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The map label for this gene is nylA [H]

Identifier: 157372942

GI number: 157372942

Start: 5204613

End: 5206058

Strand: Reverse

Name: nylA [H]

Synonym: Spro_4710

Alternate gene names: 157372942

Gene position: 5206058-5204613 (Counterclockwise)

Preceding gene: 157372944

Following gene: 157372941

Centisome position: 95.54

GC content: 61.76

Gene sequence:

>1446_bases
ATGATGAAATTGAGCGAGTACGTTAGCTATGATGCGCTGGGGTTGGCCGAACTGGTACAGCGCGGCGAAGTCAGTGCGCA
AGAATTGCAGCAGACGGCGCAGGCCGCGGTTGAGGCGGTGAACCCGGCGATTAACGCAGTGGTGGAACATTGGACGCAGC
CGCTATCGGAGGGGGAAGGCCCACTGTCTGGCGTGCCTTTCCTGATTAAGGATATCGCCATCAGCATGGCCGGTAAGCTC
TCGGAACTGGGCAGCCGGTTGGCGCAGGGTAATATTGCCACTACAGACAGTTATCTGATGAGCAATATTCGTCGAGCAGG
GCTGGTCACTTTCGGTCGCACTACCACGCCGGAAATGGCGTTCAGCACCACCACCGAAGCGGTGCTTTACGGCGCGACGC
GCAATCCTTGGAACCCGGATTTCAGCGCCGGCGGTTCCAGCGGCGGAGCCGGTGCGGCGGTGGCTGCCGGGATCGTGCCG
ATTGCTCACGCTACGGACGCCGCCGGCTCGATCCGCGTACCCGCTTCGTCGAATGGGCTATTTGGCCTCAAACCGACGCG
TGGCCGGGTATCGCATGGCCCGGCGATGGATGAAGTGTTCAACGGTTTTGGCGTCCAACTGGGGCTAAGCCGTTCGGTAC
GTGACAGCGCGGCCTTGCTGGATGCCCTGCAGCATCCACATCCCGGCGAACCCTATTACACCACGCCTCCGGCACAGAGT
TGGCTGTCACAGGTGACGTCAGAACCCGGCCGACTGCGCATCGGCATGATGACTCAGGCGTGGAACGGCGGCAAAACCGA
TCCGCAAATCGCTCAGGCCACGGCCGACACTGGCGTGCTGCTGAGTGAGTTGGGCCATCAGGTTAGCGAAGTGGATATGG
CGATCGGCGTCAGTTGGCAGGAGCTGGTGTTCGCCAATGCGCAAATCTGGTGTGCCAATCTGGTCGGTTGGCTAGATGGC
CTGAGCCAGGCGACCGGGCGGCCGATTTCAACCGAAACGCTGGAGCCGGAAACGCTGGCCTGTTACCGCTACGGTTTGGC
TGCCAGGGCGGTGGATATGGTCGCTGCGCTTGAGGTGCGTAATCGGGTGACTCGCGGTGTCGCGGCTTATTTTCAGCAGT
ATGACCTGCTGCTGACCCCAACTCTGCCCGATTTGCCCTGGCGGATTGGCCGTTACGGCACAGGAGCGGCGGAGATGAGC
GGCCTGGAGTGGACGGCGCATTTATTCGATCACTCGCCGTTTACGCCGGTCTTTAACGTGGCGGGCGTGCCGGCGATGTC
GGTGCCGTTGGCGCATGATGCGCAGCATAACCTGCCGATTGGCATGCAATTCGTCGCCGGTTTTGGCCGTGAAGATTTGC
TGCTGCAACTGGCCGGCCAACTGGAGCGGGCGGCACCCTGGCAGCAGCGACGTCCTGCGATTTGGGCGGGAAATGCACGG
CCGTAG

Upstream 100 bases:

>100_bases
ATTAAATCTATCAATTTCAACTATAGCGTTAATCCCACTACCGTGGAACGGACGGCAGAAAAGCCGACGAAACTGCTACG
GATCTACGGATAAGGACGAA

Downstream 100 bases:

>100_bases
CAGTCAAAAGGCAAAATATCGGCTATGTTGCTAAAAGGTTTTTTGCGGCGGGCCGTCGGCTCGTCGCTGGATTCAGGAGG
CGTGATGAGCTGCATATTTT

Product: amidase

Products: NA

Alternate protein names: Nylon oligomers-degrading enzyme EI [H]

Number of amino acids: Translated: 481; Mature: 481

Protein sequence:

