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The map label for this gene is papD [H]

Identifier: 157372906

GI number: 157372906

Start: 5165710

End: 5166462

Strand: Direct

Name: papD [H]

Synonym: Spro_4674

Alternate gene names: 157372906

Gene position: 5165710-5166462 (Clockwise)

Preceding gene: 157372905

Following gene: 157372907

Centisome position: 94.8

GC content: 57.5

Gene sequence:

>753_bases
ATGATGAACCACTTTCCGTTAGCAAAAATGGCCTCGCTGGGCCTGGCATTGGCCGCCGCAGCCCCCGTCGCCAACGCGGC
GATATCGCTGGATCGCACCCGTGCCGTTTACCTCAGCGACGCGAAATCGATCAGCCTGAATATCGTCAATGAAAACAAGG
AACTGCCCTTCCTGGCGCAGTCCTGGCTGGAAAACGAGCAGCACCAGAAAGTCACCTCACCGCTGGTGGTATTGCCGCCA
TTGCAACGGGTTGAGGCCAGCGAACGCAGTGTGGTGCGTATCACCAAGACGCCGGAGGCCGATCGCCTGCCACAGGATCG
CGAATCGGTGTTCTATTTCAACCTGCGAGAGATCCCGCCGAAAAGCAGCAAAACCAATGTGATGCAGTTGGCGTTGCAAA
CCCAGATCAAACTGTTTTATCGCCCGAAGGCCATCGTCGCGCCTAAAGGCGAAGTGTGGCAGGAGAAGCTGGTGTTTCAC
AAAAGCGCCGGCGGTTTTACCGTTGAGAACCCAACGCCGTTTTATATCACCCTGACCGGTATGACCCGTCAGACGCAGAA
ACAGGGCGGCGGCGCACTAGCTGGCTTCCAGCCGGTGATGCTGAAGCCGAAATCCAGCGAGCCCCTCAAGCTGAGCGACA
CTGGCTTCAACGGCTTTGTCGTCAGCTATATCAATGACTACGGCGGCCATCCGGAGCTGCGCTTCGCCTGCAACGGCAGC
GTCTGCAACGCCGTGCCCGATAGCAAAAAATAA

Upstream 100 bases:

>100_bases
CAGCGTCAGTGCCGCATTGTCATACCGGGCGCCGCCAAGCCGCTGGATCAGCTCTTGCTGCCGTGCGCCAAACTGTAATC
CGTAACGCAGAGAATTGAAG

Downstream 100 bases:

>100_bases
GGAGATAGCGCATGGCTGAATTCACCGGCGGCCGCCTGCGGCCCCTGCTGCTCGGCGCGTTGCTGTTCACCGGCAGCGCA
ACGGCGGCGACGCCACTGGG

Product: pili assembly chaperone

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 250; Mature: 250

Protein sequence:

>250_residues
MMNHFPLAKMASLGLALAAAAPVANAAISLDRTRAVYLSDAKSISLNIVNENKELPFLAQSWLENEQHQKVTSPLVVLPP
LQRVEASERSVVRITKTPEADRLPQDRESVFYFNLREIPPKSSKTNVMQLALQTQIKLFYRPKAIVAPKGEVWQEKLVFH
KSAGGFTVENPTPFYITLTGMTRQTQKQGGGALAGFQPVMLKPKSSEPLKLSDTGFNGFVVSYINDYGGHPELRFACNGS
VCNAVPDSKK

Sequences:

>Translated_250_residues
MMNHFPLAKMASLGLALAAAAPVANAAISLDRTRAVYLSDAKSISLNIVNENKELPFLAQSWLENEQHQKVTSPLVVLPP
LQRVEASERSVVRITKTPEADRLPQDRESVFYFNLREIPPKSSKTNVMQLALQTQIKLFYRPKAIVAPKGEVWQEKLVFH
KSAGGFTVENPTPFYITLTGMTRQTQKQGGGALAGFQPVMLKPKSSEPLKLSDTGFNGFVVSYINDYGGHPELRFACNGS
VCNAVPDSKK
>Mature_250_residues
MMNHFPLAKMASLGLALAAAAPVANAAISLDRTRAVYLSDAKSISLNIVNENKELPFLAQSWLENEQHQKVTSPLVVLPP
LQRVEASERSVVRITKTPEADRLPQDRESVFYFNLREIPPKSSKTNVMQLALQTQIKLFYRPKAIVAPKGEVWQEKLVFH
KSAGGFTVENPTPFYITLTGMTRQTQKQGGGALAGFQPVMLKPKSSEPLKLSDTGFNGFVVSYINDYGGHPELRFACNGS
VCNAVPDSKK

