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The map label for this gene is pitA [H]

Identifier: 157372921

GI number: 157372921

Start: 5180729

End: 5182231

Strand: Direct

Name: pitA [H]

Synonym: Spro_4689

Alternate gene names: 157372921

Gene position: 5180729-5182231 (Clockwise)

Preceding gene: 157372919

Following gene: 157372923

Centisome position: 95.08

GC content: 55.02

Gene sequence:

>1503_bases
ATGCTGCATTTGTTCGCTGGCCTGGATTTCCATACCGGCCTGATGTTAATTCTCGCATTGTTGTTCGTGTTGTTTTATGA
AGCCATTAATGGTTTTCATGATACGGCCAATGCGGTTGCTACAGTAATTTATACCCGTGCCATGCGCTCGCAACTTGCGG
TCGTGATGGCTGGTTTGTTCAACTTTCTTGGCGTGATGCTCGGTGGTTTGAGTGTTGCTTACGCCATTGTTCACCTGTTG
CCAACAGATCTGTTGTTGAACGTCAGTTCAGCACACGGATTAGCCATGGTCTTTTCCATGCTGCTGGCGGCTATCATCTG
GAACCTCGGCACCTGGTATTTCGGTCTGCCGGCCTCCAGTTCTCATACGCTGATTGGCGCAATTATTGGTGTGGGTTTGA
CCAACGCACTGATGACCCACACTTCGGTGGTGGATGCGCTTAACGTCCCGAAAATGATTGGTATTTTCCTGTCATTGCTC
ATCTCGCCACTGGTTGGCATGATGGTCGCCGGGTTGATGGTGTTTGCCTTGCGCCGCTATTGGAGTGGCACCAAGAAACG
CCAGCGTATCCACATGACCCCTGCTGAACGTGAAAAGGTCGACGGCAAACGCAAGCCGCCGTTCTGGACCCGTATCGCTC
TGATCCTGTCGGCTATCGGCGTTAGCTTCTCGCACGGCGCCAACGACGGCCAGAAAGGTATCGGCTTGATTATGCTGGTG
CTGATCGGCGTTGCGCCGGCCGGCTTCGTGCTCAATATGAATGCAACCGGTTATGACATCACCCGTACTCGTGATGCCGT
GGCCCACCTGCAGCAATATTATGAGCAGCACAGCGATGCCCTGTCGCACGTGGTCGCTCTCACTCCGTTGGTGCCAAGCC
CGGATGAGGACGTCTCGCCGGATAAACCCGTCGAGTTCCACTGTGACAGTGCCCGCGCCATGCCGGCAATTGGGCTTGCC
TCCGGGTTGCTGAACAATCTGCAAAGTTACGATCAACTGTCGGTAGATCAGCGCAGCCACCTGCGCCGCCTGCTGATGTG
CGTAACAGACACCGCAGACAAGATTGCCAAGCTGCCGGAAACCTCAAGCCAGGACCAACGTTTCCTGAAAAACCTGCGTC
AGGATCTGCTGCAAACCGTTGAGTATGCACCGATCTGGATTATCGTCGCGGTTGCTCTGGCGTTGTCACTCGGCACCATG
GTCGGCTGGCAGCGTGTAGCCACCACCATTGGCGAGAAGATCGGTAAGAAGGGCATGACCTACGCTCAGGGCGTGTCGGC
TCAGATGACCGCAGCCTTGTCGATTGGTGTCGCCAGTTATACCGGTATGCCGGTTTCCACCACCCACGTGCTTTCTTCGG
CGGTAGCCGGGACCATGATCGTGGACGGCGGCGGCGTACAAAGCAAAACCGTGAAGAATATCCTGTTGGCCTGGTTGTTG
ACCCTGCCGATTTCGATTCTGCTTTCCGGTTCGCTGTACTGGTTGGCGCTTAAGGTGATCTAA

Upstream 100 bases:

>100_bases
GTCATGTCCAAATCAAAAAAACGTCATATTTTCCTTTTCCCCGGGCCCGGTTGTCACGGATAATGCGCGGCGTTCATGTC
CACAAACTGGCGTAACGCTT

Downstream 100 bases:

>100_bases
GCAGTTTGCTCCATATGAAAGGCGGCCTAAATGGCCGCCTTTTCTTTATCTGTTACAGGGCAAACAGGCCGGGCTTAATA
CCAGATCATCAACGCCAGCA

Product: phosphate transporter

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 500; Mature: 500

Protein sequence:

>500_residues
MLHLFAGLDFHTGLMLILALLFVLFYEAINGFHDTANAVATVIYTRAMRSQLAVVMAGLFNFLGVMLGGLSVAYAIVHLL
PTDLLLNVSSAHGLAMVFSMLLAAIIWNLGTWYFGLPASSSHTLIGAIIGVGLTNALMTHTSVVDALNVPKMIGIFLSLL
ISPLVGMMVAGLMVFALRRYWSGTKKRQRIHMTPAEREKVDGKRKPPFWTRIALILSAIGVSFSHGANDGQKGIGLIMLV
LIGVAPAGFVLNMNATGYDITRTRDAVAHLQQYYEQHSDALSHVVALTPLVPSPDEDVSPDKPVEFHCDSARAMPAIGLA
SGLLNNLQSYDQLSVDQRSHLRRLLMCVTDTADKIAKLPETSSQDQRFLKNLRQDLLQTVEYAPIWIIVAVALALSLGTM
VGWQRVATTIGEKIGKKGMTYAQGVSAQMTAALSIGVASYTGMPVSTTHVLSSAVAGTMIVDGGGVQSKTVKNILLAWLL
TLPISILLSGSLYWLALKVI

Sequences:

>Translated_500_residues
MLHLFAGLDFHTGLMLILALLFVLFYEAINGFHDTANAVATVIYTRAMRSQLAVVMAGLFNFLGVMLGGLSVAYAIVHLL
PTDLLLNVSSAHGLAMVFSMLLAAIIWNLGTWYFGLPASSSHTLIGAIIGVGLTNALMTHTSVVDALNVPKMIGIFLSLL
ISPLVGMMVAGLMVFALRRYWSGTKKRQRIHMTPAEREKVDGKRKPPFWTRIALILSAIGVSFSHGANDGQKGIGLIMLV
LIGVAPAGFVLNMNATGYDITRTRDAVAHLQQYYEQHSDALSHVVALTPLVPSPDEDVSPDKPVEFHCDSARAMPAIGLA
SGLLNNLQSYDQLSVDQRSHLRRLLMCVTDTADKIAKLPETSSQDQRFLKNLRQDLLQTVEYAPIWIIVAVALALSLGTM
VGWQRVATTIGEKIGKKGMTYAQGVSAQMTAALSIGVASYTGMPVSTTHVLSSAVAGTMIVDGGGVQSKTVKNILLAWLL
TLPISILLSGSLYWLALKVI
>Mature_500_residues
MLHLFAGLDFHTGLMLILALLFVLFYEAINGFHDTANAVATVIYTRAMRSQLAVVMAGLFNFLGVMLGGLSVAYAIVHLL
PTDLLLNVSSAHGLAMVFSMLLAAIIWNLGTWYFGLPASSSHTLIGAIIGVGLTNALMTHTSVVDALNVPKMIGIFLSLL
ISPLVGMMVAGLMVFALRRYWSGTKKRQRIHMTPAEREKVDGKRKPPFWTRIALILSAIGVSFSHGANDGQKGIGLIMLV
LIGVAPAGFVLNMNATGYDITRTRDAVAHLQQYYEQHSDALSHVVALTPLVPSPDEDVSPDKPVEFHCDSARAMPAIGLA
SGLLNNLQSYDQLSVDQRSHLRRLLMCVTDTADKIAKLPETSSQDQRFLKNLRQDLLQTVEYAPIWIIVAVALALSLGTM
VGWQRVATTIGEKIGKKGMTYAQGVSAQMTAALSIGVASYTGMPVSTTHVLSSAVAGTMIVDGGGVQSKTVKNILLAWLL
TLPISILLSGSLYWLALKVI