>481_residues
MMKLSEYVSYDALGLAELVQRGEVSAQELQQTAQAAVEAVNPAINAVVEHWTQPLSEGEGPLSGVPFLIKDIAISMAGKL
SELGSRLAQGNIATTDSYLMSNIRRAGLVTFGRTTTPEMAFSTTTEAVLYGATRNPWNPDFSAGGSSGGAGAAVAAGIVP
IAHATDAAGSIRVPASSNGLFGLKPTRGRVSHGPAMDEVFNGFGVQLGLSRSVRDSAALLDALQHPHPGEPYYTTPPAQS
WLSQVTSEPGRLRIGMMTQAWNGGKTDPQIAQATADTGVLLSELGHQVSEVDMAIGVSWQELVFANAQIWCANLVGWLDG
LSQATGRPISTETLEPETLACYRYGLAARAVDMVAALEVRNRVTRGVAAYFQQYDLLLTPTLPDLPWRIGRYGTGAAEMS
GLEWTAHLFDHSPFTPVFNVAGVPAMSVPLAHDAQHNLPIGMQFVAGFGREDLLLQLAGQLERAAPWQQRRPAIWAGNAR
P

Sequences:

>Translated_481_residues
MMKLSEYVSYDALGLAELVQRGEVSAQELQQTAQAAVEAVNPAINAVVEHWTQPLSEGEGPLSGVPFLIKDIAISMAGKL
SELGSRLAQGNIATTDSYLMSNIRRAGLVTFGRTTTPEMAFSTTTEAVLYGATRNPWNPDFSAGGSSGGAGAAVAAGIVP
IAHATDAAGSIRVPASSNGLFGLKPTRGRVSHGPAMDEVFNGFGVQLGLSRSVRDSAALLDALQHPHPGEPYYTTPPAQS
WLSQVTSEPGRLRIGMMTQAWNGGKTDPQIAQATADTGVLLSELGHQVSEVDMAIGVSWQELVFANAQIWCANLVGWLDG
LSQATGRPISTETLEPETLACYRYGLAARAVDMVAALEVRNRVTRGVAAYFQQYDLLLTPTLPDLPWRIGRYGTGAAEMS
GLEWTAHLFDHSPFTPVFNVAGVPAMSVPLAHDAQHNLPIGMQFVAGFGREDLLLQLAGQLERAAPWQQRRPAIWAGNAR
P
>Mature_481_residues
MMKLSEYVSYDALGLAELVQRGEVSAQELQQTAQAAVEAVNPAINAVVEHWTQPLSEGEGPLSGVPFLIKDIAISMAGKL
SELGSRLAQGNIATTDSYLMSNIRRAGLVTFGRTTTPEMAFSTTTEAVLYGATRNPWNPDFSAGGSSGGAGAAVAAGIVP
IAHATDAAGSIRVPASSNGLFGLKPTRGRVSHGPAMDEVFNGFGVQLGLSRSVRDSAALLDALQHPHPGEPYYTTPPAQS
WLSQVTSEPGRLRIGMMTQAWNGGKTDPQIAQATADTGVLLSELGHQVSEVDMAIGVSWQELVFANAQIWCANLVGWLDG
LSQATGRPISTETLEPETLACYRYGLAARAVDMVAALEVRNRVTRGVAAYFQQYDLLLTPTLPDLPWRIGRYGTGAAEMS
GLEWTAHLFDHSPFTPVFNVAGVPAMSVPLAHDAQHNLPIGMQFVAGFGREDLLLQLAGQLERAAPWQQRRPAIWAGNAR
P

Specific function: Specifically catalyzes the hydrolysis of 6-aminohexanoic acid cyclic dimer (1,8-diazacyclotetradecane-2,9-dione) to form the linear dimer 6-aminohexanoyl-6-aminohexanoic acid. Is inactive on 6-aminohexanoic acid oligomers (degree of polymerization 2 to 6)

