Click here to switch to the map view.

The map label for this gene is metB [H]

Identifier: 157373018

GI number: 157373018

Start: 5305190

End: 5306350

Strand: Reverse

Name: metB [H]

Synonym: Spro_4786

Alternate gene names: 157373018

Gene position: 5306350-5305190 (Counterclockwise)

Preceding gene: 157373020

Following gene: 157373017

Centisome position: 97.38

GC content: 58.31

Gene sequence:

>1161_bases
ATGACGCGTAAACCTGCGACGATTGCCGTACGTAGCGGCCTGAACGATGACGAACAGTACGGCTGCGTTGTCCCGCCGAT
CCACCTTTCCAGCACCTATAATTTCACCGACTTCAACCAGCCTCGTGCTCATGACTACTCACGTCGCGGTAACCCGACGC
GTGACGTGGTCCAGCGTGCGCTGGCGGAACTGGAAGGGGGCGCCGGTGCGGTGATGACCAGCAGCGGGATGTCGGCAATC
CACCTGGTGACCACGGTATTCCTGCAACCCGGCGATCTGCTGGTGGCGCCACATGATTGCTATGGTGGCAGCTACCGTCT
GTTCGACAGCCTGAGCAAGCGTGGTGCTTACCGCGTGCTGTTTGTCGATCAGGGGAATGAAGAAGCGCTGCAGCAGGCGT
TGGCGCAAAAACCCAAGCTGGTGCTGATTGAAAGCCCAAGCAACCCACTGCTGCGTGTGGTGGATATTGCGGCGATTTGC
GATGCGGCACACGCCGTGGGGGCCTTGACGGTGGTGGACAACACCTTCCTCAGCCCGGCGTTACAGCAGCCGATTGGGCT
GGGTGCCGATTTGGTGGTGCATTCCTGTACCAAATACCTCAATGGCCATTCCGACGTGGTGGCCGGCGCGGTGATCGCCA
AAGATAAAGATCTGGCGGTGGAATTGGCGTGGTGGGCCAATAATATCGGCGTGACCGGTGGCGCGTTTGACAGCTATTTG
TTGCTGCGCGGCATGCGCACACTGTCGCCGCGTATCAAGGCAGCGCAACAAAATGCCGATGCGATTGTCAGTTATTTACA
GCAGCAACCCTTGGTGAAAAAGCTGTATCATCCTTCTCTGCCTCAGAACCCTGGGCACGAGATTGCACGTCGTCAGCAAC
GCGGCTTTGGCGCGATGCTCAGCTTTGAACTGGACGGTGATGAAGCGCTATTGCGCCGTTTTCTGTCTGCCCTTGAGCTG
TTTACTCTGGCAGAATCCTTGGGCGGCGTAGAAAGCCTGATCTCGCATGCAGCGACCATGACCCACGCAGGTATGGCGGC
AGAAGCCCGTGCGGCGGCCGGTATTTCCGAGAGCCTGCTGCGTATCTCCGTGGGTATTGAAGACAGTGAAGATTTGATTG
CCGATCTGGAACGAGCCTTCCAGGCGGCGGCAACGAGGTAA

Upstream 100 bases:

>100_bases
TACGCAAAGAAGTTTAGATGTCCAGATGTATTGACGTCCATCCTCGCTGCGATTAATCTTTAGGCCTCATTCATCCCAAT
CCCGAGAGCCGAGAGACCCT

Downstream 100 bases:

>100_bases
GCATGAATGCAATTGCGGTAGCAGGGCCGGTGAGCGGGCGTCAACTACACAAGTTTGGCGGCAGCAGCCTGGCAGACGTG
AAGTGTTATCTGCGGGTGGC

Product: cystathionine gamma-synthase

Products: NA

Alternate protein names: CGS; O-succinylhomoserine (thiol)-lyase [H]

Number of amino acids: Translated: 386; Mature: 385

Protein sequence:

>386_residues
MTRKPATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTDFNQPRAHDYSRRGNPTRDVVQRALAELEGGAGAVMTSSGMSAI
HLVTTVFLQPGDLLVAPHDCYGGSYRLFDSLSKRGAYRVLFVDQGNEEALQQALAQKPKLVLIESPSNPLLRVVDIAAIC
DAAHAVGALTVVDNTFLSPALQQPIGLGADLVVHSCTKYLNGHSDVVAGAVIAKDKDLAVELAWWANNIGVTGGAFDSYL
LLRGMRTLSPRIKAAQQNADAIVSYLQQQPLVKKLYHPSLPQNPGHEIARRQQRGFGAMLSFELDGDEALLRRFLSALEL
FTLAESLGGVESLISHAATMTHAGMAAEARAAAGISESLLRISVGIEDSEDLIADLERAFQAAATR

Sequences:

>Translated_386_residues
MTRKPATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTDFNQPRAHDYSRRGNPTRDVVQRALAELEGGAGAVMTSSGMSAI
HLVTTVFLQPGDLLVAPHDCYGGSYRLFDSLSKRGAYRVLFVDQGNEEALQQALAQKPKLVLIESPSNPLLRVVDIAAIC
DAAHAVGALTVVDNTFLSPALQQPIGLGADLVVHSCTKYLNGHSDVVAGAVIAKDKDLAVELAWWANNIGVTGGAFDSYL
LLRGMRTLSPRIKAAQQNADAIVSYLQQQPLVKKLYHPSLPQNPGHEIARRQQRGFGAMLSFELDGDEALLRRFLSALEL
FTLAESLGGVESLISHAATMTHAGMAAEARAAAGISESLLRISVGIEDSEDLIADLERAFQAAATR
>Mature_385_residues
TRKPATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTDFNQPRAHDYSRRGNPTRDVVQRALAELEGGAGAVMTSSGMSAIH
LVTTVFLQPGDLLVAPHDCYGGSYRLFDSLSKRGAYRVLFVDQGNEEALQQALAQKPKLVLIESPSNPLLRVVDIAAICD
AAHAVGALTVVDNTFLSPALQQPIGLGADLVVHSCTKYLNGHSDVVAGAVIAKDKDLAVELAWWANNIGVTGGAFDSYLL
LRGMRTLSPRIKAAQQNADAIVSYLQQQPLVKKLYHPSLPQNPGHEIARRQQRGFGAMLSFELDGDEALLRRFLSALELF
TLAESLGGVESLISHAATMTHAGMAAEARAAAGISESLLRISVGIEDSEDLIADLERAFQAAATR