Specific function: Binds and caps interactive surfaces on pilus subunits to prevent them from participating in non-productive interactions. Facilitates the import of subunits into the periplasm. May facilitate subunit folding. Chaperone-subunit complexes are then targeted t

COG id: COG3121

COG function: function code NU; P pilus assembly protein, chaperone PapD

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Ig-like (immunoglobulin-like) domain [H]

Homologues:

Organism=Escherichia coli, GI1788677, Length=217, Percent_Identity=45.1612903225806, Blast_Score=206, Evalue=2e-54,
Organism=Escherichia coli, GI1786936, Length=233, Percent_Identity=37.7682403433476, Blast_Score=164, Evalue=4e-42,
Organism=Escherichia coli, GI87082203, Length=251, Percent_Identity=34.2629482071713, Blast_Score=146, Evalue=1e-36,
Organism=Escherichia coli, GI1789532, Length=208, Percent_Identity=33.6538461538462, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1790771, Length=212, Percent_Identity=35.377358490566, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1786333, Length=225, Percent_Identity=33.7777777777778, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1787171, Length=228, Percent_Identity=32.0175438596491, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1788428, Length=214, Percent_Identity=26.6355140186916, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1786743, Length=238, Percent_Identity=31.0924369747899, Blast_Score=98, Evalue=5e-22,
Organism=Escherichia coli, GI87081806, Length=239, Percent_Identity=27.6150627615063, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008962
- InterPro:   IPR001829
- InterPro:   IPR016148
- InterPro:   IPR018046
- InterPro:   IPR016147 [H]

Pfam domain/function: PF02753 Pili_assembly_C; PF00345 Pili_assembly_N [H]

EC number: NA

Molecular weight: Translated: 27543; Mature: 27543

Theoretical pI: Translated: 9.99; Mature: 9.99

Prosite motif: PS00635 PILI_CHAPERONE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMNHFPLAKMASLGLALAAAAPVANAAISLDRTRAVYLSDAKSISLNIVNENKELPFLAQ
CCCCCCHHHHHHHHHHHHHHCCHHHHEEEECCCEEEEEECCCEEEEEEEECCCCCCHHHH
SWLENEQHQKVTSPLVVLPPLQRVEASERSVVRITKTPEADRLPQDRESVFYFNLREIPP
HHHCCHHHHHHCCCEEEECCHHHHCCCCCCEEEEECCCCHHCCCCCCCEEEEEEEECCCC
KSSKTNVMQLALQTQIKLFYRPKAIVAPKGEVWQEKLVFHKSAGGFTVENPTPFYITLTG
CCCCCHHHHHHHHHHEEEEECCCEEECCCCHHHHHHHHEEECCCCEEECCCCCEEEEEEC
MTRQTQKQGGGALAGFQPVMLKPKSSEPLKLSDTGFNGFVVSYINDYGGHPELRFACNGS
CCHHHHHCCCCEECCCCEEEECCCCCCCEEEECCCCCCEEEEEEHHCCCCCCEEEEECCC
VCNAVPDSKK
EECCCCCCCC
>Mature Secondary Structure
MMNHFPLAKMASLGLALAAAAPVANAAISLDRTRAVYLSDAKSISLNIVNENKELPFLAQ
CCCCCCHHHHHHHHHHHHHHCCHHHHEEEECCCEEEEEECCCEEEEEEEECCCCCCHHHH
SWLENEQHQKVTSPLVVLPPLQRVEASERSVVRITKTPEADRLPQDRESVFYFNLREIPP
HHHCCHHHHHHCCCEEEECCHHHHCCCCCCEEEEECCCCHHCCCCCCCEEEEEEEECCCC
KSSKTNVMQLALQTQIKLFYRPKAIVAPKGEVWQEKLVFHKSAGGFTVENPTPFYITLTG
CCCCCHHHHHHHHHHEEEEECCCEEECCCCHHHHHHHHEEECCCCEEECCCCCEEEEEEC
MTRQTQKQGGGALAGFQPVMLKPKSSEPLKLSDTGFNGFVVSYINDYGGHPELRFACNGS
CCHHHHHCCCCEECCCCEEEECCCCCCCEEEECCCCCCEEEEEEHHCCCCCCEEEEECCC
VCNAVPDSKK
EECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1357526; 10049807; 2478891; 10446050 [H]