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI195972892, Length=196, Percent_Identity=30.6122448979592, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI222831590, Length=509, Percent_Identity=24.3614931237721, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI166795287, Length=260, Percent_Identity=31.5384615384615, Blast_Score=69, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI17538252, Length=306, Percent_Identity=26.4705882352941, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17556264, Length=289, Percent_Identity=30.4498269896194, Blast_Score=86, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17537465, Length=196, Percent_Identity=29.5918367346939, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71990152, Length=259, Percent_Identity=30.5019305019305, Blast_Score=71, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17556276, Length=208, Percent_Identity=27.8846153846154, Blast_Score=65, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI17556278, Length=208, Percent_Identity=27.8846153846154, Blast_Score=65, Evalue=9e-11,
Organism=Saccharomyces cerevisiae, GI6319685, Length=408, Percent_Identity=26.7156862745098, Blast_Score=70, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6320448, Length=144, Percent_Identity=38.8888888888889, Blast_Score=68, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24644968, Length=482, Percent_Identity=25.9336099585062, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24652985, Length=511, Percent_Identity=25.440313111546, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI19922090, Length=511, Percent_Identity=25.440313111546, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24652981, Length=511, Percent_Identity=25.440313111546, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24652983, Length=511, Percent_Identity=25.440313111546, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI21356731, Length=241, Percent_Identity=28.6307053941909, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI45550774, Length=246, Percent_Identity=27.6422764227642, Blast_Score=92, Evalue=9e-19,
Organism=Drosophila melanogaster, GI24648435, Length=246, Percent_Identity=27.6422764227642, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24648437, Length=246, Percent_Identity=27.6422764227642, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI161078093, Length=500, Percent_Identity=26.2, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24648113, Length=449, Percent_Identity=27.3942093541203, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24648441, Length=170, Percent_Identity=31.1764705882353, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24648439, Length=170, Percent_Identity=31.1764705882353, Blast_Score=78, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: =3.5.2.12 [H]

Molecular weight: Translated: 51194; Mature: 51194

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKLSEYVSYDALGLAELVQRGEVSAQELQQTAQAAVEAVNPAINAVVEHWTQPLSEGEG
CCCHHHHHCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PLSGVPFLIKDIAISMAGKLSELGSRLAQGNIATTDSYLMSNIRRAGLVTFGRTTTPEMA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCEEEECCCCCCCHH
FSTTTEAVLYGATRNPWNPDFSAGGSSGGAGAAVAAGIVPIAHATDAAGSIRVPASSNGL
HCCCCCCEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEECCCCCCCE
FGLKPTRGRVSHGPAMDEVFNGFGVQLGLSRSVRDSAALLDALQHPHPGEPYYTTPPAQS
EECCCCCCCCCCCCCHHHHHCCCCEEEECCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHH
WLSQVTSEPGRLRIGMMTQAWNGGKTDPQIAQATADTGVLLSELGHQVSEVDMAIGVSWQ
HHHHHHCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCHH
ELVFANAQIWCANLVGWLDGLSQATGRPISTETLEPETLACYRYGLAARAVDMVAALEVR
HHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
NRVTRGVAAYFQQYDLLLTPTLPDLPWRIGRYGTGAAEMSGLEWTAHLFDHSPFTPVFNV
HHHHHHHHHHHHHCCEEECCCCCCCCHHHCCCCCCHHHHCCCHHHHHHHCCCCCCCHHHC
AGVPAMSVPLAHDAQHNLPIGMQFVAGFGREDLLLQLAGQLERAAPWQQRRPAIWAGNAR
CCCCCCCCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHCCCHHHCCCCEECCCCC
P
C
>Mature Secondary Structure
MMKLSEYVSYDALGLAELVQRGEVSAQELQQTAQAAVEAVNPAINAVVEHWTQPLSEGEG
CCCHHHHHCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PLSGVPFLIKDIAISMAGKLSELGSRLAQGNIATTDSYLMSNIRRAGLVTFGRTTTPEMA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCEEEECCCCCCCHH
FSTTTEAVLYGATRNPWNPDFSAGGSSGGAGAAVAAGIVPIAHATDAAGSIRVPASSNGL
HCCCCCCEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEECCCCCCCE
FGLKPTRGRVSHGPAMDEVFNGFGVQLGLSRSVRDSAALLDALQHPHPGEPYYTTPPAQS
EECCCCCCCCCCCCCHHHHHCCCCEEEECCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHH
WLSQVTSEPGRLRIGMMTQAWNGGKTDPQIAQATADTGVLLSELGHQVSEVDMAIGVSWQ
HHHHHHCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCHH
ELVFANAQIWCANLVGWLDGLSQATGRPISTETLEPETLACYRYGLAARAVDMVAALEVR
HHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
NRVTRGVAAYFQQYDLLLTPTLPDLPWRIGRYGTGAAEMSGLEWTAHLFDHSPFTPVFNV
HHHHHHHHHHHHHCCEEECCCCCCCCHHHCCCCCCHHHHCCCHHHHHHHCCCCCCCHHHC
AGVPAMSVPLAHDAQHNLPIGMQFVAGFGREDLLLQLAGQLERAAPWQQRRPAIWAGNAR
CCCCCCCCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHCCCHHHCCCCEECCCCC
P
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2722746 [H]