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=381, Percent_Identity=40.9448818897638, Blast_Score=265, Evalue=4e-71,
Organism=Homo sapiens, GI299473759, Length=381, Percent_Identity=38.0577427821522, Blast_Score=232, Evalue=4e-61,
Organism=Homo sapiens, GI25453487, Length=380, Percent_Identity=35.5263157894737, Blast_Score=206, Evalue=2e-53,
Organism=Escherichia coli, GI1790375, Length=383, Percent_Identity=85.378590078329, Blast_Score=681, Evalue=0.0,
Organism=Escherichia coli, GI1789383, Length=342, Percent_Identity=32.1637426900585, Blast_Score=157, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI17538055, Length=366, Percent_Identity=45.0819672131148, Blast_Score=299, Evalue=2e-81,
Organism=Caenorhabditis elegans, GI17560056, Length=366, Percent_Identity=43.4426229508197, Blast_Score=288, Evalue=3e-78,
Organism=Caenorhabditis elegans, GI71980708, Length=369, Percent_Identity=34.4173441734417, Blast_Score=224, Evalue=6e-59,
Organism=Saccharomyces cerevisiae, GI6319307, Length=388, Percent_Identity=39.6907216494845, Blast_Score=257, Evalue=2e-69,
Organism=Saccharomyces cerevisiae, GI6321254, Length=396, Percent_Identity=34.3434343434343, Blast_Score=191, Evalue=2e-49,
Organism=Saccharomyces cerevisiae, GI6321904, Length=395, Percent_Identity=29.873417721519, Blast_Score=150, Evalue=4e-37,
Organism=Saccharomyces cerevisiae, GI6321170, Length=322, Percent_Identity=29.8136645962733, Blast_Score=148, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6323333, Length=409, Percent_Identity=29.8288508557457, Blast_Score=142, Evalue=6e-35,
Organism=Saccharomyces cerevisiae, GI6322590, Length=279, Percent_Identity=27.5985663082437, Blast_Score=78, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6322970, Length=271, Percent_Identity=26.9372693726937, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI19922554, Length=382, Percent_Identity=41.8848167539267, Blast_Score=301, Evalue=5e-82,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR011821
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =2.5.1.48 [H]

Molecular weight: Translated: 41440; Mature: 41308

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRKPATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTDFNQPRAHDYSRRGNPTRDVVQRA
CCCCCCEEEEECCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
LAELEGGAGAVMTSSGMSAIHLVTTVFLQPGDLLVAPHDCYGGSYRLFDSLSKRGAYRVL
HHHHCCCCCEEEECCCCHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHCCCEEEE
FVDQGNEEALQQALAQKPKLVLIESPSNPLLRVVDIAAICDAAHAVGALTVVDNTFLSPA
EEECCCHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHEECCCHHHCHH
LQQPIGLGADLVVHSCTKYLNGHSDVVAGAVIAKDKDLAVELAWWANNIGVTGGAFDSYL
HHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHEECCCCCEEEEEEECCCCCCCCCCHHHHH
LLRGMRTLSPRIKAAQQNADAIVSYLQQQPLVKKLYHPSLPQNPGHEIARRQQRGFGAML
HHHHHHHHCHHHHHHHCCHHHHHHHHHHCHHHHHHCCCCCCCCCCHHHHHHHHCCCCEEE
SFELDGDEALLRRFLSALELFTLAESLGGVESLISHAATMTHAGMAAEARAAAGISESLL
EEEECCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHE
RISVGIEDSEDLIADLERAFQAAATR
EEEECCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TRKPATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTDFNQPRAHDYSRRGNPTRDVVQRA
CCCCCEEEEECCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
LAELEGGAGAVMTSSGMSAIHLVTTVFLQPGDLLVAPHDCYGGSYRLFDSLSKRGAYRVL
HHHHCCCCCEEEECCCCHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHCCCEEEE
FVDQGNEEALQQALAQKPKLVLIESPSNPLLRVVDIAAICDAAHAVGALTVVDNTFLSPA
EEECCCHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHEECCCHHHCHH
LQQPIGLGADLVVHSCTKYLNGHSDVVAGAVIAKDKDLAVELAWWANNIGVTGGAFDSYL
HHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHEECCCCCEEEEEEECCCCCCCCCCHHHHH
LLRGMRTLSPRIKAAQQNADAIVSYLQQQPLVKKLYHPSLPQNPGHEIARRQQRGFGAML
HHHHHHHHCHHHHHHHCCHHHHHHHHHHCHHHHHHCCCCCCCCCCHHHHHHHHCCCCEEE
SFELDGDEALLRRFLSALELFTLAESLGGVESLISHAATMTHAGMAAEARAAAGISESLL
EEEECCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHE
RISVGIEDSEDLIADLERAFQAAATR
EEEECCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6361020; 8346018; 9278503; 3307782; 9843488 [H]