTGVAGVMARTMPRDLYAGTSLRYANSLLNMGILVSPLLAPIIGGALAMIFG
WRACYAFLLVLCAGVAFAMFRWLPETRPQQTEKRRMLASFRKLLADSTFSCYLVMLIGALAGIAVFEASCGVLMGGVLGL
SGLTVSILFILPIPAAFFGAWYAGRDGKSFHSLVWHSVISCLLAGLMMWIPGWFGVMNIWTLIVPAALFFFGAGMLFPLA
TTGAMEPFPYLAGAAGALVGGMQNMGSGLATWLSAMLPQTGQFSLGLLMFAMALLILLCWWPLSHRMQHQGHTV

Sequences:

>Translated_394_residues
MRKIENFHLLVMLTLLVAVGQMAQTIYVPVIADIAKDLSVRSGAVQRVMAAYLMTYGFSQLIYGPLSDRIGRRPVILAGM
MIFMMGALGALLSTNLTMLVAASAIQGMGTGVAGVMARTMPRDLYAGTSLRYANSLLNMGILVSPLLAPIIGGALAMIFG
WRACYAFLLVLCAGVAFAMFRWLPETRPQQTEKRRMLASFRKLLADSTFSCYLVMLIGALAGIAVFEASCGVLMGGVLGL
SGLTVSILFILPIPAAFFGAWYAGRDGKSFHSLVWHSVISCLLAGLMMWIPGWFGVMNIWTLIVPAALFFFGAGMLFPLA
TTGAMEPFPYLAGAAGALVGGMQNMGSGLATWLSAMLPQTGQFSLGLLMFAMALLILLCWWPLSHRMQHQGHTV
>Mature_394_residues
MRKIENFHLLVMLTLLVAVGQMAQTIYVPVIADIAKDLSVRSGAVQRVMAAYLMTYGFSQLIYGPLSDRIGRRPVILAGM
MIFMMGALGALLSTNLTMLVAASAIQGMGTGVAGVMARTMPRDLYAGTSLRYANSLLNMGILVSPLLAPIIGGALAMIFG
WRACYAFLLVLCAGVAFAMFRWLPETRPQQTEKRRMLASFRKLLADSTFSCYLVMLIGALAGIAVFEASCGVLMGGVLGL
SGLTVSILFILPIPAAFFGAWYAGRDGKSFHSLVWHSVISCLLAGLMMWIPGWFGVMNIWTLIVPAALFFFGAGMLFPLA
TTGAMEPFPYLAGAAGALVGGMQNMGSGLATWLSAMLPQTGQFSLGLLMFAMALLILLCWWPLSHRMQHQGHTV

Specific function: Multidrug resistance pump that participates in a low energy shock adaptative response [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily [H]

Homologues:

Organism=Escherichia coli, GI87082312, Length=394, Percent_Identity=72.0812182741117, Blast_Score=521, Evalue=1e-149,
Organism=Escherichia coli, GI48994889, Length=342, Percent_Identity=25.7309941520468, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1788509, Length=356, Percent_Identity=23.876404494382, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1787065, Length=164, Percent_Identity=29.8780487804878, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI1790146, Length=336, Percent_Identity=24.1071428571429, Blast_Score=75, Evalue=9e-15,
Organism=Escherichia coli, GI1790794, Length=185, Percent_Identity=25.4054054054054, Blast_Score=72, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6324264, Length=187, Percent_Identity=26.2032085561497, Blast_Score=73, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 42362; Mature: 42362

Theoretical pI: Translated: 9.87; Mature: 9.87

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
7.6 %Met     (Translated Protein)
9.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
7.6 %Met     (Mature Protein)
9.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCC
>Mature Secondary Structure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CCHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8240355; 7686882; 9278503 [H]