Specific function: Low-affinity inorganic phosphate transport. Can also transport arsenate [H]

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family. Pit subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789907, Length=501, Percent_Identity=73.8522954091816, Blast_Score=749, Evalue=0.0,
Organism=Escherichia coli, GI1789360, Length=501, Percent_Identity=69.061876247505, Blast_Score=688, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25146401, Length=514, Percent_Identity=22.7626459143969, Blast_Score=66, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001204 [H]

Pfam domain/function: PF01384 PHO4 [H]

EC number: NA

Molecular weight: Translated: 53848; Mature: 53848

Theoretical pI: Translated: 9.48; Mature: 9.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLHLFAGLDFHTGLMLILALLFVLFYEAINGFHDTANAVATVIYTRAMRSQLAVVMAGLF
CEEEEECCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NFLGVMLGGLSVAYAIVHLLPTDLLLNVSSAHGLAMVFSMLLAAIIWNLGTWYFGLPASS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCEEECCCCC
SHTLIGAIIGVGLTNALMTHTSVVDALNVPKMIGIFLSLLISPLVGMMVAGLMVFALRRY
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WSGTKKRQRIHMTPAEREKVDGKRKPPFWTRIALILSAIGVSFSHGANDGQKGIGLIMLV
HCCCCCCCEECCCCHHHHHCCCCCCCCHHHHHHHHHHHHCCHHCCCCCCCCHHHHHHHHH
LIGVAPAGFVLNMNATGYDITRTRDAVAHLQQYYEQHSDALSHVVALTPLVPSPDEDVSP
HHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
DKPVEFHCDSARAMPAIGLASGLLNNLQSYDQLSVDQRSHLRRLLMCVTDTADKIAKLPE
CCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCHHHHHHCCC
TSSQDQRFLKNLRQDLLQTVEYAPIWIIVAVALALSLGTMVGWQRVATTIGEKIGKKGMT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
YAQGVSAQMTAALSIGVASYTGMPVSTTHVLSSAVAGTMIVDGGGVQSKTVKNILLAWLL
HHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCEEEECCCCCCHHHHHHHHHHHHH
TLPISILLSGSLYWLALKVI
HHHHHHHHCCCHHHHEEECC
>Mature Secondary Structure
MLHLFAGLDFHTGLMLILALLFVLFYEAINGFHDTANAVATVIYTRAMRSQLAVVMAGLF
CEEEEECCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NFLGVMLGGLSVAYAIVHLLPTDLLLNVSSAHGLAMVFSMLLAAIIWNLGTWYFGLPASS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCEEECCCCC
SHTLIGAIIGVGLTNALMTHTSVVDALNVPKMIGIFLSLLISPLVGMMVAGLMVFALRRY
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WSGTKKRQRIHMTPAEREKVDGKRKPPFWTRIALILSAIGVSFSHGANDGQKGIGLIMLV
HCCCCCCCEECCCCHHHHHCCCCCCCCHHHHHHHHHHHHCCHHCCCCCCCCHHHHHHHHH
LIGVAPAGFVLNMNATGYDITRTRDAVAHLQQYYEQHSDALSHVVALTPLVPSPDEDVSP
HHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
DKPVEFHCDSARAMPAIGLASGLLNNLQSYDQLSVDQRSHLRRLLMCVTDTADKIAKLPE
CCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCHHHHHHCCC
TSSQDQRFLKNLRQDLLQTVEYAPIWIIVAVALALSLGTMVGWQRVATTIGEKIGKKGMT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
YAQGVSAQMTAALSIGVASYTGMPVSTTHVLSSAVAGTMIVDGGGVQSKTVKNILLAWLL
HHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCEEEECCCCCCHHHHHHHHHHHHH
TLPISILLSGSLYWLALKVI
HHHHHHHHCCCHHHHEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]